EFFECTS OF CHILLING ON THE ACCUMULATION OF ACC OXIDASE AND ACC SYNTHASE PROTEINS IN ‘GRANNY SMITH’ APPLE FRUITS

2001 ◽  
pp. 145-148
Author(s):  
I. Lara ◽  
M. Vendrell
Keyword(s):  
Acc Oxidase ◽  
Acc Synthase ◽  
Apple Fruits

scholarly journals Identifying Flavor Metabolites Under Ethylene Regulation in Apples

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 781B-781
Author(s):  
Bruno Defilippi* ◽  
Abhaya Dandekar ◽  
Adel Kader
Keyword(s):  
Headspace Analysis ◽  
Acc Oxidase ◽  
Acc Synthase ◽  
Complex Trait ◽  
Ethylene Biosynthesis ◽  
Aroma Production ◽  
Ethylene Action ◽  
Ethylene Regulation ◽  
Malic Acid Degradation ◽  
Apple Fruits

To understand the role of ethylene in overall flavor of apple fruits, ethylene production, and action were reduced using apple trees lines transformed for suppressing activity of ACC-synthase or ACC-oxidase enzymes, and 1-methylcyclopropene (1-MCP), an ethylene action inhibitor. A major reduction in ethylene biosynthesis and respiration rates was measured in fruits from these treatments. As expected, we found differential levels of dependence of flavor components on ethylene biosynthesis and action. Regarding aroma production, an ethyleneassociated event, headspace analysis showed a reduction in ester production in the ethylene-suppressed lines and in the apples treated with 1.0 μL·L-1 1-MCP for 20 hours at 20 °C. However, no major differences were observed in concentrations of alcohol and aldehyde volatiles. Other flavor metabolites that showed an ethylene-dependent pattern were organic acids and sugars. Malic acid degradation was significantly reduced under ethylene suppressed conditions, showing a recovery after exposing the fruit to ethylene. Sucrose and fructose concentrations were influenced by suppression or enhancement of ethylene. Total phenolics and individual phenolics showed an ethylene-dependent behavior only when ethylene biosynthesis was reduced, but not when ethylene action was affected. These results suggest that the regulatory mechanisms of aroma biosynthesis in apple are under partial ethylene regulation. Therefore, we are using the ethylene suppressed apple fruits study the channeling and regulation of other metabolic pathways that lead to the manifestation of a complex trait like fruit quality.

In Silico Study of Ethylene Biosynthesis: Seeking New Effectors of ACC Synthase and ACC Oxidase

2016 ◽  
Vol 11 (3) ◽  
pp. 346-356
Author(s):  
Nada Ayadi ◽  
Sarra Aloui ◽  
Rabeb Shaiek ◽  
Oussama Rokbani ◽  
Faten Raboud ◽  
...  
Keyword(s):  
In Silico ◽  
Acc Oxidase ◽  
Acc Synthase ◽  
Ethylene Biosynthesis ◽  
In Silico Study

scholarly journals Introgression of Sub1 (SUB1) QTL in mega rice cultivars increases ethylene production to the detriment of grain- filling under stagnant flooding

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sandhya Rani Kuanar ◽  
Kutubuddin Ali Molla ◽  
Krishnendu Chattopadhyay ◽  
Ramani Kumar Sarkar ◽  
Pravat Kumar Mohapatra
Keyword(s):  
Grain Yield ◽  
Ethylene Production ◽  
Stem Elongation ◽  
Starch Synthesis ◽  
Acc Oxidase ◽  
Acc Synthase ◽  
Grain Filling ◽  
Soluble Carbohydrates ◽  
Rice Cultivars ◽  
Oxidase Enzyme

AbstractIn the recent time, Submergence1 (Sub1)QTL, responsible for imparting tolerance to flash flooding, has been introduced in many rice cultivars, but resilience of the QTL to stagnant flooding (SF) is not known. The response of Sub1-introgression has been tested on physiology, molecular biology and yield of two popular rice cultivars (Swarna and Savitri) by comparison of the parental and Sub1-introgression lines (SwarnaSub1 and SavitriSub1) under SF. Compared to control condition SF reduced grain yield and tiller number and increased plant height and Sub1- introgression mostly matched these effects. SF increased ethylene production by over-expression of ACC-synthase and ACC-oxidase enzyme genes of panicle before anthesis in the parental lines. Expression of the genes changed with Sub1-introgression, where some enzyme isoform genes over-expressed after anthesis under SF. Activities of endosperm starch synthesizing enzymes SUS and AGPase declined concomitantly with rise ethylene production in the Sub1-introgressed lines resulting in low starch synthesis and accumulation of soluble carbohydrates in the developing spikelets. In conclusion, Sub1-introgression into the cultivars increased susceptibility to SF. Subjected to SF, the QTL promoted genesis of ethylene in the panicle at anthesis to the detriment of grain yield, while compromising with morphological features like tiller production and stem elongation.

scholarly journals cDNA Cloning of ACC Synthase and ACC Oxidase Genes in Cucumber Fruit and Their Differential Expression by Wounding and Auxin.

1998 ◽  
Vol 67 (5) ◽  
pp. 685-692 ◽  
Author(s):  
Shinjiro Shiomi ◽  
Mikihiro Yamamoto ◽  
Teiji Ono ◽  
Katsuya Kakiuchi ◽  
Junichi Nakamoto ◽  
...  
Keyword(s):  
Differential Expression ◽  
Cdna Cloning ◽  
Acc Oxidase ◽  
Acc Synthase ◽  
Cucumber Fruit

scholarly journals Expression of ACC Synthase and ACC Oxidase Genes in Different Tissues of Immature and Mature Cucumber Fruits.

1999 ◽  
Vol 68 (4) ◽  
pp. 830-832 ◽  
Author(s):  
Shinjiro Shiomi ◽  
Junichi Nakamoto ◽  
Mikihiro Yamamoto ◽  
Yasutaka Kubo ◽  
Reinosuke Nakamura ◽  
...  
Keyword(s):  
Acc Oxidase ◽  
Acc Synthase ◽  
Different Tissues

scholarly journals Glutathione regulates ACC synthase transcription via WRKY33 and ACC oxidase by modulating mRNA stability to induce ethylene synthesis during stress

2015 ◽  
pp. pp.01543.2015 ◽  
Author(s):  
Riddhi Datta ◽  
Deepak Kumar ◽  
Asma Sultana ◽  
Saptarshi Hazra ◽  
Dipto Bhattacharyya ◽  
...  
Keyword(s):  
Mrna Stability ◽  
Acc Oxidase ◽  
Acc Synthase ◽  
Ethylene Synthesis

ACC Oxidase and ACC Synthase Expression Profiles after Leaning of Young Radiata (P. radiata D. Don) and Maritime Pine (P. pinaster Ait.) Seedlings

2011 ◽  
Vol 31 (3) ◽  
pp. 382-391 ◽  
Author(s):  
Patricio Ramos ◽  
Claudio Valenzuela ◽  
Gregoire le Provost ◽  
Christophe Plomion ◽  
Carlos Gantz ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Acc Oxidase ◽  
Acc Synthase ◽  
Maritime Pine

scholarly journals Synergistic Effects of the Combined Application of MCP and Low O2 on Apple Fruit Ripening

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1143D-1144
Author(s):  
Mehar Asif ◽  
Prabodh Trivedi ◽  
Theophanes Solomos ◽  
Autar Mattoo
Keyword(s):  
Fruit Ripening ◽  
Similar Pattern ◽  
Synergistic Effects ◽  
Acc Oxidase ◽  
Acc Synthase ◽  
Apple Fruit ◽  
Single Application ◽  
Combined Application ◽  
Putative Receptor

We have studied the effects of MCP and low O2, applied singly and in combination, on apple fruit ripening at 1, 7, and 18 °C. The single application of 2 ppm MCP is more effective in delaying the onset of the C2H4 climacteric than is 1% O2. However, the combined application has a much larger effect than the single applications of either MCP or 1% O2. For instance, at 7 °C, the onset of the C2H4 climacteric occurs at 15, 50, and 90–95 days for the controls, 1% O2 and 2 ppm MCP, respectively, whereas the combined application of 2 ppm MCP and 1% O2 suppressed the initiation of the C2H4 climacteric for 200 days, the duration of the experiment. The retardation of the climacteric onset by the treatments is associated with the suppression of ACC-synthase (ACS1) and the putative receptor ERS1. The accumulation of their transcripts is critically dependent on the rate of C2H4 evolution. As expected, the combined application of MCP and 1% O2 completely suppressed the expression of both genes. Yet when the fruits were transferred to 18 °C in air, they ripened normally. A similar pattern of inhibition in response to the above treatments was also observed with a C2H4-dependent MAPK. The expression of ETR1, ETR2 and ACC-oxidase was not affected by the treatments. The nature of this strong effect of the combined application of MCP and low O2 is not clear. It should be pointed out that MCP does not inhibit the induction of hypoxic proteins such as ADH.

scholarly journals Physiological and Molecular Characterization of the Response to Ethylene during Senescence of Carnation Genotypic Variants

1995 ◽  
Author(s):  
William Woodson ◽  
Shimon Mayak ◽  
Haim Rabinowitch
Keyword(s):  
Ethylene Production ◽  
Dianthus Caryophyllus ◽  
Acc Oxidase ◽  
Acc Synthase ◽  
Early Response ◽  
Vase Life ◽  
Petal Senescence ◽  
Ethylene Treatment ◽  
Novel Proteins ◽  
Exogenous Ethylene

The senescence of carnation (Dianthus caryophyllus L.) flowers is associated with increased production of the phytohormone ethylene, which in turn serves to initiate and regulate the processes involved in programmed petal death. We investigated the regulation of ethylene production and petal senescence in carnation. Several carnation genotypes were identified that exhibited extended vase-life in comparison to flowers from typical commercial cultivars. The capacity of these genotypes to produce ethylene during postharvest vase-life and to respond to exogenous ethylene was investigated. Several genotypes, represented by 'Sandrosa' and 87-37G produced little ethylene durig their postharvest vase-life and as a result failed to exhibit the symptoms (in-rolling and wilting) typical of flowers producing elevated levels of ethylene. These genotypes were further separated by their capacity to respond to exogenous ethylene by both increased ethylene synthesis and premature petal senescence. In one case a genotype (799) was identified that was not capable of responding to exogenous ethylene by either increased ethylene production or premature petal senescence. The regulation of ethylene production during petal senescence was investigated both at the enzyme and gene levels. A full length cDNA was identified for the petal senescence-related ACC synthase gene. Utilizing this, and other ethylene biosynthetic pathway cDNA probes, an increase in both ACC synthase and ACC oxidase mRNAs were detected following ethylene treatment. An increase in ACC oxidase mRNA and enzyme activity was detected within 2-3 h following ethylene treatment, indicating the expression of this gene is an early response to ethylene. An investigation into the expression of novel proteins during petal senescence revealed a number of polypeptides increased in abundance and possibly play a role in the regulation or biochemical processes of senescence. One polypeptide of 70 kDa was identified as being encoded by the previously characterized gene SR12 and possibly represents a b-galactosidase involved in the remobilization of carbohydrates during senescence.

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