Management of litchi fruit and shoot borer, Conopomorpha sinensis using organic pesticides

Litchi is an important subtropical evergreen fruit crop grown in Nepal, which have high nutritive value and refreshing taste. The Litchi growers are experiencing severe loss every year with the damage caused by several insect pests. Among them fruit and shoot borer Conopomorpha sinensis Bradley (Lepidoptera: Gracillariidae) is the one of the important insect pest. Experiments were conducted at RARS Tarahara on existing litchi orchard in RCBD design with four replication and five insecticides (Azadiractin, Chlorantraniliprole, Flubendiamide, Lambda cyhalothrin, and Dimethoate) with recommended doses in litchi orchard against C. sinensis during 2015 and 2016 AD to find out the efficient one. Among the tested insecticide chlorantraniliprole (18.5% w/w SC) and flubendiamide (39.35% m/m SC) each were found to be most efficient against C. sinensis and could be applied at the rate of 1 ml per 3 liter of water when fruit size was about pea and applied 3 times with 10 days interval. This result suggested that these two insecticides could be used for the management for C. sinensis. Farmers may reduce the losses and increase their income with the application of these insecticides

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Morphometric Analysis of the Hepatocytes from Fish Exposed to Arsenic

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100091391 ◽

1976 ◽

Vol 34 ◽

pp. 282-283

Author(s):

Elsie M. B. Sorensen

Keyword(s):

Morphometric Analysis ◽

Arsenic Concentration ◽

Ultrastructural Changes ◽

Sodium Arsenate ◽

Lepomis Cyanellus ◽

Mammalian Counterpart ◽

Intracellular Changes ◽

Green Sunfish ◽

Organic Pesticides

The detoxification capacity of the liver is well documented for a variety of substances including ethanol, organic pesticides, drugs, and metals. The piscean liver, although less enzymatically active than the mammalian counterpart (1), contains endoplasmic reticulum with an impressive repertoire of oxidizing, reducing, and conjugating abilities (2). Histopathologic changes are kncwn to occur in fish hepatocytes following in vivo exposure to arsenic (3); however, ultrastructural changes have not been reported. This study involved the morphometric analysis of intracellular changes in fish parynchymal hepatocytes and correlation with arsenic concentration in the liver.Green sunfish (Lepomis cyanellus, R.) were exposed to 0, 30, or 60 ppm arsenic (as sodium arsenate) at 20°C for 1, 2, or 3 week intervals before removal of livers for quantification of the arsenic burden (using neutron activation analysis) and morphometric analysis of ultrastructural alterations. Livers were cut into 1 mm cubes for fixation, dehydration, and embedding.

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Metabolite Differences of Polyphenols in Different Litchi Cultivars (Litchi chinensis Sonn.) Based on Extensive Targeted Metabonomics

Molecules ◽

10.3390/molecules26041181 ◽

2021 ◽

Vol 26 (4) ◽

pp. 1181

Author(s):

Nonghui Jiang ◽

Huili Zhu ◽

Wei Liu ◽

Chao Fan ◽

Feng Jin ◽

...

Keyword(s):

Main Part ◽

Ultra Performance Liquid Chromatography ◽

Total Phenol Content ◽

Phenol Content ◽

Polyphenol Content ◽

Reference Information ◽

Phenolic Components ◽

Early Maturing ◽

Litchi Fruit ◽

First Time

Litchi is an important fruit cultivated in tropical and subtropical areas with high nutritious and delicious flavor and the pulp is the main part of the fruit consumed. Previous studies found that litchi had high total phenol content and antioxidant activity, but most of them focused on the identification of single or a few phenolic components with a low throughput test, and the metabolic differences of cultivars are still unknown to a some extent. In this study we used widely targeted metabolome based on ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS/MS) to analyze the polyphenol metabolites of five different genotypes of mature litchi fruit. A total of 126 polyphenol metabolites in eight categories were identified to reveal the composition and differences of polyphenol; 15 common differential metabolites and 20 specific differential metabolites to each cultivar were found for the first time. The results infer that flavonoids, flavonols, hydroxycinnamoyls and catechins are the main polyphenol metabolites of litchi pulp. Cluster analysis showed that there were three groups of polyphenols from high to low; early maturing Feizhixiao is a kind of high polyphenol content cultivars, especially in catechins, anthocyanins, flavonols, quinic acids and hydroxycinnamoyls. The polyphenols in the flesh of mature litchi are rich, and there are significant differences among cultivars; there was a level of correlation between the contents of phenolics and the maturity of litchi cultivars; the content of phenolics in early maturing litchi cultivars appeared higher than those of mid- to late-maturing cultivars. This experiment will provide significant reference information for cultivation, breeding, processing and consumption.

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