scholarly journals Genetic Diversity of Cercospora arachidicola Associated with Peanut Early Leaf Spot in Shandong Province of China

2021 ◽  
Vol 25 (03) ◽  
pp. 645-650
Author(s):  
Yun Geng
Keyword(s):  
Genetic Diversity ◽  
Leaf Spot ◽  
Management Practices ◽  
Genetic Relatedness ◽  
Issr Markers ◽  
Shandong Province ◽  
Cercospora Arachidicola ◽  
Inter Simple Sequence Repeats ◽  
Cultivated Peanut ◽  
Generic Groups

Early leaf spot caused by Cercospora arachidicola is the major destructive foliar disease of cultivated peanut in Shandong province of China. Understanding the genetic variability of this pathogen is crucial for evolutionary comprehension and breeding strategies. The genetic diversity of C. arachidicola isolates obtained from different peanut growing regions of Shandong province were assessed using inter-simple sequence repeats (ISSR) markers. Thirteen ISSR primers generating polymorphic, clearly discernible and reproducible patterns were screened out for further analysis. A total of 113 distinct bands were amplified, of which 85.8% were polymorphic, suggesting high values of polymorphism among the isolates. Cluster analysis using UPGMA indicated that all the isolates tested were separated into three distinct generic groups. The genetic relatedness of C. arachidicola isolates roughly coincided with geographical origin. Analysis of molecular variance (AMOVA) revealed that the observed genetic variation mainly existed within populations, and the variation among populations was weak. This study characterized the genetic diversity of C. arachidicola isolates for the first time, which will provide necessary genetic information for effective management practices. © 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers © 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers© 2021 Friends Science Publishers©

scholarly journals Genetic Diversity of Selected Mangifera Species Revealed by Inter Simple Sequence Repeats Markers

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Zulhairil Ariffin ◽  
Muhammad Shafie Md Sah ◽  
Salma Idris ◽  
Nuradni Hashim
Keyword(s):  
Genetic Diversity ◽  
Genetic Similarity ◽  
Genetic Relatedness ◽  
Mangifera Indica ◽  
Issr Markers ◽  
Shannon Index ◽  
Inter Simple Sequence Repeats ◽  
High Degree ◽  
Simple Sequence ◽  
Dice Index

ISSR markers were employed to reveal genetic diversity and genetic relatedness among 28 Mangifera accessions collected from Yan (Kedah), Bukit Gantang (Perak), Sibuti (Sarawak), and Papar (Sabah). A total of 198 markers were generated using nine anchored primers and one nonanchored primer. Genetic variation among the 28 accessions of Mangifera species including wild relatives, landraces, and clonal varieties is high, with an average degree of polymorphism of 98% and mean Shannon index, H0=7.50. Analysis on 18 Mangifera indica accessions also showed high degree of polymorphism of 99% and mean Shannon index, H0=5.74. Dice index of genetic similarity ranged from 0.0938 to 0.8046 among the Mangifera species. The dendrogram showed that the Mangifera species were grouped into three main divergent clusters. Cluster 1 comprised 14 accessions from Kedah and Perak. Cluster II and cluster III comprised 14 accessions from Sarawak and Sabah. Meanwhile, the Dice index of genetic similarity for 18 accessions of Mangifera indica ranged from 0.2588 to 0.7742. The dendrogram also showed the 18 accessions of Mangifera indica were grouped into three main clusters. Cluster I comprised 10 landraces of Mangifera indica from Kedah. Cluster II comprised 7 landraces of Mangifera indica followed by Chokanan to form Cluster III.

scholarly journals Molecular analysis of genetic diversity in population of Silybum marianum (L.) Gaertn in Egypt

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Marwa Hamouda
Keyword(s):  
Genetic Diversity ◽  
Electrophoretic Pattern ◽  
Issr Markers ◽  
Silybum Marianum ◽  
Inter Simple Sequence Repeats ◽  
Sds Page ◽  
Dna Pattern ◽  
Pharmaceutical Properties ◽  
High Level ◽  
Rapd And Issr

Abstract Background Silybum marianum L. Gaertn is a medicinal plant of unique pharmaceutical properties in the treatment of liver disorders and diabetic nephropathy. Biochemical (SDS-PAGE) and molecular markers such as randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) technologies were used in this work to detect genetic diversity of 14 collections of Silybum marianum population in Egypt. Results The electrophoretic pattern of seed protein gave different molecular weight bands, ranging from 24 to 111 KDa with the presence of unique bands. RAPD results revealed a high level of polymorphism (73.2%) using 12 RAPD primers, but only eight of them gave reproducible polymorphic DNA pattern. Sixteen primers were used in the ISSR method; only ten of them yielded clearly identifiable bands. The percentage of polymorphism is about 80% of the studied samples. Conclusion The obtained data confirmed that SDS-protein, RAPD, and ISSR markers are important tools for genetic analysis for Silybum marianum and recommended to give accurate results.

Combination of Early Maturity and Leaf Spot Tolerance Within an Advanced Georgia Peanut Breeding Line1

1995 ◽  
Vol 22 (2) ◽  
pp. 106-108 ◽  
Author(s):  
W. D. Branch ◽  
A. K. Culbreath
Keyword(s):  
Leaf Spot ◽  
Genetic Resistance ◽  
Field Tests ◽  
Cercospora Arachidicola ◽  
Early Maturity ◽  
The Past ◽  
Leaf Spots ◽  
Arachis Hypogaea L ◽  
Cultivated Peanut ◽  
Efficient Alternative

Abstract In the past, genetic resistance to both early and late leaf spots [Cercospora arachidicola Hori and Cercosporidiumpersonatum (Berk. & Curt.) Deighton] has been found to be negatively or inversely correlated with early maturity in the cultivated peanut (Arachis hypogaea L.). For example, the late leaf spot resistant cultivar Southern Runner is approximately 2 wk later in maturity than the susceptible Florunner cultivar. Recently, an advanced runner-type breeding line (GA T-2844) has been developed by the Georgia peanut breeding program which combines early maturity and leaf spot tolerance. For the past 3 yr (1991–1993), GA T-2844 has been evaluated in replicated field tests without fungicides. Results show that GA T-2844 has on the average >30% yield advantage and a 30-d earlier maturity than Southern Runner. Leaf spot ratings also showed GA T-2844 to be intermediate between Southern Runner and Florunner. Such a combination of early maturity and leaf spot tolerance could significantly enhance U.S. peanut production by providing an environmentally safer and efficient alternative to costly pesticides not previously available among runner-type cultivars.

scholarly journals Evaluation of genetic diversity of cultivated tea clones (Camellia sinensis (L.) Kuntze) in the eastern black sea coast by inter-simple sequence repeats (ISSRS)

Genetika ◽  
2016 ◽  
Vol 48 (1) ◽  
pp. 87-96 ◽  
Author(s):  
Fatih Beris ◽  
Necla Pehlivan ◽  
Melike Kac ◽  
Ayhan Haznedar ◽  
Fatih Coşkun ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Black Sea ◽  
Issr Markers ◽  
Base Pairs ◽  
Inter Simple Sequence Repeats ◽  
Black Sea Coast ◽  
Geographical Position ◽  
Tea Plants ◽  
Relationship Of ◽  
Sea Coast

Tea is the most globally consumed drink after spring water and an important breeding plant with high economical value in Turkey. In half a century, various kinds of tea cultivars have been bred in Turkey to improve the quality and yield of tea plants. Since tea reproduces sexually, tea fields vary in quality. Thus, determining the genetic diversity and relationship of the plants to support breeding and cultivation is important. In this study we aimed to determine the genetic diversity of tea cultivars breeding in the Eastern Black Sea coast of Turkey and the genetic relationship between them, to verify whether the qualitative morphological designations of the clones are genetically true by the ISSR markers. Herein, the genetic diversity and relationships of 18 Turkish tea cultivars were determined using 15 ISSR markers with sizes ranging from 250 to 3000 base pairs. The similarity indices among these cultivars were between 0.456 and 0.743. Based on cluster analysis using UPGMA, some of tea cultivars originating from the same geographical position were found to be clustered closely. Our data provide valuable information and a useful basis to assist selection and cloning experiments of tea cultivars and also help farmers to find elite parental clones for tea breeding in the Eastern Black Sea coast of Turkey.

Genetic diversity analysis of sweet potato [Ipomoea batatas (L.) Lam.] germplasms through RAPD and ISSR markers

2021 ◽  
Vol 50 (1) ◽  
pp. 119-129
Author(s):  
PN Narasimha Murthy ◽  
NB Patel ◽  
Chintan Kapadia ◽  
KD Desai ◽  
G Koteswara Rao
Keyword(s):  
Genetic Diversity ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Genetic Relatedness ◽  
Issr Markers ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Polymorphic Loci ◽  
Improvement Programme ◽  
Rapd And Issr

Sweet potato [Ipomoea batatas (L.) Lam.] germplasms are unique as they are heterozygous, vegitatively as well as sexually propagated and polyploid in nature. Forty-four germplasms from local farmer’s field and from various centres of India were determined using 15 RAPD and 15 ISSR markers to evaluate their genetic relatedness. Average polymorphisms for RAPD and ISSR markers were found to be 86.72 and 81.64%, respectively. The OPM series primer produced 145 total bands with 138 polymorphic loci and ISSR markers gave 116 total bands with 101 polymorphic loci. The clusters were made using the UPGMA method using both the RAPD and ISSR data and group diverse yet similar germplasm in two different clusters. This gives ample information to use them in further improvement programme.

Genetic relatedness among cultivated and wild mulberry (Moraceae: Morus) as revealed by inter-simple sequence repeat analysis in China

2006 ◽  
Vol 86 (1) ◽  
pp. 251-257 ◽  
Author(s):  
Zhao Weiguo ◽  
Zhou Zhihua ◽  
Miao Xuexia ◽  
Wang Sibao ◽  
Zhang Lin ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Genetic Relatedness ◽  
Genetic Relationships ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Group Method ◽  
Sequence Repeat ◽  
Pair Group ◽  
Simple Sequence

The genetic diversity of 27 mulberry (Morus spp.) genotypes mainly from China was investigated using inter-simple sequence repeat (ISSR) markers to assist in addressing breeding objectives and conserving existing genetic resources. Of the 22 primers screened, 15 produced highly reproducible ISSR bands. Using these 15 primers, 138 discernible DNA fragments were generated with 126 (91.3%) being polymorphic, indicating considerable genetic variation among the mulberry genotypes studied. Genetic similarity ranged from 0.6014 between Yu 2 and Yu 711 to 0.9493 between Cuizhisang and Dejiang 10. The phenetic dendrogram based on ISSR data generated by the unweighed pair group method with arithmetical averages (UPGMA) method grouped the 27 accessions into two major clusters: cluster I, cultivated mulberry species (M. multicaulis Perr., M. alba Linn., M. atropurpurea oxb., M. bombycis Kiodz., M. australis Poir., M. rotundiloba Kiodz., M. alba var. pendula Dipp., M. alba var. macrophylla Loud., and M. alba var. venose Delile.); and cluster II, wild mulberry species (M. cathayana Hemsl., M. laevigata Wall., M. wittiorum Hand-Mazz., M. nigra Linn., and M. mongolica Schneid.). Our molecular analyses agree with the existing morphological classification of Morus and clarify the genetic relationships among mulberry species. Key words: Morus L., genetic diversity, inter-simple sequence repeat, relatedness

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