The contribution of Hind III C>G PAI-1 gene polymorphism in etiology of recurrent miscarriages

Aim. Despite numerous scientific studies of possible causes of miscarriage, their etiology remains unclear in approximately 50% of cases. Investigate the prevalence of thrombophilia associated gene polymorphism FGB 455G/A, FII 20210 G/A, FV 1691G/A, ITGA2 807C/T, PAI-1 5G/4G and MTHFR 677C/T in women with sporadic and recurrent miscarriages. Methods. Group I included 35 women with sporadic miscarriage (SM), group II consisted of 57 women with recurrent miscarriage (RM) and 55 women of control group. Genetic testing was performed by PCR-RFLP. Results. In group I of women with SM the 455GA genotype of the FGB gene was more common and its presence in the genotype increases the risk of SM by 4 times and the presence of the 455A allele by 2 times. The Leiden mutation increases the risk of SM by 5 times. In II group of women with RM, the frequency of the 455 AA genotype of the FGB gene was more prevalent and the risk of RM was increased 2.5 times. It is shown that the risk of RM increases 4 times in the presence of the Leiden mutation. The 4G allele of the PAI-1 5G/4G polymorphism leads to a 2-fold increase in the risk of RM, and the presence of the 677TT genotype of the MTHFR gene increases the risk of RM by 3 times. Conclusions. Genetic factors of inherited thrombophilia alleles 455A of the FGB gene, 1691A of the FV gene, 4G of the PAI-1 gene and 677T of the MTHFR gene are alleles of significant risk of reproductive losses both sporadic and, to a greater extent, recurrent. Keywords: sporadic miscarriage, recurrent.miscarriage, inherited thrombophilia, genetic polymorphism.

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Association of a PAI-1 Gene Polymorphism and Early Life Infections with Asthma Risk, Exacerbations, and Reduced Lung Function

PLoS ONE ◽

10.1371/journal.pone.0157848 ◽

2016 ◽

Vol 11 (8) ◽

pp. e0157848 ◽

Cited By ~ 5

Author(s):

Seong H. Cho ◽

Jin-Young Min ◽

Dong Young Kim ◽

Sam S. Oh ◽

Dara R. Torgerson ◽

...

Keyword(s):

Lung Function ◽

Gene Polymorphism ◽

Early Life ◽

Asthma Risk ◽

Pai 1

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Plasma Adiponectin, T94G Gene Polymorphism and PAI-1 in Patients with and without Hypertension

Cardiology ◽

10.1159/000093610 ◽

2006 ◽

Vol 107 (1) ◽

pp. 30-37 ◽

Cited By ~ 7

Author(s):

Jing-Ren Jeng

Keyword(s):

Gene Polymorphism ◽

Plasma Adiponectin ◽

Pai 1

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Detection of plasminogen activator inhibitor-1 (-675 4G/5G) gene polymorphism in women with recurrent abortion

Hematology & Transfusion International Journal ◽

10.15406/htij.2018.07.00203 ◽

2019 ◽

Vol 7 (2) ◽

pp. 41-44

Author(s):

Iglal Youssef Shaala ◽

Akram Abdel Moneim Deghady ◽

Reham Abdel Haleem Abo Elwafa ◽

Tamer Ahmed Hosny ◽

Engy Taher Ammar

Keyword(s):

Gene Polymorphism ◽

Plasminogen Activator ◽

Pregnancy Loss ◽

Recurrent Pregnancy Loss ◽

Plasminogen Activator Inhibitor ◽

Recurrent Abortion ◽

Plasminogen Activator Inhibitor 1 ◽

Uterine Anomalies ◽

Activator Inhibitor ◽

Pai 1

Background: recurrent abortion is considered one of the most common complications that occur during pregnancy and counts for 15% of pregnancies that are recognized clinically. Many causes can be attributed to the recurrent pregnancy loss e.g. chromosomal anomalies, thrombophilic disorders, uterine anomalies, endocrine abnormalities and fetal anomalies. Thrombophilia can be either hereditary or acquired. Multiple genes had been implicated in the pathogenesis of the thrombophilia. Previous studies have indicated that genetic polymorphism of the plasminogen activator inhibitor-1 gene (PAI-1) may be associated with recurrent abortion. Aim: The aim of the present study was to investigate whether plasminogen activator inhibitor-1 (-675 4G/5G) gene polymorphism is associated with the occurrence of recurrent pregnancy loss or not. Methods: DNA samples were collected from sixty six female patients with recurrent abortion (33 primary abortion, 33 secondary abortion) and thirty four healthy controls with normal pregnancy for detection of plasminogen activator inhibitor-1 (-675 4G/5G) gene polymorphism by restriction fragment length polymorphism PCR. Results: there was a significant association between PAI-1(-675 4G/5G) polymorphism and the occurrence of recurrent pregnancy loss. Conclusion: Our results assumed that PAI-1 (-675 4G/5G) polymorphism is associated with recurrent pregnancy loss.

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Clinical impact of PAI 1 4G/5G gene polymorphism in colorectal carcinoma patients

Neoplasma ◽

10.4149/neo_2013_020 ◽

2012 ◽

Vol 60 (02) ◽

pp. 151-159 ◽

Cited By ~ 3

Author(s):

J. HALAMKOVA ◽

I. KISS ◽

Z. PAVLOVSKY ◽

J. TOMASEK ◽

J. JARKOVSKY ◽

...

Keyword(s):

Colorectal Carcinoma ◽

Gene Polymorphism ◽

Clinical Impact ◽

Pai 1

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Mcp-1, eNOS, tPA and PAI-1 Gene Polymorphism and Correlation of Genotypes and Phenotypes in Hepatopulmonary Syndrome

Digestive Diseases and Sciences ◽

10.1007/s10620-007-0002-3 ◽

2007 ◽

Vol 53 (5) ◽

pp. 1345-1351 ◽

Cited By ~ 5

Author(s):

Gokhan Tumgor ◽

Afig Berdeli ◽

Cigdem Arikan ◽

Ertürk Levent ◽

Sema Aydogdu

Keyword(s):

Gene Polymorphism ◽

Hepatopulmonary Syndrome ◽

Pai 1

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Plasminogen activator inhibitor-1 gene polymorphism as a risk factor for vascular complications in type 2 diabetes mellitus

Egyptian Journal of Medical Human Genetics ◽

10.1186/s43042-019-0018-1 ◽

2019 ◽

Vol 20 (1) ◽

Cited By ~ 1

Author(s):

Fatma A. Khalaf ◽

Hatem R. Ibrahim ◽

Hanan M. Bedair ◽

Maha M. Allam ◽

Amr A. Elshormilisy ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gene Polymorphism ◽

Vascular Complications ◽

Plasminogen Activator Inhibitor ◽

Diabetic Patients ◽

Plasminogen Activator Inhibitor 1 ◽

Increased Risk ◽

Activator Inhibitor ◽

Pai 1

Abstract Background Diabetes mellitus (DM) can lead to microvascular and macrovascular damages through hyperglycemia that is the main cause of diabetic complications. Other factors such as hypertension, obesity, and hyperlipidemia may worsen or accelerate the others. Several studies have revealed definitive genetic predispositions to the development of type 2 diabetes mellitus (T2DM) and development of vascular complications. This study aimed to address the association between plasminogen activator inhibitor-1 (PAI-1) gene polymorphism and T2DM, and if this gene polymorphism may have a possible role in the development of vascular complications in T2DM. This study is a case control; it included 200 patients with T2DM, 117 patients had no vascular complications, and 83 had previous vascular complications (VCs). One hundred eighty volunteer blood donors were selected as a healthy control group. All patients and controls were subjected to clinical examination, and laboratory investigations included lipid profile, fasting and 2 h blood glucose, complete blood cell count, d-dimer, PAI-1, thrombin activatable fibrinolysis inhibitor (TAFI), and detection of PAI-1 gene polymorphism by real-time polymerase chain reaction (PCR). Results The most prevalent genotype of PAI-1 gene polymorphism in all studied groups, including controls, was 4G/5G with the highest allele frequency as 4G. The 4G/5G and 4G/4G genotypes were associated with increased risk of DM development as compared to 5G/5G genotype. The 4G/5G and 4G/4G genotypes also had a highly significant increased risk of VCs among diabetic patients, as compared to 5G/5G. The 4G allele also was highly associated with DM with VCs. The d-dimer TAFI, PAI-1 showed the highest levels in 4G/5G genotype followed by 4G/4G genotype. The lowest level was expressed in 5G/5G genotype in diabetic patients with and without VCs. The univariable analysis showed that genotypes 4G/5G and 4G/4G were potentially risk factors for development of VCs with T2DM patients. Conclusion This study concludes that the PAI-1 4G/5G polymorphism may be associated with T2DM and may be considered as a risk factor for development of thrombotic events. It may also help in selection and dosing of patients being treated with anticoagulant and fibrinolytic agents. Further large-scale studies are recommended to assess the possible role of environmental factors and gene interactions in the development of T2DM vascular risks.

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