scholarly journals Application of immunoassay systems for diagnosis of dengue fever

2015 ◽  
Vol 20 (4) ◽  
pp. 8-12
Author(s):  
Yu. A Akinshina ◽  
V. F Larichev ◽  
S. G Mardanly ◽  
A. M Butenko ◽  
N. V Khutoretskaya ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Dengue Fever ◽  
Experimental Test ◽  
Igg Antibodies ◽  
West Nile Fever ◽  
West Nile ◽  
Cross Reactions ◽  
Test Systems ◽  
Tick Borne Encephalitis ◽  
Specific Igg

As a result of the application of experimental test systems "ELISA-IgM-dengue", "ELISA-IgM-WN" and "ELISA-IgM-TBE" when examining sera from patients with dengue fever (DF), West Nile fever (WNF) and tick-borne encephalitis (TBE) there was established the possibility of a clear differential diagnosis of these related flavivirus infections. Application of the ELISA-IgG test systems fail to provide such an opportunity due to cause of pronounced cross-reactions of group-IgG antibodies. In the article there are presented data on dynamics of IgM and IgG antibodies in patients with dengue fever. Detection of specific IgG in the first days of the disease may indicate to a secondary nature of infection and the possibility of the development of hemorrhagic syndrome.

scholarly journals Examination of convalescents after West Nile fever for the specific IgM, IgG and neutralizing antibodies

2016 ◽  
Vol 21 (2) ◽  
pp. 82-86
Author(s):  
Alla R. Azaryan ◽  
A. A Kozlova ◽  
A. P Grishanova ◽  
E. I Ivashchenko ◽  
G. L Shendo ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Igg Antibodies ◽  
West Nile Fever ◽  
Serum Samples ◽  
West Nile ◽  
Negative Results ◽  
Igm Elisa ◽  
Control Research ◽  
Specific Igg ◽  
Specific Igg Antibodies

The examination was executed in the Astrakhan region over 2013-2014 with participation of three laboratories. In 26 convalescents after West Nile Fever (WNF) with the serological confirmed diagnosis of WNF blood sera were examined. Neurological form of WNF was observed in 8 cases, and febrile form - in 8 convalescents. Sera have been collected in 243-358 days, on average, in 308 days after the beginning of a disease. For their examination there were used IFA-IgM ELISA (MAC-ELISA), IgG ELISA methods as well as neutralization test in Vero E6 cell culture. The results of the examination in 24 of 26 patients (92, 3%) for IgM antibodies to the virus WNF were negative. In two convalescents according to the laboratories in Astrakhan and Moscow in sera there were observed low titers of IgM (1:400) with minor indices of sera optical density (0.3 to 0.4) and negative results in the Volgograd Plague Control Research Institute. Serum samples of other two convalescents were weakly positive or questionable in testing in Volgograd, but were negative when examined in the Institute of Virology. Specific IgG antibodies were detected in 23 of 26 convalescents (88.5%), neutralizing in 22 of 24 (91.7 %). These data confirm the adequacy of the criteria and tactics for WNF serodiagnosis adopted in Russia based on the application of the MAC -ELISA (IFA - IgM).

scholarly journals IMPORTED CASES OF ARBOVIRUS INFECTIONS IN THE RUSSIAN FEDERATION

2012 ◽  
Vol 17 (1) ◽  
pp. 35-38
Author(s):  
V. F. Larichev ◽  
M. A. Saifullin ◽  
Yu. A. Akinshina ◽  
N. V. Khutoretskaya ◽  
A. M. Butenko
Keyword(s):  
Costa Rica ◽  
Dengue Fever ◽  
Unknown Origin ◽  
Immunoglobulin M ◽  
West Nile Fever ◽  
West Nile ◽  
Chikungunya Fever ◽  
Imported Cases ◽  
Antibody Capture ◽  
The Russian Federation

An immunoglobulin M (IgM) antibody capture enzyme immunoassay (MAC-ELISA) of sera from 153 patients with acute febrile diseases of unknown origin verified 60 cases of infections imported from tropical and subtropical countries to Russia, including 46 cases of dengue fever, 8 cases of Chikungunya fever, 4 cases of West Nile fever, and 2 cases of Neapolitan mosquito fever. The cases of dengue fever were associated with a visit to Indonesia (n = 22), Thailand (n = 11), Vietnam (n = 3), India (n = 3), Venezuela (n = 2), Singapore (n = 1), Sri Lanka (n = 1), Malaysia (n = 1), Costa Rica (n = 1), and Dominican Republic (n = 1). Seven dengue fever patients who had returned from Indonesia (n = 5), Thailand (n = 1), and Costa Rica (n = 1) were observed to have hemorrhagic syndrome. The patients with Chikungunya fever returned after a journey to the Maldive Islands (n = 2), Reunion Island (n = 1), India (n = 1), Indonesia (n = 2), and Singapore (n = 1). West Nile fever infection occurred in India (n = 1), Indonesia (n = 1), Costa Rica (n = 1), and Venezuela (n = 1) and mosquito fever cases were registered in the Malta and Majorca islands.

scholarly journals Scientific Conference of the Problem Commission “Arboviruses and other zoonoses viruses: Topical issues of the study of West Nile fever, dengue fever and other tropical imported arboviral infections in the Russian Federation”

2014 ◽  
Vol 19 (3) ◽  
pp. 58-61
Author(s):  
A. M Butenko
Keyword(s):  
Dengue Fever ◽  
Russian Federation ◽  
Federal State ◽  
Scientific Conference ◽  
West Nile Fever ◽  
West Nile ◽  
The Russian Federation

In the article there is presented a brief overview concerning proceedings of RAMS Problem Commission "Arboviruses and other zoonoses viruses" on the theme: "Actual issues of studying of West Nile fever, dengue fever and other tropical imported arboviral infections in the Russian Federation", which was held in Federal State Budgetary Institution "D. I. Ivanovsky Institute of Virology (Russian Federation, Moscow) on 16th of March, 2014.

scholarly journals West Nile Virus in Slovenia

Viruses ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 720
Author(s):  
Nataša Knap ◽  
Miša Korva ◽  
Vladimir Ivović ◽  
Katja Kalan ◽  
Mateja Jelovšek ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Urine Sample ◽  
Whole Genome ◽  
Igg Antibodies ◽  
West Nile Fever ◽  
West Nile ◽  
The North ◽  
Neuroinvasive Disease ◽  
First Case ◽  
Dead End

West Nile virus (WNV) is a flavivirus transmitted by mosquitoes. Birds are the reservoir for the virus; humans, horses and other mammals are dead-end hosts. Infections caused by WNV in humans can vary from asymptomatic infections to West Nile fever (WNF) or West Nile neuroinvasive disease (WNND). In 1995, a serosurvey was performed in Slovenia on forest workers, and WNV specific IgG antibodies were confirmed in 6.8% of the screened samples, indicating that WNV is circulating in Slovenia. No human disease cases were detected in Slovenia until 2013, when the first case of WNV infection was confirmed in a retrospective study in a 79-year old man with meningitis. In 2018, three patients with WNND were confirmed by laboratory tests, with detection of IgM antibodies in the cerebrospinal fluid of the patients. In one of the patients, WNV RNA was detected in the urine sample. In 2017, 2018 and 2019, a mosquito study was performed in Slovenia. Mosquitoes were sampled on 14 control locations and 35 additional locations in 2019. No WNV was detected in mosquitoes in 2017 and 2019, but we confirmed the virus in a pool of Culex sp. mosquitoes in 2018. The virus was successfully isolated, and complete genome sequence was acquired. The whole genome of the WNV was also sequenced from the patient’s urine sample. The whole genome sequences of the WNV virus detected in Slovenian patient and mosquito indicate the virus most likely spread from the north, because of the geographic proximity and because the sequences cluster with the Austrian and Hungarian sequences. A sentinel study was performed on dog sera samples, and we were able to confirm IgG antibodies in 1.8% and 4.3% of the samples in 2017 and 2018, respectively. Though Slovenia is not a highly endemic country for WNV, we have established that the virus circulates in Slovenia.

Differential diagnosis of Crimean hemorrhagic fever and West Nile fever

2021 ◽  
Vol 4_2021 ◽  
pp. 80-85
Author(s):  
Mirekina E.V. Mirekina ◽  
Galimzyanov Kh.M. Galimzyanov ◽  
Cherenova L.P. Cherenova ◽  
Sherysheva Yu.V. Sherysheva ◽  
Keyword(s):  
Differential Diagnosis ◽  
Hemorrhagic Fever ◽  
West Nile Fever ◽  
West Nile

Detection of specific IgG antibodies in blood donors and tick-borne encephalitis virus in ticks within a non-endemic area in southeast Norway

2014 ◽  
Vol 46 (3) ◽  
pp. 181-184 ◽  
Author(s):  
Astri Lervik Larsen ◽  
Anita Kanestrøm ◽  
Marthe Bjørland ◽  
Åshild Andreassen ◽  
Arnulf Soleng ◽  
...  
Keyword(s):  
Endemic Area ◽  
Blood Donors ◽  
Encephalitis Virus ◽  
Igg Antibodies ◽  
Tick Borne Encephalitis ◽  
Specific Igg ◽  
Tick Borne Encephalitis Virus ◽  
Specific Igg Antibodies

Detection of West Nile virus genome and specific antibodies in Iranian encephalitis patients

2011 ◽  
Vol 140 (8) ◽  
pp. 1525-1529 ◽  
Author(s):  
S. CHINIKAR ◽  
A. JAVADI ◽  
B. ATAEI ◽  
H. SHAKERI ◽  
M. MORADI ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Acute Flaccid Paralysis ◽  
Virus Genome ◽  
Loss Of Consciousness ◽  
Igg Antibodies ◽  
Rt Pcr ◽  
West Nile Fever ◽  
West Nile ◽  
Neuroinvasive Disease ◽  
History Of

SUMMARYWest Nile virus (WNV) is a mosquito-borne flavivirus which circulates in birds, horses and humans. An estimated 80% of WNV infections are asymptomatic. Fewer than 1% of infected persons develop neuroinvasive disease, which typically presents as encephalitis, meningitis, or acute flaccid paralysis. This study was conducted from January 2008 to June 2009 in Isfahan, Iran. Patients attending the emergency department with fever and loss of consciousness were consecutively included. Cerebrospinal fluids (CSF) were initially analysed through bacteriology and biochemistry examinations, resulting in those with evidence of meningitis being excluded. Patients' CSF and serum were diagnosed by serological and molecular assays. A total of 632 patients with fever and loss of consciousness were tested by CSF analyses. Samples of the remaining patients (39·4%) were referred for WNV investigation. Three (1·2%) of the patients were positive for both serum and CSF by RT–PCR, and six (2·4%) were positive only for IgG antibodies. History of insect bite, and blood transfusion and transplantation were risk factors for being positive by RT–PCR (P=0·048) and being IgG positive (P=0·024), respectively. The results of this study showed that the prevalence of West Nile fever is low in patients with encephalitis.

scholarly journals Arbovirus infections incidence in the south of the European part of the Russian Federation

2021 ◽  
Vol 98 (1) ◽  
pp. 84-90
Author(s):  
N. F. Vasilenko ◽  
D. A. Prislegina ◽  
O. V. Maletskaya ◽  
T. V. Taran ◽  
A. E. Platonov ◽  
...  
Keyword(s):  
Dengue Fever ◽  
Russian Federation ◽  
Viral Encephalitis ◽  
Clinical Manifestations ◽  
European Part ◽  
Epidemiological Surveillance ◽  
The South ◽  
West Nile Fever ◽  
West Nile ◽  
The Russian Federation

Purpose: analysis of the arbovirus infections incidence in the south of the European part of the Russian Federation in 2015–2019.Materials and methods. Data from statistical documentation (epidemiological examination of the infectious disease cases, reports on the arbovirus infections incidence) reported by Departments of Rospotrebnadzor to Scientific and Methodological Center for monitoring pathogens of infectious and parasitic diseases of 1–3 risk groups for subjects of the North Caucasian and Southern Federal Districts were analyzed. The obtained data were processed using Microsoft Excel 2010 program.Results. In the south of the European part of the Russian Federation Crimean-Congo hemorrhagic fever (CCHF), West Nile fever (WNF), tick-borne viral encephalitis and dengue fever cases are registered annually.An expansion of the territory with registered epidemic manifestations of CCHF and WNF was noted, reflecting an increase in the area of circulation of their pathogens. An expansion of CCHF and WNF epidemic season with the involvement into the epidemic process of people from all age groups including young children was observed. Significant increase in number of imported cases of dengue fever was documented. Markers of tick-borne viral encephalitis, Batai, Inko, Sindbis, and Tyaginya fevers were detected in residents of a number of territories. A high levels of population humoral immunity to West Nile, Batai, Inko, Sindbis Tyaginya, Ukuniemi, Bhanja, Dkhori viruses were identified in the Astrakhan region.Conclusion. The data obtained indicate the need for epidemiological surveillance both for arbovirus infections with a pronounced epidemiological and clinical manifestations, and for infections, the proportion of which in the structure of infectious pathology in southern Russia has not been sufficiently studied, but carrying a potential risk of spreading.

Specific detection and differentiation of tick‐borne encephalitis and West Nile virus induced IgG antibodies in humans and horses

2019 ◽  
Author(s):  
Alexandra Rockstroh ◽  
Beyene Moges ◽  
Beatrice S. Berneck ◽  
Tatjana Sattler ◽  
Sandra Revilla‐Fernández ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Igg Antibodies ◽  
Specific Detection ◽  
West Nile ◽  
Tick Borne Encephalitis

scholarly journals PHOSPHAN Microplate Technology-Based Microarray For Detection of IgG Antibodies against West Nile, Crimean Congo Hemorrhagic Fever and Tick-Borne Encephalitis Viruses

2009 ◽  
pp. 54-58
Author(s):  
V. G. Pomelova ◽  
T. A. Bychenkova ◽  
N. S. Ossin
Keyword(s):  
Hemorrhagic Fever ◽  
Igg Antibodies ◽  
West Nile ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Tick Borne Encephalitis ◽  
Homologous Antigen ◽  
Human Sera ◽  
Microarray Immunoassay ◽  
Tick Borne Encephalitis Virus

Demonstrated was the possibility to use PHOSPHAN microplate technology to examine human sera and detect simultaneously specific IgG antibodies to three arboviruses, including the West Nile virus (WNV), Crimean Congo Hemorrhagic Fever virus (CCHFV), and Tick-borne encephalitis virus (TBEV). Both sensitivity and specificity of microarray immunoassay approach were similar to those of ELISA tests (when serum specimens were investigated separately). Using the criterion of 2-fold or stronger reaction of the examined serum specimen with homologous antigen than with heterologous one, we succeeded in differentiation of group specific anti WNV and anti TBEV IgG antibodies in about 60% of cross reactive sera. From the economical standpoint, the PHOSPAN technology may have advantages as compared with currently used ELISAs due to miniaturization of immunoassay format and ability to multiplex.

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