scholarly journals In vitro Barnardia japonica (Thunb.) Schult. et Schult. fil. micropropagation by direct regeneration

2021 ◽  
Author(s):  
Anastasiya S. Pianova ◽  
◽  
Aleksey V. Salokhin ◽  
Tat’yana E. Lonchakova ◽  
Yuri E. Sabutski ◽  
...  
Keyword(s):  
Natural Habitat ◽  
Direct Regeneration ◽  
High Concentration ◽  
Regeneration Potential ◽  
In Vitro Micropropagation ◽  
Naphthylacetic Acid ◽  
Far Eastern ◽  
First Time ◽  
Eastern Species

In vitro micropropagation of rare Far Eastern species Barnardia japonica by the direct regeneration method was studied for the first time. The effect of different concentrations of 6-benzylaminopurine and kinetin in combination with auxins on the regeneration potential of young seedlings using Murashige and Skoog media was estimated. It was found that the largest number of new plants (61 new shoots on average) is formed when explants are cultivated on the medium with a high concentration of kinetin (10 mg/L) and a minimum of α-naphthylacetic acid (0.1 mg/L). The resulting plants are rooted easily on a hormone-free medium of the same mineral composition. The least effective for the mass production of plants by direct regeneration was a medium with a combination of 2 mg/L kinetin and 1 mg/L α-naphthylacetic acid. The developed approach can be applied for the effective propagation of Barnardia japonica and returning the species to its natural habitat.

A revision of the subfamily Araeopteroninae Fibiger, 2005 in the Russian Far East and neighbouring countries with a description of four new species (Lepidoptera, Noctuidae)

Zootaxa ◽  
2008 ◽  
Vol 1891 (1) ◽  
pp. 39
Author(s):  
MICHAEL FIBIGER ◽  
VLADIMIR KONONENKO
Keyword(s):  
Russian Far East ◽  
Far East ◽  
Morphological Characters ◽  
Male And Female ◽  
Distribution Maps ◽  
Far Eastern ◽  
First Time ◽  
Lepidoptera Noctuidae ◽  
Eastern Species ◽  
The Russian Far East

The subfamily Araeopteroninae Fibiger, 2005 from the Russian Far East and neighbouring countries is revised. Six species occur in Russia, three of which are new: A. ussurica sp. n., A. makikoae sp. n. and A. patella sp. n.; A. fragmenta and A. nebulosa are reported for the first time for Russia. In South Korea five species occur, two of which are new: A. patella sp. n. and A. koreana sp. n. Three species, A. amoena, A. nebulosa, and A. fragmenta are reported for the first time from China. The formerly described Far Eastern species (A. amoena, A. nebulosa, A. fragmenta and A. flaccida) are redescribed based on morphological characters. Adultss of all species from northern East Asia treated in the article are illustrated in color with their male and female genitalia. Distribution maps of the species in the Russian Far East are presented, along with an alphabetic checklist of 36 described species.

scholarly journals P.235 Differences in Human, Pig, and Rat Spinal Cord Stem Cells in Response to Inflammatory and Regenerative Factors In Vitro

2021 ◽  
Vol 48 (s3) ◽  
pp. S88-S88
Author(s):  
A Galuta ◽  
A Yassin Kassab ◽  
D Ghinda ◽  
R Sandarage ◽  
J Kwan ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Inflammatory Factors ◽  
Direct Regeneration ◽  
Post Injury ◽  
Promising Therapeutic Approach ◽  
Cord Injury ◽  
Neural Stem Progenitor Cells ◽  
Regenerative Therapies ◽  
First Time

Background: While the use of neural stem/progenitor cells has been reported as a promising therapeutic approach for spinal cord injury, direct comparison of adult primary animal spinal cord NSPCs have not been compared to human NSPCs under the same conditions to characterize intrinsic differences between human/animal NSPC response to inflammatory/regenerative factors. Methods: To mimic post-injury inflammation, primary-derived NSPCs from adult humans, pigs, and rats were treated with pro-inflammatory factors.To direct regeneration, NSPCs were treated with retinoic acid, platelet-derived growth factor or bone morphogenic protein-(BMP4) to induce neurons, oligodendrocytes or astrocytes, respectively.Cultures were treated for 7 or 14 days and characterized by immunocytochemistry. Results: Pro-inflammatory factors promoted more astrogenesis in rat and pig NSPCs compared to human NSPCs and induced neuronal differentiation in human NSPCs. RA increased neurogenesis of human and rat NSPCs, PDGFα increased oligodendrocyte differentiation of rat NSPCs, and BMP4 increased astrogenesis of human and rat NSPCs Conclusions: For the first time, differences in response of human, pig and rat primary NSPCs to inflammatory and regenerative factors have been identified. Better understanding of these differences is essential to improving the successful translation of regenerative therapies to humans.

scholarly journals TDZ Plays Key Role in Shoot Regeneration from Different Explants of Picrorhiza kurroa: An Endangered Medicinal Herb of Western Himalayas

2019 ◽  
pp. 1-9 ◽  
Author(s):  
Vanita Patial ◽  
Amita Bhattacharya
Keyword(s):  
Natural Habitat ◽  
Basal Medium ◽  
In Vitro Rooting ◽  
Western Himalayas ◽  
Picrorhiza Kurroa ◽  
Regeneration Potential ◽  
Root Explants ◽  
Shoot Number ◽  
Endangered Medicinal Herb

Picrorhiza kurroa plants were collected from its natural habitat. In vitro plants were raised from the leaves of high yielding collection screened in an earlier study. Leaves, roots and internodal segments were cultured for 15 days. The effect of thidiazuron (1-phenyl-3-(1, 2, 3- thiadiazol-5-yl) urea; TDZ) pretreatment for 15 days on regeneration potential of different explants viz. leaves, roots and internodes of Picrorhiza kurroa was studied. Regeneration potential varied significantly with the type of explant. Regeneration response of 100% with 46.25 shoots per explant was obtained in leaf segments of 2.0 cm length pretreated with 0.5 µM TDZ for 15 days and then transferred to 2.32 µM kinetin (Kn) containing MS basal medium. In case of root explants maximum shoot number (17.12) was obtained on 0.5 µM TDZ pretreated for 15 days and then to 3.64 µM Kn. Maximum shoots per explants (12.33) were obtained in case of internodes pretreated with 0.5 µM TDZ for 15 days and transferred to 1.16 µM Kn. Regenerated shoots from different explants developed in vitro rooting on MS basal medium within 7-8 days. Conclusively, an efficient and repeatable protocol for rapid regeneration from different explants and in vitro rooting has been developed in P. kurroa which can be effectively used for its conservation.

scholarly journals A Study on the Quality and Identity of Brazilian Pampa Biome Honey: Evidences for Its Beneficial Effects against Oxidative Stress and Hyperglycemia

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
L. C. Cruz ◽  
J. E. S. Batista ◽  
A. P. P. Zemolin ◽  
M. E. M. Nunes ◽  
D. B. Lippert ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
High Concentration ◽  
Beneficial Effects ◽  
Pampa Biome ◽  
First Time

We characterized, for the first time, the quality and identity of Brazilian Pampa biome honey and its antioxidant propertiesin vitro(FRAP, DDPH and ABTS). The potential protective effect of honey against oxidative stress induced by iron (Fe) and paraquat, (PQ) in aDrosophila melanogastermodel (in vivo) was also tested. The results indicated that all honey samples tested showed antioxidant activityin vitro. Flies treated with honey showed increased lifespan and were protected against oxidative stress induced by Fe and PQ. Despite the high concentration of sugars in honey (approximately 70–80%), our results demonstrate a hypoglycemic-like effect of honey inDrosophila. Thus, this study demonstrates the high quality of Brazilian Pampa biome honey as well as its significant antioxidant activityin vitroandin vivo, pointing to the potential use of this natural product as an alternative in the therapy of oxidative stress-associated diseases.

scholarly journals Antimicrobial Potential of Vapour Phase Acetic Acid in Combination with Ethyl Alcohol against Salmonella Typhimurium Contaminated on Bird Eye Chili (Capsicum frutescens L.)

2018 ◽  
Vol 192 ◽  
pp. 03053
Author(s):  
Pasit Nichayapun ◽  
Theeraporn Khawsa-ard ◽  
Todsaphon Pimrat ◽  
Pokpong Lilitsajja ◽  
Racha Tepsorn
Keyword(s):  
Acetic Acid ◽  
Ethyl Alcohol ◽  
Bacterial Contamination ◽  
Food Poisoning ◽  
Vapour Phase ◽  
Capsicum Frutescens ◽  
High Concentration ◽  
First Time ◽  
Safe Approach

Bacterial contamination is the main cause of food poisoning. The currently decontamination methods arise to find novel, effective and safe approach. Acetic acid (AC) in both aqueous and vapour forms have been conducted on its effectiveness against several bacterial contaminations. The use in high concentration can cause strong smell and corrosives that have an effect on the qualities of food. This study aim to assess the potential of Mechanically Vapourized AC solution in combination with ethyl alcohol (EA) (MVA-E) on the reduction of Salmonella enterica serotype Typhimurium ATCC 13311 contaminated on both microbiological media and on fresh Bird Eye Chili (Capsicum frutescens L.). In vitro surface inhibitions of S. Typhimurium at low population, ca. 1.0 CFU/cm2, and high population, ca. 10.0 CFU/cm2 were examined on Tryptic Soya Agar (TSA). MVA-E at the concentration of 10:95 (AC:EA) presented the absolutely inhibited S. Typhimurium within 20 min at 27±2°C. At the concentration of 10:75 (AC:EA), the absolutely inactivated S. Typhimurium was observed within 10 min at 50±2°C. For the evaluation of antimicrobial activity of MVA-E over time, the results indicated that ca. 8.00 Log10 reductions were observed within 20, 25 and 25 min at the concentration of AC:EA ratio as 10:95, 10:75, and 10:45, respectively at 27±2°C. The effectiveness of MVA-E increased when the temperature of MVA-E process increased. The reduction of S. Typhimurium contaminated on fresh Bird Eye Chili by MVA-E was also determined at 4°C, 27±2°C and 50°C. The efficiency of MVA-E on the reduction of S. Typhimurium depended on the concentration of EA in MVA-E process, the fumigation time and also the temperature. To the best of knowledge this is the first time a combination of AC and EA in vapour phase has been tested as a preservative method prevent microorganism proliferation.

Species of the genus Harpocera Curt. from the Russian Far East (Heteroptera: Miridae)

2006 ◽  
Vol 15 (1) ◽  
pp. 83-85
Author(s):  
N.N. Vinokurov
Keyword(s):  
Russian Far East ◽  
Far East ◽  
Kuril Islands ◽  
Far East Of Russia ◽  
The Far East ◽  
The Kuril Islands ◽  
Far Eastern ◽  
First Time ◽  
Eastern Species ◽  
The Russian Far East

Three species of the Palaearctic genus Harpocera Curt, are distributed in the Far East of Russia H. koreana Jos. is recorded from Russia for the first time, the hitherto unknown males of H. choii Jos. and H. koreana Jos. are described and compared with H. orientalis Kerzh. from the Kuril Islands. A key to the three Far Eastern species is given.

Non-biting midges of the genus Paracladopelma Harnisch (Diptera: Chironomidae) from the Russian Far East

Zootaxa ◽  
2006 ◽  
Vol 1134 (1) ◽  
pp. 29 ◽  
Author(s):  
OKSANA V. ZORINA
Keyword(s):  
New Species ◽  
Russian Far East ◽  
Far East ◽  
Junior Synonym ◽  
Biting Midges ◽  
Male Morphology ◽  
Far Eastern ◽  
First Time ◽  
Eastern Species ◽  
The Russian Far East

The Far Eastern species of the genus Paracladopelma Harnisch, 1923 are revised. Four new species of Paracladopelma are described: P. augustus, P. globosum, P. pseudocamptolabis, and P. urkanensis. Seven species P. doris (Townes), P. furudoprimum Sasa, P. laminatum (Kieffer), P. nais (Townes), P. nereis (Townes), P. nigritulum (Goetghebuer), and P. undine (Townes) are redescribed on the basis of male morphology. Six species are recorded from Russia for the first time. Paracladopelma simantodeea Sasa et al. 1998 is presented as a junior synonym of Paracladopelma furudoprimum Sasa & Arakawa. A key for males of 12 Far Eastern species of Paracladopelma is given.

Somatic Embryogenesis and In vitro Plant Regeneration in Vigna trilobata (L.) Verd. - A Potential Pasture Legume

1970 ◽  
Vol 19 (1) ◽  
pp. 89-99
Author(s):  
K. Choudhary ◽  
M. Singh ◽  
M. S. Rathore ◽  
N. S. Shekhawat
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Long Term Study ◽  
Continuous Application ◽  
First Time ◽  
Pasture Legume

This long term study demonstrates for the first time that it is possible to propagate embryogenic Vigna trilobata and to subsequently initiate the differentiation of embryos into complete plantlets. Initiation of callus was possible on 2,4-D. Somatic embryos differentiated on modified MS basal nutrient medium with 1.0 mg/l  of 2,4-D and 0.5 mg/l  of Kn. Sustained cell division resulted in globular and heart shape stages of somatic embryos. Transfer of embryos on to a fresh modified MS basal medium with 0.5 mg/l of Kn and 0.5 mg/l of GA3 helped them to attain maturation and germination. However, the propagation of cells, as well as the differentiation of embryos, were inhibited by a continuous application of these growth regulators. For this reason, a long period on medium lacking these growth regulators was necessary before the differentiation of embryos occurred again. The consequences for improving the propagation of embryogenic cultures in Vigna species are discussed. Key words: Pasture  legume, Vigna trilobata, Globular, Heart shape, somatic embryogenesis D.O.I. 10.3329/ptcb.v19i1.4990 Plant Tissue Cult. & Biotech. 19(1): 89-99, 2009 (June)

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