Short term human primary pulpal response after direct pulp capping with fourth-generation dentin adhesives

The purpose of this study was to evaluate the effects of the total-etch and direct pulp capping techniques on the short-term response of mechanically exposed human primary tooth pulps using three commercially available adhesive resin systems. Class V cavities were prepared on the buccal surface of intact mandibular primary molars and exposed with a carbide bur on the cavity floor. The entire cavity except the exposure site received 36% phosphoric acid gel conditioning. Exposed pulps were capped with one of the three adhesive resins, followed by restoration of the cavities with the respective restorative materials. The teeth were extracted after 60 days and prepared according to normal histological techniques. Serial sections were stained with H/B for histological evaluations. The histopathological evaluation showed that a few of the samples in the Scotchbond Multi Purpose (SMP) and Prime & Bond 2.1 (PB) groups exhibited "attempted bridge formation", while no bridge formation was evident in the other samples. Syntac Single Component (Syntac) exhibited the most severe histological response, while the mildest reactions were observed in the SMIP group. Based on the conditions of the present study, direct pulp capping with dentin bonding agents following the total-etch technique in primary teeth can not be recommended.

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Induction of reparative dentin by calcium silicate-based material as direct pulp capping agent

Bangabandhu Sheikh Mujib Medical University Journal ◽

10.3329/bsmmuj.v12i4.43857 ◽

2019 ◽

Vol 12 (4) ◽

pp. 182-186

Author(s):

Mozammal Hossain ◽

Mahmood Sajedeen ◽

Yukio Nakamura

Keyword(s):

Calcium Hydroxide ◽

Calcium Silicate ◽

Normal Function ◽

Direct Pulp Capping ◽

Pulp Tissue ◽

Pulp Capping ◽

Exposure Site ◽

Reparative Dentin ◽

Dentin Formation ◽

Reparative Dentin Formation

This study was performed to examine whether calcium silicate could induce reparative dentin formation without eliciting any adverse effect in direct pulp capping of premolar teeth. Twenty participants who need extraction of their 4 healthy permanent premolar teeth for orthodontic reasons were included in this study. Following the surgical procedure, the exposed pulp tissue was treated either with calcium silicate or covered with calcium hydroxide paste. On day 3, 7, 14 and 28, the experimental teeth was extracted and examined using light microscopy and histometric analysis to observe the inflammatory changes and the amount of reparative dentin formation. The results showed that in the calcium silicate treated teeth, substantial amounts of dentine-like tissue was formed on day 14 and mostly located on the exposure site. It was also observed in the calcium hydroxide treated teeth but dentin-like tissue located at a distance from the exposure site. The total amount of reparative dentine formed in the calcium silicate-treated teeth was significantly higher (p<0.005) than in the calcium hydroxide-treated specimens. In conclusion that the calcium silicate indices pulpal wound healing and reparative formation in the exposed teeth without affecting the normal function of the remaining pulp.

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Histomorphological response of dogs' dental pulp capped with white mineral trioxide aggregate

Brazilian Dental Journal ◽

10.1590/s0103-64402004000200004 ◽

2004 ◽

Vol 15 (2) ◽

pp. 104-108 ◽

Cited By ~ 23

Author(s):

Italo Medeiros Faraco Junior ◽

Roberto Holland

Keyword(s):

Dental Pulp ◽

Healing Process ◽

Mineral Trioxide Aggregate ◽

Morphological Aspect ◽

Bridge Formation ◽

Pulp Capping ◽

Exposure Site ◽

Pulp Inflammation ◽

White Mineral

This study was conducted to observe the response of dogs' dental pulp to white mineral trioxide aggregate (MTA) when used as pulp capping material. The pulp of 15 dogs' teeth was experimentally exposed and capped with white MTA. The animals were sacrificed two months later and the specimens were prepared for histomorphological study. The pulp capped with white MTA showed a healing process with complete dentin bridge formation in all samples. In some cases, there was not a tubular dentin shape, but only a structure with an interesting morphological aspect sealing the exposure site. Only 2 specimens exhibited pulp inflammation. In conclusion, the data obtained in this study showed that white MTA has the necessary properties of a pulp capping material.

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Histological effects of enamel matrix derivative on exposed dental pulp

Srpski arhiv za celokupno lekarstvo ◽

10.2298/sarh1508397p ◽

2015 ◽

Vol 143 (7-8) ◽

pp. 397-403 ◽

Cited By ~ 2

Author(s):

Marijana Popovic-Bajic ◽

Vesna Danilovic ◽

Branislav Prokic ◽

Bogomir Prokic ◽

Milica Manojlovic ◽

...

Keyword(s):

Healing Process ◽

Glass Ionomer Cement ◽

Enamel Matrix Derivative ◽

Tooth Pulp ◽

Control Group ◽

Direct Pulp Capping ◽

Bridge Formation ◽

Pulp Capping ◽

Tissue Reorganization ◽

And Control

Introduction. Direct pulp capping procedure is a therapeutic application of a drug on exposed tooth pulp in order to ensure the closure of the pulp chamber and to allow the healing process to take place. Objective. The aim of this study was to examine the histological effects of Emdogain? on exposed tooth pulp of a Vietnamese pig (Sus scrofa verus). Methods. The study comprised 20 teeth of a Vietnamese pig. After class V preparation on the buccal surfaces of incisors, canines and first premolars, pulp was exposed. In the experimental group, the perforations were capped with Emdogain? (Straumann, Basel, Switzerland), while in the control group pulp capping was performed with MTA? (Dentsply Tulsa Dental, Johnson City, TN, USA). All cavities were restored with glass-ionomer cement (GC Fuji VIII, GC Corporation, Tokyo, Japan). The observational period was 28 days, after which the animal was sacrificed and histological preparations were made. A light microscope was used to analyze dentin bridge formation, tissue reorganization and inflammation, and the presence of bacteria in the pulp. Results. The formation of dentin bridge was observed in the experimental and control groups. Inflammation of the pulp was mild to moderate in both groups. Angiogenesis and many odontoblast-like cells, responsible for dentin bridge formation, were observed. Necrosis was not observed in any case, nor were bacteria present in the pulp. Conclusion. Histological analysis indicated a favorable therapeutic effect of Emdogain? Gel in direct pulp capping of Vietnamese pigs. Pulp reaction was similar to that of MTA?.

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Pulpal response to different pulp capping methods after pulp exposure by air abrasion

Journal of Clinical Pediatric Dentistry ◽

10.17796/jcpd.26.3.1235wp665xmxx24u ◽

2002 ◽

Vol 26 (3) ◽

pp. 269-273 ◽

Cited By ~ 2

Author(s):

Lina Maria Cardenas-Duque ◽

Makoto Yoshida ◽

George Goto

Keyword(s):

Pediatric Dentistry ◽

Ease Of Use ◽

Control Group ◽

Capping Agent ◽

Air Abrasion ◽

Bridge Formation ◽

Pulp Capping ◽

Exposure Site ◽

Increased Risk ◽

Significant Difference

Air abrasion is regaining popularity especially in the area of pediatric dentistry due to its ease of use and its advantages. Due to the lost of tactile information, while using this technique, there is an increased risk for pulpal exposure. On the other hand, Ca(OH)2 medicament has been proven to induce dentin bridge formation, but an adequate sealing seems to be even more important that the capping material used. The purpose of this study was two fold: to assess the pulpal response after pulpal exposure by air abrasion and to evaluate the healing potential after using Ca(OH)2 medicament or Liner Bond II™ as a capping agent. Two hundred sixteen teeth from mixed-bred dogs were used in this study. The teeth were divided into three groups,A) pulpal exposure by air-abrasion followed by sealing of the cavity with Liner Bond II™, B) pulpal exposure by air-abrasion and Ca(OH)2 pulp capping and C) pulpal exposure by high-speed followed by air-abrasion and Ca(OH)2 pulp capping as a control group. The animals were sacrificed after 7, 14, 30 and 60 days and a histopathological evaluation was undertaken. After applying Analysis of Variance to compare the groups, it was observed that at earlier observation periods, the inflammatory criteria near the exposure site were different among the groups. As time elapsed, the inflammation was resolved in the pulp tissue, however, the odontoblastic layer and the dentin bridge formation had a highly statistically significant difference (p<0.001) among the various groups at all observation periods. In addition, a positive correlation was observed between the organization of the odontoblastic layer and the dentin bridge formation mainly after 30 days. It could be concluded that dentin bridge formation could be achieved with the use of Ca(OH)2 or Liner Bond II™ as capping agent with an adequate sealing. However, the formation is delayed especially when Liner Bond II™ is used as capping agent.

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Clinical, radiographic, and histologic analysis of the effects of acemannan used in direct pulp capping of human primary teeth: short-term outcomes

Odontology ◽

10.1007/s10266-015-0215-4 ◽

2015 ◽

Vol 104 (3) ◽

pp. 329-337 ◽

Cited By ~ 8

Author(s):

Siriporn Songsiripradubboon ◽

Wijit Banlunara ◽

Polkit Sangvanich ◽

Chutima Trairatvorakul ◽

Pasutha Thunyakitpisal

Keyword(s):

Primary Teeth ◽

Direct Pulp Capping ◽

Short Term ◽

Pulp Capping ◽

Histologic Analysis

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MM-MTA for Direct Pulp Capping: A Histologic Comparison with ProRoot MTA in Rat Molars

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1443 ◽

2013 ◽

Vol 14 (6) ◽

pp. 1019-1023 ◽

Cited By ~ 4

Author(s):

Issam Tanios Khalil ◽

Tony Sarkis

Keyword(s):

Light Microscopy ◽

Wistar Rats ◽

Composite Resin ◽

Direct Pulp Capping ◽

Bridge Formation ◽

Pulp Capping ◽

Significant Difference ◽

Rat Molars

ABSTRACT Aim To compare the histomorphological response of rat dental pulps capped with MM-MTA and ProRoot MTA at 1, 2 and 4 weeks postoperatively. Materials and methods Direct pulp capping with MM-MTA and ProRoot MTA, overlaid with light-cured composite resin, was performed on right and left maxillary first molars of 20 Wistar rats. Animals were killed at 1, 2 and 4 weeks postoperatively. Biopsy samples were stained and viewed by light microscopy to determine dentin bridge formation. Data were statistically evaluated with the Kruskal-Wallis test (p < 0.05). Results Absence of dentin bridge formation at 1 week, partial dentin bridge formation at 2 weeks and complete dentin bridge formation at 4 weeks are observed with MM-MTA and ProRoot MTA. The results showed no statistically significant difference between both materials at 4 weeks. Conclusion Both materials produced similar responses in the pulp when used for pulp capping in intact, caries-free molars of rats. How to cite this article Khalil IT, Sarkis T, Naaman A. MM-MTA for Direct Pulp Capping: A Histologic Comparison with ProRoot MTA in Rat Molars. J Contemp Dent Pract 2013;14(6):1019- 1023.

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Direct pulp capping using biodentine

Stomatoloski glasnik Srbije ◽

10.2298/sgs1402065p ◽

2014 ◽

Vol 61 (2) ◽

pp. 65-74

Author(s):

Marijana Popovic-Bajic ◽

Vesna Danilovic ◽

Branislav Prokic ◽

Bogomir Prokic ◽

Vukoman Jokanovic ◽

...

Keyword(s):

Healing Process ◽

Glass Ionomer Cement ◽

Control Group ◽

Therapeutic Effects ◽

Direct Pulp Capping ◽

Pulp Tissue ◽

Bridge Formation ◽

Pulp Capping ◽

Tissue Reorganization ◽

And Control

Introduction. Direct pulp capping is therapeutic method of applying medication on exposed pulp in order to allow bridge formation and healing process. The aim of this study was to investigate the effect of Biodentine on exposed dental pulp of Vietnamese pigs. Material and Methods. The study was conducted on 20 teeth of Vietnamese pigs (Sus scrofa domesticus). On buccal surfaces of incisors, canines and first premolars, class V cavities were prepared and pulp was exposed. In the experimental group (six incisors, two canines and two premolars) the perforation was covered with Biodentine? (Septodont, Saint-Maur-des-Fosses, France). In the control group, the perforation was covered with MTA? (Dentsply Tulsa Dental, Johnson City, TN, USA). All cavities were restored with glass ionomer cement (GC Fuji VIII, GC Corporation, Tokyo, Japan). Observation period was 28 days. After sacrificing the animals, histological preparations were done to analyze the presence of dentin bridge, an inflammatory reaction of the pulp, pulp tissue reorganization and the presence of bacteria. Results. Dentin bridge was observed in all teeth (experimental and control groups). Inflammation of the pulp was mild to moderate in both groups. Neoangiogenesis and many odontoblast like cells responsible for dentin bridge formation were detected. Necrosis was not observed in any case, neither the presence of Gram-positive bacteria in the pulp. Conclusion. Histological analysis indicated favorable therapeutic effects of Biodentine for direct pulp capping in teeth of Vietnamese pigs. Findings were similar with Biodentine and MTA.

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Biosealer versus Biodentine for direct pulp capping in dog’s teeth: A histopathological evaluation

10.21203/rs.2.12852/v1 ◽

2019 ◽

Author(s):

Inas M. Al-Sherbiny ◽

Mona H. Farid ◽

Ashraf M. Abu-Seida ◽

Inas Motawea ◽

Hagar A. Bastawy

Keyword(s):

Blood Vessels ◽

Tissue Response ◽

Control Group ◽

Image Analyzer ◽

Pulp Tissue ◽

Bridge Formation ◽

Pulp Capping ◽

Exposure Site ◽

Reparative Dentin ◽

Pulp Exposure

Abstract BackgroundThis study compared the effect of Biodentine (BD) and Tech Biosealer Capping (BS) on the pulpal tissue response after pulp capping in dogs, teeth.MethodsThree mongrel dogs were enrolled in this study. Class V cavities with pulp exposure were performed on the buccal surface of 30 teeth (2 experimental groups) and left without pulp exposure in 15 teeth (control group). The cavities of experimental groups were capped with either Biodentine (BD Group, N= 15 teeth) or Tech-Biosealer Capping (BS Group, N= 15 teeth). All cavities (experimental and control groups) were restored with resin-modified glass ionomer. Dentine bridge formation, architechecture of the odontoblastic layers and signs of inflammation were assessed after 1, 2 and 3 months using the computer image analyzer (Leica Qwin 500).ResultsBD group showed a thick newly formed reparative dentin bridge completely closing the exposure site with cell inclusions and mineralization, variable amount of odontoblast-like cells, preserved pulp tissue, marked numerous collagen fibers and blood vessels. While BS group showed an incomplete newly formed reparative dentin bridge with tunnel defect, vacuolated odontoblasts, complete pulp degeneration with multiple edematous spaces, hyperemic blood vessels, extravasated RBCs, multiple calcified structures scattered just beneath the dentin bridge and through the pulp tissue, and newly ill-defined odontoblasts.ConclusionFor pulp capping, Biodentine has better dentine bridge formation and pulp preservation than Tech Biosealer Capping.

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