scholarly journals DESENVOLVIMENTO DE PRIMERS IN SILICO DE Mycobacterium tuberculosis DA REGIÃO 16S PARA O DIAGNÓSTICO DA TUBERCULOSE

2020 ◽  
Vol 8 (2) ◽  
pp. 77-86
Author(s):  
Herison Victor Lima Muniz ◽  
Antonio Fialho da Silva Neto ◽  
Luiz Alfredo Torres Sales ◽  
Thalita Rodrigues Soares ◽  
Matheus Silva Alves
Keyword(s):  
Mycobacterium Tuberculosis ◽  
In Silico ◽  
Tuberculosis Strain ◽  
Strain H37rv

Mycobacterium tuberculosis é uma bactéria agente causadora responsável por grande parte dos casos de tuberculose (TB), mostrando-se como um grande problema de saúde pública. A TB é uma doença infectocontagiosa que atinge principalmente os pulmões, além de outros órgãos como rins, intestinos e meninges. O objetivo deste estudo foi desenhar e analisar primers de M. tuberculosis strain H37Rv da região 16S do rDNA para fins de diagnóstico molecular da tuberculose pela Reação em Cadeia da Polimerase (PCR). Foi utilizado a plataforma NCBI para obter a sequência completa do gene específico de M. tuberculosis, em seguida, cinco primers foram desenhados pela ferramenta BLAST do NCBI e analisados pela IDT integrated DNA Technologies, utilizando a ferramenta OligoAnalyzer. A partir da análise e desenho in silico dos cinco primers, foi observado que todos eles estavam dentro dos parâmetros estabelecidos. Sendo assim, os primers em teoria são eficientes para amplificar pares de bases e ser útil para o diagnóstico da tuberculose.

scholarly journals Black Cumin (Nigella sativa) Against Mycobacterium tuberculosis Strain H37RV And MDR-TB

Elkawnie ◽  
2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Mashuri Masri ◽  
Cut Muthiadin ◽  
Masita Masita ◽  
Tri Cahyanto ◽  
Lianah Lianah ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Multidrug Resistance ◽  
Microscopic Observation ◽  
Nigella Sativa ◽  
Drug Susceptibility ◽  
Tuberculosis Strain ◽  
Black Cumin ◽  
Mdr Tb ◽  
Susceptibility Assay ◽  
Strain H37rv

Abstract: Tuberculosis (TB) is a contagious infectious disease caused by Mycobacterium tuberculosis. 10 million people suffer from TB Every year. Although TB is a preventable and treatable disease, 1.5 million people die every year due to TB. Alternative treatments continue to be pursued, and treatment with the latest TB drugs that are continuously being encouraged. Black cumin (Nigella sativa) seed contains essential oils with active compounds such as thymohydroquinone, Oleoresins, flavonoids, alkaloids, saponins, tannins, and terpenoids that act as antibacterial drugs. This study aims to determine the sensitivity of  N. sativa seed extract in inhibiting the growth of  M. tuberculosis strain H37RV and MDR-TB (Multidrug Resistance-TB). This research using Microscopic-Observation and Drug-Susceptibility Assay (MODS) method. Extraction of N. sativa was carried out by the maceration method using 70% methanol as a solvent. The results showed that the M. tuberculosis strain H37RV and MDR-TB were sensitive to N. sativa extract at concentrations of 5 and 10% but resistant to N. sativa extract at concentrations of 1 and 3%.Abstrak: Tuberkulosis (TB) adalah penyakit menular yang disebabkan oleh Bakteri Mycobacterium tuberculosis. Penyakit ini menimbulkan dampak kematian yang cukup mengkhawatirkan.  Penyakit tersebut dapat dicegah dan diobati. Salah satu sumber pengobatannya menggunakan biji jintan hitam (Nigella sativa) yang mengandung minyak atsiri dengan senyawa aktif seperti timohidrokuinon, oleoresin, flavonoid, alkaloid, saponin, tanin, dan terpenoid yang berfungsi sebagai obat antibakteri. Penelitian ini bertujuan untuk mengetahui sensitivitas ekstrak biji N. sativa dalam menghambat pertumbuhan M. tuberculosis strain H37RV and MDR-TB (Multidrug-Resistance-TB). Penelitian ini menggunakan metode Microscopic-Observation and Drug-Susceptibility Assay (MODS). Ekstraksi N. sativa dilakukan dengan metode maserasi menggunakan pelarut metanol 70%. Hasil yang diperoleh menunjukkan bahwa bakteri M. tuberculosis strain H37RV dan TB-MDR, kedua  strain tsb sensitif terhadap ekstrak N. sativa konsentrasi 5 dan 10%,  tetapi resisten terhadap  ekstrak N. sativa konsentrasi 1 dan 3%.

scholarly journals Comparison of the proteome of Mycobacterium tuberculosis strain H37Rv with clinical isolate CDC 1551

Microbiology ◽  
2000 ◽  
Vol 146 (12) ◽  
pp. 3205-3216 ◽  
Author(s):  
Joanna C. Betts ◽  
Paul Dodson ◽  
Selwyn Quan ◽  
Alan P. Lewis ◽  
Pam J. Thomas ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Clinical Isolate ◽  
Tuberculosis Strain ◽  
Strain H37rv ◽  
Mycobacterium Tuberculosis Strain

scholarly journals Polisakarida Krestin dari Jamur Coriolus versicolor terhadap hitung Jenis Leukosit Mencit yang diinfeksi Mycobacterium tuberculosis

2017 ◽  
Vol 1 (2) ◽  
pp. 16-30
Author(s):  
Risa Purnamasari
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mus Musculus ◽  
Coriolus Versicolor ◽  
Tuberculosis Strain ◽  
Strain H37rv ◽  
Mycobacterium Tuberculosis Strain

Penelitian ini bertujuan untuk mengetahui peran polisakarida krestin (PSK) dengan waktu pemberian yang berbeda terhadap hitung jenis leukosit mencit yang diinfeksi Mycobacterium tuberculosis. Penelitian ini menggunakan 30 ekor mencit betina dewasa jenis Mus musculus strain BALB/C, berumur 8-10 minggu, berat badan berkisar 25-30 g. Polisakarida krestin (PSK) diisolasi dari Coriolus versicolor yang diperoleh dari alam. Infeksi menggunakan Mycobacterium tuberculosis strain H37Rv (ATCC 27294 T). Hewan percobaan dikelompokkan menjadi 6 kelompok sebagai berikut: kelompok I, hanya diberi akuades; kelompok II, hanya pemberian PSK; kelompok III, hanya dengan infeksi Mycobacterium tuberculosis; kelompok IV, pemberian PSK sebelum infeksi Mycobacterium tuberculosis; kelompok V, pemberian PSK sesudah infeksi Mycobacterium tuberculosis; kelompok VI, pemberian PSK sebelum dan sesudah infeksi Mycobacterium tuberculosis. Pemberian PSK dilakukan selama 7 hari berturut-turut melalui gavage. Infeksi Mycobacterium tuberculosis dilakukan sebanyak 2 kali dengan selang waktu 1 minggu melalui intraperitoneal. Hitung jenis leukosit dilakukan dengan mengelompokan masing-masing jenis leukosit dalam 100 sel leukosit pada apusan darah, dan data hasil pengamatan dianalisis dengan uji Kruskal-Wallis, kemudian untuk mengetahui signifikansi dilanjutkan dengan uji Mann-Whitney. Secara keseluruhan penelitian menunjukkan presentase jenis leukosit dengan jumlah tertinggi adalah neutrofil. Pada kelompok VI presentase monosit dan neutrofil meningkat melebihi normal, sedangkan presentase limfosit menurun, dan presentase basofil dan eosinofil tidak mengalami perubahan. Kesimpulan penelitian ini adalah PSK meningkatkan jumlah leuksosit mencit jenis neutrofil dan monosit pada waktu sebelum dan sesudah infeksi Mycobacterium tuberculosis

Cloning, Overexpression, and Purification of PhoR CytoplasmicDomain Protein from Mycobacterium tuberculosis strain H37Rv

2014 ◽  
Vol 8 (4) ◽  
pp. 141-146
Author(s):  
OKTIRA ROKA AJI ◽  
◽  
DYSHELLY NURKARTIKA PASCAPURNAMA ◽  
FENRYCO PRATAMA ◽  
IHSANAWATI IHSANAWATI ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Tuberculosis Strain ◽  
Strain H37rv ◽  
Mycobacterium Tuberculosis Strain

scholarly journals Aktivitas Anti Mycobacterium tuberculosis Strain H37Rv dan MDR (Multi Drug Resistant) dari Ekstrak Rimpang Curcuma mangga Val.

2020 ◽  
Vol 6 (1) ◽  
pp. 70-76
Author(s):  
Ritha Pratiwi ◽  
Gemini Alam ◽  
Herlina Rante ◽  
Nasrum Massi
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Microscopic Observation ◽  
Methanol Extract ◽  
Hexane Extract ◽  
Tuberculosis Strain ◽  
Tuberculosis Activity ◽  
Strain H37rv ◽  
Mycobacterium Tuberculosis Strain ◽  
Made In

TB is an infectious disease caused by the bacterium Mycobacterium tuberculosis. Empirically the plants that have long been used in medicine are Curcuma mangga  rhizome, which shows pharmacological activity as an anti Mycobacterium tuberculosis drug. The objective of the research is to determine the ability of hexane and methanol extracts of Curcuma mangga in inhibiting the growth of Mycobacterium tuberculosis Strain H37Rv and MDR. The extraction of Curcuma mangga rhizome was conducted by maceration methode using hexane and methanol as the solvents, respectively.  Both of extracts were made in 1000 dan 500 ppm concentration, then tested for anti Mycobacterium tuberculosis activity using MODS (Microscopic Observation Drug Suspectibility) method. Determination of anti M.tuberculosis activity was based on microscopic observation on Mycobacterium tuberculosis colony in cord formation. The results showed that hexane and methanol extract in 1000 ppm concentration inhibited the growth of Mycobacterium tuberculosis Strain H37Rv dan MDR. However in 500 ppm only n-hexane  extract showing the inhibiton activity. In conclusion, n-hexane extract have the ability to inhibit the growth of Mycobacterium tuberculosis Strain H37Rv dan MDR. 

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