scholarly journals Optimization of Laccase Production, and Characterization of Lignin Degradation Products by Fusarium oxysporum JUMAD-053

2021 ◽  
Vol 13 (5) ◽  
Author(s):  
Igor S. Shiraishi ◽  
Renato P. Dorte ◽  
Beatriz R Ribeiro ◽  
Gabriela C.P.S. Souza ◽  
Robert F.H. Dekker ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Lignin Degradation ◽  
Degradation Products ◽  
Laccase Production ◽  
Lignin Degradation Products

scholarly journals Characterization of Ligninolytic Bacteria and Analysis of Alkali-Lignin Biodegradation Products

2020 ◽  
Vol 69 (3) ◽  
pp. 339-347
Author(s):  
YI XIONG ◽  
YARU ZHAO ◽  
KUIKUI NI ◽  
YUE SHI ◽  
QINGFANG XU
Keyword(s):  
Metabolic Pathway ◽  
Aromatic Compounds ◽  
Lignin Degradation ◽  
Dibutyl Phthalate ◽  
Degradation Products ◽  
Lignin Biodegradation ◽  
Aromatic Esters ◽  
Degradation Ratio ◽  
Lignin Degradation Products

Ligninolytic bacteria degrading lignin were isolates and identified, and their biodegradation mechanism of alkaline-lignin was investigated. Four strains with lignin degradation capability were screened and identified from the soil, straw, and silage based on their decolorizing capacity of aniline blue and colony size on alkaline-lignin medium. The degradation ratio of Bacillus aryabhattai BY5, Acinetobacter johnsonii LN2, Acinetobacter lwoffii LN4, and Micrococcus yunnanensis CL32 have been assayed using alkaline-lignin as the unique carbon source. Further, the Lip (lignin peroxidase) and Mnp (manganese peroxidase) activities of strains were investigated. Lip activity of A. lwoffii LN4 was highest after 72 h of incubation and reached 7151.7 U · l–1. Mnp activity of M. yunnanensis CL32 was highest after 48 h and reached 12533 U · l–1. The analysis of alkaline-lignin degradation products by GC-MS revealed that the strains screened could utilize aromatic esters compounds such as dibutyl phthalate (DBP), and decomposite monocyclic aromatic compounds through the DBP aerobic metabolic pathway. The results indicate that B. aryabhattai BY5, A. johnsonii LN2, A. lwoffii LN4, and M. yunnanensis CL32 have high potential to degrade alkaline-lignin, and might utilize aromatic compounds by DBP aerobic metabolic pathway in the process of lignin degradation.

Chemical characterization and in vitro fermentation ofBrassicastraw treated with the aerobic fungus,Trametes versicolor

2011 ◽  
Vol 91 (4) ◽  
pp. 695-702 ◽  
Author(s):  
J. E. Ramirez-Bribiesca ◽  
Y. Wang ◽  
L. Jin ◽  
T. Canam ◽  
J. R. Town ◽  
...  
Keyword(s):  
Mutant Strain ◽  
Trametes Versicolor ◽  
Lignin Degradation ◽  
Volatile Fatty Acids ◽  
Degradation Products ◽  
Chemical Characterization ◽  
Gas Production ◽  
In Vitro Fermentation ◽  
Lignin Degradation Products

Ramirez-Bribiesca, J. E., Wang, Y., Jin, L., Canam, T., Town, J. R., Tsang, A., Dumonceaux, T. J. and McAllister, T. A. 2011. Chemical characterization and in vitro fermentation of Brassica straw treated with the aerobic fungus, Trametes versicolor . Can. J. Anim. Sci. 91: 695–702. Brassica napus straw (BNS) was either not treated or was treated with two strains of Trametes versicolor; 52J (wild type) or m4D (a cellobiose dehydrogenase-deficient mutant) with four treatments: (i) untreated control (C-BNS), (ii) 52J (B-52J), (iii) m4D (B-m4D) or (iv) m4D+glucose (B-m4Dg). Glucose was provided to encourage growth of the mutant strain. All treatments with T. versicolor decreased (P<0.05) neutral-detergent fibre and increased (P<0.05) protein and the concentration of lignin degradation products in straw. Ergosterol was highest (P<0.05) in straw treated with B-52J, suggesting it generated the most fungal biomass. Insoluble lignin was reduced (P<0.05) in straw treated with B-52J and B-m4D, but not with B-m4Dg. Mannose and xylose concentration were generally higher (P<0.05) in straw treated with fungi, whereas glucose and galactose were lower as compared with C-BNS. The four treatments above were subsequently assessed in rumen in vitro fermentations, along with BNS treated with 2 mL g−1of 5 N NaOH. Concentrations of total volatile fatty acids after 24 and 48h were lower (P<0.05) in incubations that contained BNS treated with T. versicolor as compared with C-BNSor NaOH-treated BNS. Compared with C-BNS, in vitrodry matter disappearance and gas production were increased (P<0.05) by NaOH, but not by treatment with either strain of T. versicolor. Although treatment with T. versicolor did release more lignin degradation products, it did not appear to provide more degradable carbohydrate to in vitro rumen microbial populations, even when a mutant strain with compromised carbohydrate metabolism was utilized. Production of secondary compounds by the aerobic fungi may inhibit rumen microbial fermentation.

Lignin degradation products as palaeoenvironmental proxies in the sediments of small lakes

2007 ◽  
Vol 38 (4) ◽  
pp. 555-567 ◽  
Author(s):  
Karol Kuliński ◽  
Joanna Święta-Musznicka ◽  
Andrzej Staniszewski ◽  
Janusz Pempkowiak ◽  
Małgorzata Latałowa
Keyword(s):  
Lignin Degradation ◽  
Degradation Products ◽  
Small Lakes ◽  
Lignin Degradation Products

Lignin and related compounds. I. A comparative study of catalysts for lignin hydrogenolysis

1969 ◽  
Vol 47 (5) ◽  
pp. 723-727 ◽  
Author(s):  
J. M. Pepper ◽  
Y. W. Lee
Keyword(s):  
Comparative Study ◽  
Raney Nickel ◽  
Lignin Degradation ◽  
Soluble Fraction ◽  
Degradation Products ◽  
Quantitative Estimation ◽  
Liquid Chromatographic Separation ◽  
Lignin Degradation Products ◽  
Liquid Chromatographic ◽  
Related Compounds

A detailed comparative study has been made of the effectiveness of various catalysts for the hydrogenolysis of spruce wood lignin. The catalysts studied were Raney nickel, 10% palladium–charcoal, 5% rhodium–charcoal, 5% rhodium–alumina, 5% ruthenium–charcoal, and 5% ruthenium–alumina. Lignin degradation products were obtained initially as a chloroform-soluble fraction which was then divided and studied as diethyl ether-soluble and -insoluble fractions. Gas–liquid chromatographic separation of the ether-soluble fraction made possible the characterization and quantitative estimation of many of the lower molecular weight lignin degradation products.The data indicate that rhodium, palladium, and a limited amount of Raney nickel produce similar results as do ruthenium and an excess of Raney nickel; however, with the latter catalysts the degradation is more severe. In particular, rhodium–charcoal and palladium–charcoal appear to offer interesting advantages as catalysts for lignin hydrogenolysis.

scholarly journals Functional acidic ionic liquids as effective solvents for the fractionation of lignocelluloses

BioResources ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. 4721-4732
Author(s):  
Shi Jia Dong ◽  
Bi Xian Zhang ◽  
Peng Zhang ◽  
Kun Yang Wu ◽  
Xin Miao He ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Lignin Degradation ◽  
Degradation Products ◽  
Reducing Sugar ◽  
Alkaline Extraction ◽  
Enzymatic Conversion ◽  
Acidic Ionic Liquids ◽  
Lignin Degradation Products ◽  
High Yields ◽  
Hydrolysis Of

There is increasing interest in the application of ionic liquids for the pretreatment and fractionation of lignocelluloses. In this study, a series of functional acidic ionic liquids (ILs) with various heterocyclic organic cations were synthesized. Corn stalks were successfully fractionated into lignin, hemicelluloses, and cellulose when ultrasonically pretreated with ILs at 70 °C for 3 h, and subsequently treated with alkaline extraction. High yields of IL-isolated lignin (18.3% to 19.6%) and (8.3% to 14.6%) were obtained using ILs in the absence and presence of water, respectively. The yield of cellulose ranged from 40.0 to 77.0% from IL treatments, whereas the yield of hemicelluloses ranged from 1.1% to 17.3%. Enzymatic hydrolysis of the isolated cellulose residual produced 89.2% to 94.9% reducing sugar with 77.8% to 86.1% glucose, which corresponded to 80.5% to 91.4% enzymatic conversion of cellulose. Syringol and vanillin were found as the main lignin degradation products.

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