scholarly journals Chlorophyll fluorescence and other physiological parameters as indicators of waterlogging and shadow stress in lulo (Solanum quitoense var. septentrionale) seedlings

2019 ◽  
Vol 13 (3) ◽  
pp. 325-335
Author(s):  
Alefsi David Sánchez-Reinoso ◽  
Yulieth Jiménez-Pulido ◽  
Jean Paul Martínez-Pérez ◽  
Carlos Salvador Pinilla ◽  
Gerhard Fischer
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Chlorophyll A Fluorescence ◽  
Photochemical Quenching ◽  
Low Light ◽  
Ps Ii ◽  
Randomized Design ◽  
Relative Water ◽  
Four Levels ◽  
Photochemical Quantum Yield

Climate change has resulted in an increasing frequency of the phenomenon “La Niña,” generating prolonged periods of waterlogging and low light. The objective of the present study was to evaluate the effects of two abiotic stresses: shading (65%) and waterlogging, and their interaction on fluorescence parameters of chlorophyll a in lulo (Solanum quitoense var. septentrionale) seedlings. A completely randomized design with a factorial arrangement was implemented. The first factor consisted of two levels of light (with and without shading). The second factor were four levels of duration of the waterlogging period (0, 3, 6 and 9 days), for a total of 8 treatments with three replicates. The response variables were recorded at 6, 12 and 18 days after the application of the waterlogging treatments began. Measurements of relative water content (RWC), electrolyte leakage, chlorophyll content and chlorophyll a fluorescence were recorded. The lulo plants appeared to be more susceptible to waterlogging than to shading, with a lower RWC when waterlogged 6 and 9 days, presenting damage at the level of photosystem II from day 3, causing a decrease in the chlorophyll content. The plants flooded under shading had a greater tolerance to this factor than those cultivated in full light. The techniques of quantification of the chlorophyll a fluorescence, especially the maximum quantum efficiency of the PSII, the effective photochemical quantum yield of PS II and the photochemical quenching were useful tools that characterized the lulo seedlings under stress conditions.

scholarly journals Effect of Salicylic Acid Pre-Treatment after Long-Term Desiccation in the Moss Syntrichia ruralis (Hedw.) Web. and Mohr

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1097
Author(s):  
Ruchika ◽  
Zsolt Csintalan ◽  
Evelin Ramóna Péli
Keyword(s):  
Salicylic Acid ◽  
Seasonal Variation ◽  
Quantum Yield ◽  
Chlorophyll A Fluorescence ◽  
Photochemical Quenching ◽  
Ps Ii ◽  
Fluorescence Parameters ◽  
Non Photochemical Quenching ◽  
Photochemical Quantum Yield

The main objective of this research was to examine the effects of exogenous salicylic acid (SA) and to study the seasonal variation of the chlorophyll a fluorescence parameters and antioxidant enzymatic activities in desiccation-tolerant moss species Syntrichia ruralis (Hedw.) Web. and Mohr. Aqueous 0.001 M SA solution was sprayed on the moss cushions collected from semi-arid sandy grassland, Hungary in three seasons (spring, summer, autumn). These cushions were kept under the observation for 10 Days. Chlorophyll a fluorescence parameters, i.e., maximum photochemical quantum yield of PS II (Fv/Fm), effective photochemical quantum yield of PS II (ΦPSII), non-photochemical quenching (NPQ), and antioxidant enzymatic activities, i.e., ascorbate peroxidase (APX), catalase (CAT), guaiacol peroxidase (POD), and protein content were determined. The results showed the increase of Fv/Fm in spring and autumn season while ΦPSII was reduced significantly during spring and summer season after treatment with SA compared to control. SA-treated mosses showed higher values of non-photochemical quenching (NPQ) during the spring and autumn season than in summer. Activities of enzyme APX and CAT were found to increase in SA-treated except POD activity. In SA-treated moss cushions, lower protein content was found. It can be concluded that seasonal variation has been observed in chlorophyll fluorescence and antioxidant system after long term of desiccation in S. ruralis species that could be because of SA and might be due to fluctuations in conditions of their habitat, duration of light intensity, temperature and precipitation.

scholarly journals Pemanfaatan Pupuk Organik Kulit Pisang Dan Azolla Terhadap Pertumbuhan Kangkung Darat Dengan Sistem Akuaponik

2019 ◽  
Vol 12 (01) ◽  
pp. 1-10
Author(s):  
Maria Sastriana ◽  
Ngadiani Ngadiani
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Organic Fertilizer ◽  
Control Treatment ◽  
Banana Peel ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Azolla Pinnata ◽  
Randomized Design ◽  
Weight Test

The aim of this study was to compare the organic fertilizer of azolla pinnata and banana peel and a combination of azolla pinnata and banana peel with the same concentration of 800 ppm to the weight of harvest and the chlorophyll content of ground spinach (Ipomoea reptans Poir.). The experiment was carried out experimentally using a completely randomized design with control treatment, 800 ppm azolla pinnata, 800 ppm banana skin and a combination of azolla pinnata and banana peels 800 ppm which were repeated 6 times each. The application was carried out by pouring 800 ppm of azolla pinnata, 800 ppm of banana peel and a combination of azolla pinnata and 800 ppm banana peel on each gutter. The results showed that azolla pinnata and banana peel nutrition had a significant effect (P <0.05) on harvest weight and chlorophyll content of ground spinach plants (Ipomoea reptans Poir.). Nutrition azolla pinnata and banana peel with a concentration of 800 ppm can increase the weight of the land spinach (Ipomoea reptans Poir.) Which has the highest average. In testing the chlorophyll content, the results of chlorophyll a content, chlorophyll b content and the highest total chlorophyll content was found in azolla pinnata 800 ppm nutrition. The results of this study concluded that 800 ppm azolla pinnata, 800 ppm banana peel and 800 ppm combination of azolla pinnata and banana peel in the growth of ground spinach plants (Ipomoea reptans Poir.) Were effective to increase harvest weight and increase chlorophyll content of land spinach (Ipomoea reptans Poir.) Namely chlorophyll a, chlorophyll b and total chlorophyll of land spinach plants (Ipomoea reptans Poir.).   Keywords: ground kale, azolla pinnata, banana peel, harvest weight, test of chlorophyll content, chlorophyll a, chlorophyll b, total chlorophyll.

scholarly journals Karakterisasi Morfologi, Anatomi, dan Fisiologi Tujuh Klon Unggul Kopi Robusta

2020 ◽  
Vol 7 (2) ◽  
pp. 73
Author(s):  
Sakiroh Sakiroh ◽  
Meynarti Sari Dewi Ibrahim
Keyword(s):  
Leaf Area ◽  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Morphological Characterization ◽  
Physiological Character ◽  
Robusta Coffee ◽  
Randomized Design ◽  
Apical Leaf ◽  
Physiological Characters

<em>Morphological characterization of superior Robusta coffee plants has been widely studied, but for the anatomical and physiological character is still relatively limited. Result of this characterization is important as a basic information in order to create the new superior varieties. The purpose of this study was to investigate the morphological, anatomical, and physiological characteristics of seven clones of Robusta coffee (SA 203, BP 534, BP 42, BP 409, BP 939, BP 308 and BP 436). The experiment was conducted at Pakuwon Experimental Station and Integrated Laboratory of Indonesian Industrial and Beverage Crops Research Institute, Sukabumi, West Java, from October to November 2019.  The Completely Randomized Design with 7 Robusta coffee clones as treatments and three replications was used in this study. The variable observed were morphological (leaf area), anatomical (stomata shape and density) and physiological characters (chlorophyll a, chlorophyll b and total chlorophyll). The results showed that the leaf area of the first and third leaves of seven clones of Robusta coffee were relatively uniform. The stomata shape of Robusta coffee leaf is parasitic type (Rubiaceous) type. The SA 203 and BP 409 clones have the fewest stomata contents. The content of chlorophyll a, b, and total in the first and third leaves varies between clones. Chlorophyll content in the basal, middle, and apical leaf were relatively uniform, except clones SA 203 and BP 939 for the first leaf, and SA 203 and BP 436 for third leaf. The correlation analysis showed a positive relationship between leaf area and chlorophyll content.</em>

Chlorophyll A Fluorescence and Chlorophyll Content in Parmelia Quercina Thalli from a Polluted Region of Northern Castellon (Spain)

1996 ◽  
Vol 28 (01) ◽  
pp. 49
Author(s):  
A. Calatayud ◽  
M. J. Sanz ◽  
E. Calvo ◽  
E. Barreno ◽  
S. del Valle-Tascon
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Chlorophyll A Fluorescence

Photoprotective mechanisms during leaf ontogeny: cuticular development and anthocyanin deposition in two morphs of Agave striata that differ in leaf coloration

Botany ◽  
2009 ◽  
Vol 87 (12) ◽  
pp. 1186-1197 ◽  
Author(s):  
Nicolas Y. Fondom ◽  
Sergio Castro-Nava ◽  
Alfredo J. Huerta
Keyword(s):  
Chlorophyll A ◽  
Chlorophyll A Fluorescence ◽  
Xanthophyll Cycle ◽  
Photochemical Quenching ◽  
Delayed Response ◽  
Anthocyanin Content ◽  
Leaf Ontogeny ◽  
Leaf Coloration ◽  
Young Leaves ◽  
Non Photochemical Quenching

Our objectives were to test whether in Agave striata Zucc., a plant with crassulacean acid metabolism (CAM plant), leaf wax development is a delayed response to sunlight exposure following cutin development, and whether energy dissipation shifts from non-photochemical quenching to photochemical quenching during leaf ontogeny. Under field conditions, photosynthesis, cuticular development, and anthocyanin deposition were studied in two morphs of A. striata that differ in leaf coloration (green vs. red). We quantified leaf anthocyanin, wax, and cutin content, and also measured chlorophyll a fluorescence and leaf surface temperature. In addition, using three leaf reflectance indices, we measured relative chlorophyll and anthocyanin content, and also xanthophyll-cycle de-epoxidation state (xanthophyll conversion). Our results revealed that the main components of cuticle (wax and cutin) in leaves of A. striata are deposited during different developmental windows, which are similar to leaves of monocots such as grasses. Exposure to sunlight was found to be the most likely candidate to affect wax and anthocyanin deposition. Chlorophyll a fluorescence data revealed that the sunlight conditions experienced by both morphs predisposed the young leaves of the green morph and old leaves of both morphs to photoinhibition. Our results also revealed that old leaves of the red morph, which contain a reduced level of chlorophyll and anthocyanin, had additional photoprotection via xanthophyll conversion. The results presented here support the photoprotective function of leaf anthocyanins and wax accumulation during leaf ontogeny, indicating that their presence may compensate for the reduced dependence of non-photochemical quenching and the xanthophyll-cycle pigment conversion.

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