Diagnostics of physiological processes in living tissues by the method of optical visualization of blood pulsation

The need to ensure the possibility of widespread use of electronic and mobile health-saving technologies requires not only the formation of an appropriate information technology infrastructure and the development of effective algorithms for processing a large amount of personal information. Development of medical devices for recording physiological processes also involves the creation of innovative biologically compatible materials that allow sensors and medical sensors to work continuously in 24/7 mode. Taking into account the long-term positive experience of using large-capacity thermoplastics and elastomers in medical equipment, it seems promising to use the corresponding polymers as the main materials of wearable electronics for medical purposes. At the same time, to ensure the biological compatibility of the materials under discussion, it is necessary to minimize the possibility of the development of pathogenic microorganisms on surfaces in contact with living tissues. This type of pathogenic organisms (pathogens of a number of dangerous diseases – mycoses) includes some types of microscopic fungi - micromycetes (in particular, Aspergillus niger van Tiegem; Aspergillus terreus Thom; Penicillium cycopium Westling). The article examines the effect of surface modification by gas-phase fluorination on the nature and degree of development of a mixed colony of micromycetes on the surfaces of experimental samples made of several types of thermoplastics (polyvinyl chloride, polypropylene, low-density polyethylene, polyethylene terephthalate) and elastomers (butyl- and butadiene-nitrile rubbers, as well as ethylene, propylene and dicyclopentadiene copolymers). The nature and degree of development of colonies are quantitatively described using the original methodology developed earlier. The effect of fluorination on the nanotexture and chemical composition of the surface and near-surface layers of experimental samples was demonstrated using scanning electron microscopy (SEM) and IR Fourier spectroscopy (IRFS). The dynamics and efficiency of fluorination are described using a linearized hyperbolic model, the parameters of which are set by the least squares method.

Download Full-text

Localization of Adenosine Triphosphatase Activity in the Phleom of Nicotiana Tabacum

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100094401 ◽

1972 ◽

Vol 30 ◽

pp. 230-231

Author(s):

James Cronshaw ◽

Jamison E. Gilder

Keyword(s):

Plasma Membrane ◽

Atpase Activity ◽

Adenosine Triphosphatase ◽

Higher Plants ◽

High Surface ◽

Root Tip ◽

Animal Cells ◽

Physiological Processes ◽

Tip Cells ◽

Atpase Localization

Adenosine triphosphatase (ATPase) activity has been shown to be associated with numerous physiological processes in both plants and animal cells. Biochemical studies have shown that in higher plants ATPase activity is high in cell wall preparations and is associated with the plasma membrane, nuclei, mitochondria, chloroplasts and lysosomes. However, there have been only a few ATPase localization studies of higher plants at the electron microscope level. Poux (1967) demonstrated ATPase activity associated with most cellular organelles in the protoderm cells of Cucumis roots. Hall (1971) has demonstrated ATPase activity in root tip cells of Zea mays. There was high surface activity largely associated with the plasma membrane and plasmodesmata. ATPase activity was also demonstrated in mitochondria, dictyosomes, endoplasmic reticulum and plastids.

Download Full-text

A Scanning Electron Microscopic Study of Pro-Vascular Cellular Morphology in Chaetophora Incressata

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100119880 ◽

1985 ◽

Vol 43 ◽

pp. 638-639

Author(s):

A. E. Hotchkiss ◽

A. T. Hotchkiss ◽

R. P. Apkarian

Keyword(s):

Green Algae ◽

Vascular Plants ◽

Vascular System ◽

Higher Plants ◽

Wall Structure ◽

Electron Microscopic ◽

Physiological Processes ◽

Scanning Electron Microscopic Study ◽

Scanning Electron Microscopic ◽

Scanning Electron

Multicellular green algae may be an ancestral form of the vascular plants. These algae exhibit cell wall structure, chlorophyll pigmentation, and physiological processes similar to those of higher plants. The presence of a vascular system which provides water, minerals, and nutrients to remote tissues in higher plants was believed unnecessary for the algae. Among the green algae, the Chaetophorales are complex highly branched forms that might require some means of nutrient transport. The Chaetophorales do possess apical meristematic groups of cells that have growth orientations suggestive of stem and root positions. Branches of Chaetophora incressata were examined by the scanning electron microscope (SEM) for ultrastructural evidence of pro-vascular transport.

Download Full-text

Low-voltage field-emission SEM of polymeric membranes for glucose biosensors

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100161862 ◽

1990 ◽

Vol 48 (3) ◽

pp. 860-861

Author(s):

Lorna K. Mayo ◽

Kenneth C. Moore ◽

Mark A. Arnold

Keyword(s):

Glucose Sensor ◽

Low Voltage ◽

Glucose Sensors ◽

Polymeric Membranes ◽

Artificial Endocrine Pancreas ◽

Self Monitoring ◽

Conducting Materials ◽

Insulin Dependent ◽

Living Tissues ◽

Voltage Scanning

An implantable artificial endocrine pancreas consisting of a glucose sensor and a closed-loop insulin delivery system could potentially replace the need for glucose self-monitoring and regulation among insulin dependent diabetics. Achieving such a break through largely depends on the development of an appropriate, biocompatible membrane for the sensor. Biocompatibility is crucial since changes in the glucose sensors membrane resulting from attack by orinter action with living tissues can interfere with sensor reliability and accuracy. If such interactions can be understood, however, compensations can be made for their effects. Current polymer technology offers several possible membranes that meet the unique chemical dynamics required of a glucose sensor. Two of the most promising polymer membranes are polytetrafluoroethylene (PTFE) and silicone (Si). Low-voltage scanning electron microscopy, which is an excellent technique for characterizing a variety of polymeric and non-conducting materials, 27 was applied to the examination of experimental sensor membranes.

Download Full-text

Emotional and Physiological Processes and Positive Intervention Strategies

10.1016/s1479-3555(2003)3 ◽

2003 ◽

Keyword(s):

Intervention Strategies ◽

Physiological Processes ◽

Positive Intervention

Download Full-text

Ambulatory Assessment

European Psychologist ◽

10.1027/1016-9040.14.2.109 ◽

2009 ◽

Vol 14 (2) ◽

pp. 109-119 ◽

Cited By ~ 66

Author(s):

Ulrich W. Ebner-Priemer ◽

Timothy J. Trull

Keyword(s):

Real Time ◽

Clinical Psychology ◽

Real Life ◽

Ambulatory Assessment ◽

Computer Assisted ◽

Affective Valence ◽

Time Data ◽

Physiological Processes ◽

Context Specific ◽

Questionnaire Studies

Convergent experimental data, autobiographical studies, and investigations on daily life have all demonstrated that gathering information retrospectively is a highly dubious methodology. Retrospection is subject to multiple systematic distortions (i.e., affective valence effect, mood congruent memory effect, duration neglect; peak end rule) as it is based on (often biased) storage and recollection of memories of the original experience or the behavior that are of interest. The method of choice to circumvent these biases is the use of electronic diaries to collect self-reported symptoms, behaviors, or physiological processes in real time. Different terms have been used for this kind of methodology: ambulatory assessment, ecological momentary assessment, experience sampling method, and real-time data capture. Even though the terms differ, they have in common the use of computer-assisted methodology to assess self-reported symptoms, behaviors, or physiological processes, while the participant undergoes normal daily activities. In this review we discuss the main features and advantages of ambulatory assessment regarding clinical psychology and psychiatry: (a) the use of realtime assessment to circumvent biased recollection, (b) assessment in real life to enhance generalizability, (c) repeated assessment to investigate within person processes, (d) multimodal assessment, including psychological, physiological and behavioral data, (e) the opportunity to assess and investigate context-specific relationships, and (f) the possibility of giving feedback in real time. Using prototypic examples from the literature of clinical psychology and psychiatry, we demonstrate that ambulatory assessment can answer specific research questions better than laboratory or questionnaire studies.

Download Full-text

Monitoring of stress related physiological processes in ambulance personnel

PsycEXTRA Dataset ◽

10.1037/e516062012-047 ◽

2007 ◽

Author(s):

N. Kalezic ◽

U. Aasa ◽

M. Barnekow-Bergkvist ◽

E. Lyskov

Keyword(s):

Ambulance Personnel ◽

Physiological Processes

Download Full-text

MAGNETO-OPTICAL VISUALIZATION OF CRYSTAL TWINS IN TETRAGONAL ANTIFERROMAGNET Ca3Mn2Ge3O12

Le Journal de Physique Colloques ◽

10.1051/jphyscol:19888442 ◽

1988 ◽

Vol 49 (C8) ◽

pp. C8-967-C8-968

Author(s):

V. V. Eremenko ◽

N. F. Kharchenko ◽

S. V. Sofroneev ◽

S. L. Gnatchenko ◽

H. Le Gall ◽

...

Keyword(s):

Optical Visualization

Download Full-text

Platelet Microtubules in Clot Structure Formation and Contractile Force Generation: Investigation of a Controversy

Thrombosis and Haemostasis ◽

10.1055/s-0038-1661596 ◽

1986 ◽

Vol 56 (01) ◽

pp. 023-027 ◽

Cited By ~ 9

Author(s):

C J Jen ◽

L V McIntire

Keyword(s):

Network Formation ◽

Contractile Force ◽

Second Phase ◽

Clot Retraction ◽

Forming Process ◽

Microtubule Organization ◽

Physiological Processes ◽

Fibrin Network ◽

Two Parameters

SummaryWhether platelet microtubules are involved in clot retraction/ contraction has been controversial. To address this question we have simultaneously measured two clotting parameters, clot structural rigidity and isometric contractile force, using a rheological technique. For recalcified PRP clots these two parameters began rising together at about 15 min after CaCl2 addition. In the concentration range affecting microtubule organization in platelets, colchicine, vinca alkaloids and taxol demonstrated insignificant effects on both clotting parameters of a recalcified PRP clot. For PRP clots induced by adding small amounts of exogenous thrombin, the kinetic curves of clot rigidity were biphasic and without a lag time. The first phase corresponded to a platelet-independent network forming process, while the second phase corresponded to a platelet-dependent process. These PRP clots began generating contractile force at the onset of the second phase. For both rigidity and force parameters, only the second phase of clotting kinetics was retarded by microtubule affecting reagents. When PRP samples were clotted by adding a mixture of CaCl2 and thrombin, the second phase clotting was accelerated and became superimposed on the first phase. The inhibitory effects of micro tubule affecting reagents became less pronounced. Thrombin clotting of a two-component system (washed platelets/ purified fibrinogen) was also biphasic, with the second phase being microtubule-dependent. In conclusion, platelet microtubules are important in PRP clotted with low concentrations of thrombin, during which fibrin network formation precedes platelet-fibrin interactions. On the other hand they are unimportant if a PRP clot is induced by recalcification, during which the fibrin network is constructed in the presence of platelet-fibrin interactions. The latter is likely to be more analogous to physiological processes in vivo.

Download Full-text

COVID-19 pathophysiology: interactions of gut microbiome, melatonin, vitamin D, stress, kynurenine and the alpha 7 nicotinic receptor: Treatment implications

Melatonin Research ◽

10.32794/mr11250066 ◽

2020 ◽

Vol 3 (3) ◽

pp. 322-345 ◽

Cited By ~ 4

Author(s):

George Anderson ◽

Russel J Reiter

Keyword(s):

Vitamin D ◽

Coagulation Factors ◽

Receptor Activation ◽

Cytokine Induction ◽

Aryl Hydrocarbon ◽

Physiological Processes ◽

Pulmonary Epithelial Cells ◽

Vitamin D Levels ◽

Complex Picture ◽

Alpha 7

As data emerges on the pathophysiological underpinnings of severe acute respiratory syndrome coronavirus (SARS-CoV)-2, it is clear that there are considerable variations in its susceptibility and severity/fatality, which give indications as to its pathophysiology and treatment. SARS-CoV-2 modulatory factors include age, vitamin D levels, cigarette smoking, gender and ethnicity as well as premorbid medical conditions, including diabetes, cancer, obesity, cardiovascular disease, and immune-compromised conditions. A complex picture is emerging, with an array of systemic physiological processes interacting including circadian, immune, intestinal, CNS and coagulation factors. This article reviews data on SARS-CoV-2 pathoetiology and pathophysiology. It is proposed that a decrease in pineal and systemic melatonin is an important driver of SARS-CoV-2 susceptibility and severity, with the loss of pineal melatonin's induction of the alpha 7 nicotinic acetylcholine receptor (α7nAChR) in pulmonary epithelial cells and immune cells being a powerful regulator of susceptibility and severity, respectively. Stress, including discrimination stress, and decreased vitamin D also regulate SARS-CoV-2, including via gut dysbiosis and permeability, with a resultant decrease in the short-chain fatty acid, butyrate, and increase in circulating lipopolysaccharide. Stress and cytokine induction of the kynurenine pathways, leads to aryl hydrocarbon receptor activation, which primes platelets for heightened activity, coagulation and thrombin production, thereby driving elevations in thrombin that underpin many SARS-CoV-2 fatalities. On the basis of these pathophysiological changes, prophylactic and symptomatic treatments are proposed, including the use of melatonin and α7nAChR agonism.

Download Full-text