Roses are adapted for growth and production on acid to slightly acid soil. When grown on alkaline soil sites, without extensive soil modification and acid forming and/or iron chelate fertilization, growth is reduced and severe iron chlorosis is prevalent. This study screened 24 Rosa rootstock species and selections on one acid and two alkaline soil sites for 2 consecutive years. Plants were observed for chlorosis, chlorophyll content, fresh and dry weight production and overall quality. A final reciprocal grafting study using susceptible and tolerant selections was conducted to assure the scion could realize the adaptability of the rootstock. Overall, the following five selections consistently exhibited greater growth and decreased chlorosis on the alkaline sites: R. odorata, R. canina, R. manetii, R. sp. “Mexican”, R. fortuniana, and R. multiflora selection K-l. All other R. multiflora selections performed poorly. On the acid soil site, all rootstocks grew well. When susceptible selections were budded onto tolerant rootstocks, the scions exhibited a higher degree of tolerance. Tolerant selections budded onto susceptible rootstocks exhibited increased chlorosis and decreased growth.