scholarly journals Sensitivity of plant and bacterial test systems in detecting of mutagenic effects of oil pollution

2006 ◽  
Vol 4 (1) ◽  
pp. 22-27
Author(s):  
Petimat M Djambetova ◽  
Nina V Reutova
Keyword(s):  
Glycine Max ◽  
Ames Test ◽  
Oil Pollution ◽  
Mutagenic Effect ◽  
Test System ◽  
Test Systems ◽  
Glycine Max L ◽  
Plant Test ◽  
Mutagenic Effects ◽  
By Products

The plant test system soybean (Glycine max. (L.) Merill) line T219 turned out to be more sensitive than standart Ames test for evaluation of the mutagenic effect of soil, contaminated by products of combustion and domestic cottage processing of oil. It is preferable to use plant test systems for such investigations because they are more sensitive, simple and inexpensive in comparison with microbial ones.

scholarly journals Mutagenic effects of selected trichothecene mycotoxins and their combinations with aflatoxin B1

2013 ◽  
Vol 19 (No. 3) ◽  
pp. 90-96 ◽  
Author(s):  
P. Šmerák ◽  
I. Bárta ◽  
Z. Polívková ◽  
J. Bártová ◽  
M. Sedmíková
Keyword(s):  
Ames Test ◽  
Micronucleus Test ◽  
Mutagenic Activity ◽  
Mutagenic Effect ◽  
Low Doses ◽  
Test Systems ◽  
Strong Mutagen ◽  
The Individual ◽  
Aflatoxin B ◽  
Mutagenic Effects

The authors focused on the amplification of data on the mutagenicity of selected trichothecene mycotoxins (T-2 toxin, vomitoxin) and their combination with aflatoxin B<sub>1</sub>,which is known to be a strong mutagen. Mutagenic activity was investigated using the Ames test in a prokaryote model at low doses (close to 0.1 LD<sub>50</sub>). Whereas the individual trichothecene mycotoxins (T-2 toxin, vomitoxin) did not show any mutagenic activity in the test systems mentioned, in combination with AFB<sub>1</sub>, or as a combination of all three mycotoxins, they showed a mutagenic effect significantly greater than AFB<sub>1</sub> alone in the Ames test (in strain TA98 at all concentrations) as well as in the micronucleus test (combination of T-2 toxin with AFB<sub>1</sub>).

scholarly journals Mutagenic effects of gamma rays on soybean (Glycine max L.) germination and seedlings

2018 ◽  
Vol 102 ◽  
pp. 012059 ◽  
Author(s):  
F Kusmiyati ◽  
Sutarno ◽  
M G A Sas ◽  
B Herwibawa
Keyword(s):  
Glycine Max ◽  
Gamma Rays ◽  
Glycine Max L ◽  
Mutagenic Effects

scholarly journals GAS EMISSIONS AND MUTAGENIC EFFECTS OF DIESEL AND BIODIESEL FUELS

2018 ◽  
Vol 55 (4C) ◽  
pp. 65
Author(s):  
Phan Quang Thang
Keyword(s):  
Ames Test ◽  
Methyl Esters ◽  
Organic Pollutant ◽  
Mutagenic Effect ◽  
Waste Cooking Oil ◽  
Positive Control ◽  
Exhaust Gas ◽  
Direct Sampling ◽  
Biodiesel Fuels ◽  
Mutagenic Effects

The Ames test has been used to evaluate the mutagenic effect of exhaust gas using diesel and biodiesel fuels (BDFs) from power generator. The Salmonella typhimurium (TA98 and TA100 tester strain) were cultured because of the most sensitive for organic pollutant. The direct sampling from gas emission for Ames test was the first time proposed. Six low molecular-weight methyl esters (LMW-MEs) found in exhaust gas when using biofuels have been tested to reveal that they are not mutagenic but toxic. The quality control standards such as dimethyl sulfoxide (DMSO) and sodium azide (NaN3) were used as negative and positive control in all culture processes. DMSO is the best adsorbent to trap the pollutants in exhaust gas. The mutagenic effects of Diesel fuel (DF) with Jatropha BDF (JCO) and waste cooking oil BDF (WCO) have been evaluated. The highest mutagenicity of WCO was observed in both TA 98 and TA100 testers strain. For the same engine, the mutagenic test result is different between two kinds of BDFs. BDFs showed increased mutagenicity higher than DF with WCO>JCO>DF. The number of revertant colonies are 623>508>424 for TA100 and 66>50>41 for TA98, respectively. 

The non-mevalonate isoprenoid biosynthesis of plants as a test system for drugs against malaria and pathogenic bacteria

2000 ◽  
Vol 28 (6) ◽  
pp. 796-798 ◽  
Author(s):  
J. Zeidler ◽  
J. Schwender ◽  
C. Mueller ◽  
H. K. Lichtenthaler
Keyword(s):  
Pathogenic Bacteria ◽  
Test System ◽  
Isoprenoid Biosynthesis ◽  
Mep Pathway ◽  
Isoprenoid Pathway ◽  
Test Systems ◽  
Plant Test

Two plant test systems are presented in the search for new inhibitors of the non-mevalonate isoprenoid pathway. A derivative of clomazone appears to be an inhibitor of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate (DOXP/MEP) pathway of isoprenoid formation.

Prevalence of the pit and antipit in the genus Glycine

1987 ◽  
Vol 45 ◽  
pp. 986-987
Author(s):  
R. W. Yaklich ◽  
E. L. Vigil ◽  
W. P. Wergin
Keyword(s):  
Amino Acids ◽  
Endoplasmic Reticulum ◽  
Glycine Max ◽  
Seed Coat ◽  
Cell Types ◽  
Abaxial Surface ◽  
Legume Seed ◽  
Initial Report ◽  
Cone Cells ◽  
Glycine Max L

The legume seed coat is the site of sucrose unloading and the metabolism of imported ureides and synthesis of amino acids for the developing embryo. The cell types directly responsible for these functions in the seed coat are not known. We recently described a convex layer of tissue on the inside surface of the soybean (Glycine max L. Merr.) seed coat that was termed “antipit” because it was in direct opposition to the concave pit on the abaxial surface of the cotyledon. Cone cells of the antipit contained numerous hypertrophied Golgi apparatus and laminated rough endoplasmic reticulum common to actively secreting cells. The initial report by Dzikowski (1936) described the morphology of the pit and antipit in G. max and found these structures in only 68 of the 169 seed accessions examined.

PENGARUH PEMBERIAN PUPUK ORGANIK CAIR ASAL LIMBAH TERHADAP SERAPAN HARA SERTA PRODUKSI TANAMAN KEDELAI DI LAHAN GAMBUT

2017 ◽  
Vol 2 (02) ◽  
pp. 204-218
Author(s):  
Hendra Saputra ◽  
Intan Sari ◽  
Muhammad Arfah
Keyword(s):  
Glycine Max ◽  
Glycine Max L

Penelitian tentang pengaruh pemberian Pupuk organik cair (POC) asal limbah tumbuhan terhadap serapan hara N dan P serta produksi tanaman kedelai (Glycine max (L) Merrill) di lahan gambut telah dilaksanakan di kampus II Unisi Fakultas Pertanian Jl. Lintas Propinsi Parit 01, Desa Pulau Palas, Kecamatan Tembilahan Hulu, Kabupaten Indragiri Hilir Propinsi Riau. Dimulai dari bulan Agustus sampai bulan Oktober 2013. Tujuan dari penelitian ini untuk mendapatkan POC asal limbah tumbuhan yang terbaik untuk serapan hara N dan P serta produksi tanaman kedelai di lahan gambut. Penelitian ini menggunakan rancangan acak kelompok (RAK) faktor tunggal dengan 7 perlakuan dan 4 ulangan, 2 tanaman dijadikan sampel. Perlakuan dosis POC limbah tanaman pisang dan POC limbah sayur kol yang diberikan yaitu 0 L/Ha, 200 L/Ha, 400 L/Ha dan 600 L/Ha. Parameter pengamatan yaitu : serapan hara N dan P pada fase awal generatif, tinggi tanaman, jumlah bintil akar, polong hampa, produksi perplot, berat 100 biji dan brangkasan kering. Data pengamatan dianalisis dengan sidik ragam (ANOVA) dan dilanjutkan dengan Uji Lanjut Tukey HSD pada taraf 5%. Berdasarkan penelitian yang telah dilaksanakan dapat disimpulkan bahwa pemberian POC asal limbah tumbuhan tidak berpengaruh nyata terhadap serapan hara N dan P, tinggi tanaman, jumlah bintil akar, polong hampa, brangkasan kering tetapi berpengaruh nyata terhadap produksi perplot dan berat 100 biji.

PERTUMBUHAN DAN HASIL SEMBILAN KULTIVAR KEDELAI (Glycine max (L.) Merrrill) DI LAHAN SAWAH

2018 ◽  
Vol 1 (2) ◽  
pp. 96
Author(s):  
Siti Wahyuni ◽  
Umi Trisnaningsih ◽  
Meilina Prasetyo
Keyword(s):  
Glycine Max ◽  
Glycine Max L

Application of commercial test-systems to identify gram-negative facultatively anaerobic bacteria

2016 ◽  
Vol 3 (1) ◽  
pp. 43-48 ◽  
Author(s):  
V. Patyka ◽  
L. Butsenko ◽  
L. Pasichnyk
Keyword(s):  
Erwinia Amylovora ◽  
Anaerobic Bacteria ◽  
Biochemical Properties ◽  
Test System ◽  
Phytopathogenic Bacteria ◽  
Gram Negative ◽  
Test Systems ◽  
Facultatively Anaerobic ◽  
Microbiological Methods

Aim. To validate the suitability of commercial API 20E test-system (bioMerieux) for the identifi cation and characterization of facultative gram-negative phytopathogenic bacterial isolates. Methods. Conventional mi- crobiological methods, API 20E test-system (bioMerieux) according to the manufacturer’s instructions. Re- sults. The identifi cation results for Erwinia amylovora, Pectobacterium carotovorum and Pantoea agglome- rans isolates were derived from the conventional and API 20E test systems, which, were in line with the literature data for these species. The API 20E test-system showed high suitability for P. agglomerans isolates identifi cation. Although not all the species of facultatively anaerobic phytopathogenic bacteria may be identi- fi ed using API 20E test-system, its application will surely allow obtaining reliable data about their physiologi- cal and biochemical properties, valuable for identifi cation of bacteria, in the course of 24 h. Conclusions. The results of tests, obtained for investigated species while using API 20E test-system, and those of conventional microbiological methods coincided. The application of API 20E test-system (bioMerieux) ensures fast obtain- ing of important data, which may be used to identify phytopathogenic bacteria of Erwinia, Pectobacterium, Pantoea genera.

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