Evaluation of serological test in the diagnosis of Helicobacter pylori and risk factors associated with the infection

Background: Serological testing has been widely used for the diagnosis of H. pylori. This study aimed to evaluate the serological test and to determine the sensitivity and specificity of the test in the diagnosis of H. pylori. The study also aimed to address if there are risk factors like blood grouping, Smoking, Age, gender, and residence of the patients associated with H. pylori infection.Methods: A prospective cross‑sectional study was performed among 100 symptomatic patients attending Dr. Suliman dispensary, Elnehoud city in west Kordofan state-Sudan, from March to September 2016. H. pylori were detected on plasma by using Healgen immunochromatography test cards from Xiamen Boson Biotech Co., Ltd (China), and identified from a stool by using monoclonal antigen detection from the same trademarked company. Data for the risk factors associated with the infection were assessed in a participant interview.Results: The serological test showed significant differences when compared to the stool antigen test p-value = 0.000. The statistical analysis showed moderate sensitivity and high specificity of the serological test compared to the stool antigen detection test. The study also showed that smoking [odds ratio (OR): 1.20, 95% confidence interval (CI): (1.24-4.02) and blood grouping (OR: 1.10, 95% CI: (1.08-1.60) were risk factors for H. pylori infection.Conclusions: The serological test showed high specificity and moderate sensitivity in comparison to the stool antigen test. The increased risk of H. pylori infection associated with smoking and blood grouping.

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Evaluation of a stool antigen test using a mAb for native catalase for diagnosis of Helicobacter pylori infection in children and adults

Journal of Medical Microbiology ◽

10.1099/jmm.0.077370-0 ◽

2014 ◽

Vol 63 (12) ◽

pp. 1621-1625 ◽

Cited By ~ 10

Author(s):

Masumi Okuda ◽

Takako Osaki ◽

Shogo Kikuchi ◽

Junko Ueda ◽

Yingsong Lin ◽

...

Keyword(s):

Helicobacter Pylori ◽

Real Time ◽

Real Time Pcr ◽

High Specificity ◽

Antigen Test ◽

Stool Antigen Test ◽

Non Invasive ◽

Significant Difference ◽

Stool Antigen ◽

H Pylori

Non-invasive diagnosis of Helicobacter pylori infection is important not only for screening of infection but also for epidemiological studies. Stool antigen tests are non-invasive and are convenient to identify H. pylori infection, particularly in children. We evaluated the stool antigen test, which uses a mAb for native catalase of H. pylori developed in Japan. A total of 151 stool samples were collected from participants (52 children and 99 adults) of the Sasayama Cohort Study and stored between −30 and −80 °C. The stool antigen test used was Testmate pylori antigen (TPAg), and was performed according to the manufacturer’s instructions. Furthermore, we conducted a quantitative real-time PCR test and compared the PCR results with those of the TPAg test. When compared with the results in real-time PCR, the sensitivity of TPAg was 89.5 % overall, 82.7 % for children and 92.4 % for adults, and the specificity was 100 %. The accuracy was 93.4 % overall, 90.4 % for children and 94.9 % for adults, and there was no significant difference in the accuracy of TPAg between children and adults. Five of 28 children (18 %) and five of 38 adults (13 %) were PCR positive with negative TPAg results. Four of five children with positive PCR and negative TPAg results were given a 13C-urea breath test and all four children tested negative. No significant correlation was observed between the TPAg results and DNA numbers of H. pylori in faeces among children or adults. A stool antigen test (TPAg) using a mAb for native catalase is useful for diagnosis of H. pylori in children and adults. Additionally, this test has particularly high specificity.

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Prevalence and factors associated with Helicobacter pylori infection among treatment naïve dyspeptic adults in University of Benin Teaching Hospital, Benin City, Nigeria

African Journal of Clinical and Experimental Microbiology ◽

10.4314/ajcem.v21i2.3 ◽

2020 ◽

Vol 21 (2) ◽

pp. 97-105

Author(s):

A.O. Odigie ◽

A.J. Adewole ◽

A.A. Ekunwe

Keyword(s):

Helicobacter Pylori ◽

Teaching Hospital ◽

Antigen Test ◽

Cross Sectional ◽

Stool Antigen Test ◽

Factors Associated ◽

Benin City ◽

Treatment Naïve ◽

Stool Antigen ◽

H Pylori

Background: Infectious diseases such as malaria, HIV/AIDS and tuberculosis have received tremendous attention globally but inspite of the widespread nature of infections caused by Helicobacter pylori, little attention has been paid to it especially in the developing countries. The objective of this study was to determine the prevalence of and factors associated with H. pylori infection among dyspeptic patients attending the University of Benin Teaching Hospital, Benin City, Nigeria. Methodology: This was a hospital based descriptive cross-sectional study of 354 treatment naive dyspeptic patients aged 18 to 44 years, recruited consecutively after obtaining institutional ethical approval and subjects’ informed consent. A pre-tested interviewer administered questionnaire was used to obtain subjects’ data. The stool antigen test was used to detect H. pylori infection. Results: The prevalence of H. pylori infection among the subject participants was 34.2% (38.4% in female, 24.0% in male, p=0.009). The age group < 20 years had the highest prevalence of 40%. Gender, occupation, increased body mass index, high number of household occupants, and rural dwelling, were significantly associated with H. pylori infection (p<0.05) and alcohol intake was inversely related to H. pylori infection (p<0.05). Conclusion: The prevalence of H. pylori infection in University of Benin Teaching Hospital is high and factors such as gender, obesity, occupation with risk of contact, low socio-economic status, and poor hygiene, may be responsible for this.Keywords: Dyspepsia, Helicobacter pylori, infection, stool antigen test

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A stool antigen test (HpSA) for the detection of H. pylori infection

Digestive and Liver Disease ◽

10.1016/s1590-8658(00)80805-1 ◽

2000 ◽

Vol 32 ◽

pp. A158

Author(s):

G. Iaquinto ◽

A. Todisco ◽

N. Giardullo ◽

V. D'Onofrio ◽

L. Pasquale ◽

...

Keyword(s):

Antigen Test ◽

Stool Antigen Test ◽

Stool Antigen ◽

H Pylori

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Evaluation of a novel rapid H. pylori stool antigen test: Is a reliable diagnosis now possible in the doctor's office?

Gastroenterology ◽

10.1016/s0016-5085(03)80286-x ◽

2003 ◽

Vol 124 (4) ◽

pp. A58 ◽

Cited By ~ 3

Author(s):

Andreas Leodolter ◽

Kathlen Wolle ◽

Thomas Wex ◽

Ulrich Peitz ◽

Peter Malfertheiner

Keyword(s):

Antigen Test ◽

Stool Antigen Test ◽

Reliable Diagnosis ◽

Stool Antigen ◽

H Pylori ◽

Doctor's Office

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A novel monoclonal stool antigen test for the detection of H. pylori before and after eradication therapy: A prospective study in outpatients

Gastroenterology ◽

10.1016/s0016-5085(03)80880-6 ◽

2003 ◽

Vol 124 (4) ◽

pp. A177 ◽

Cited By ~ 2

Author(s):

Vincens Weingart ◽

Holger Ruessmann ◽

Sibylle Koletzko ◽

Josef Weingart ◽

Wilhelm Hoechter ◽

...

Keyword(s):

Prospective Study ◽

Eradication Therapy ◽

Antigen Test ◽

Stool Antigen Test ◽

Before And After ◽

Stool Antigen ◽

H Pylori ◽

A Prospective Study

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Investigating the Efficacy of Triple Drug Therapy and Sequential Drug Therapy in the Eradication of Helicobacter Pylori with Respect to Antigen Stool test: A Pilot Study

Kathmandu University Medical Journal ◽

10.3126/kumj.v18i1.33367 ◽

2020 ◽

Vol 18 (1) ◽

pp. 74-83

Author(s):

R. Shrestha ◽

R. Poudel ◽

S. Shakya ◽

R.B. Gurung ◽

R. Makaju ◽

...

Keyword(s):

Helicobacter Pylori ◽

Drug Therapy ◽

Sequential Therapy ◽

Drug Regimen ◽

Epidemiological Studies ◽

Response To Treatment ◽

Antigen Test ◽

Stool Antigen Test ◽

Stool Antigen ◽

H Pylori

Background Helicobacter pylori is one of the most prevalent infectious disease worldwide. The treatment regimens involve mainly two therapies: Standard Triple drug therapy and Sequential drug therapy. Several studies have shown that the sequential therapy has higher eradication rates of H. pylori than the standard triple drug therapy and since proper study on sequential drug therapy and standard triple drug therapy is still lacking in Nepal, this study is attempted to compare efficacy of Sequential Drug Therapy in the eradication of H. pylori in gastritis with respect to the Standard triple drug therapy. Objective To investigate the efficacy of Triple Drug Therapy and Sequential Drug Therapy in the eradication of Helicobacter pylori with respect to Antigen Stool test. Method This study was the prospective study conducted in 62 patients attending the Department of Gastroenterology, Dhulikhel Hospital, meeting the inclusion criteria who were confirmed as H. pylori positive by histopathology and stool antigen test. Patients were randomized into two groups. One group prescribed with Standard triple drug regimen and another group with Sequential drug regimen. Eradication of H. pylori infection was confirmed by repeating the stool antigen test at least five weeks after the completion of the regimen. Result Among the 62 participants included in this study, 54.5% of them were males. Among the study population, the eradication achieved by standard triple drug therapy was 87.8% and 89.6% with Sequential drug therapy. Higher numbers (82.3%) of patients were compliant to the prescribed medication. Forgetfulness was the main reason for missing the dose (91%) of the non-compliant patients. Conclusion The study revealed an equal efficacy of both Standard Triple drug regimen and Sequential drug regimen in the eradication of H. pylori infection. Further, Stool antigen test can be preferred as a non-invasive test, for diagnosis of H. pylori infection, monitoring the response to treatment and in epidemiological studies.

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The Use of 13C-Urea Breath Test for Non-Invasive Diagnosis of Helicobacter pylori Infection in Comparison to Endoscopy and Stool Antigen Test

Diagnostics ◽

10.3390/diagnostics10070448 ◽

2020 ◽

Vol 10 (7) ◽

pp. 448

Author(s):

Hamed Alzoubi ◽

Asma’a Al-Mnayyis ◽

Ibrahim Al rfoa ◽

Amin Aqel ◽

Mohammad Abu-Lubad ◽

...

Keyword(s):

Breath Test ◽

Eradication Therapy ◽

Urea Breath Test ◽

P Value ◽

Antigen Test ◽

Stool Antigen Test ◽

Non Invasive ◽

Stool Antigen ◽

13C Urea Breath Test ◽

H Pylori

Helicobacter pylori (H. pylori) can cause gastritis, peptic ulcer diseases and gastric carcinoma. Endoscopy as the gold standard method of diagnosis is an invasive procedure that might not be suitable in all scenarios. Therefore, this first study in Jordan aimed to assess the non-invasive 13C urea breath test (UBT) and stool antigen test for diagnosis of H. pylori infection and the successfulness of eradication therapy as alternatives for endoscopy. Hence, a total of 30 patients attending the endoscopy units at Alkarak teaching hospital were asked to complete a questionnaire with demographic and clinical data. They were then tested for H. pylori using 13C UBT and H. pylori stool antigen before having endoscopy. Another 30 patients who were positive for H. pylori by endoscopy were tested using both tests 6 weeks post eradication therapy. Results showed that the rate of H. pylori detection using endoscopy was 56.7% (17/30). Heartburns (82.3%, p value = 0.019), epigastric pain (88.2%, p value = 0.007) and vomiting (70.5%, p value = 0.02) were the most significant symptoms. Family history of peptic ulcer diseases was significantly associated with an increased risk for having a H. pylori positive result (p value = 0.02). Compared to endoscopy, the sensitivity of 13C UBT for the diagnosis of H. pylori was 94.1% (16/17), while it was 76.5% (13/17) for the stool antigen test. The specificity of both tests was equal (76.9%). However, the positive predictive and negative predictive values (84.2% and 90.9%) for 13C UBT were higher than those (81.3% and 71.4%) for the stool antigen test. The accuracy of 13C UBT was 86.7% compared to 76.7% for the stool antigen test. There was an 87% agreement (20 patients out of 23) between both tests when used to assess success of the eradication therapy. In conclusion, the 13C UBT was found to be more sensitive and accurate than the stool antigen test when used for diagnosis; furthermore, it has a comparable outcome to the stool antigen test in assessing the successfulness of the eradication treatment.

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Evaluation of Rapid H. pylori Stool Antigen Test before and after Eradication Therapy

The American Journal of Gastroenterology ◽

10.14309/00000434-200509001-00140 ◽

2005 ◽

Vol 100 ◽

pp. S69-S70 ◽

Cited By ~ 1

Author(s):

Akifumi Tanaka ◽

Kengo Tokunaga ◽

Kyoto Imase ◽

Hajime Sugano ◽

Hitoshi Ishida ◽

...

Keyword(s):

Eradication Therapy ◽

Antigen Test ◽

Stool Antigen Test ◽

Before And After ◽

Stool Antigen ◽

H Pylori

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Validation of a rapid stool antigen test for diagnosis of Helicobacter pylori infection

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652010000300002 ◽

2010 ◽

Vol 52 (3) ◽

pp. 125-128 ◽

Cited By ~ 12

Author(s):

Joyce Matie Kinoshita da Silva ◽

Cibele Aparecida Villares ◽

Maria do Socorro Monteiro ◽

Carlos Colaúto ◽

Anibal Ferreira dos Santos ◽

...

Keyword(s):

Helicobacter Pylori ◽

Breath Test ◽

False Negative ◽

Lateral Flow ◽

Antigen Test ◽

Kappa Index ◽

Stool Antigen Test ◽

Infection Diagnosis ◽

Stool Antigen ◽

H Pylori

The aim of this study was to validate the rapid lateral flow Helicobacter pylori stool antigen test (One step H. pylori antigen test, ACON laboratories, San Diego, USA; Prime diagnostics, São Paulo), using 13C-Urea Breath Test as the gold standard for H. pylori infection diagnosis. A total of 98 consecutive patients, asymptomatic or dyspeptic, entered the study. Sixty-nine were women, with a mean age of 45.76 ± 14.59 years (14 to 79 years). In the H. pylori-positive group, the rapid stool antigen test detected H. pylori antigen in 44 of the 50 positive patients (sensitivity 88%; 95% CI: 75.7-95.5%), and six false-negative; and in the H. pylori-negative group 42 presented negative results (specificity 87.5%; 95% CI: 74.7-95.3%), and six false-positive, showing a substantial agreement (Kappa Index = 0.75; p < 0.0001; 95% CI: 0.6-0.9). Forty four of fifty patients that had positive stool antigen were H. pylori-positive, the PPV of the stool antigen test was 88% (95% CI: 75.7-95.5%), and 42 patients with negative stool antigen test were H. pylori-negative, the NPV of the stool antigen test was 87.5% (95% CI: 74.7-95.3%). We conclude that the lateral flow stool antigen test can be used as an alternative to breath test for H. pylori infection diagnosis especially in developing countries.

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