scholarly journals Regulatory T cells (CD4+CD25+FOXP3+) in lupus nephritis

2018 ◽  
Vol 7 (1) ◽  
pp. 219
Author(s):  
Mohd Khairul Mohd Kamil ◽  
Rozita Mohd ◽  
Rizna Abdul Cader ◽  
Azlin Ithnin ◽  
Shamsul Azhar Mohd Shah
Keyword(s):  
T Cells ◽  
Lupus Nephritis ◽  
Regulatory T Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Activity Index ◽  
Demographic Data ◽  
Peripheral Blood Mononuclear ◽  
Cross Sectional ◽  
Healthy Control

Background: Systemic lupus erythromatosus (SLE) is an autoimmune disease with 20–65% of patients developing lupus nephritis (LN). Studies have reported 10% of LN patients will end up with end stage renal disease and their mortality rate is higher compared to patients without LN. Abnormality of regulatory T cells (Tregs) level is thought to be a potential factor for this LN development. The aim of study was to evaluate the percentage of Tregs in LN patients.Methods: This was a comparative cross sectional study involving LN patients and age and gender matched controls with a 2:1 ratio. The patients were grouped into active and inactive LN based on their lupus activity index; complement levels, ANA, dsDNA antibodies, ESR, SLE Disease Activity Index (SLEDAI2K) score and also urine PCI (uPCI>0.05 for active group). Disease history, demographic data, routine blood test, peripheral blood for differentials count were taken and recorded. Peripheral blood mononuclear cells were stained with CD4, CD25 and Foxp3 antibodies and percentage of Tregs was analysed using BD fluorescence-activated cell sorting (FACS) cytometer. We compared demographic and laboratory parameters between healthy controls and LN patients as well as active and inactive LN patients.Results: A total of 34 LN patients (32 females, 2 males) were recruited. Their mean age and disease duration were 37.97±11.14 years and 110.95±65.07 months respectively.  Thirteen matched controls with mean age 35.23±7.89 years were enrolled. There was no demographic difference between 2 groups of LN patients. Tregs were significantly lower in active LN compared to inactive LN and healthy control (0.44±0.37% vs. 1.89±0.46% vs. 3.12±0.56% of the CD4+, P<0.001). C3 and C4 complement fragments were significantly reduced in patients with active disease (C3; 50.92±28.43 vs. 76.31±25.63, P=0.011) and (C4; 11.17±8.41 vs. 16.70±6.50 P=0.044). Proteinuria was significantly higher while serum albumin levels were significantly lower in active patients compared to inactive patients and healthy control (urine PCI; 0.25(0.15-0.3) vs. 0.03(0.01-0.05) vs. 0.01, P<0.001) and (albumin; 29.89±6.87 vs. 36.87±3.58 vs. 40.62±1.89mmol/L, P<0.001). We found positive inversely correlation between Tregs with SLEDAI2K (r = -0.572, P=0.011) and proteinuria (r = -0.451, P=0.007).Conclusions: Tregs, C3 and C4 complements, and albumin were significantly lower while proteinuria was significantly higher in active LN. There was positive inversely correlation between the percentage of Tregs with SLEDAI2K score and proteinuria.

scholarly journals Adiponectin/AdipoR1 Axis Promotes IL-10 Release by Human Regulatory T Cells

2021 ◽  
Vol 12 ◽  
Author(s):  
Patricia Ramos-Ramírez ◽  
Carina Malmhäll ◽  
Omar Tliba ◽  
Madeleine Rådinger ◽  
Apostolos Bossios
Keyword(s):  
Flow Cytometry ◽  
T Cells ◽  
Regulatory T Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Foxp3 Expression ◽  
P38 Map Kinase ◽  
Adiponectin Receptor ◽  
Peripheral Blood Mononuclear ◽  
Cytokine Milieu

BackgroundAdiponectin is an important immunomodulatory mediator in inflammatory conditions. While we previously showed that adiponectin receptor 1 (AdipoR1) is expressed in murine regulatory T cells (Tregs), its expression in human Tregs remain unknown. Here, we examined the expression of AdipoR1 in human Tregs and whether its ligand, globular adiponectin (gAd) affects the Treg ability to secrete IL-10 and the role of Type 2 (T2) inflammation in such process.MethodsHuman Tregs from peripheral blood were analyzed by flow cytometry for AdipoR1, Helios and IL-10 expression. CD4+ T cells enriched from peripheral blood mononuclear cells (PBMCs) were cultured in the presence or the absence of gAd or the chemical adiponectin receptor agonist, AdipoRon, or in a T2 cytokine milieu. Flow cytometry was then used to assess intracellular IL-10, IL-10 secreting cells, FOXP3 and Helios expression, and phosphorylated p38 MAP kinase (MAPK). IL-10 levels in CD4+ T cell supernatants were quantified by ELISA.ResultsWe found that a subset of human Tregs expressed AdipoR1. Importantly, more Helios- cells expressed AdipoR1 than Helios+ cells. Likewise, there was a higher frequency of IL-10+ cells within Helios- AdipoR1+ Tregs compared to Helios+ AdipoR1+ Tregs. In contrast, the IL-10 mean fluorescence intensity (MFI) was higher in Helios+ AdipoR1+ Tregs compared to Helios-AdipoR1+ Tregs. When human CD4+ T cells were treated with gAd or AdipoRon, a significant increase in IL-10 secretion, FOXP3 expression, and p38 MAPK phosphorylation was observed in Helios- AdipoR1+ Tregs. Interestingly, gAd under T2 cytokine milieu significantly increased the intracellular levels of IL-10, mainly in Helios+ AdipoR1+ Tregs, and IL-10 levels in supernatants of CD4+ T cells.ConclusionsCollectively, our findings suggest that adiponectin/AdipoR1 axis promotes IL-10 release by Tregs, mainly in Helios- Tregs, and the effect was amplified by T2 inflammation in Helios+ Tregs.

scholarly journals Peripheral blood mononuclear cells and regulatory T cells in acute viral hepatitis.

Gut ◽  
1985 ◽  
Vol 26 (7) ◽  
pp. 739-744 ◽  
Author(s):  
V Barnaba ◽  
E Tamburrini ◽  
V Laghi ◽  
R Cauda ◽  
M Levrero ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Viral Hepatitis ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Acute Viral Hepatitis ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Cryopreservation does not alter the frequency of regulatory T cells in peripheral blood mononuclear cells

2010 ◽  
Vol 353 (1-2) ◽  
pp. 138-140 ◽  
Author(s):  
D. Van Hemelen ◽  
J.N.G. Oude Elberink ◽  
J. Heimweg ◽  
A.J.M. van Oosterhout ◽  
M.C. Nawijn
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals Regulatory T Cells and Their Prognostic Relevance in Hematologic Malignancies

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Giovanni D’Arena ◽  
Candida Vitale ◽  
Marta Coscia ◽  
Agostino Festa ◽  
Nicola Matteo Dario Di Minno ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Peripheral Blood ◽  
Hematological Malignancies ◽  
Mononuclear Cells ◽  
Critical Role ◽  
Prognostic Significance ◽  
Hematologic Malignancies ◽  
Published Data ◽  
Peripheral Blood Mononuclear

Regulatory T cells (Tregs) have a fundamental function in monitoring the immune homeostasis in healthy individuals. In cancer and, in particular, in hematological malignancies, Tregs exert a major immunosuppressive activity, thus playing a critical role in tumor cell growth, proliferation, and survival. Here, we summarize published data on the prognostic significance of Tregs in hematological malignancies and show that they are highly conflicting. The heterogeneity of the experimental approaches that were used explains—at least in part—the discordant results reported by different groups that have investigated the role of Tregs in cancer. In fact, different tissues have been studied (i.e., peripheral blood, bone marrow, and lymph node), applying different methods (i.e., flow cytometry versus immunohistochemistry, whole blood versus isolated peripheral blood mononuclear cells versus depletion of CD25+ cells, various panels of monoclonal antibodies, techniques of fixation and permeabilization, and gating strategies). This is of relevance in order to stress the need to apply standardized approaches in the study of Tregs in hematological malignancies and in cancer in general.

scholarly journals MicroRNA-146a induces immune suppression and drug-resistant colorectal cancer cells

Tumor Biology ◽  
2017 ◽  
Vol 39 (5) ◽  
pp. 101042831769836 ◽  
Author(s):  
Samaneh Khorrami ◽  
Ahmad Zavaran Hosseini ◽  
Seyed Javad Mowla ◽  
Masoud Soleimani ◽  
Naser Rakhshani ◽  
...  
Keyword(s):  
Drug Resistance ◽  
T Cells ◽  
Regulatory T Cells ◽  
Cancer Cells ◽  
Immune Suppression ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Ht 29

Recent studies underline the involvement of microRNAs in cancer development through induction of immune suppression milieu and evolution of drug resistance. The goal of this study was to evaluate the effects of miR-146a on regulatory T cells’ frequencies, T-lymphocyte proliferation, and cytokine expression as well as drug resistance in cancer cells. We found that miR-146a was overexpressed in colon cancer HT-29 cells. Peripheral blood mononuclear cells were obtained from healthy donors and were co-cultured with transfected HT-29 cells. Afterward, peripheral blood mononuclear cell proliferation, expression of anti-inflammatory cytokines, and regulatory T cells’ frequencies were assayed. Also, drug resistance in transfected HT-29 cells was analyzed following treatment with 5-fluorouracil and irinotecan. Overexpression of miR-146a increased transforming growth factor-β and interleukin-10 expressions and enhanced regulatory T cells’ frequencies in peripheral blood mononuclear cells. Also, the number of cells undergoing cell cycle arrest and apoptosis significantly decreased in transfected HT-29 cancer cells. In conclusion, upregulation of miR-146a plays an important role in enhancing immune suppression through increasing the regulatory T cells’ population. Also, our data indicated that colon cancer drug resistance is possibly associated with miR-146a overexpression.

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