Effect of trans-10, cis-12 Conjugated Linoleic Acid on Production of Prostaglandin E₂, Cyclooxygenase-2 and 5-lipoxygenase in Lipopolysaccharide-Stimulated Porcine Peripheral Blood Mononuclear Cells

2016 ◽  
Vol 33 (4) ◽  
pp. 194
Author(s):  
Hae-Ryun Seo ◽  
Changhwan Ahn ◽  
Byeong-Teck Kang ◽  
Ji-Houn Kang ◽  
Eui-Bae Jeung ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Cyclooxygenase 2 ◽  
Prostaglandin E ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals Effect of Ciprofloxacin-Induced Prostaglandin E2 on Interleukin-18-Treated Monocytes

2005 ◽  
Vol 49 (8) ◽  
pp. 3228-3233 ◽  
Author(s):  
Hideo Kohka Takahashi ◽  
Hiromi Iwagaki ◽  
Dong Xue ◽  
Goutarou Katsuno ◽  
Sachi Sugita ◽  
...  
Keyword(s):  
Cyclic Amp ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Cyclooxygenase 2 ◽  
Prostaglandin E ◽  
Dependent Manner ◽  
Interleukin 18 ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

ABSTRACT Ciprofloxacin, a fluorinated 4-quinolone, is useful for the clinical treatment of infections due to its antibacterial properties and also modulates the immune response of monocytes isolated from human peripheral blood mononuclear cells. In the present study, we found that ciprofloxacin induced the production of prostaglandin E2 in monocytes in a concentration-dependent manner regardless of the presence of interleukin-18 by enhancing the expression of cyclooxygenase-2 protein and that this in turn led to the elevation of intercellular cyclic AMP in monocytes via the stimulation of prostaglandin receptors. The prostaglandin E2 and cyclic AMP production increased by ciprofloxacin was inhibited by indomethacin, a nonselective cyclooxygenase-2 inhibitor, and NS398, a selective cyclooxygenase-2 inhibitor. In addition, ciprofloxacin suppressed the interleukin-18-induced production of tumor necrosis factor alpha, gamma interferon, and interleukin-12 in peripheral blood mononuclear cells by inhibiting the expression of intercellular adhesion molecule 1, B7.1, B7.2, and CD40 on monocytes, and this effect could be reversed by the addition of indomethacin or NS398. These results indicate that ciprofloxacin exerts immunomodulatory activity via the production of prostaglandin E2 and imply therapeutic potential of ciprofloxacin for the treatment of systemic inflammatory responses initiated by interleukin-18.

scholarly journals Trans-10, cis-12-conjugated linoleic acid modulates NF-κB activation and TNF-α production in porcine peripheral blood mononuclear cells via a PPARγ-dependent pathway

2010 ◽  
Vol 105 (9) ◽  
pp. 1329-1336 ◽  
Author(s):  
Dong-In Kim ◽  
Keun-Hwa Kim ◽  
Ji-Houn Kang ◽  
Eui-Man Jung ◽  
Sung-Soo Kim ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Tnf Α ◽  
Blood Mononuclear Cells ◽  
Cla Isomers ◽  
Dependent Pathway

The activation of PPARγ by ligands, including conjugated linoleic acid (CLA) isomers, plays an important role in the immune response. Among CLA isomers, trans-10, cis-12 (t10c12)-CLA is known to participate in the modulation of pro-inflammatory cytokine secretion. The aim of the present study was to assess the effect of t10c12-CLA on PPARγ activation, NF-κB activation and TNF-α expression in lipopolysaccharide (LPS)-naive and LPS-stimulated porcine peripheral blood mononuclear cells (PBMC). In addition, the effect of PPARγ inhibition on NF-κB activation and TNF-α expression in porcine PBMC was examined. t10c12-CLA was found to increase TNF-α expression and NF-κB activity in LPS-naive porcine PBMC. In contrast, t10c12-CLA decreased TNF-α expression and NF-κB activity in LPS-stimulated porcine PBMC. t10c12-CLA up-regulated PPARγ activity and mRNA expression in both LPS-naive and LPS-stimulated porcine PBMC. GW9662, a PPARγ antagonist, completely negated the modulating effects of t10c12-CLA on TNF-α expression and NF-κB activity in both LPS-naive and LPS-stimulated porcine PBMC. These results suggest that t10c12-CLA can modulate TNF-α production and NF-κB activation by a PPARγ-dependent pathway in porcine PBMC.

scholarly journals Consumption of Cookies Made With a Linoleic Acid-Rich Oil Alters Cardiolipin Species in the Peripheral Blood Mononuclear Cells of Healthy Adults

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 490-490
Author(s):  
Rachel Cole ◽  
Austin Angelotti ◽  
Genevieve Sparagna ◽  
Ai Ni ◽  
Martha Belury
Keyword(s):  
Linoleic Acid ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Healthy Adults ◽  
Controlled Study ◽  
Daily Consumption ◽  
Peripheral Blood Mononuclear ◽  
Significant Difference ◽  
Blood Mononuclear Cells

Abstract Objectives Linoleic acid (LA) is the major fatty acid in cardiolipin (CL), a phospholipid important for mitochondria function. Increasing dietary LA elevates tetralinoleoyl CL (LA4 CL) in cardiac muscle of animals; however, it is not clear if dietary LA increases LA4 CL in humans. The aim of this study was to determine if daily consumption of a cookie made with a LA-rich oil for two weeks could alter LA4 CL in peripheral blood mononuclear cells (PBMC) in healthy adults. Methods In a double-masked placebo-controlled study, 84 healthy adults were randomly assigned to consume one cookie made with LA-rich grapeseed oil (LA-cookie) or one cookie made with oleic acid-rich (OA) safflower oil (OA-cookie), every day for two weeks.  Fasting blood samples were collected for analysis of PBMC cardiolipin species. Results Daily consumption of the LA-cookie for 2 weeks increased LA4 CL in PBMC, while consuming the OA-cookie did not alter LA4 CL (group x week interaction p < 0.01).  LA3OA1 CL decreased in the LA-cookie group but was unchanged in the OA-cookie group (group x week interaction p < 0.01).  There was no significant difference in the change of LA2OA2 CL or LA1OA3 CL between the groups. Conclusions Increasing LA intake through daily cookie consumption increased LA4 CL and altered other CL species in the PBMC of healthy adults.  Future studies are needed to assess if PBMC CL species may be a biomarker for CL species in other tissues and to determine if changes in CL species through increased dietary LA intake are associated with altered mitochondria function. Funding Sources Funding was provided by the Carol S. Kennedy Professorship and the Ohio Agriculture Research and Development Center.

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