Collection 10 / ІІІ

ABSTRACT The effect of growth hormone (GH) in vitro on the rate of intracellular accumulation of the non-utilizable amino acid α-aminoisobutyric acid (AIB) was studied in the intact rat diaphragm preparation. Bovine or ovine GH (25 μg/ml incubation medium) markedly stimulated the accumulation of AIB-14C by diaphragms from hypophysectomized rats, while there was no or only a very slight effect on diaphragms from normal rats. In diaphragms from rats with the pituitary gland autotransplanted to the kidney capsule GH in vitro stimulated the accumulation of AIB-14C significantly more than in diaphragms from normal rats but significantly less than in diaphragms from hypophysectomized rats. Injections of GH intramuscularly for 4 days to hypophysectomized rats made the diaphragms from these rats less sensitive or completely insensitive to GH in vitro. These results indicate strongly that the relative insensitivity to GH in vitro of diaphragms from normal rats is due to the fact that the muscle tissues from these rats has been exposed to the endogenously secreted GH. The results show that GH can influence the accumulation of AIB-14C in the isolated rat diaphragm in two different ways giving an acute or »stimulatory« effect and a late or »inhibitory« effect, and that it seems to be a time-relationship between these two effects of the hormone.

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EFFECT OF ERGOCORNINE METHANESULPHONATE ON THE LUTEOTROPHIC ACTIVITY OF THE ECTOPIC MOUSE TROPHOBLAST

Acta Endocrinologica ◽

10.1530/acta.0.0590442 ◽

1968 ◽

Vol 59 (3) ◽

pp. 442-446 ◽

Cited By ~ 5

Author(s):

G. H. Zeilmaker

Keyword(s):

Direct Effect ◽

Oestrous Cycle ◽

Kidney Capsule ◽

Blastocyst Implantation

ABSTRACT Virgin mice received 2 blastocysts under the kidney capsule. Following an oestrous cycle which occurred 1–4 days after the operation, nearly all the animals became pseudopregnant. It was found that pseudopregnancy induced by ectopic trophoblast could not be terminated by ergocornine administration. In contrast, this drug was effective in interrupting pseudopregnancy induced by sterile mating. The results confirm previous findings a) that placental luteotrophic hormone starts acting soon after blastocyst implantation and b) that ergocornine exerts no direct effect on the ovary.

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Pancreatic islet transplantation under the kidney capsule of mice: model of refinement for molecular and ex-vivo graft analysis

Laboratory Animals ◽

10.1177/00236772211004051 ◽

2021 ◽

pp. 002367722110040

Author(s):

Julien Thévenet ◽

Valery Gmyr ◽

Nathalie Delalleau ◽

François Pattou ◽

Julie Kerr-Conte

Keyword(s):

Islet Transplantation ◽

Pain Treatment ◽

Ex Vivo ◽

Well Being ◽

Diabetic Patients ◽

Mice Model ◽

Pancreatic Islet Transplantation ◽

Post Transplantation ◽

Kidney Capsule

Diabetes cell therapy by human islet transplantation can restore an endogenous insulin secretion and normal glycaemic control in type 1 diabetic patients for as long as 10 years post transplantation. Before transplantation, each clinical islet preparation undergoes extensive in-vitro and in-vivo quality controls. The in-vivo quality control assay consists of transplanting human islets under the kidney capsule of immunocompromised mice. Currently, it is considered the best predictive factor to qualify clinical transplant efficiency. This chimeric model offers a wide area of study since it combines the possibility of producing not only quantitative but also a maximum of qualitative data. Today’s technological advances allow us to obtain more accurate and stronger data from the animals used in research while ensuring their comfort and well-being throughout the protocol, including cage enrichment and pain treatment during and after surgery. As demonstrated in this valuable model, we are able to generate more usable results (Refine), while reducing the number of animals used (Reduce), by focusing on the development of ex-vivo analysis techniques (Replace), which clearly highlights the Burch and Russell 3Rs concept.

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Serotonin stimulates GH secretion through a direct pituitary action: studies in hypophysectomized autografted animals and in perifused pituitaries

Acta Endocrinologica ◽

10.1530/acta.0.1130317 ◽

1986 ◽

Vol 113 (3) ◽

pp. 317-322 ◽

Cited By ~ 12

Author(s):

F. López ◽

D. Gónzalez ◽

E. Aguilar

Keyword(s):

Plasma Volume ◽

Direct Action ◽

Experimental Models ◽

Male Rats ◽

Blood Samples ◽

Kidney Capsule ◽

Gh Secretion ◽

Significant Release ◽

The Right ◽

Dose Dependent

Abstract. To analyze a possible direct action of serotonin (5-hydroxytryptamine) at pituitary level in GH secretion, two experimental models were used: hypophysectomized autografted rats and perifused pituitaries. Adult male rats were hypophysectomized and their own pituitaries placed under the right kidney capsule. Ten days later an intra-atrial cannula was inserted. The next day, blood samples were obtained before and every 10 min during a 2 h period after the injection of saline or 5-hydroxytryptamin (1 or 2 mg/kg iv). Plasma volume was replaced with saline. Both doses of 5-hydroxytryptamine elicit a strong release of GH, the effect being dose-dependent. In pituitaries perifused with 5-hydroxytryptamine (100 μm during 115 min or 1, 10 and 100 μm during 15 min), a significant release of GH was also observed. These results suggested that 5-hydroxytryptamine may stimulate GH secretion through a direct pituitary action.

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Persistence of lpr/lpr-derived lgG2a secreting B cells in normal → lpr/lpr adult radiation chimeras or lpr/lpr → normal kidney capsule chimeras

Cellular Immunology ◽

10.1016/0008-8749(92)90293-x ◽

1992 ◽

Vol 142 (2) ◽

pp. 313-325 ◽

Cited By ~ 1

Author(s):

Roseann M. Glaser ◽

Carol A. Mahon ◽

Ann Marshak-Rothstein

Keyword(s):

B Cells ◽

Normal Kidney ◽

Kidney Capsule ◽

Radiation Chimeras

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Investigation on the origin of the definitive endoderm in the rat embryo

Development ◽

10.1242/dev.32.2.445 ◽

1974 ◽

Vol 32 (2) ◽

pp. 445-459

Author(s):

B. Levak-Švajger ◽

A. Švajger

Keyword(s):

Primitive Streak ◽

Definitive Endoderm ◽

Rat Embryo ◽

Chick Blastoderm ◽

Germ Layers ◽

Kidney Capsule ◽

Rat Embryos ◽

Endodermal Cells ◽

Derivatives Of

Single germ layers (or combinations of two of them) were isolated from the primitive streak and the head-fold stage rat embryos and grown for 15 days under the kidney capsule of syngeneic adult animals. The resulting teratomas were examined histologically for the presence of mature tissues, with special emphasis on derivatives of the primitive gut. Ectoderm isolated together with the initial mesodermal wings at the primitive streak stage gave rise to tissue derivatives of all three definitive germ layers. Derivatives of the primitive gut were regularly present in these grafts. At the head-fold stage, isolated ectoderm (including the region of the primitive streak) differentiated into ectodermal and mesodermal derivatives only. Endoderm isolated at the primitive streak stage did not develop when grafted and was always completely resorbed. At the head-fold stage, however, definitive endoderm differentiated into derivatives of the primitive gut if grafted together with adjacent mesoderm. These findings indirectly suggest the migration of prospective endodermal cells from the primitive ectoderm, and therefore a general analogy with the course of events during gastrulation in the chick blastoderm.

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