scholarly journals Histologi, Histomorfometri, dan Histokimia Usus Ayam Buras (Gallus gallus domesticus) Selama Periode Sebelum dan Setelah Menetas

2021 ◽  
Vol 21 (2) ◽  
pp. 128-140
Author(s):  
Bagus Dwijayanti ◽  
Erdiansyah Rahmi ◽  
Ummu Balqis ◽  
Fitriani Fitriani ◽  
Dian Masyitha ◽  
...  
Keyword(s):  
Incubation Period ◽  
Periodic Acid ◽  
Gallus Gallus ◽  
Gallus Domesticus ◽  
Muscularis Mucosa ◽  
Gallus Gallus Domesticus ◽  
Periodic Acid Schiff ◽  
Chicken Intestine ◽  
Native Chicken ◽  
Significant Difference

ABSTRACT. Usus adalah salah satu organ pencernaan yang berperan penting pada proses absorpsi nutrisi sebagai sumber energi. Penelitian ini bertujuan mengetahui histologi, histomorfometri dan histokimia sebaran glikogen pada usus ayam buras (Gallus gallus domesticus) sebelum dan setelah menetas menggunakan pewarnaan Haematoksilin-Eosin (HE) dan pewarnaan Periodic Acid Schiff (PAS). Sampel penelitian terdiri atas empat kelompok tingkat umur berbeda, setiap kelompok berjumlah enam usus ayam. Hasil penelitian menunjukkan perbedaan nyata (P˂0,05) morfometri usus ayam buras secara makroskopis. Secara histologis pada setiap perkembangan sudah terlihat lapisan usus yang terdiri atas tunika mukosa, submukosa, muskularis, dan serosa. Pada tunika mukosa, didapati tiga lamina yaitu lamina epitelia, propria, dan muskularis mukosa yang mulai teramati pada masa inkubasi hari ke-14. Lamina epitelia berupa sel epitel silindris selapis dengan mikrovili, lamina propria berupa jaringan ikat, dan lamina muskularis mukosa berupa berkas otot polos. Pada tunika mukosa dijumpai adanya sel Goblet dan kripta Liberkuhn yang teramati pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7. Hasil histomorfometri ketebalan lapisan usus, panjang vili, lebar vili, dan diameter lumen usus ayam buras pada masa inkubasi dan setelah menetas menunjukkan perbedaan nyata (P˂0,05). Temuan reaksi positif kuat terhadap pewarnaan PAS dijumpai pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7 dan positif lemah pada masa inkubasi hari ke-14. Dapat disimpulkan bahwa terdapat korelasi antara perkembangan usus ayam secara makroskopis, histomorfometri ketebalan tunika dan vili, serta sebaran reaksi positif kuat terhadap pewarnaan PAS pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7.  (Histological, histomorphometrical, and histochemical of intestine on native chicken (Gallus gallus domesticus) during pre and posthatch period) ABSTRAK. The intestines are one of the digestive organ that play an important role in the nutrient absorption process as an energy source. The aim of this study was to determine the histomorphometric and histochemical of glycogen distribution in the intestines of native chickens pre and post hatching periods using Haematoxylin-Eosin (HE) staining and Periodic Acid Schiff (PAS) staining. The samples of this study was consisted of four different age groups, each group consisting of six chicken intestines. The results showed a significant difference (P˂0.05) on macroscopic morphometry of the native chicken’s intestine. Histologically in each group has been found the intestines consisting of mucosa, submucous, muscularis, and serous tunic. In the mucosal tunic, there were three layers; ephitelial, propria, and muscularial mucous can be observed during the 14th day of incubation period. Ephitelial layer in the form of cylindrical epithelial cells with microvilli, proprial layer in the form of connective tissue, and layer of the muscularis mucosa form smooth muscle thread. In the mucosal tunic, there was Goblet cells and Liberkuhn crypts that can be observed during the 20th day incubation period and 7th day post hatch. Histomorphometry results of intestinal lining thickness, villi length, villi width, and lumen diameter of native chicken intestine during incubation and post hatching showed significant differences (P˂0.05). The findings of a strong positive reaction to the staining of PAS were found on the incubation period of the 20th day and post hatching the 7th day and positively weak on the incubation period of the 14th day. It can be concluded that there is a correlation between macroscopic development of chicken intestine, histomorphometric of tunica and villous thickness, as well as the distribution of strong positive reactions to the staining of PAS during the incubation period of the 20th day and post hatching the 7th day.

scholarly journals Histologi, Histomorfometri, dan Histokimia Hati Ayam Buras (Gallus gallus domesticus) Selama Periode Sebelum dan Setelah Menetas

2020 ◽  
Vol 20 (2) ◽  
Author(s):  
Siswandy Siswandy ◽  
Erdiansyah Rahmi ◽  
Dian Masyitha ◽  
Fitriani Fitriani ◽  
Fadli A. Gani ◽  
...  
Keyword(s):  
Gallus Gallus ◽  
Gallus Domesticus ◽  
Chicken Liver ◽  
Central Vein ◽  
Gallus Gallus Domesticus ◽  
Liver Growth ◽  
Native Chicken ◽  
Significant Difference ◽  
Vein Diameter ◽  
Native Chickens

ABSTRAK. Hati adalah kelenjar terbesar yang terdapat di dalam tubuh dan dapat dianggap sebagai organ pusat dalam pemeliharaan suplai energi. Penelitian ini bertujuan untuk mengetahui histologi, histomorfometri, dan histokimia sebaran glikogen pada hati ayam buras sebelum dan sesudah menetas. Sampel penelitian dibagi menjadi empat kelompok tingkat umur berbeda dan masing-masing kelompok berjumlah enam hati ayam. Hasil penelitian menunjukkan perbedaan nyata (P0,05) pertumbuhan hati ayam buras secara makroskopis. Pada setiap perkembangan sudah terdapat hepatosit berbentuk poligon yang memiliki inti sel bulat di tengah hepatosit, terdapat juga vena central yang dilapisi sel endotel pipih selapis serta didapati sinusoid yang berada diantara hepatosit, dan juga ditemukan Kupffer’s cell hanya pada hati ayam setelah menetas hari ke-7. Hasil pengukuran diameter vena central hati ayam buras hari ke-7, hari ke-14, hari ke- 20 sebelum menetas dan hari ke-7 setelah menetas berturut-turut adalah 17,70 ± 1,16 µm; 60,32 ± 2,22 µm; 49,316 ± 1,93 µm; dan 129,11 ± 7,43 µm. Hasil histokimia sebaran glikogen pada hati ayam buras hari ke-14, hari ke- 20 sebelum menetas dan hari ke-7 setelah menetas berturut-turut adalah 1,44 ± 0,93; 2,27 ± 0,74; dan 2,77 ± 0,15. Dapat disimpulkan, bahwa diameter vena central dan sebaran glikogen meningkat seiring pertumbuhan ayam buras. (Histological, histomorphometrical, and histochemical of liver on native chicken (Gallus gallus domesticus) during pre and posthatch period) ABSTRACT. The liver is the largest gland in the body and considered as a central organ in maintaining energy supplies. The study aimed to determine the histologic, histomorphometric and histochemical of glycogen distribution in the liver of native chicken during pre and posthatching period.. The study sample were divided into four different age groups and each group consisted of six chicken livers. The results showed a significant difference (P0,05) in native chicken liver growth macroscopically. In every development there are already polygonal hepatocytes that have a round cell nucleus in the middle of the hepatocytes, there is also a central vein that is coated with a layer of flat endothelial cells and also sinusoids that are between the hepatocytes, and kupffer's cells also found in chicken liver 7th day posthatching. The results measurements of the central vein diameter in liver native chickens on the 7th, 14th, 20th pre hatching period and 7th post hatching were 17.70 ± 1.16 µm; 60.32 ± 2.22 µm; 49.32 ± 1.93 µm; and 129.11 ± 7,43 µm; The measure results of the glycogen distribution in liver native chickens on the 14th, 20th pre hatching period and 7th post hatching were 1,44 ± 0,93; 2,27 ± 0,74; and 2,77 ± 0,15. It can be concluded, that central vein diameter and glycogen distribution were increased by growth of native chickens.

scholarly journals Effect of Frozen Storage on the Water- Holding Capacity and pH of Broiler Chicken cut-up Parts (Gallus gallus domesticus)

2020 ◽  
pp. 8-15
Author(s):  
Arjun Ghimire ◽  
Pawan Parajuli
Keyword(s):  
Broiler Chicken ◽  
Frozen Storage ◽  
Gallus Gallus ◽  
Gallus Domesticus ◽  
Cooking Loss ◽  
Water Holding Capacity ◽  
Drip Loss ◽  
Gallus Gallus Domesticus ◽  
Significant Difference ◽  
Water Holding

Broiler chickens (Gallus gallus domesticus) were collected from local chicken suppliers of Dharan (26.8065° N, 87.2846° E), Nepal, and slaughtered to prepare the cut-up parts (breast, drumstick, dorso, wings, and thigh). They were vacuum packed in polythene bags and frozen stored (-21.5 ±3.5ºC) for 45 days to study the effect of frozen storage on the water holding capacity (WHC) of meat. Cooking loss, drip loss, and pH changes were analyzed at 5 days intervals during storage and the data were analyzed using Genstat® v 12.1.The cut-up parts showed a significant change (p<0.05) in pH, cooking loss, and drip loss during 45 days of frozen storage. The pH decreased while the drip loss and the cooking loss increased gradually upon storage. The pH was found to be varied among the cut-up parts with a minimum pH scale of 5.25 for wings, followed by 5.3 for thigh, at the end. The highest drip loss of 6.11% was observed in wings after 5 days of storage which ended up to 8.97% after 45 days of frozen storage while drumstick and thigh samples showed significantly lower drip loss of 6.02%, and 5.43% respectively. The change in cooking loss showed a similar pattern as that of drip loss with a significantly higher value of 37.36% in the breast while it showed no significant difference in drumstick, dorso, and thigh meat. Thus, the study reveals that freezing of broiler chicken meats for a prolonged period adversely affects the WHC of meat

scholarly journals APLIKASI TEKNOLOGI DIELECTRIC BARRIER DISCHARGE-UV PLASMA TERHADAP SIFAT FISIK DAN KIMIA TELUR AYAM (Gallus gallus domesticus)

BUANA SAINS ◽  
2018 ◽  
Vol 18 (1) ◽  
pp. 85
Author(s):  
Endrika Widyastuti ◽  
Aldila Daydeva
Keyword(s):  
Dielectric Barrier Discharge ◽  
Barrier Discharge ◽  
Chemical Properties ◽  
Gallus Gallus ◽  
Gallus Domesticus ◽  
Gallus Gallus Domesticus ◽  
Dielectric Barrier ◽  
Significant Difference ◽  
Physical And Chemical Quality ◽  
Haugh Unit

Eggs are food that contains high nutrition, however egg products susceptible damage so preservation process is required. Dielectric Barrier Discharge (DBD) UV Plasma has proven very effective as an alternative technology to preserve fresh egg by inactivation bacteria and prevent contamination in a fresh egg. the aim of this study is to evaluated effect of Dielectric Barrier Discharge (DBD) UV Plasma on physical and chemical quality of chicken eggs (Gallus gallus domesticus). The fresh eggs were stored at room temperature and assessed for their Physical (Haugh unit and yolk index) and chemical properties (yolk pH, albumen pH, and egg nutrition) on 0, 5, 7, 11, and 14 days. Analysis of variance (ANOVA) was used to analyze significant difference among the samples. The result shows that DBD UV plasma can maintain haugh unit, yolk index, yolk pH, and albumen pH than control. The eggs treated with DBD UV recorded have no significant difference for egg nutrition.

scholarly journals Consistency of dark skeletal muscles in Thai native black-bone chickens (Gallus gallus domesticus)

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10728 ◽  
Author(s):  
Wannapimol Kriangwanich ◽  
Promporn Piboon ◽  
Wirakorn Sakorn ◽  
Kittisak Buddhachat ◽  
Varankpicha Kochagul ◽  
...  
Keyword(s):  
Skeletal Muscles ◽  
Gallus Gallus ◽  
Gallus Domesticus ◽  
Melanin Pigment ◽  
Whole Body ◽  
Gallus Gallus Domesticus ◽  
Color Chart ◽  
Flexor Carpi Ulnaris ◽  
Significant Difference ◽  
Body Organ

Black-bone chickens (Gallus gallus domesticus) have become economically valuable, particularly in Southeast Asia as a consequence of popular traditional Chinese medical practices. Chickens with whole body organ darkness are considered to have higher value and are, therefore, more often requested. This research study aimed to investigate the darkness in 34 skeletal muscles of 10 Thai black-bone chickens (five males and five females). The evaluation of muscle darkness was done on two levels: (i) a color chart was employed at the macroanatomical level and (ii) by using melanin pigment to evaluate the structure at the microanatomy level. The results revealed that the accumulation of melanin pigment in the muscle tissue was observed in the endomysium, perimysium and epimysium. With respect to the results of the color chart test, iliotibialis lateralis pars preacetabularis, gastrocnemius, fibularis longus and puboischiofemoralis pars medialis showed the highest degree of darkness, while serratus profundus, pectoralis, iliotibialis cranialis, flexor cruris lateralis, and flexor cruris medialis appeared to be the least dark. In addition, we found that the highest and lowest amounts of melanin pigment was noted in the flexor carpi ulnaris and pectoralis (p < 0.05), respectively; however, there was no significant difference (p > 0.05) observed between the sexes. These results reveal that the 34 specified muscles of black-bone chickens showed uneven distribution of darkness due to the differing accumulations of melanin pigments of each muscle.This information may provide background knowledge for a better understanding of melanin accumulation and lead to breeding improvements in Thai black-bone chickens.

scholarly journals Prevalence of Intestinal Helminth Parasites of Chicken (Gallus gallus domesticus Linnaeus, 1758) in Lalitpur District, Nepal

2018 ◽  
Vol 32 (2) ◽  
pp. 19-28
Author(s):  
Janak Raj Subedi ◽  
Tasneem Mujahid ◽  
Bijay Cheetri
Keyword(s):  
Prevalence Rate ◽  
Potassium Dichromate ◽  
Gallus Gallus ◽  
Gallus Domesticus ◽  
Intestinal Helminth ◽  
Helminth Parasites ◽  
Gallus Gallus Domesticus ◽  
Free Range ◽  
Significant Difference ◽  
Unidentified Species

This study was aimed to determine the prevalence of intestinal helminth parasites of local chicken (Gallus gallus domesticus) from Lalitpur district, Nepal. It was carried out from February 2014 to March 2015. A total of 125 samples (93 alimentary canals of freshly killed chicken and 32 stool samples from ground and fowl runs) were collected. Out of 93 alimentary canals 17 were collected from free range chicken and 76 were from slaughtered house i.e. from poultry farm chicken. The faecal samples were collected in the sterile vials containing 2.5 percent potassium dichromate. Depending upon the convenience, post mortem examination method, differential floatation method and direct smear method were used for the examination of samples. The present study showed only 40 percent of all the poultry examined as infected. Overall 5 species of nematodes, 1 species of cestode and 4 unidentified species were found to be prevalent in chicken from Lalitpur district. The highest prevalence rate was found with Heterakis gallinarum (22.4%) followed by Capillaria species (16%), Ascaridia galli (10.4%), unidentified species (4.8%) and Raillietina tetragona (4%). Statistically there was a significant difference in the prevalence of helminth species (χ2=33.83; p< 0.05; α=1). Likewise, there was significant difference in the prevalence rate of helminth parasites in free range chicken and poultry chicken (χ2=22.055; p< 0.05; α=1).

Sign in / Sign up

Export Citation Format

Share Document