scholarly journals Association of M55L and Q192R polymorphisms of paraoxonase 1 gene (PON1) with recurrent pregnancy loss risk: A case–control study

2021 ◽  
Author(s):  
Mehdi Alizadeh ◽  
Mahboobeh Nasiri ◽  
Morteza Samadi ◽  
Nasrin Ghasemi ◽  
Ali Moradi
Keyword(s):  
Statistical Analysis ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Case Control Study ◽  
Case Control ◽  
Paraoxonase 1 ◽  
Control Group ◽  
Case Group ◽  
Significant Difference ◽  
Control Study

Background: Recurrent pregnancy loss (RPL) refers to the incidence of two or more abortions before the first half of pregnancy. Oxidative stress has been hypothesized to play a central role in RPL. Objective: To investigate the relationship between Q192R and L55M polymorphisms of PON1 as antioxidant enzyme and the risk of RPL. Materials and Methods: In this case–control study, 110 women with RPL (case) and 110 healthy fertile women (control) referred to the Research and Clinical Center for Infertility, Shiraz, Iran were enrolled. Genomic DNA was extracted from the peripheral blood in all participants. Polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism method. Results: Statistical analysis of Q192R polymorphism showed a significant difference for the RR genotype between the case and control group (OR = 11, CI = 1.39–86.87, p = 0.005) but none for the QR and QQ genotypes. No significant association was observed between the R and Q allelic frequency in the RPL participants compared to the control group (p = 0.53). Also, statistical analysis of the L55M polymorphism for MM genotype in the case group compared with the control group showed a significant difference (OR = 3.59, CI = 0.97–13.30, p = 0.042), but none for the LM and LL genotypes. Conclusion: The findings showed a significant correlation between the Q192R polymorphisms and the L55M PON1 enzyme and RPL in this study population. Key words: Pregnancy, Abortion, PON1, Polymorphism, Recurrent pregnancy loss.

scholarly journals Frequency of the rs 14035 polymorphism of RAN gen in recurrent pregnancy loss: A case-control study

2020 ◽  
Author(s):  
Zahrasadat Mortazavifar ◽  
Hamidreza Ashrafzadeh ◽  
Seyed Morteza Seifati ◽  
Nasrin Ghasemi
Keyword(s):  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Case Control Study ◽  
Case Control ◽  
Control Group ◽  
Case Group ◽  
Nuclear Pore ◽  
Normal Delivery ◽  
Significant Difference ◽  
Control Study

Background: Genetic factors could account for recurrent pregnancy loss (RPL). The RAN gene is a member of the ”large RAS family” and a small GTPase that is essential for the translocation of Ribonucleic acid (RNA) and proteins through the nuclear pore. Mutation in the RAN constitutive gene could stop DNA synthesis and alter the expression of genes in the uterus, likely playing a role in recurrent miscarriage. Objective: The aim was to investigate the frequency of RAN (rs 14035) polymorphism in women with RPL compared with women without abortion history. Materials and Methods: In this case-control study, 100 women with at least two consecutive miscarriages before the 20th wk of gestation and having spouses with karyotype and normal sperm parameters as the case group and 100 women with no history of abortion and having at least one successful pregnancy and normal delivery as the control group. The groups were age matched (20-40 yr). The rs 14035 polymorphism of RAN gene was investigated by Polymerase Chain Reaction- Restriction Fragment Length poly morphism technique and the frequency of which was compared between the two groups. Results: The frequency of TT, TC, and CC genotypes of RAN gene polymorphism in the case group were 9%, 40%, and 51%, respectively, and in the control group were 11%, 38%, and 51%, respectively. There was no significant difference in the genotypes between two groups (p = 0.882). Conclusion: According to our results, it seems that RAN polymorphism (rs 14035) is not associated with the risk of RPL in this study population. Key words: RAN gene, Repeated abortion, Polymorphism, PCR-RFLP.

scholarly journals High Fasting Insulin Levels and Insulin Resistance May Be Linked to Idiopathic Recurrent Pregnancy Loss: A Case-Control Study

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Corina-Alina Ispasoiu ◽  
Radu Chicea ◽  
Florin Vasile Stamatian ◽  
Florin Ispasoiu
Keyword(s):  
Insulin Resistance ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Case Control Study ◽  
Fasting Glucose ◽  
Case Control ◽  
Control Group ◽  
Case Group ◽  
Fasting Insulin ◽  
Control Study

Objective. Patients with more than two spontaneous pregnancy losses are diagnosed with recurrent pregnancy loss. The aim of this study was to evaluate the IR (insulin resistance) in patients with idiopathic recurrent pregnancy loss.Material and Method. A single center, case control study was performed on one hundred eighteen women divided into case group (with at least two pregnancy losses, earlier than 20 weeks of gestation, and negative for the recurrent pregnancy loss testing) and control group (with at least one live birth, no pregnancy loss). FG (fasting glucose) and FI (fasting insulin) were determined for all patients. IR was evaluated by HOMA-IR index.Results. There were not significant differences between the mean age and BMI in cases and controls (P>0.05). Fasting glucose was significantly higher in the control group (85.6 versus 79.8P<0.01), but fasting insulin (15.24 versus 12.83,P<0.001) and HOMA-IR (2.98 versus 2.69,P<0.05) were significantly higher in the case group.Conclusion. In women with idiopathic recurrent pregnancy loss FI and IR are higher than those in women without spontaneous abortion.

Effects of interleukin-1 receptor antagonist (IL-1Ra) gene 86 bp VNTR polymorphism on recurrent pregnancy loss: a case-control study

2017 ◽  
Vol 30 (3) ◽  
Author(s):  
Yasamin Sayed Hajizadeh ◽  
Elina Emami ◽  
Marina Nottagh ◽  
Zahra Amini ◽  
Nazila Fathi Maroufi ◽  
...  
Keyword(s):  
Receptor Antagonist ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Case Control Study ◽  
Interleukin 1 ◽  
Case Control ◽  
Control Group ◽  
Case Group ◽  
Vntr Polymorphism ◽  
Control Study

AbstractObjectiveRecurrent pregnancy loss (RPL) is a heterogeneous disease which is defined as two or more consecutive fetal losses during early pregnancy. Interleukin-1 receptor antagonist (Materials and methodsIn this case control study, genetic polymorphism was studied in 140 RPL patients and 140 healthy women as controls. Genomic DNA was extracted from the blood samples and polymorphism analysis was performed using the polymerase chain reaction (PCR) method. Finally, the data obtained were analyzed by statistical software.ResultsWe found an increased frequency of the IL-1Ra 1/1 genotype in the case group compared to the control group. Whereas, the frequency of IL-1Ra genotype 1/2 was higher in control group than in the case group. However, we did not observe an association betweenConclusionVNTR polymorphism may not be a genetic factor for RPL. However, investigation of

scholarly journals The Relationship between Serum Levels of Vitamin C, Uric Acid and Antioxidant Condition with Coronary Artery Diseases: a Case-Control Study

2019 ◽  
Author(s):  
Aida Torkzaban ◽  
Seyed Amir Mansour Alavi Naeini ◽  
Akbar Hassanzadeh ◽  
Mehrdad Namdari
Keyword(s):  
Uric Acid ◽  
Vitamin C ◽  
Case Control Study ◽  
Demographic Data ◽  
Systolic Pressure ◽  
Case Control ◽  
Control Group ◽  
Case Group ◽  
Significant Difference ◽  
Control Study

Abstract Background Coronary hearth diseases are among the main causes of death in adults. Increase of oxidative stress and defects in antioxidant defense play a major role in endothelium performance and are an effective factor in progress of atherosclerosis. Some studies have also reported different malondialdehyde and total antioxidant capacity among the atherosclerosis patients.Methods In this case-control study, 44 atherosclerosis patients referring to Shahid Madani treatment-education center were considered as the case group; while 44 healthy peoples were placed in the control group. Demographic data and anthropometric indices were measured. Food frequency questionnaire and international physical activity questionnaires were also completed. After 12 hours of fasting, 10 ml blood was sampled from the participants. Uric acid, vitamin C, TAC and MDA were also measured. The data were finally analyzed by SPSS Ver 22 software.Results A significant difference was observed between the two groups in terms of uric acid (P<0.001) and vitamin C (P<0.03). However, mean MDA and TAC showed no significant difference between the two groups. The two groups’ difference in terms of vitamin A, E and beta carotene, zinc and selenium intake was not significant. A significant difference was however detected between the two groups in terms of vitamin C (P<0.047). A significant relationship was also observed between the systolic pressure and CHD (P<0.028).Conclusion Results of this study indicated that the uric acid and vitamin C levels of atherosclerosis patients had significant increase and decrease in comparison with the healthy subjects, respectively. Mean TAC and antioxidant levels of their diets (except for vitamin C) showed no significant difference. Systolic blood pressure of the patients was significantly higher than the controls.

scholarly journals Assesment of Endometrial Vascularization with Immunohistochemical CD34 Assay in Recurrent Pregnancy Loss: A Case Control Study

2017 ◽  
pp. 1
Author(s):  
Emine Aydın ◽  
Taner Usta
Keyword(s):  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Case Control Study ◽  
Transmembrane Protein ◽  
Vessel Diameter ◽  
Control Group ◽  
Control Groups ◽  
Significant Difference ◽  
And Control ◽  
Control Study

<p><strong>Objectives:</strong> We compared the endometrial vascularization in hysteroscopic endometrial samplings between recurrent pregnancy loss (RPL) and control group.</p><p><strong>Study Design:</strong> We prospectively evaluated hysteroscopic endometrial samplings from RPL and control groups. CD34 transmembrane protein was used for evaluating endometrial vascularization. The vascularization was assessed based on thickness of vessels, diameter of the largest vessel, and number of vessels per mm2 in CD34-stained slides.</p><p><strong>Results:</strong> There was no significant difference in demographic findings and vascularization, such as largest vessel diameter (p: 0.572), and number of vessels per mm2 (p: 0.982) between the two groups.</p><p><strong>Conclusion:</strong> The cycling endometrium is a highly angiogenic tissue and may play a role in the etiology of RPL. However, we find a weak relationship between endometrial vascularization and RPL.</p>

scholarly journals Tooth eruption sequence and dental crowding: a case-control study

F1000Research ◽  
2014 ◽  
Vol 3 ◽  
pp. 122 ◽  
Author(s):  
Vahid Moshkelgosha ◽  
Negar Khosravifard ◽  
Ali Golkari
Keyword(s):  
Case Control Study ◽  
Case Control ◽  
Mixed Dentition ◽  
Control Group ◽  
Case Group ◽  
Significant Difference ◽  
The Mean ◽  
Mandibular Canine ◽  
Dental Crowding ◽  
Control Study

When cases of dental crowding are identified and diagnosed promptly, interceptive orthodontics is particularly successful.Aim: To assess the differences in the eruption sequence of the mandibular canine and first premolar teeth in children with and without dental crowding.Materials and Methods: Children who attended the Shiraz Dental School's orthodontic clinic (Iran) from September to December 2012 were enrolled in this case-control study. Tooth size arch length discrepancy (TSALD) of all 8-10 year olds was calculated from patients’ dental models. Thirty-six children were randomly selected from those with TSALD of equal or less than 4mm (those with crowding). Each selected case was matched for sex and age with another child (as control) with TSALD>−4mm attending the same clinic, in the same time period. The existing panoramic radiographs were traced and the eruption percentages were measured for mandibular canine and first premolar teeth. The mean difference between canine and first premolar eruption percentages was compared between the case and control groups using the SPSS (version PASW 18) software and a paired sample t-test.Results: Canine and first premolar eruption percentages in the case group were 65.82±13.00 and 78.92±10.15 percent, respectively. The mean eruption percentages for canines and first premolars of the control group were 74.12±14.55 and 75.47±11.60 percent, respectively. There was a significant difference in pre-eruptive positions of canine and first premolar teeth in those with moderate to severe crowding when compared to the control group (p<0.001).Conclusion: These findings may improve the early diagnosis of children with high risk of developing moderate to severe crowding during mixed dentition.

scholarly journals The Role of Possible Factors Affecting the Risk of Getting Infected by COVID-19 in Emergency Medical Technicians: A Case-Control Study

2020 ◽  
Author(s):  
Mostafa Sadeghi ◽  
Peyman Saberian ◽  
Parisa Hasani-Sharamin ◽  
Fatemeh Dadashi ◽  
Sepideh Babaniamansour ◽  
...  
Keyword(s):  
Case Control Study ◽  
Demographic Data ◽  
Case Control ◽  
Emergency Medical Technicians ◽  
Control Group ◽  
Case Group ◽  
Factors Affecting ◽  
Emergency Medical ◽  
Significant Difference ◽  
Control Study

Abstract Background: Emergency medical technicians (EMTs) are at the highest risk of coronavirus disease-19 (COVID-19). Adequate access and knowledge about donning and doffing of personal protective equipment (PPE) can decrease the risk of transmission. The aim of this study was to assess the possible factors associated with increasing risk of COVID-19 among EMTs.Methods: This study was a case-control study conducted in Tehran, Iran during 18th February to 20th April 2020. Case group was consisted of confirmed COVID-19 EMTs based on the results of reverse transcriptase polymerase chain reaction and/or lung computed tomography scan. Healthy EMTs were randomly selected as control group. Patients were asked to fill out a checklist including demographic data, data related to the work situation (such as number of missions and type of mask and cloth) and PPE precautions.Results: Sixty-eight patients and 148 healthy persons took part in this study as case and control group, respectively. Having two EMTs involved directly in taking care of patients (p < 0.001) and working with a confirmed case teammate (p < 0.001), considering the precautions such as seal check after wearing the mask (p=0.015), covering the hair with a medical hat (p<0.001), not using personal items despite protective clothing (p<0.001), and avoiding contact with the outer surface of clothing while removing (p<0.001) had significant difference in two groups.Conclusion: We found that the type of used face masks (the surgical mask) and the type of used cloths (using NW (disposable, one-piece, non-waterproof protective cloth)) are correlated with the higher risk of COVID-19 in EMTs.

scholarly journals Association of IL-33 gene rs16924159 polymorphism and recurrent pregnancy loss in Iranian Azeri women

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Amir Kamrani ◽  
Seyed Ali Rahmani ◽  
Parisa Mosapour ◽  
Reza Chavoshi
Keyword(s):  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Case Control Study ◽  
Case Control ◽  
Inflammatory Factors ◽  
Case Group ◽  
Healthy Controls ◽  
Salting Out ◽  
Interleukin 33 ◽  
Control Study

AbstractBackgroundRecurrent pregnancy loss (RPL) referred to two or more consecutive abortions before 20th week of pregnancy. The imbalance of inflammatory factors such as interleukins (IL) can be a significant factor in the RPL. The aim of this study was to investigate association of interleukin-33 (IL-33) gene rs16924159 polymorphism and RPL in Iranian Azeri women.Materials and methodsThis case-control study consisted of 100 women with RPL as case group and 100 healthy controls with successful delivery. Genomic DNA was extracted from whole blood samples using salting out method. The fragments of the rs16924159 polymorphism were amplified by PCR and the genotyping was performed using DNA sequencing.ResultsThe obtained results showed that frequency of GA genotype and G allele of rs16924159 polymorphism in the case group was significantly more than healthy controls (p = 0.033).ConclusionsGenerally, we showed that the IL-33 gene rs16924159 polymorphism may play an important role in risk of RPL in the Iranian Azeri women. However, further studies on different races and geographic areas can be useful in identification of effects of rs16924159 polymorphism on RPL.

scholarly journals Case-control Study on p73 rs1801173 C > T Gene Polymorphism and Susceptibility to Gastric Cancer in a Chinese Han Population

2020 ◽  
Author(s):  
Xuyu Gu ◽  
Xiaoyan Wang ◽  
Huiwen Pan ◽  
Zhenjun Gao ◽  
Guowen Ding ◽  
...  
Keyword(s):  
Frequency Distribution ◽  
Case Control Study ◽  
Case Control ◽  
Chinese Han Population ◽  
Control Group ◽  
Case Group ◽  
Chinese Han ◽  
Han Population ◽  
Significant Difference ◽  
Control Study

Abstract Objective: This study aimed to investigate the association between p73 C14T (rs1801173) polymorphism and the risk of GC in a Chinese Han population. Methods: A hospital-based case-control study was conducted. A total of 577 GC cases and 678 normal controls were recruited. Their genotypes were determined using the SnapShot method. Results: The genotype frequency distribution of the case group and the control group were consistent with the Hardy–Weinberg equilibrium. No significant difference was found in the distribution of gender, age, and drinking history between the case group and the control group. A correlation was observed between smoking and the incidence of GC (P = 0.006). Three genotypes of CC, CT, and TT were found in the rs1801173 locus of p73. The distribution of the dominant model/recessive model did not significantly differ (P = 0.688; 0.937). No statistical difference was found even after adjustment was performed via logistic regression analysis (P = 0.703; 0.990). The frequency distribution between the two groups also did not significantly differ (P = 0.763). Conclusion: Smoking is related to the occurrence and development of GC. No association was found between p73 rs1801173 C > T SNP and the risk of GC in a Chinese Han population. However, additional larger studies and tissue-specific biological characterization are required to confirm these findings.

scholarly journals The Effect of Diet On Gallstones Formation in Turkish Patients: A Case-Control Study

2020 ◽  
Vol 9 (3) ◽  
pp. 147-153
Author(s):  
Cemile Idiz ◽  
Coskun Cakir
Keyword(s):  
Food Consumption ◽  
Case Control Study ◽  
Stone Formation ◽  
Saturated Fatty Acids ◽  
Case Control ◽  
Control Group ◽  
Case Group ◽  
Gallbladder Stones ◽  
Significant Difference ◽  
Control Study

Aim: There are many defined risk factors for the formation of gallstones and nutrition is one of them. We have investigated if there is a relationship between diet and gallstone formation in Turkish gallstone patients. Methods: This prospective case-control study were performed between July 2018- March 2019. A food consumption survey was performed to the gallstone patients and control group. The results of demographic and food consumption data of gallstone patients were compared. Results: There were 110 patients with gallbladder stones and 115 controls with no gallbladder stones. There was no significant difference between the two groups in terms of demographic data; but body mass index was higher and regular physical activity was lower in case group. Although case group eat more eggs than the controls, there were no statistically significant difference. In case group, the consumption of snack and cheese, energy intake, more daily fat consumption, more saturated fatty acids, and cholesterol uptake was higher, whereas red meat consumption was lower. Conclusion: A significant association between the gallstone and the nutrition especially in particular nutritional elements supports the fact that, diet is an important risk factor for gallbladder stone formation. Keywords: gallstones, nutrition, cholesterol, diet

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