scholarly journals TDZ Plays Key Role in Shoot Regeneration from Different Explants of Picrorhiza kurroa: An Endangered Medicinal Herb of Western Himalayas

2019 ◽  
pp. 1-9 ◽  
Author(s):  
Vanita Patial ◽  
Amita Bhattacharya
Keyword(s):  
Natural Habitat ◽  
Basal Medium ◽  
In Vitro Rooting ◽  
Western Himalayas ◽  
Picrorhiza Kurroa ◽  
Regeneration Potential ◽  
Root Explants ◽  
Shoot Number ◽  
Endangered Medicinal Herb

Picrorhiza kurroa plants were collected from its natural habitat. In vitro plants were raised from the leaves of high yielding collection screened in an earlier study. Leaves, roots and internodal segments were cultured for 15 days. The effect of thidiazuron (1-phenyl-3-(1, 2, 3- thiadiazol-5-yl) urea; TDZ) pretreatment for 15 days on regeneration potential of different explants viz. leaves, roots and internodes of Picrorhiza kurroa was studied. Regeneration potential varied significantly with the type of explant. Regeneration response of 100% with 46.25 shoots per explant was obtained in leaf segments of 2.0 cm length pretreated with 0.5 µM TDZ for 15 days and then transferred to 2.32 µM kinetin (Kn) containing MS basal medium. In case of root explants maximum shoot number (17.12) was obtained on 0.5 µM TDZ pretreated for 15 days and then to 3.64 µM Kn. Maximum shoots per explants (12.33) were obtained in case of internodes pretreated with 0.5 µM TDZ for 15 days and transferred to 1.16 µM Kn. Regenerated shoots from different explants developed in vitro rooting on MS basal medium within 7-8 days. Conclusively, an efficient and repeatable protocol for rapid regeneration from different explants and in vitro rooting has been developed in P. kurroa which can be effectively used for its conservation.

scholarly journals Media composition affects seed dormancy, apical dominance and phenolic profile of Knautia sarajevensis (Dipsacaceae), Bosnian endemic

2018 ◽  
Vol 77 (1) ◽  
pp. 70-79 ◽  
Author(s):  
Erna Karalija ◽  
Sanja Ćavar Zeljković ◽  
Petr Tarkowski ◽  
Edina Muratović ◽  
Adisa Parić
Keyword(s):  
Apical Dominance ◽  
Germination Rate ◽  
Natural Habitat ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Mineral Nutrient ◽  
Total Phenolic ◽  
Endemic Plant ◽  
High Concentrations

AbstractKnautia sarajevensisis an endemic plant of the Dinaric Alps and is mainly distributed on Bosnian Mountains. Due to the quite large flower heads and easy maintenance, this plant has a potential use as a substitute ornamental plant forK. arvensisin perennial beds. The current study evaluated the germination process in different treatments in an attempt to suppress dormancy and increase germination rate, and to develop a successful protocol for micropropagation. An over 60% germination rate was achieved through cultivation of seeds on MS basal medium with reduced mineral nutrient composition and the absence of sucrose. On the other hand, a below 10% germination rate was achieved with untreated seeds. Suppression of apical dominance was achieved through application of high concentrations of kinetin, apical shoot decapitation or cultivation of shoots in liquid media. Overall, liquid cultures were more successful as a micropropagation system for this plant. Shoots spontaneously developed roots on multiplication treatments and were successfully acclimatized. Moreover, phenolic compound profile was analysed in the light of the possible medicinal potential of this plant. Variable amounts of total phenolic compounds as well as individual phenolics were recorded, according to treatment and solidification of media. An increase in rosmarinic acid content was reported for kinetin treatments and acclimatized plants comparing to mother plants in natural habitat. The present study shows that choice of cytokinin concentration, explant type as well as culture type influences not only shoot proliferation and apical dominance suppression but alsoin vitroproduction of phenolics.

scholarly journals Propagation for the Conservation of Pityopsis ruthii, an Endangered Species from the Southeastern United States

HortScience ◽  
2014 ◽  
Vol 49 (2) ◽  
pp. 194-200 ◽  
Author(s):  
Phillip A. Wadl ◽  
Timothy A. Rinehart ◽  
Adam J. Dattilo ◽  
Mark Pistrang ◽  
Lisa M. Vito ◽  
...  
Keyword(s):  
Endangered Species ◽  
Natural Habitat ◽  
Basal Medium ◽  
Single Individual ◽  
In Vitro Multiplication ◽  
Large Numbers ◽  
Lateral Shoots ◽  
Genetic Swamping ◽  
Germination Techniques

Pityopsis ruthii is an endangered species endemic to the Hiwassee and Ocoee Rivers in Tennessee. As part of a recovery effort focused on P. ruthii, vegetative propagation and in vitro multiplication and seed germination techniques were developed. Plants were vegetatively propagated using greenhouse stock plants and wild-collected stems. Rooting occurred with and without auxin treatments but was greatest when 0.1% indole-3-butyric acid (IBA) talc was applied to the vegetative cuttings; rooting was lowest when flowering stems were used. Pro-Mix BX substrate provided the most consistent rooting. In vitro multiplication was accomplished by the removal of lateral shoots from in vitro-grown plants that were rooted on Murashige and Skoog (MS0) basal medium with 270 clones produced from a single individual after 4 months. Nineteen clones were transplanted and secured with bonded fiber matrix into their natural habitat and 14 survived for 1 year. To avoid genetic swamping of native populations with the introduction of large numbers of genetically identical individuals through clonal propagation, seed-based propagation efforts were explored. Open-pollinated seeds were collected, disinfested and germinated, and seedlings established on MS medium. Seeds were submersed in 70% ethanol for 1 minute and briefly flamed. Seeds were surface-sterilized in a range [10% to 50% (v/v)] Clorox® bleach solutions with vigorous shaking for 20 minutes, rinsed three times in sterile water, and germinated on MS0. Removal of pappus from seeds was required for successful disinfestations, but the bleach concentration was not critical. Successful propagation is a step toward the conservation and recovery of P. ruthii and should allow future reintroduction projects.

scholarly journals In vitro Multiple Shoot Regeneration from Petunia hybrida

2019 ◽  
Vol 7 (10) ◽  
pp. 1554
Author(s):  
Rebaz Rasul Habas ◽  
Musa Turker ◽  
Fethi Ahmet Ozdemir
Keyword(s):  
Petunia Hybrida ◽  
Survival Rates ◽  
Basal Medium ◽  
Multiple Shoot ◽  
Shoot Length ◽  
Peat Moss ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Shoot Number

An efficient plant regeneration protocol was developed from in vitro germinated seeds of Petunia hybrida an ornamentally important plant in the family Solanaceae. Shoot tip and node explants of Petunia hybrida were cultured on MS basal medium supplemented with different concentrations and combinations of Benzyl amino purine (BAP), 1-Naphthaleneacetic acid (NAA), Indole-3-butyric acid (IBA) and Gibberellic acid (GA3). The highest shoot length was obtained from MS medium supplemented with 1 mg/l BAP + 1 mg/l NAA. The highest shoot number (3 shoots/explant) were obtained from MS medium supplemented with 0.6 mg/l BAP + 0.5 mg/l IBA. The isolated shoots were transferred to MS basal medium supplemented with different concentrations of GA3 ranging from 0.05, 0.2, 0.5 and 1 mg/l for shoot elongation. The highest shoot length (5.75 cm) was recorded from the MS medium supplemented with 0.2 mg/l GA3 +0.2 mg/l BAP. Rooting of regenerated shoots were achieved on MS medium supplemented with 0.1-1 mg/1 IBA and NAA. The regenerated shoots with well developed roots were successfully acclimatized and established in pots containing sterilized peat moss and grown under laboratory conditions with 70% survival rates.

Callus and cell suspension culture of Chelidonium majus L

2014 ◽  
Vol 11 (2) ◽  
pp. 150-154
Author(s):  
S Otgonpurev ◽  
Kh Altantsetseg ◽  
N Tsevegsuren
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Basal Medium ◽  
Ms Medium ◽  
Chelidonium Majus ◽  
Root Explants ◽  
Root Callus ◽  
Murashige Skoog

Chelidonium majus L has long history as a being useful for the treatment of many diseases in Asian and European countries. Aim of this study is to cultivate callus and cell suspension culture in vitro using plant phytohormones. The proliferative capacity was tested on shoot and root explants, cultivated on Murashige-Skoog basal medium testing two auxins: 2,4-diclorphenoxiacetic acid (2.4D) and napheteline acetic acid in combination with cytokinine: kinnetine (K). Calluses were developed on MS medium with 0.5 mg/l Kin, 0.5 mg/l IAA from root explants, as well when added with 0.5 mg/l NAA and 0.5 mg/l Kin from shoot explants. More biomass of cell suspension culture of shoot and root callus was accumulated on MS medium added with 0.1mg/l Kin. DOI: http://dx.doi.org/10.5564/mjas.v11i2.238 Mongolian Journal of Agricultural Sciences Vol.11(2) 2013 pp.150-154

scholarly journals In vitro Barnardia japonica (Thunb.) Schult. et Schult. fil. micropropagation by direct regeneration

2021 ◽  
Author(s):  
Anastasiya S. Pianova ◽  
◽  
Aleksey V. Salokhin ◽  
Tat’yana E. Lonchakova ◽  
Yuri E. Sabutski ◽  
...  
Keyword(s):  
Natural Habitat ◽  
Direct Regeneration ◽  
High Concentration ◽  
Regeneration Potential ◽  
In Vitro Micropropagation ◽  
Naphthylacetic Acid ◽  
Far Eastern ◽  
First Time ◽  
Eastern Species

In vitro micropropagation of rare Far Eastern species Barnardia japonica by the direct regeneration method was studied for the first time. The effect of different concentrations of 6-benzylaminopurine and kinetin in combination with auxins on the regeneration potential of young seedlings using Murashige and Skoog media was estimated. It was found that the largest number of new plants (61 new shoots on average) is formed when explants are cultivated on the medium with a high concentration of kinetin (10 mg/L) and a minimum of α-naphthylacetic acid (0.1 mg/L). The resulting plants are rooted easily on a hormone-free medium of the same mineral composition. The least effective for the mass production of plants by direct regeneration was a medium with a combination of 2 mg/L kinetin and 1 mg/L α-naphthylacetic acid. The developed approach can be applied for the effective propagation of Barnardia japonica and returning the species to its natural habitat.

scholarly journals The effect of different cytokinins on chlorophyll content and morphological features of in vitro Nidularium ’Kertész Jubileum’

2015 ◽  
Vol 21 (1-2) ◽  
Author(s):  
M. Ördögh
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Basal Medium ◽  
The Other ◽  
Naphthaleneacetic Acid ◽  
Statistical Software ◽  
In Vitro Multiplication ◽  
Shoot Number ◽  
Definite Correlation

During in vitro multiplication of Nidularium ‘Kertész Jubileum’, 20 g/l sucrose, 5 g/l agar, 100 mg/l inositol, and different concentrations of benzyladenine (BA), benzyladenine-riboside (BAR), kinetin (KIN), meta-topolin (mT) were added to the MKC (Knudson, 1946) basal medium. Furthermore, 0.1 mg/l naphthaleneacetic acid was used to every medium. Number of shoots, length of leaves, number and length of roots, chlorophyll (a+b) content were examined and evaluated with Ropstat statistical software. As compared to the other cytokinin, significantly most shoots were obtained in the case of applying BA. Increasing of BA-concentration (as far as 2 mg/l) enhanced shoot number (from 10.92 to 19.26) but 4 mg/l BA resulted only 6.63 shoot. The less efficient cytokinin was KIN, in most cases no more than about 2 shoot was achieved. Regarding the length of leaves, the higher level of BA effected averagely the shorter leaves (from 24,46 to 7.31 mm). KIN effected significantly the longest leaves (43.4-61.29) in inverse proportion to the concentration. The same cytokinin resulted the most (and the longest) roots with the highest rooting percentages, but more KIN decreased the number and length of roots (from 7.95 to 4.4 and from 38.49 to 22.73 mm). There were no definite correlation between cytokinin concentration and chlorophyll (a+b) content, but the highest doses resulted decreasing (except of meta-topolin which leads to the lowest values). Summarizing, BAR effected the highest contents (mostly more than 1400 μg/g), particularly in the case of 1 mg/l (1807.3 μg/g).

scholarly journals EFFECT OF SUCROSE AND MEDIA STRENGTH ON IN VITRO MULTIPLICATION IN SWERTIA CHIRATA BUCH.-HAM EX WALL: AN ENDANGERED MEDICINAL HERB

2018 ◽  
Vol 10 (11) ◽  
pp. 40
Author(s):  
Manu Pant ◽  
Prabha Bisht ◽  
Manju P Gusain
Keyword(s):  
Shoot Multiplication ◽  
Axillary Shoot ◽  
Ms Medium ◽  
Carbohydrate Source ◽  
Windows Nt ◽  
Swertia Chirata ◽  
Shoot Number ◽  
Endangered Medicinal Herb ◽  
Experimental Findings

Objective: The present study was performed to investigate the role of varying concentrations of carbohydrate source and strengths of nutrient medium in growth and development of in vitro shoots of Swertia chirata-an endangered medicinal plant.Methods: MS medium supplemented with 6-Benzylaminopurine (4.44 µM), Indole-3 acetic acid (2.85 µM) and Adenine sulphate (271.45 µM) was used to test the efficiency of of sucrose at concentrations of 1-5% and of media strength varying from full to one-fourth. The data were analysed using analysis of variance (ANOVA) of Completely Randomized Design (CRD) in GenStat 5 Edition 3.2 for PC/Windows NT (Copyright 1995, Lawes Agricultural Trust (Rothamsted Experimental Station))Results: Observations on axillary shoot multiplication indicated that sucrose at a concentration of 3% and MS medium in its full strength proved to be most optimal for in vitro culture multiplication. On this medium combination mean number of 11.80 shoots (after 4 w) and 18.50 shoots (after 8 w) could be obtained On sucrose free medium the shoots exhibited necrosis while at lower concentrations of 1-2% sucrose, the shoots developed were thin and unsuitable for further growth in vitro. At higher levels of sucrose in the medium, the shoots became thick and stunted. Similarly, reduction in medium strength resulted in a decline in shoot number and shoot length to an average of 6.50 shoots (1.33 cm mean length) on half strength medium and 5.60 shoots (0.88 cm mean length) on one-fourth strength; as observed after 4 w.Conclusion: The experimental findings suggest that any decline from the standard had a significant effect on the number, size and overall health of shoots developed in vitro. The conditions so standardized augment the production of healthy shoots that shall aid in subsequent rooting and survival after transplantation of tissue-culture raised plantlets.

scholarly journals Micropropagation of `Norton' Winegrape

2001 ◽  
Vol 11 (2) ◽  
pp. 206-208 ◽  
Author(s):  
M.A. Norton ◽  
R.M. Skirvin
Keyword(s):  
Basal Medium ◽  
Shoot Multiplication ◽  
Shoot Length ◽  
In Vitro Rooting ◽  
Axillary Bud ◽  
Naphthaleneacetic Acid ◽  
Single Node ◽  
Vitis Aestivalis ◽  
Four Levels

A method has been developed for micropropagation of the difficult-to-root winegrape cultivar `Norton' (Vitis aestivalis). Plants were established in vitro from axillary bud cuttings of field-grown plants. Four levels of 6-benzylaminopurine (BA) and three levels of naphthaleneacetic acid (NAA) were tested in a factorial arrangement for their effectiveness in promoting multiplication of shoots from single-node explants. Three levels of NAA and two concentrations of Murashige and Skoog (MS) basal medium were tested for their effectiveness in promoting rooting of shoot tips. The greatest number of shoots per axillary bud in combination with the greatest shoot length were produced with 4 μmol·L-1 [0.90 mg·L-1 (ppm)] BA. NAA had no effect on shoot multiplication. NAA was not required for in vitro rooting. All rooted plants survived the transition to soil.

scholarly journals Development of An Efficient Protocol for Rapid Propagation of Curculigo orchioides Using Leaf Explant Culture

2021 ◽  
Vol 14 (4) ◽  
pp. 1514-1519
Author(s):  
Tran Thi Linh Giang
Keyword(s):  
Leaf Explants ◽  
Basal Medium ◽  
Culture Method ◽  
Explant Culture ◽  
Regeneration Rate ◽  
Curculigo Orchioides ◽  
Rapid Propagation ◽  
Best Response ◽  
Shoot Number

Curculigo orchioides is one of the most common medicinal plants used by diverse cultures and tribal groups. The roots of the plant are used medicinally in Asian countries. Curculigo orchioides have the ability to regenerate through seeds and tubers, but the regeneration rate is low. Plant tissue culture method was believed to have potential for rapid multiplication of this medicinal plant. An efficient protocol for rapid propagation of Curculigo orchioides, of the family Amaryllidaceae, was developed using leaf explants culture. The leaf explants (1 cm x 1 cm squares) cultured on Murashige and Skoog (MS) basal medium were supplemented with various concentrations and combinations of auxins and cytokinins with temperature 25 ± 2°C, relative humidity 85-90% and photoperiod of 12 hours light (2000-3000 lux). Callus induction was obtained within 4 weeks, 2,4-D at 3 mg/l formed profuse callus and the degree was found to be the highest (+++) among all the treatments. The best response to shoot induction, with maximum shoot number 5.33 (mean number of shoots per explant) was obtained using 1.0 mg/l 6-benzyl aminopurine (BAP) in combination with 1.0 mg/l Kinetin. In vitro shoots were induced for rooting on 0.5 mg/l of NAA supplemented medium. In order for seedlings propagated in vitro to adapt to natural conditions, plants were growned on a substrate coir: husk ash: sand (with the ratio of 0.5: 0.5: 1) in a greenhouse (humidity: 70%, temperature: 28-300C) gave 88.33% survival rate after 8 weeks of culture. With the results received, this is an effective approach to propagating Curculigo orchioides.

scholarly journals Aspectos anatômicos da cultura in vitro da Araucaria angustifolia. II. 0 enraizarnento dos brotos axilares

1993 ◽  
Vol 22 ◽  
Author(s):  
Cecília Iritani ◽  
Flávio Zanette ◽  
Jovita Cislinski
Keyword(s):  
Butyric Acid ◽  
Basal Medium ◽  
Mineral Nutrients ◽  
In Vitro Rooting ◽  
Araucaria Angustifolia ◽  
Root Primordia ◽  
Friable Callus ◽  
Half Strength

Foram estudados os aspéctos anatômicos da estrutura do calo do processo do enraizamento dos brotos axilares da Araucaria angustifolia. Estes brotos, em meio básico de Murashigue & Skoog, com a concentração dos nutrientes minerais reduzida à metado 1-2 mg de ácido indol-3-butírico. formam calo rizogênico somente nas regiões muito próximas da base. 0 excedente é do tipo friável, provavelmente dificultando a iniciação radicial. Entretanto. as raízes formadas são morfo e fisiologicamente funcionais. Abstract Anatomical aspects of the callus and the in vitro rooting process on axiliary shoots of Araucaria angustifoia were performed. These shoots cultivated in Murashigue & Skoog’s basal medium at the half strength of mineral nutrients and 1-2 mg of indol-3-butyric acid, showed firmly stablished and rhizogenic callus only at the proximal sites of its bases. The excedent were friable callus, probably affecting the root primordia initiation. However, developed roots are phisiologicalty and morphologically functional. Abstract Les aspects anatomiques de la structure du cal et de la rhizogénese in vitro de pousses axillaires d’Araucaria angustifolia ont été étudiés. Cés pousses cultivées sur le milieu de Murashigue & Skoog dans une concentration des minéraux réduit a 1/2, plus 1-2 mg de L’acid 3- indolybutirique, forment des cals compacts rhizogénes seulement a la proximité de leurs bases. L’excedant étaient du tipe friable, possiblement impechent l‘initiation des racines. Les racines formées sur les cals compacts, sont morphologique et physiologiquement functionelles.

Sign in / Sign up

Export Citation Format

Share Document