Embryological substantiation of the architectonics of arteriolovenular anastomoses in the human renal parenchymal bloodstream

1986 ◽  
Vol 67 (2) ◽  
pp. 138-139
Author(s):  
E. S. Valishin
Keyword(s):  
Neonatal Period ◽  
Embryonic Stage ◽  
Human Embryos ◽  
Intrauterine Development ◽  
Stage Of Development ◽  
Comprehensive Information ◽  
Human Embryos And Fetuses

The present work was undertaken to obtain comprehensive information on arteriolovenular anastomoses in the embryonic stage of development. The study was performed on 240 cadavers of human embryos and fetuses from 6.5 weeks of intrauterine development to the neonatal period using conventional injectable and noninjectable angiological and histological methods.

scholarly journals Development of sacral vertebrae in human embryos and fetuses

2020 ◽  
Vol 9 (3) ◽  
pp. 22-27
Author(s):  
S. L. Kabak ◽  
V. V. Zatochnaya
Keyword(s):  
Spinal Column ◽  
Human Embryos ◽  
Serial Sections ◽  
Lumbosacral Region ◽  
Intrauterine Development ◽  
Alizarin Red ◽  
Fetal Spine ◽  
Human Embryos And Fetuses ◽  
Hematoxylin And Eosin ◽  
Medical University

The aim of the study is to define the features of organogenesis and histogenesis of sacral vertebrae in human embryos, which predetermine the anatomical structure of the sacrum in postnatal ontogenesis. Material and methods. The authors studied human embryo serial sections at 17–23 stages from the Carnegie virtual embryological collection, total preparations and serial sections of the lumbosacral region of the fetal spine at 8–20 weeks of gestation without visible developmental anomalies. Histological sections were stained with hematoxylin and eosin; total preparations were stained with alizarin red and alcian blue. In addition, the study included 19 adult sacra from the anatomical collection of the Belarusian State Medical University. Results. It was found that consolidation of the anlages of the upper sacral vertebrae starts at the early stages of embryogenesis by the fusion of their costal-transverse processes. During the first half of intrauterine development, the arch and articular processes of the adjacent vertebrae remain separate from each other. In contrast to other segments of the spinal column, five primary centers of ossification are formed in the anlages of the upper sacral vertebrae

scholarly journals Early life stress during the neonatal period alters social play and Line1 during the juvenile stage of development

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Amelia Cuarenta ◽  
Stacey L. Kigar ◽  
Ian C. Henion ◽  
Liza Chang ◽  
Vaishali P. Bakshi ◽  
...  
Keyword(s):  
Life Stress ◽  
Early Life ◽  
Copy Number ◽  
Neonatal Period ◽  
Early Life Stress ◽  
Social Play ◽  
Psychological Disorders ◽  
Dna Copy Number ◽  
Juvenile Period ◽  
Stage Of Development

AbstractEarly life stress (ELS) has been shown to have a significant impact on typical brain development and the manifestation of psychological disorders through epigenetic modifications that alter gene expression. Line1, a retrotransposon associated with genetic diversity, has been linked with various psychological disorders that are associated with ELS. Our previous work demonstrated altered Line1 DNA copy number in the neonatal period following stressful experiences; we therefore chose to investigate whether early life stress altered Line1 retrotransposition persists into the juvenile period of development. Our study uses a neonatal predator odor exposure (POE) paradigm to model ELS in rats. We examined Line1 using qPCR to assess Line1 expression levels and DNA copy number in the male and female juvenile amygdala, hippocampus and prefrontal cortex—areas chosen for their association with affective disorders and stress. We report a sex difference in Line1 levels within the juvenile amygdala. We also find that ELS significantly increases Line1 DNA copy number within the juvenile amygdala which correlates with reduced juvenile social play levels, suggesting the possibility that Line1 may influence juvenile social development.

The development of the dorsal mesentery in human embryos and fetuses

2019 ◽  
Vol 92 ◽  
pp. 18-26 ◽  
Author(s):  
Jill P.J.M. Hikspoors ◽  
Nutmethee Kruepunga ◽  
Greet M.C. Mommen ◽  
Jean-Marie P.W.U. Peeters ◽  
Cindy J.M. Hülsman ◽  
...  
Keyword(s):  
Human Embryos ◽  
Human Embryos And Fetuses ◽  
Dorsal Mesentery

scholarly journals LECTIN-HISTOCHEMICAL REGULARITIES OF DIFFERENTIATION OF THE HUMAN PAROTID GLAND EPITHELIAL GERMS

2018 ◽  
Vol 17 (3) ◽  
pp. 44-50
Author(s):  
О. М. Slobodian ◽  
L. P. Lavriv ◽  
D. B. Stoliar ◽  
I. S. Kashperuk-Karpiuk ◽  
L. M. Rak
Keyword(s):  
Parotid Gland ◽  
Helix Pomatia ◽  
Living Organism ◽  
Basal Membrane ◽  
Human Embryos ◽  
Intrauterine Development ◽  
Sambucus Nigra Agglutinin ◽  
Laburnum Anagyroides ◽  
Prenatal Ontogenesis ◽  
Histochemical Examination

Glycopolymer compounds make up structural and functional basis of cells and tissues of a living organism. The necessity of anatomical-lectinohistochemical examination of the parotid gland in early prenatal period of ontogenesis is substantiated, as the evidences concerning its topography are fragmentary and not systematized, and certain aspects of its ontogenesis are disputable. 35 human embryos and pre-fetuses aged from 21 days to 12 weeks of intrauterine development were examined. Glycopolymers were found by means of treatment of serial sections with lectins conjugated with horseradish peroxidase. The dynamics of prenatal morphogenesis of the embryos and pre-fetuses of the 4th-12th week of embryogenesis expression of glycopolymers – lectinreceptors on the surface of cells, in the cytoplasm and on the basal membrane of the parotid gland epithelial germs and the human oral cavity with its derivatives by re-distribution of glycopolymers are similar, which can be the evidence of ectodermal source of the parotid gland epithelial germ. Immersion of the epithelial cells of the buccal-alveolar pockets into the lower adjacent mesenchyme with the formation of primary parotid gland germs in embryos with 11,0-12,5 mm of PCL and their transformation into the epithelial taeniae is associated with accumulation of sialic glycopolymers (N-acetyl neuraminic acid), N-acetyl-D-glucosamine – specific to Wheat germ agglutinin (WGA) and Sambucus nigra agglutinin (SNA); N-acetyl-2-desoxy-2-amino-D-glucopyranose, screened by sialic acid of ß-D-galactose and α-L-fucose – specific to Helix pomatia (edible snail) agglutinin (HPA),  Rhizoctonia solani agglutinin (RCA) and Laburnum anagyroides (Golden Rain shrub) agglutinin (LABA) respectively. These glycopolymers are present during the first 12 weeks both on the cytolemma of the cells of the parotid gland epithelial germ and in their cytoplasm.   The results of lectin-histochemical examination of the early prenatal ontogenesis of the parotid gland can form the basis for the work of laboratories dealing with screening of morphological material in order to assess the degree of maturation and prognosis of fetus viability and diagnostics of deviations from normal development.

Adaptive capabilities of newborns depending on the conditions of intrauterine development

1982 ◽  
Vol 63 (4) ◽  
pp. 5-7
Author(s):  
E. A. Efimova ◽  
S. L. Nesterov ◽  
N. L. Yashina ◽  
T. K. Shakurova ◽  
V. A. Novikova ◽  
...  
Keyword(s):  
Blood Cells ◽  
Neonatal Period ◽  
Preventive Therapy ◽  
First Year ◽  
Children At Risk ◽  
Intrauterine Development ◽  
Adaptive Capabilities ◽  
First Year Of Life ◽  
Adaptive Reactions

Clinical, cytochemical, and rheological features of the neonatal period were studied in 460 children from mothers suffering from rheumatism or having suffered late toxicosis of pregnant women. On the basis of impaired adaptive reactions, changes in the enzymatic spectrum of blood cells and disorders of microcirculatory mechanisms, children at risk were identified. 50 of them underwent preventive therapy in the neonatal period. The follow-up indicates a lower infectious index during the first year of life in children who received preventive therapy.

Vena capitis prima and the cavernous sinus in human embryos and fetuses

2020 ◽  
Vol 229 ◽  
pp. 151467 ◽  
Author(s):  
Shogo Hayashi ◽  
Ji Hyun Kim ◽  
Kwang Ho Cho ◽  
Gen Murakami ◽  
Jörg Wilting ◽  
...  
Keyword(s):  
Cavernous Sinus ◽  
Human Embryos ◽  
Human Embryos And Fetuses

102. THE EFFECT OF INSULIN ON EMBRYONIC STEM CELL PROGENITOR CELLS IN THE MOUSE BLASTOCYST

2009 ◽  
Vol 21 (9) ◽  
pp. 21
Author(s):  
J. M. Campbell ◽  
I. Vassiliev ◽  
M. B. Nottle ◽  
M. Lane
Keyword(s):  
Progenitor Cells ◽  
Cell Fate ◽  
Culture Medium ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Cell Number ◽  
Human Embryos ◽  
Cell Stage ◽  
Stage Of Development

Human ESCs are produced from embryos donated at the mid-stage of pre-implantation development. This cryostorage reduced viability. However, it has been shown that this can be improved by the addition of growth factors to culture medium. The aim of the present study was to examine whether the addition of insulin to embryo culture medium from the 8-cell stage of development increases the number of ES cell progenitor cells in the epiblast in a mouse model. In vivo produced mouse zygotes (C57Bl6 strain) were cultured in G1 medium for 48h to the 8-cell stage, followed by culture in G2 supplemented with insulin (0, 0.17, 1.7 and 1700pM) for 68h, at 37 o C , in 5% O2, 6%CO2, 89% N2 . The number of cells in the inner cell mass (ICM) and epiblast was determined by immunohistochemical staining for Oct4 and Nanog. ICM cells express Oct4, epiblast cells express both Oct4 and Nanog. The addition of insulin at the concentrations examined did not increase the ICM. However, at 1.7pM insulin increased the number of epiblast cells (6.6±0.5 cells vs 4.1±0.5, P=0.001) in the ICM, which increased the proportion of the ICM that was epiblast (38.9±3.7% compared to 25.8±3.4% in the control P=0.01). This indicates that the increase in the epiblast is brought about by a shift in cell fate as opposed to an increase in cell division. The effect of insulin on the proportion of cells in the epiblast was investigated using inhibitors of phosphoinositide3-kinase (PI3K) (LY294002, 50µM); one of insulin's main second messengers, and p53 (pifithrin-α, 30µg/ml); a pro-apoptotic protein inactivated by PI3K. Inhibition of PI3K eliminated the increase caused by insulin (4.5±0.3 cells versus 2.2±0.3 cells, P<0.001), while inhibition of p53 increased the epiblast cell number compared to the control (7.1±0.8 and 4.1±0.7 respectively P=0.001). This study shows that insulin increases epiblast cell number through the activation of PI3K and the inhibition of p53, and may be a strategy for improving ESC isolation from human embryos.

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