COMPARATIVE ANALYSIS OF FOUR COST EFFECTIVE LABORATORY METHODS FOR QUANTIFICATION OF MICROALGAE BIOMASS IN CONTROLLED ENVIRONMENT WASTEWATER AND NUTRIENT MEDIA

Xylano-pectino-cellulolytic enzymes are valuable enzymes of the industrial sector. In our earlier study, we have reported a novel and cost effective methodology for the qualitative screening of cellulase-free xylano-pectinolytic microorganisms by replacing the commercial, highly expensive substrates with agricultural residues, but the microorganisms with xylanolytic, pectinolytic, cellulolytic, xylano-pectinolytic, xylano-cellulolytic, pectino-cellulolytic, and xylano-pectino-cellulolytic potential were obtained. The probability of getting the desired combination was low, so efforts were made to further improve this cost effective methodology for obtaining the high yield of the microbes capable of producing desired combination of enzymes. By inclusion of multiple enrichment steps in sequence, using only practically low cost substrates and without any nutrient media till primary screening stage, this improved novel protocol for screening gave only the desired microorganisms with xylano-pectino-cellulolytic activity. Using this rapid, efficient, cost effective, and improved methodology, microbes with required combination of enzymes can be obtained and the probability of getting the desired microorganisms is cent percent. This is the first report presenting the methodology for the isolation of xylano-pectino-cellulolytic positive microorganisms at low cost and consuming less time.

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Optical sensing and chemometric analysis of soil organic carbon - a cost effective alternative to conventional laboratory methods?

Soil Use and Management ◽

10.1111/j.1475-2743.2011.00337.x ◽

2011 ◽

Vol 27 (2) ◽

pp. 143-155 ◽

Cited By ~ 26

Author(s):

S. M. O’ Rourke ◽

N. M. Holden

Keyword(s):

Organic Carbon ◽

Soil Organic Carbon ◽

Optical Sensing ◽

Cost Effective ◽

Chemometric Analysis ◽

Effective Alternative ◽

Laboratory Methods ◽

Cost Effective Alternative

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Evaluation of Two Field Screening Test Kits for the Detection of PCBs in Soil by Immunoassay

Journal of AOAC International ◽

10.1093/jaoac/77.6.1664 ◽

1994 ◽

Vol 77 (6) ◽

pp. 1664-1671 ◽

Cited By ~ 4

Author(s):

Larry C Waters ◽

Rob R Smith ◽

Joe H Stewart ◽

Roger A Jenkins ◽

Richard W Counts

Keyword(s):

False Negative ◽

Cost Effective ◽

Effective Field ◽

Screening Methods ◽

Laboratory Methods ◽

Rigorous Evaluation ◽

Field Screening ◽

Hazardous Waste Sites ◽

The Cost ◽

Antigen Antibody

Abstract Effective field screening methods could minimize the time and reduce the cost of characterizing and remediating hazardous waste sites. Rigorous evaluation of novel field screening methods is required before they can be considered as replacements for, or adjuncts to, currently used laboratory methods. Alternatives to standard laboratory analytical methods should be rapid, analyte-specific, cost-effective, accurate, and sensitive in the range at which the analyte is regulated. In this study, 2 immunoassay- based field test kits for polychlorinated biphenyls (PCBs) in soil were evaluated with reference to those criteria. PCBs were analyzed in both spiked and field soil samples. Based on laboratory performance, we estimate that 20 to 40 samples can be analyzed in the field per day. Sensitivity of the assay is in the 1 ppm range. Because the assay is based on the specificity of the antigen/antibody reaction, interferences are practically negligible. The method is accurate; the false-negative and false-positive results that were observed can be explained by differences in the immunoreactivities of the Aroclors present in the test samples and the Aroclors used as standards in the assay. The savings in time and expense to analyze PCBs in soil with the immunoassay-based test kits over conventional laboratory methods should be substantial.

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Seed Treatment with Phosphonate (AG3) Suppresses Pythium Damping-off of Cucumber Seedlings

Plant Disease ◽

10.1094/pd-90-0459 ◽

2006 ◽

Vol 90 (4) ◽

pp. 459-464 ◽

Cited By ~ 22

Author(s):

P. A. Abbasi ◽

G. Lazarovits

Keyword(s):

Seed Treatment ◽

Sandy Loam ◽

Cost Effective ◽

Controlled Environment ◽

Damping Off ◽

Untreated Seed ◽

Cucumis Sativus L ◽

Muck Soil ◽

Treated Seed ◽

Loam Soil

A formulation of phosphonate (AG3) was tested as a seed treatment for the control of Pythium damping-off of cucumber (Cucumis sativus L.) plants under controlled environment and field conditions. Cucumber seed were treated by soaking for 10 min in phosphonate solution. They were then planted into peat-based mix or sandy-loam soil mixed with Pythium aphanidermatum or P. ultimum inoculum or into muck soil naturally infested with P. irregulare, P. ultimum, and other Pythium spp. Under growth-room conditions, phosphonate seed treatment provided more than 80% control of damping-off in all infested substrates tested. Effective disease control was obtained even when treated seed were stored for 5 weeks and up to 18 months prior to planting. In microplots containing naturally infested muck soil, phosphonate seed treatment decreased the percentage of diseased cucumber plants and increased total fresh weights compared with untreated seed and phosphonate post-planting drench. In field-plot tests 6 weeks after planting treated seed in Pythium-infested muck soil, cucumber stands were 63% compared with 18% in the control, which had no phosphonate exposure, and 53% in the post-planting drench. Tests for potential phytotoxicity in the greenhouse showed that radish and bok choy germination was reduced by phosphonate treatment but corn, cucumber, soybean, sugar beet, tomato, and wheat were not affected. Phosphonate seed treatment is a cost-effective way of protecting cucumber plants from Pythium damping-off.

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Comparative analysis of selected facial recognition algorithms

Nigerian Journal of Technology ◽

10.4314/njt.v39i3.31 ◽

2020 ◽

Vol 39 (3) ◽

pp. 896-904

Author(s):

J.A. Popoola ◽

C.O. Yinka-Banjo

Keyword(s):

Face Recognition ◽

Comparative Analysis ◽

Facial Recognition ◽

Research Work ◽

Cost Effective ◽

Facial Symmetry ◽

Face Matching ◽

Computer Based ◽

Haar Cascade ◽

Detection And Recognition

Systems and applications embedded with facial detection and recognition capabilities are founded on the notion that there are differences in face structures among individuals, and as such, we can perform face-matching using the facial symmetry. A widely used application of facial detection and recognition is in security. It is important that the images be processed correctly for computer-based facial recognition, hence, the usage of efficient, cost-effective algorithms and a robust database. This research work puts these measures into consideration and attempts to determine a cost-effective and reliable algorithm out of three algorithms examined. Keywords: Haar-Cascade, PCA, Eigenfaces, Fisherfaces, LBPH, Face Recognition.

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Experimental methods for studying the wetting properties of oil reservoirs: a review

Физика земли ◽

10.31857/s0002-333720193135-149 ◽

2019 ◽

pp. 135-149 ◽

Cited By ~ 1

Author(s):

A. A. Ivanova ◽

N. A. Mitiurev ◽

S. N. Shilobreeva ◽

A. N. Cheremisin

Keyword(s):

Comparative Analysis ◽

Recent Progress ◽

Experimental Methods ◽

Oil Reservoirs ◽

Practical Implementation ◽

Laboratory Methods ◽

Wetting Properties ◽

Advantages And Disadvantages ◽

Unconventional Oil ◽

Oil Formation

The theoretical principles of the laboratory methods for studying the wettability of unconventional oil formation rocks are discussed and examples of their practical implementation are presented. The comparative analysis of the advantages and disadvantages of each method is presented. It is shown that despite the recent progress in the development of methods for determining the wettability of rocks, they still need to be improved. Examples of their possible improvements are discussed.

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Comparison of Botryococcus braunii and Neochloris vigensis Biofilm Formation on Vertical Oriented Surfaces

Biointerface Research in Applied Chemistry ◽

10.33263/briac115.1284312857 ◽

2021 ◽

Vol 11 (5) ◽

pp. 12843-12857

Keyword(s):

Biofilm Formation ◽

Cell Concentration ◽

Surface Characteristics ◽

Cost Effective ◽

Botryococcus Braunii ◽

Total Proteins ◽

Chl A ◽

Algal Strain ◽

Microalgae Biomass ◽

Hydrophilic Materials

Biofilm technology is a cost-effective process for microalgae biomass production. Materials can be successfully used as microalgae biomass adhesion carriers. The productivity of two different microalgal strains, Neochloris vigensis, and Botryococcus braunii, were compared in an opened pond system on eleven different surfaces (cork, sponge towel, denim, plexiglass, stainless steel, silicone rubber, glass, geotextile, and three different patterned plexiglass). Biomass attachment on the various materials was monitored for 16 days of cultivation. Various parameters were tested during cultivation, such as pH, cell concentration, chl-a, NO3-, PO43-, lipids, total proteins, and carbohydrates. Contact angle and surface energy were used to determine the surface characteristics. Plexiglass resulted in the best performance in the case of B.braunii (28.3 g/m2), while in the case of N.vigensis, sponge towel exhibited the highest productivity (17.8 g/m2). Based on the results, the algal strain affects the attachment, and hydrophilic materials can be as efficient as hydrophobic ones.

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