Exosomal proteases in colorectal cancer

The objectiveis to evaluate the level of ADAM10 and ADAM17 (a disintegrin and metalloproteinase) proteases, as well as 20S-proteasomes in blood plasma exosomes of patients with colorectal cancer.Materials and methods. The study included 60 patients with colorectal cancer (T2–4N0–2M0–1) and 10 control patients. The material for the study was EDTA blood plasma. Exosomes of blood plasma were isolated by ultrafiltration with ultracentrifugation. The level of tetraspanin-associated (ADAM10 and ADAM17) and tetraspanin-non-associated (20S-proteasome) proteases was evaluated by flow cytometry and Western blotting.Results.A twice negative subpopulation (ADAM10–/ADAM17–) predominated in blood plasma exosomes of colorectal cancer patients and control patients. The level of ADAM10+/ADAM17– exosomes was significantly higher in the exosomes of the plasma of control patients. There were no significant differences between the ADAM10/ADAM17 subpopulations and the 20S-proteasome level, depending on sex, age and tumor grade. A decrease in the ADAM10+/ADAM17– subpopulation was found in patients with metastatic colorectal cancer with hematogenous metastases compared with patients with T2–4N1–2M0 and 20S-proteasome compared to T2–4N0M0. A decrease in ADAM10–/ ADAM17+ exosomes and 20S-proteasomes level was found in exosomes of patients with colorectal cancer with a metabolic syndrome in comparison with patients without metabolic disorders.

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LRIG1 Expression and Colorectal Cancer Prognosis

10.21203/rs.3.rs-118977/v1 ◽

2020 ◽

Author(s):

Maryam Bakherad ◽

Mahdieh Salimi ◽

Seyed Abdolhamid Angaji ◽

Frouzandeh Mahjoubi ◽

Tayebeh Majidizadeh

Keyword(s):

Colorectal Cancer ◽

Prognostic Significance ◽

Prognostic Indicator ◽

Cancer Prognosis ◽

Protein Levels ◽

Tumor Tissues ◽

The Right ◽

Colorectal Cancer Patients ◽

Leucine Rich Repeats ◽

And Control

Abstract BackgroundTo make the right treatment decisions about colorectal cancer (CRC) patients reliable predictive and prognostic data are needed. However, in many cases this data is not enough. Some studies suggest that LRIG1 gene (leucine-rich repeats and immunoglobulin-like domains1) has prognostic implications in different kinds of cancers. MethodsOne hundred and two patients with colorectal cancer were retrospectively analyzed for LRIG1 expression at both mRNA and protein levels. SYBR Green Real-Time RT-PCR technique was used for mRNA expression analyses and Glyceraldehyde-3-Phosphate Dehydrogenase gene (GAPDH) was considered as a reference gene for data normalization. LRIG1 protein expression was analyzed using Immunohistochemistry (IHC). Additionally, appropriate statistic analyses were used to assess the expression of LRIG1 in test and control groups. The prognostic significance of LRIG1 expression was analyzed using the univariate and multivariate analyses.ResultsThe data revealed that the expression of LRIG1 in both mRNA and protein levels was down regulated in colorectal tumor tissues (P<0.01) but is not clinically relevant prognostic indicator in CRC. ConclusionsTherefore, it is suggested that LRIG1 expression analyses may not be considered as an important issue when making informed and individualized clinical decisions regarding the management of colorectal cancer patients.

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Expression of Migration-Related Genes in Human Colorectal Cancer and Activity of a Disintegrin and Metalloproteinase 17

BioMed Research International ◽

10.1155/2016/8208904 ◽

2016 ◽

Vol 2016 ◽

pp. 1-5 ◽

Cited By ~ 4

Author(s):

Katarzyna Walkiewicz ◽

Paweł Kozieł ◽

Martyna Bednarczyk ◽

Adam Błażelonis ◽

Urszula Mazurek ◽

...

Keyword(s):

Colorectal Cancer ◽

Prognostic Markers ◽

Microarray Data Analysis ◽

Human Colorectal Cancer ◽

Normal Tissues ◽

Significance Analysis ◽

Expression Of Genes ◽

Advanced Stages ◽

A Disintegrin And Metalloproteinase ◽

Colorectal Cancer Patients

Introduction.The ability to form metastases which depends on the mechanisms of cell migration is an important element of the progression of cancer. In the present study we analyzed the genes involved in the regulation of migration in colon cancer cells.Materials and Methods.A total of 20 pairs of surgically removed tumoral and healthy (marginal) tissues samples from colorectal cancer patients at clinical stages I-II and III-IV were analyzed. The isolation of RNA from CRC and normal tissues and its subsequent molecular analysis were performed according to manufacturer’s instructions. Microarray data analysis was performed using the GeneSpring 11.5 platform and Significance Analysis of Microarrays (SAM). In SAM analysis to identify significantly differentially expressed genes score andq-value parameters were used.Results.The largest increase in expression of genes was shown by MMP9, ADAM17, EphA2, and TIMP.Conclusions.Presented genes, especially ADAM17, MMP9, EphA2, TIMP1, ICAM 11, and CD4, may be used as prognostic markers of advanced stages of colorectal cancer, contributing to the development of new lines of therapy focused on reducing metastasis of the primary tumor.

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Calorimetry-based profiling of blood plasma from colorectal cancer patients

Biochimica et Biophysica Acta (BBA) - General Subjects ◽

10.1016/j.bbagen.2012.08.001 ◽

2012 ◽

Vol 1820 (12) ◽

pp. 1879-1885 ◽

Cited By ~ 50

Author(s):

Svetla Todinova ◽

Sashka Krumova ◽

Panayot Kurtev ◽

Valentin Dimitrov ◽

Lachezar Djongov ◽

...

Keyword(s):

Colorectal Cancer ◽

Blood Plasma ◽

Cancer Patients ◽

Colorectal Cancer Patients

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The Effect of Education Provided to Family Members Caring for Colorectal Cancer Patients on Caregiving Reactions and Healthy Lifestyle Behaviors: A Prospective Quasi-Experimental Study

10.21203/rs.3.rs-865291/v1 ◽

2021 ◽

Author(s):

nilay bektas akpınar ◽

Tulin Beduk ◽

Filiz Cay Senler

Keyword(s):

Colorectal Cancer ◽

Healthy Lifestyle ◽

Control Group ◽

Lifestyle Behaviors ◽

Healthy Lifestyle Behaviors ◽

Post Test ◽

Quasi Experimental ◽

Colorectal Cancer Patients ◽

And Control ◽

Experimental Group

Abstract Purpose: To evaluate the effects of the educational package provided to enhance family caregivers' experience of colorectal cancer patients receiving chemotherapy on healthy lifestyle and caregiving reactions.Methods: The study was conducted as a pre-test-post-test, quasi-experimental intervention with a control group. The study population consisted of 100 caregivers who provide primary care to patients with colorectal cancer. The data were collected using the "Socio-Demographic Characteristics Data Collection Form", the "Healthy Lifestyle Behaviors Scale-II", and the "Caregiver Reaction Assessment” forms. The pre-test was applied to the experimental and control groups at the first interview. After the preliminary interview, the experimental group was applied three times to face-to-face individual educational sessions through the education booklet prepared by taking the opinions of 5 academician nurses who are experts in the oncology field. The post-test then was applied to the caregivers in the experimental and control groups.Results: After the education provided to the experimental group, a positive change was observed in the mean scores of all sub-dimensions of Healthy Lifestyle Behaviors Scale-II except for the physical sub-dimension. In the control group, there was a statistically significant negative change in the post-test mean scores in all sub-dimensions of the Caregiver Reaction Assessment Scale. Lack of family support in caregivers negatively affected interpersonal relationships, nutrition, health responsibility, and spiritual growth.Conclusion: In order to reduce the problems in family members, it is important to interact with a multidisciplinary approach not only with the patient but also with the caregivers during the chemotherapy process. Clinical Trials Identifier; NCT04791982, 10.03.2021, retrospectively registered

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Abstract 2816: Discovery of a miRNA-based RT-qPCR signature able to detect early stage colorectal cancer in blood plasma

10.1158/1538-7445.am2011-2816 ◽

2011 ◽

Author(s):

Søren J. Nielsen ◽

Hanni Willenbrock ◽

Jacob U. Fog ◽

Nana Jacobsen ◽

Jan Stenvang ◽

...

Keyword(s):

Colorectal Cancer ◽

Blood Plasma ◽

Early Stage ◽

In Blood Plasma

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Prediction of overall survival through pre-operative blood plasma of colorectal cancer patients

Cytokine ◽

10.1016/j.cyto.2009.07.153 ◽

2009 ◽

Vol 48 (1-2) ◽

pp. 52-53

Author(s):

Kazuko Uno ◽

Kiyotaka Okuno ◽

Katsumi Yagi ◽

Junji Hamuro

Keyword(s):

Colorectal Cancer ◽

Overall Survival ◽

Blood Plasma ◽

Cancer Patients ◽

Colorectal Cancer Patients

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Adamalysines as Biomarkers and a Potential Target of Therapy in Colorectal Cancer Patients: Preliminary Results

Disease Markers ◽

10.1155/2019/5035234 ◽

2019 ◽

Vol 2019 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Katarzyna Walkiewicz ◽

Joanna Strzelczyk ◽

Dariusz Waniczek ◽

Krzysztof Biernacki ◽

Małgorzata Muc-Wierzgoń ◽

...

Keyword(s):

Colorectal Cancer ◽

Cancer Patients ◽

Distant Metastases ◽

Serum Levels ◽

Control Group ◽

Colorectal Cancer Patients ◽

And Control ◽

The Relationship ◽

Increased Activity

Colorectal cancer is one of the most common cancers in the world. Due to its still undetermined pathogenesis, we are searching for signaling pathways that are important in the development of colorectal cancer. In this article, we present results of study on the role of ADAM proteins in colorectal cancer. The study included 85 adult colorectal cancer patients (48 men, 37 women) and 25 patients in the control group (after diagnostic colonoscopy—without cancer). During hospitalization, a serum sample (3 cm3) was collected from the study and control group, anthropometric measurements were conducted and others clinical data were analyzed. In the serum ADAM10, 12, 17, and 28, protein concentrations were determined and, in the next step, examined the relationship between ADAMs concentrations and selected clinical parameters in both groups. The analysis showed that serum levels of ADAM10 and ADAM28 are significantly higher in patients with colorectal cancer and correlate with histopathological grading and with presence of distant metastases. Moreover, noticed the trend to correlate concentrations of adamalysines with higher BMI score. One of the functions of adamalysines is the activation of growth factors involved in cancer, including IGF and TNFα. The increased activity of adamalysines in patients may play a role in the pathogenesis of colorectal cancer. Our study highlights the prevalence of metabolic disorders in the group of patients with diagnosed CRC, and this cancer seems to be a further complication of obesity.

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