A new method for isolating pure cultures

1926 ◽  
Vol 22 (5-6) ◽  
pp. 733
Author(s):  
V. Popov
Keyword(s):  
Bacterial Cell ◽  
Nutrient Medium ◽  
New Method ◽  
Experimental Animals ◽  
Liquid Nutrient Medium ◽  
Glass Slides ◽  
Pure Cultures

Peterfi and Wamoscher (Zeit. Fr Hygiene, 1926, Bd. 106, H. 1), in order to obtain pure cultures, carried out the finest manipulations with bacteria under control in Dunkelfeld: using a special chamber from glass slides and a micropipette, any bacterial cell and, transferring it to another similar chamber with a liquid nutrient medium, the process of reproduction of the microbe (Einzellkulturen) was observed under a microscope. Using this method, the authors also succeeded in infecting experimental animals with only one pneumococcal cell in a number of cases.

Meetings of medical societies; Kazan branch of the All-Russian Society of Microbiologists and Epidemiologists. Meeting 14 / XII 36

1937 ◽  
Vol 33 (7) ◽  
pp. 942-942
Author(s):  
V. Popov
Keyword(s):  
Nutrient Medium ◽  
New Method ◽  
Russian Society ◽  
Medical Societies ◽  
Pure Cultures

Dr. P.P. Horst. On a new method for isolating pure cultures of tbc bacilli.The speaker reported on the use of a nutrient medium made by the method of Assoc. Mazura for the isolation of pure cultures of TBK.

Comparative Investigation on Formation and Accumulation of Rare Phenylpropanoids in Plants and in vitro Cultures of Pimpinella major

1990 ◽  
Vol 45 (6) ◽  
pp. 602-606 ◽  
Author(s):  
B. Merkel ◽  
J. Reichling
Keyword(s):  
Callus Culture ◽  
Nutrient Medium ◽  
Callus Cultures ◽  
Comparative Investigation ◽  
In Vitro Cultures ◽  
Liquid Nutrient Medium ◽  
Whole Plants ◽  
Plant Seedlings

Abstract Unorganized callus and leaf/root-differentiating callus cultures of Pimpinella major have been established in liquid nutrient medium. Their capacity to accumulate rare phenylpropanoids such as epoxy-pseudoisoeugenol tiglate, epoxy-anol tiglate and anol tiglate was compared with that of seedlings and whole plants. The unorganized callus cultures were not able to accumulate any phenylpropanoids. In comparison, the leaf/root-differentiating callus culture promoted the accumulation of epoxy-pseudoisoeugenol tiglate (up to 90 mg/100 g fr.wt.) but not that of anol-derivatives. The accumulated amount of EPT in PMD-SH was comparable with that in plant seedlings.

scholarly journals A new method for transfer of polyethylene glycol-embedded tissue sections to silanated slides for immunocytochemistry.

1991 ◽  
Vol 39 (4) ◽  
pp. 537-540 ◽  
Author(s):  
K X Gao ◽  
J D Godkin
Keyword(s):  
Polyethylene Glycol ◽  
Colloidal Gold ◽  
Cell Structure ◽  
High Sensitivity ◽  
Antigen Detection ◽  
New Method ◽  
Retinol Binding Protein ◽  
Tissue Sections ◽  
Glass Slides ◽  
Structural Preservation

Polyethylene glycol (PEG) is an excellent embedding medium for immunohistochemical studies. It provides structural preservation superior to frozen sections and increased sensitivity of antigen detection compared with paraffin sections. One limitation of PEG embedment is that PEG sections are difficult to handle and adhere poorly to glass slides. Here we present a simple and effective method for embedding tissues in PEG and transferring the resultant sections onto silanated glass slides. In addition, a method for silver enhanced colloidal gold immunostaining was combined with common dye staining to demonstrate the excellent structure preservation and sensitive antigen detection. Bovine chorionic membrane was fixed with Bouin's fixative, embedded in polyethylene glycol (PEG) 1500, cut into 5-microns sections, flattened over agarose blocks (10 x 10 x 2 mm3), and blotted onto Digene silanated slides. Slides were then washed in PBS, which removed the PEG and agarose blocks. Tissue sections were immunocytochemically stained with dilute antiserum raised in a rabbit against purified bovine placental retinol binding protein (bpRBP). Sections were washed and incubated with 1-nm colloidal gold-labeled goat anti-rabbit IgG. The immunogold particles were enhanced by silver staining (IGSS). Specimens were observed and photographed with an Olympus epipolarization microscope. The new method offered excellent morphological preservation of cell structure and the epipolarization microscopy provided high sensitivity for detection of specific immunogold-silver particles.

scholarly journals A METHOD FOR THE PHYSIOLOGICAL STUDY OF TISSUES IN VITRO

1923 ◽  
Vol 38 (4) ◽  
pp. 407-418 ◽  
Author(s):  
Alexis Carrel
Keyword(s):  
Nutrient Medium ◽  
Bacterial Contamination ◽  
Culture Medium ◽  
Residual Activity ◽  
Physical Factors ◽  
Dynamic Changes ◽  
Fluid Medium ◽  
Continuous Growth ◽  
Pure Cultures

1. A method has been developed which allows the continuous growth of pure strains of fibroblasts, epithelium, and leucocytes in a medium which undergoes but slight spontaneous deterioration. 2. The principle of the method is to leave the tissues undisturbed while the medium is changed. This was realized by special containers allowing the change of the medium without bacterial contamination and by the simultaneous use of a solid and a fluid medium. 3. The curve of growth of pure cultures of fibroblasts and epithelial cells in a nutrient medium is a parabola; in a non-nutrient medium, it is S-shaped and expresses the residual activity of the tissues. Leucocytes invade the culture medium progressively, as do bacteria, but never aggregate in a tissue. 4. The method is used for the study of the morphological and dynamic changes occurring in tissues under the influence of chemical and physical factors.

scholarly journals Cultivation of Rhizobium leguminosarum to produce exopolysaccharide

2020 ◽  
Author(s):  
A. N. Lobanov ◽  
T. V. Polyudova
Keyword(s):  
Nutrient Medium ◽  
Rhizobium Leguminosarum ◽  
Liquid Nutrient Medium ◽  
Different Sources

While studying the bacteria Rhizobium leguminosarum from different sources, a strain was isolated. Its growth on a liquid nutrient medium is accompanied by the accumulation of a significant amount of exopolysaccharide substance.

METHODOLOGICAL APPROACHES TO THE STUDY OF FORMATION OF BIOFILMS BY CONDITIONALLY PATHOGENIC AND PATHOGENIC ENTEROSBACTERIES

2018 ◽  
Vol 1 (1) ◽  
pp. 50-58
Author(s):  
A. B. Kononenko ◽  
◽  
I. B. Pavlova ◽  
D. A. Bannikova ◽  
S. V. Britova ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Nutrient Medium ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Osmic Acid ◽  
Liquid Nutrient Medium ◽  
Petri Dishes ◽  
Peptone Broth ◽  
Scanning Electron

To study the process of biofilm formation, microorganisms were cultured in 96-well plates, on meat-peptone broth, stained with a 0,1% solution of crystalline violet for 10...15 minutes, after which the unbound dye was washed off. The quantitative accounting of the bound dye was carried out by spectrophotometry at a wavelength of 490 nm. The technique for making bacterial preparations for light and scanning electron microscopy on dodged glasses immersed in Petri dishes with a liquid nutrient medium is proposed. A suspension of bacteria at a concentration of 105 m.k/ml in a volume of 5 ml was shaken on Vortex apparatus and introduced into Petri dishes with 20 ml of meat-peptone broth. Sterile non-greased cover glasses were placed on sterile object glasses and immersed in a liquid nutrient medium in Petri dishes. The material was incubated for 18...24 hours at 37 °C. Then the cover slips were removed with tweezers and some of them were stained with 1% aqueous solution of methylene blue (for light microscopy), and some were placed in Petri dishes with bottomed filters (for electron microscopy). The latter, in order to preserve natural architectonics, were fixed in vivo by pairs of 25% glutaraldehyde for 3...5 hours. Vapors of 2...4% osmic acid solution were used for 2...3-minutes to contrast the preparations. After treatment with vapors of osmic acid, biofilms with included bacteria acquired yellowish or brown color. The obtained preparations after dehydration with propylene oxide vapors and spraying with gold ions were examined in a scanning electron microscope (SEM). The technique allows us to study the phases of development of biofilms and obtain objective data on the morphology of populations of pathogenic and conditionally pathogenic bacteria without disturbing natural architectonics. It is shown that the intensity of biofilm formation by pathogenic microorganisms, such as salmonella, Yersinia, Staphylococcus aureus was slightly higher than that of non-pathogenic: Escherichia, Proteus, Citrobacter, Enterobacter.

Stationary phase and the cell cycle of Dictyostelium discoideum in liquid nutrient medium

1976 ◽  
Vol 20 (3) ◽  
pp. 513-523 ◽  
Author(s):  
D.R. Soll ◽  
J. Yarger ◽  
M. Mirick
Keyword(s):  
Cell Cycle ◽  
Stationary Phase ◽  
Dictyostelium Discoideum ◽  
Nutrient Medium ◽  
Nuclear Dna ◽  
Cell Concentration ◽  
Phase Cell ◽  
Cell Multiplication ◽  
Mean Cell Volume ◽  
Liquid Nutrient Medium

Cells of the axenic strain of the cellular slime mould Dictyostelium discoideum, AX-3, multiply in the liquid nutrient medium HL-5 with a doubling time of 12 h. When the cell concentration reaches approximately 1 X10(7) per ml the rate of cell multiplication begins decreasing and after 20–30 h reaches zero, at a stationary phase cell concentration of 2 to 2–5 X 10(7) cells per ml. The intercept of the extrapolated log phase and stationary phase plots has arbitrarily been considered the onset of the stationary phase. We have found that after cells have been in stationary phase for 24–32 h, mean cell volume increases by 25%, average dry weight by 37%, and average protein content by 24%. These values are close to the expected values for a cell population which is blocked at a point late in the cell cycle. Stationary phase cells also contain 25% more nuclear DNA than log phase cells, indicating that the population of cells at stationary phase is blocked after the DNA replication phase. Finally, when stationary phase cells are washed free of stationary phase medium and reinoculated into fresh medium, they reinitiate cell division synchronously. In the light of the demonstrated relationship between stationary phase and the cell cycle, a possible role for the growth inhibitor produced at stationary phase is considered.

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