scholarly journals NS1 antigen: A new beam of light in the early diagnosis of dengue infection

2016 ◽  
Vol 9 (12) ◽  
pp. 1212-1214 ◽  
Author(s):  
Muhammad Suleman ◽  
Rani Faryal ◽  
Muhammad Masroor Alam ◽  
Salmaan Sharif ◽  
Shahzad Shaukat ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Dengue Infection ◽  
Ns1 Antigen ◽  
Antigen A

scholarly journals Burden of Dengue and Chikungunya – A Retrospective Study

2021 ◽  
Author(s):  
J.V. Sathish ◽  
Mita D. Wadekar ◽  
S. Jayashree ◽  
C. Pooja
Keyword(s):  
Retrospective Study ◽  
Early Diagnosis ◽  
Dengue Infection ◽  
Control Measures ◽  
Enzyme Linked Immunosorbent Assay ◽  
Age Group ◽  
Capture Elisa ◽  
Igm Antibody ◽  
Ns1 Antigen ◽  
And Control

Arboviral infections like dengue fever and chikungunya are the most common infections that share the same Aedes mosquito vectors. Clinical presentations of these two infections are also similar, especially in initial stages. Non-structural antigen (NS1 Ag)detection for dengue and detection of IgM antibodies by capture ELISA for chikungunya and dengue infection may help in the early diagnosis. Early diagnosis is essential for the treatment and control measures. The present study was conducted to know the burden of dengue and chikungunya. A retrospective study was conducted for a period of 1 year from Dec 2017 to Nov 2018 to know the burden of dengue and chikungunya in Chamarajanagar. Dengue (> 5 days fever) and chikungunya testing was done by IgM antibody capture ELISA kits produced by NIV. Dengue samples (< 5 days fever) were subjected to NS1 antigen detection by microwell enzyme-linked immunosorbent assay (ELISA) from Qualpro diagnostics. The tests were carried out following manufacturer’s instruction. Samples received for dengue NS1 Ag testing was 446, of which, 49(11.0%) were positive and of 730 samples received for IgM antibody, 53 (7.3%) were positive. Age group commonly affected was 0-20 years 44(43.1%). Of 668 samples received for chikungunya test, 86 (12.9%) were positive. Maximum number of cases was seen in age group of 21-40 years 45(52.3%). Males 56(54.9%) were affected higher than female 46(45.1%) in dengue infection while in chikungunya, females 45(52.3%) were more affected than males 41(47.7%). Both infections are high in the month of June and July. Early detection of dengue by NS1 antigen and detection of Ig M antibodies by capture ELISA chikungunya and dengue infection helps in appropriate treatment and initiation of prevention and control measures by community awareness and vector control.

scholarly journals THE STUDY OF DETECTION OF DENGUE CASES BY NS1 ANTIGEN AND IGM ANTIBODY IN RIMS, ADILABAD, INDIA.

2019 ◽  
Vol 3 (11) ◽  
Author(s):  
Dr. Tanajee Zade ◽  
Dr. K. Srinivas ◽  
Dr. Akshay Berad
Keyword(s):  
Early Diagnosis ◽  
Dengue Fever ◽  
Dengue Infection ◽  
Tertiary Care ◽  
High Morbidity ◽  
Care Hospital ◽  
Serum Samples ◽  
Igg Antibody ◽  
Igm Antibody ◽  
Ns1 Antigen

Dengue fever is an acute febrile arboviral disease affecting tropical & subtropical regions of the world. Dengue infection produces a spectrum of clinical illness, ranging from an asymptomatic to its most severe form like dengue haemorrhagic fever and dengue shock syndrome. In view of high morbidity and mortality, it is imperative to have a rapid and sensitive laboratory assay for early detection of the dengue infection. The newer parameter NS1 antigen has gained a lot of interest for early diagnosis of the disease. Detection of non-structural antigen (NS1 Ag), IgM and IgG antibody may help in the early diagnosis. The present study was conducted in a RIMS Adilabad, tertiary care hospital & medical college in the Department of  General Medicine. A total of 100 serum samples were processed from suspected cases of dengue fever by using dengue test for detection of NS1 antigen and IgG antibodies. Platelet counts of all these cases were noted. . Of these 100 subjects 85 were serologically proved to have dengue illness, 57 patients were NS1 antigen positive, 28 patients were IgM antibody positive patients. As the NS1 antigen is detectable in blood from day one after onset of fever, its assay is an effective tool for early diagnosis of dengue infection so as to avoid complications. Key words:  Dengue, NS1 Antigen, IgM antibody, Platelet

scholarly journals Correlation of serological markers and thrombocytopenia in early diagnosis of dengue infection

2018 ◽  
Vol 6 (3) ◽  
pp. 812 ◽  
Author(s):  
Suchita Vikas Ingale ◽  
Aditi Jayant Upadhye ◽  
Jayshree Jayant Upadhye
Keyword(s):  
Platelet Count ◽  
Early Diagnosis ◽  
Indian Subcontinent ◽  
Secondary Infection ◽  
Dengue Infection ◽  
Supportive Therapy ◽  
Hemorrhagic Fever ◽  
Control Measures ◽  
Serum Samples ◽  
Ns1 Antigen

Background: Dengue is an acute viral infection with fatal complications. To reduce the disease burden, early and specific diagnosis of dengue hemorrhagic fever or dengue shock syndrome with supportive therapy reduces morbidity and mortality. This study was undertaken to evaluate the efficacy of NS1 antigen and platelet count.Methods: A total of 200 serum samples were collected from clinically suspected cases of dengue fever. Serum samples were tested for NS1, IgM and IgG and correlated with the platelet count.Results: Out of 200 samples screened in our study, 100 (50%) were positive for dengue parameters, 100 (50%) were negative. Out of 100 positive cases, 95 (95%) cases were positive for NS1antigen either alone or in combination with antibodies. 84 (84%) cases were exclusively positive for NS1 antigen only. Primary infection (positive for NS1 Ag, IgM, NS1 + IgM) was seen in 99 (99%) cases and secondary infection (positive for IgG, NS1 + IgG, IgM + IgG, NS1+ IgM+ IgG) was seen in 1 case (1%). Out of 100 positive cases, thrombocytopenia was observed in 60 (60%) of patients while 40 (40%) had platelet count within normal range.Conclusions: Dengue is endemic to Indian subcontinent. Currently no specific antiviral therapy is available. Measuring platelet count and detecting the NS1 antigen plays a vital role in early diagnosis, management and implementing the control measures in community to avoid spread of dengue.

Production of IgM Specific Recombinant Dengue Multiepitope Protein for Early Diagnosis of Dengue Infection

2007 ◽  
Vol 23 (2) ◽  
pp. 488-493 ◽  
Author(s):  
N.K. Tripathi ◽  
A. Shrivastva ◽  
P. Pattnaik ◽  
M. Parida ◽  
P.K. Dash ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Dengue Infection

scholarly journals FALSE-NEGATIVE DENGUE CASES IN RORAIMA, BRAZIL: AN APPROACH REGARDING THE HIGH NUMBER OF NEGATIVE RESULTS BY NS1 AG KITS

2014 ◽  
Vol 56 (5) ◽  
pp. 447-450 ◽  
Author(s):  
Pablo O. A. Acosta ◽  
Fabiana Granja ◽  
Cátia A. Meneses ◽  
Ismael A. S. Nascimento ◽  
Débora D. Sousa ◽  
...  
Keyword(s):  
Health Care Providers ◽  
Secondary Infection ◽  
Dengue Infection ◽  
False Negative ◽  
Laboratory Data ◽  
Detection Methods ◽  
Lower Sensitivity ◽  
Care Providers ◽  
Qrt Pcr ◽  
Ns1 Antigen

Serum samples from 150 NS1-negative (Platelia ELISA) patients presumptively diagnosed with dengue were analyzed by the TaqMan probed real-time reverse transcription PCR (TaqMan qRT-PCR) method. The qRT-PCR positive samples were tested for serotype by semi-nested RT-PCR and a qualitative immunochromatographic assay for IgG and IgM. Molecular detection methods showed 33 (22%) positive samples out of 150 NS1-antigen negative samples. Of these, 72% were collected up to day 2 after the onset of symptoms, when diagnostic sensitivity of NS1-antigen test assays is significantly enhanced. Most of the cases were not characterized as secondary infection. Twenty-eight samples were successfully serotyped, 75% of which for DENV-4, 14% for DENV-2, 7% for DENV-3 and 4% for DENV-1. These findings reaffirm the hyperendemic situation of the state of Roraima and suggest a lower sensitivity of the NS1 test, mainly when DENV-4 is the predominant serotype. Health care providers should therefore be aware of samples tested negative by NS1 antigen assays, especially when clinical symptoms and other laboratory data results show evidence of dengue infection.

scholarly journals Correlation of serological markers with haematological parameters in early diagnosis of dengue infection in dengue prone areas

2018 ◽  
Vol 4 (1) ◽  
pp. 76-81
Author(s):  
Dr. Tushar Jalindar Kambale ◽  
◽  
Dr. Komal D Sawaimul ◽  
Dr. M. Banyameen Iqbal ◽  
Dr. Padmakar Bardapurkar ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Dengue Infection ◽  
Haematological Parameters ◽  
Serological Markers
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