Histological Alterations from Condyle Repositioning with Functional Appliances in Rats

2018 ◽  
Vol 42 (5) ◽  
pp. 391-397
Author(s):  
Ji-Youn Kim ◽  
Seong-Suk Jue ◽  
Hee-Joon Bang ◽  
Hong Christine ◽  
Jong-il Moon ◽  
...  
Keyword(s):  
Morphological Analysis ◽  
Type Ii Collagen ◽  
Entire Period ◽  
Posterior Region ◽  
Anterior Region ◽  
Type Ii ◽  
Functional Appliance ◽  
Histological Changes ◽  
Histological Alterations ◽  
Functional Appliances

Objective: This study was designed to assess the morphological and histological alterations of the condyle of rats undergoing forward mandibular repositioning via functional appliance. Materials and Methods: Functional appliances were mounted onto the upper jaws of rats. Morphological analysis was conducted on micro-CT images of sacrificed animals. Histological changes in condyle were examined by immunohistochemistry using proliferating cell nuclear antigen (PCNA), matrix metalloproteases (MMPs), vascular endothelial growth factor (VEGF), tissue inhibitors of matrix metalloproteinases (TIMP-1), interleukin 1b (IL-1β), Aggrecan and Type II collagen. Osteoclast activity was identified by tartrate-resistant acid phosphatase (TRAP) staining. Results: Morphological analysis confirmed the forward positioning of the condyles of rats by the appliance, but the position gradually returned to normal on days 14 after treatment. An increase in PCNA positive cells was observed in the posterior region of the condyles on days 7, whereas PCNA positive cells decreased in the anterior region. Aggrecan and Type II collagen localization increased in the posterior region throughout the entire period, but decreased in the anterior region on days 14. In both regions, IL-1β and VEGF localization was significantly increased for 14 days while MMPs localization was evident throughout the entire period. The TRAP positive cells were significantly elevated on days 3 and 7. Conclusions: These results suggest that the functional appliance therapy induces significant morphological and histological changes in the anterior and posterior regions of the condyle and subsequently causes adaptive cellular functions such as chondrocyte differentiation and cartilage matrix formation.

Morphological revision of the Subgroup 1 Fauchald, 1970 of Marphysa de Quatrefages, 1865 (Eunicidae: Polychaeta)

Zootaxa ◽  
2018 ◽  
Vol 4480 (1) ◽  
pp. 1 ◽  
Author(s):  
ISABEL C. MOLINA-ACEVEDO
Keyword(s):  
New Species ◽  
Morphological Analysis ◽  
New Genus ◽  
Sensu Stricto ◽  
Outer Edge ◽  
Posterior Region ◽  
Anterior Region ◽  
Morphological Pattern ◽  
Size Dependent ◽  
Branchial Region

Fifteen species of Marphysa classified in the Subgroup 1 Fauchald (1970) were reviewed and evaluated in a morphological analysis of the subgroup. It was found that 13 of these have a characteristic morphological pattern distinct from that of Marphysa sensu stricto; as a consequence, a new genus is proposed, Paucibranchia n. gen. This new genus includes the species that have branchiae restricted to a few chaetigers in the anterior region, maxillae I with a rounded falcal arch and outer edge with a straight base plus a curvature in the basal inner edge, dorsal cirri longer in the branchial region and in media-posterior region as long or longer than pre-branchial chaetigers, and the postchaetal lobe in the branchial region well developed, elongated. Paucibranchia n. gen. includes six new species (P. andresi n. sp., P. carrerai n. sp., P. gathofi n. sp., P. gilberti n. sp., P. miroi n. sp. and P. patriciae n. sp.), two species not formally named, and other 13 species previously included in Marphysa (P. adenensis (Gravier, 1900) n. comb., P. bellii (Audouin & Milne-Edwards, 1833) n. comb., P. cinari (Kurt-Sahin, 2014) n. comb., P. conferta (Moore, 1911) n. comb., P. disjuncta (Hartman, 1961) n. comb., P. fallax (Marion & Bobretzky, 1875) n. comb., P. gemmata (Mohammad, 1973) n. comb., P. kinbergi (McIntosh, 1910) n. comb., P. oculata (Treadwell, 1921) n. comb., P. purcellana (Willey, 1904) n. comb., P. sinensis (Monro, 1934) n. comb., P. stragula (Grube, 1878) n. comb., P. totospinata (Lu & Fauchald, 1998) n. comb.). One species previously classified in the subgroup, Marphysa striata (Kinberg, 1865), was considered indeterminate. Finally, some statistical analyses on size dependent features and an identification key for species of the new genus were included. 

Healing of Circular Defects in the Rabbit Medial Meniscus Can Occur Spontaneously and Is not Improved by Intra-Articular Hyaluronic Acid

1996 ◽  
Vol 09 (02) ◽  
pp. 60-5 ◽  
Author(s):  
N. Hope ◽  
P. Ghosh ◽  
S. Collier
Keyword(s):  
Hyaluronic Acid ◽  
Medial Meniscus ◽  
Chondroitin Sulphate ◽  
Rabbit Model ◽  
Type Ii Collagen ◽  
Type Ii ◽  
Keratan Sulphate ◽  
Meniscal Healing ◽  
Meniscus Healing ◽  
Made In

SummaryThe aim of this study was to determine the effects of intra-articular hyaluronic acid on meniscal healing. Circular defects, 1.0 mm in diameter, were made in the anterior third of the medial meniscus in rabbits. In one joint, 0.4 ml hyaluronic acid (Healon®) was instilled, and in the contralateral (control) joint, 0.4 ml Ringer’s saline. Four rabbits were killed after four, eight and 12 weeks and the menisci examined histologically. By eight weeks most of the lesions had healed by filling with hyaline-like cartilage. Healing was not improved by hyaluronic acid treatment. The repair tissue stained strongly with alcian blue, and the presence of type II collagen, keratan sulphate, and chondroitin sulphate was demonstrated by immunohistochemical localisation. In contrast to the circular defects, longitudinal incisions made in the medial menisci of a further six rabbits did not show any healing after 12 weeks, indicating that the shape of the lesion largely determined the potential for healing.The effect of hyaluronic acid on meniscal healing was tested in a rabbit model. With one millimeter circular lesions in the medial meniscus, healing by filling with hyalinelike cartilage was not significantly affected by the application of hyaluronic acid intra-articularly at the time of surgery, compared to saline controls, as assessed histologically four, eight and 12 weeks after the operation.

PART III. AutoimmunityAn Altered Peptide Ligand of Type II Collagen Suppresses Autoimmune Arthritis

2007 ◽  
Vol 27 (4) ◽  
pp. 345-356 ◽  
Author(s):  
Linda K. Myers ◽  
Bo Tang ◽  
Edward F. Rosioniec ◽  
John M. Stuart ◽  
Andrew H. Kang
Keyword(s):  
Type Ii Collagen ◽  
Peptide Ligand ◽  
Autoimmune Arthritis ◽  
Altered Peptide Ligand ◽  
Type Ii

The Effect of Crosslinking Agents on the Properties of Type II Collagen Biomaterials

2021 ◽  
Vol 57 (4) ◽  
pp. 166-180
Author(s):  
Maria-Minodora Marin ◽  
Madalina Georgiana Albu Kaya ◽  
George Mihail Vlasceanu ◽  
Jana Ghitman ◽  
Ionut Cristian Radu ◽  
...  
Keyword(s):  
Research Work ◽  
Cartilage Tissue Engineering ◽  
Chemical Properties ◽  
Cartilage Regeneration ◽  
Type Ii Collagen ◽  
Biomechanical Properties ◽  
Cartilage Tissue ◽  
Cross Linking ◽  
Type Ii ◽  
Crosslinking Agents

Type II collagen has been perceived as the indispensable element and plays a crucial role in cartilage tissue engineering. Thus, materials based on type II collagen have drawn farther attention in both academic and research for developing new systems for the cartilage regeneration. The disadvantage of using type II collagen as a biomaterial for tissue repairing is its reduced biomechanical properties. This can be solved by physical, enzymatic or chemical cross-linking processes, which provide biomaterials with the required mechanical properties for medical applications. To enhance type II collagen properties, crosslinked collagen scaffolds with different cross-linking agents were prepared by freeze-drying technique. The present research work studied the synthesis of type II collagen biomaterials with and without crosslinking agents. Scaffolds morphology was observed by MicroCT, showing in all cases an appropriate microstructure for biological applications, and the mechanical studies were performed using compressive tests. DSC showed an increase in denaturation temperature with an increase in cross-linking agent concentration. FTIR suggested that the secondary structure of collagen is not affected after the cross-linking; supplementary, to confirm the characteristic triple-helix conformation of collagen, the CD investigation was performed. The results showed that the physical-chemical properties of type II collagen were improved by cross-linking treatments.

scholarly journals Type II Collagen-Conjugated Mesenchymal Stem Cells Micromass for Articular Tissue Targeting

Biomedicines ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 880
Author(s):  
Shamsul Bin Sulaiman ◽  
Shiplu Roy Chowdhury ◽  
Mohd Fauzi Bin Mh Busra ◽  
Rizal Bin Abdul Rani ◽  
Nor Hamdan Bin Mohamad Yahaya ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Targeted Delivery ◽  
Expression Profiles ◽  
3D Culture ◽  
Type Ii Collagen ◽  
Cartilage Defects ◽  
Target Tissue ◽  
Type Ii ◽  
Antibody Conjugation

The tissue engineering approach in osteoarthritic cell therapy often requires the delivery of a substantially high cell number due to the low engraftment efficiency as a result of low affinity binding of implanted cells to the targeted tissue. A modification towards the cell membrane that provides specific epitope for antibody binding to a target tissue may be a plausible solution to increase engraftment. In this study, we intercalated palmitated protein G (PPG) with mesenchymal stem cells (MSCs) and antibody, and evaluated their effects on the properties of MSCs either in monolayer state or in a 3D culture state (gelatin microsphere, GM). Bone marrow MSCs were intercalated with PPG (PPG-MSCs), followed by coating with type II collagen antibody (PPG-MSC-Ab). The effect of PPG and antibody conjugation on the MSC proliferation and multilineage differentiation capabilities both in monolayer and GM cultures was evaluated. PPG did not affect MSC proliferation and differentiation either in monolayer or 3D culture. The PPG-MSCs were successfully conjugated with the type II collagen antibody. Both PPG-MSCs with and without antibody conjugation did not alter MSC proliferation, stemness, and the collagen, aggrecan, and sGAG expression profiles. Assessment of the osteochondral defect explant revealed that the PPG-MSC-Ab micromass was able to attach within 48 h onto the osteochondral surface. Antibody-conjugated MSCs in GM culture is a potential method for targeted delivery of MSCs in future therapy of cartilage defects and osteoarthritis.

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