Endometrial expression of e-cadherin in relation to estrogen-progesterone receptor characteristics of the endometrium

Aim: to evaluate the endometrial expression of e-cadherin in relation to the estrogen-progesterone-receptor status of the endometrium.Materials and methods. In total 137 women were examined including 81 patients with infertility and 40 with a history of early pregnancy loss (main group); for comparison, 16 healthy fertile women (control group) were also examined. Endometrial aspiration biopsy was performed on 6–8 days after ovulation; in parallel, a peripheral blood sample was taken to determine the levels of estradiol and progesterone. A histological and immunohistochemical study of the endometrium with determination of estrogen (ER) and progesterone (PR) receptors and e-cadherin expression was carried out.Results. All fertile women (n = 16) and only 44 % (n = 53) of patients in the main group had the normal pattern of hormonereceptor endometrial interactions, i. e., low expression of ER and PR in the endometrial glands as well as reduced expression of ER and high expression of PR in the endometrial stroma. The endometrial e-cadherin expression was assessed in the following cohorts: a) women with normal hormone-receptor interactions (16 women in the control group and 53 women in the main group); b) with abnormal variants of hormone-receptor interactions in the endometrium (68 patients in the main group). The frequency of reduced e-cadherin expression was significantly lower in the cohort of women with normal estrogenprogesterone-receptor endometrial status (a) than in the cohort of women with abnormal variants of the endometrial “response” (b): in the luminal epithelium – in 5 % (3 out of 63) (а) vs. 17 % (8 out of 47) (b) (p < 0.05); in the glands – in 13 % (9 out of 69) (a) vs. 37 % (25 out of 68) (b) (p < 0.05).Conclusion. The estrogen-progesterone-receptor status of the endometrium is significant for the e-cadherin expression in the luminal epithelium and glands of the endometrium.

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Endometrial expression of leukemia inhibitory factor in women with different estrogen/progesterone receptor status of the endometrium

Obstetrics Gynecology and Reproduction ◽

10.17749/2313-7347.2019.13.2.085-094 ◽

2019 ◽

Vol 13 (2) ◽

pp. 85-94

Author(s):

S. S. Aganezov ◽

V. N. Ellinidi ◽

A. V. Morotskaya ◽

A. S. Artemyeva ◽

A. O. Nyuganen ◽

...

Keyword(s):

Progesterone Receptor ◽

Hormone Receptor ◽

Leukemia Inhibitory Factor ◽

Pregnancy Loss ◽

Control Group ◽

Luminal Epithelium ◽

Progesterone Receptor Status ◽

Early Pregnancy Loss ◽

Receptor Interactions ◽

History Of

Aim: to analyze the endometrial expression of leukemia inhibitory factor (LIF) related to the estrogen/progesterone receptor endometrial status in women with the history of reproductive dysfunctions.Materials and methods. The main group consisted of patients with the history of infertility (n = 81) or early pregnancy loss (n = 40). The control group included 16 women with normal fertility. At days 6–8 after ovulation, endometrial biopsy was performed and peripheral blood samples were taken to assess the estradiol and progesterone levels. Histological and immunohistochemical (to quantify the estrogen (ER)/progesterone (PR) receptors and LIF expression) examinations of the endometrium biopsy materials were carried out.Results. In the middle phase of the secretion, women of the control group showed higher levels of LIF expression in the endometrial glands (94%; n = 15) and stroma (88 %; n = 14) significantly more often than patients with reproductive dysfunctions – 69 % (n = 84) and 44 % (n = 53) respectively (p < 0.05). There was no difference in the endometrial LIF expression between women with infertility and those with early pregnancy loss. In women with a sizable hormone-receptor 'response', a pronounced expression of LIF in the luminal epithelium (87 %; n = 52 out of 60) and stroma (68 %; n = 47 out of 69) was detected more often (p < 0.01) than that in patients with impaired hormone-receptor interactions (61 %, n = 27 of 44; 29 %, n = 20 of 68). High PR expression in the endometrial glands (H-score > 105) indicates the risk of impaired LIF expression in the luminal epithelium (OR =2.6) and stroma (OR = 2.5). Overexpression of ER (H-score > 155) in the endometrial stroma is associated with the risk of sub-normal LIF expression in the endometrial glands (OR = 2.5) and stroma (OR = 2.8).Conclusion. A meaningful connection has been found between the endometrial estrogen/progesterone receptor status and the expression of LIF. Women with well-pronounced endometrial hormone-receptor interactions show high levels of endometrial LIF expression more often. The stromal ER and glandular PR expression levels are considered to be prognostic factors of reduced endometrial LIF expression in the uterine body mucosa.

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Endometrial E-cadgerin Expression in Women with Reproductive Dysfunctions and Healthy Fertile Women with Ovulatory Menstrual Cycle

Translational Medicine ◽

10.18705/2311-4495-2019-6-3-44-52 ◽

2019 ◽

Vol 6 (3) ◽

pp. 44-52 ◽

Cited By ~ 1

Author(s):

S. S. Aganezov ◽

V. N. Ellinidi ◽

A. V. Morotskaya ◽

A. S. Artemyeva ◽

A. O. Nuganen ◽

...

Keyword(s):

Menstrual Cycle ◽

Endometrial Biopsy ◽

Main Group ◽

Control Group ◽

Luminal Epithelium ◽

History Of ◽

Design And Methods ◽

To Receive ◽

Fertile Women ◽

E Cadherin

Background. E-cadherin is known as one of the endometrial receptivity biomarkers.Objective: to conduct a comparative analysis of the endometrial E-cadherin expression in healthy fertile women and patients with reproductive dysfunctions with the ovulatory menstrual cycle.Design and methods. The main group consisted of patients with infertility (n = 81) and early pregnancy loss (n = 40), the control group — of 16 healthy fertile women. All subjects underwent endometrial biopsy and venipuncture to receive peripheral blood sample (to determine estradiol and progesterone levels) at 6–8 days after ovulation. Endometrial specimens were assessed by histological and immunohistochemical (evaluation of e-cadherin expression) methods.Results. In patients with reproductive failure, the frequency of apparent E-cadherin expression in the luminal (89 %, n = 84 out of 94) and glandular (74 %, n = 89 out of 121) epithelium did not differ from the corresponding indicators in fertile women (luminal epithelium — 94 %, n = 15 out of 16, gland — 88 %, n = 14 out of 16) (p > 0.05 for all indicators). The frequency of reduced E-cadherin endometrial expression was similar in patients of the main group with complete secretory transformation of the endometrium (1), incomplete secretory transformations (2) and in healthy women (3): respectively in the luminal epithelium — 7 % (n = 3 out of 43) (1), 14 % (n = 7 out of 52) (2), 6 % (n = 1 out of 16) (p > 0.05); in the glands — 20 % (n = 10 out of 51) (1), 31 % (n = 22 out of 70) (2), 13 % (n = 2 out of 16) (3) (p > 0.05).Conclusion. In patients with the history of reproductive disfunctions freaquency of lower/higher E-cadherin expression was similar to those in healthy fertile women.

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Hormone Receptor Status in Breast Cancer and its Relation to Age and Other Prognostic Factors

Breast Cancer Basic and Clinical Research ◽

10.4137/bcbcr.s7199 ◽

2011 ◽

Vol 5 ◽

pp. BCBCR.S7199 ◽

Cited By ~ 2

Author(s):

Ali Pourzand ◽

M. Bassir A. Fakhree ◽

Shahryar Hashemzadeh ◽

Monireh Halimi ◽

Amir Daryani

Keyword(s):

Breast Cancer ◽

Lymph Node ◽

Progesterone Receptor ◽

Hormone Receptor ◽

Hormone Receptor Status ◽

Progesterone Receptor Status ◽

Cross Sectional ◽

P53 Expression ◽

Receptor Status ◽

Her 2

Background Increasing evidence shows the importance of young age, estrogen receptor (ER), progesterone receptor (PR) status, and HER-2 expression in patients with breast cancers. Patients and methods We organized an analytic cross-sectional study of 105 women diagnosed with breast cancer who have been operated on between 2008 to 2010. We evaluated age, size, hormone receptor status, HER-2 and P53 expression as possible indicator of lymph node involvement. Results There is a direct correlation between positive progesterone receptor status and being younger than 40 ( P < 0.05). Also, compared with older women, young women had tumors that were more likely to be large in size and have higher stages ( P < 0.05). Furthermore patients with negative progesterone receptor status were more likely to have HER-2 overexpression ( P < 0.05). The differences in propensity to lymph node metastasis between hormone receptor statuses were not statically significant. Conclusions Although negative progesterone receptor tumors were more likely to have HER-2 overexpression, it is possible that higher stage and larger size breast cancer in younger women is related to positive progesterone receptor status.

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