Antioxidant Activity of Pinus densiflora Pollen Collected by Apis mellifera

2021 ◽  
Vol 36 (3) ◽  
pp. 177-181
Author(s):  
Hye-Jin Lee ◽  
Sang-Mi Han ◽  
Soon-Ok Woo ◽  
Hyo-Young Kim ◽  
Seonmi Kim ◽  
...  
2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Gabriela Dubiago ◽  
Anna Nowak ◽  
Adam Klimowicz

Honey is produced by bees (Apis mellifera) from flower nectar and insect secretions. Like other bee products it is used as an adjuvant for treatment of many diseases. It also finds its uses for producing cosmetics. The main components of honey include carbohydrates, which represent approximately 80% of the dry matter, and water. Monosaccharides, mainly fructose and glucose, constitute approximately 70% of the sugars, whilst the remaining saccharides are disaccharides and oligosaccharides. Moreover, honey consists vitamins, minerals, polyphenols, amino acids and enzymes. Beneficial effect of natural honey on human health has been known for a long time because of its biological and therapeutic properties. It is used to treat some cardiovascular and gastrointestinal diseases. Among the best-known properties of honey are its antibacterial, antifungal, antiviral and anti-inflammatory activities. Important is also its antioxidant activity, which could be useful for producing anti-aging cosmetics.


2016 ◽  
Vol 31 (3) ◽  
pp. 219 ◽  
Author(s):  
In-Pyo Hong ◽  
Soon-Ok Woo ◽  
Sang-Mi Han ◽  
Se-Gun Kim ◽  
Hye-Ri Jang ◽  
...  

2018 ◽  
Vol 48 (2) ◽  
pp. 158-167 ◽  
Author(s):  
Adelene Menezes Portela BANDEIRA ◽  
Victor Valentim GOMES ◽  
Arthur Abinader VASCONCELOS ◽  
Paulo Sérgio TAUBE ◽  
Emerson Cristi de BARROS ◽  
...  

RESUMO O mel é um produto natural que apresenta várias propriedades benéficas para a saúde, tais como atividade antinflamatória, antioxidante e antimicrobiana, as quais dependem de sua composição. Neste contexto, as propriedades físico-químicas (cor, pH, conteúdo de cinzas, umidade, açúcares e compostos fenólicos totais) e a atividade antioxidadente (capacidade de remoção do radical DPPH) de méis de abelhas Apis mellifera de Santarém, na principal região de produção de mel na Amazônia Oriental, Brasil, foram avaliadas. A maioria das amostras teve cor escura e apresentaram-se ácidas. Os teores de cinzas e a umidade variaram de 0,112 a 0,318 e de 14,751 a 17,514, respectivamente. O teor de açúcares redutores vairou entre 62,873 e 91,563%. O teor total de compostos fenólicos foi mais elevado que os já reportados na literatura, variando entre 15,22 e 16,51 mg g−1 e 17,70 e 18,94 mg g−1 para amostras de mel protegidas e expostas à radiação UV, respectivamente. A quercetina foi encontrada apenas no mel que foi protegido da luz, com teores variando entre 0,24 e 0,43 mg g−1. A ausência de quercetina nas amostras de mel expostas à luz sugere que a radiação UV pode ter degradado esse composto. Todas as amostras apresentaram máxima capacidade de remover o radical DPPH próxima a 50%. Houve correlação inversa entre a cor e o pH, cinzas, açúcares redutores e teor de umidade, e correlação positiva entre a cor e o teor de composto fenólicos e a atividade antioxidante.


2012 ◽  
Vol 17 (2) ◽  
pp. 116-121 ◽  
Author(s):  
Yunyao Jiang ◽  
Woong Han ◽  
Ting Shen ◽  
Myeong-Hyeon Wang

2019 ◽  
Vol 11 ◽  
pp. 51-59
Author(s):  
Samiksha Bhattarai ◽  
Ujjwol Subedi ◽  
Uttam Kumar Bhattarai ◽  
Roman Karki ◽  
Pravin Ojha

Honey samples of commercial honey bee (Apis mellifera) were collected from different bee keepers in Nepal. Total 16 different samples from Dang, Chitwan, Nawalparasi, Sarlahi, Makwanpur and Rautahat districts of Nepal were obtained, representing honey of 4 different floral sources ‘Chiuri’ (Diploknema butyracea), ‘Rudhilo’ (Pogostemon plectranthoides), Mustard (Brassica napus), and Buckwheat (Fagopyrum esculentum). Chemical composition and bioactive components of the honey samples were studied.Moisture content, pH, total acidity of the examined honey samples was found to be in the range of 19.30 ± 0.87 to 20.15 ± 1.39 %, 3.35 ± 0.63 to 4.80 ± 0.15, 109.25 ± 2.06 to 191.25 ± 14.73 meq/kg, respectively. Antioxidant activity, polyphenol, and flavonoid content were found to be in the range of 51.51 ± 4.95 to 97.84 ± 3.75 %, 17.82 ± 1.61 to 59.34 ± 2.77 mg GAE/100g, 1.22 ± 0.65 to 3.86 ± 0.80 mg GAE/100g, respectively. TSS, reducing sugars and HMF content ranged from 77.5 ± 0.46 to 78.0 ± 0.91 oBx, 64.06 ± 1.99 to 70.76 ± 1.26%, and 49.5 ± 4.50 to 214 ± 39.20 mg/kg respectively.


2021 ◽  
Vol 51 (4) ◽  
Author(s):  
Geysa Almeida Viana ◽  
Carlos Iberê Alves Freitas ◽  
José Gustavo Lima de Almeida ◽  
Gerard Vicente Dantas de Medeiros ◽  
Tiago da Silva Teófilo ◽  
...  

ABSTRACT: The objective was to evaluate the in vitro antioxidant, genotoxic, antigenotoxic, and antineoplastic activities of apitoxin produced by the bee Apis mellifera. The antioxidant activity of the apitoxin solution was evaluated using the DPPH (2,2-diphenyl-1-picrilhydrazyl) method. Genotoxic potential of apitoxin was analyzed by comparing the mean DNA damage indices (idDNA) of L929 strain fibroblasts exposed to hydrogen peroxide (H2O2 - genotoxic substance), distilled water, or apitoxin. The antigenotoxic effect of apitoxin was analyzed by assessing the percentage decrease in H2O2-induced genotoxicity in L929 fibroblasts co-treated with three concentrations of the aqueous apitoxin solution and subjected to comet assay. In vitro antineoplastic activity in human tumor cell lines of prostate adenocarcinoma (PC3), hepatocellular carcinoma (HEPGE2), melanoma (MAD-MB435), and astrocytoma (SNB19), were verified by MTT [3- (4) bromide colorimetric method, 5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium]. Apitoxin had no genotoxic effect on L929 cells at concentrations of 30, 10, and 5 µg/mL after 24 hours of exposure. This effect was only evident at 50 µg/mL. Apitoxin promoted a significant reduction in DNA damage index (idDNA) at all concentrations tested. At 30 µg/mL, apitoxin attenuated the genotoxic effects induced by H2O2. Apitoxin also demonstrated in vitro antineoplastic potential, since the cytotoxic effect was observed at concentrations of 50 µg/mL and 25 µg/mL, with significant reduction in viability percentage of PC3 tumor cell lines, HEPGE2, MAD-MB435, and SNB19. The high antioxidant activity associated with the absence of genotoxic effect and the genoprotective and antineoplastic effect demonstrated by apitoxin here provide indications of apitoxin’s therapeutic potential.


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