scholarly journals Liquid based cytology and immunohistochemistry on cytosmears based diagnosis of letterer-siwe disease in a 40 year male

2021 ◽  
Vol 8 (4) ◽  
pp. 508-511
Author(s):  
Preeti Agarwal ◽  
Fatima Khan ◽  
Vaibhav Gupta ◽  
Devanshi Dubey
Keyword(s):  
Cervical Lymph Node ◽  
Inflammatory Cells ◽  
Needle Aspiration ◽  
Bone Lesions ◽  
Low Grade ◽  
Coffee Bean ◽  
Lower Lung ◽  
Cervical Swelling ◽  
S 100 ◽  
Liquid Based Cytology

: Histiocytes with coffee bean like nucleus are the diagnostic hallmark of Langerhans cell histiocytosis (LCH) supported with immunohistochemical (IHC) demonstration of fascin, CD1a and S-100. We report a case of Letterer-Siwe disease in a 40year male diagnosed on cytology and IHC on cytosmears along with cytomorphology in Liquid based cytology (LBC) smears. Forty year male presented with complains of low grade fever and reduced appetite from two years with a cervical swelling and discomfort from five months. Fine needle aspiration(FNA) from cervical lymph node showed sheets of histiocytes in background of mixed inflammatory cells. LBC smears highlighted fine chromatin, delicate nuclear outline with coffee bean like morphology. On IHC tumor cells were positive for S-100 and CD1a. CT thorax revealed consolidation in left lower lung, bone lesions were found on skeletal survey. Hence diagnosis of Letterer-Siwe disease was given. Patient responded well to systemic chemotherapy.

scholarly journals Mucinous cystic adenoma with inflammatory cell infiltration around the splenic artery mimicking pancreatic cancer: a case report

2020 ◽  
Vol 13 (6) ◽  
pp. 1360-1366
Author(s):  
Hiroaki Okuse ◽  
Reiko Yamada ◽  
Kyosuke Tanaka ◽  
Noriyuki Horiki ◽  
Yoshiyuki Takei
Keyword(s):  
Distal Pancreatectomy ◽  
Splenic Artery ◽  
Inflammatory Cell ◽  
Inflammatory Cells ◽  
Needle Aspiration ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Low Grade ◽  
Cystic Neoplasms ◽  
Cystic Adenoma

AbstractA 45-year-old woman presented with upper abdominal and back pain. A cystic lesion in the pancreas and inflammation around the splenic artery were detected by computed tomography. Although imaging studies were difficult to exclude malignancy, pathological and cytological findings of a fine-needle aspiration showed no signs of malignancy. The patient was, therefore, followed-up for 3 months, during which time the cyst increased in size and developed a cyst-in-cyst structure. She was diagnosed with mucinous cyst neoplasm and underwent distal pancreatectomy. Histologically, the patient was diagnosed as low-grade mucinous cystic adenoma. Soft tissue shadows around the splenic artery were considered to indicate fibrosis and infiltration of inflammatory cells. After distal pancreatectomy, the patient has been uneventful with symptom resolution. This case highlights the potentially atypical presentation of mucinous cystic neoplasms with inflammatory cell infiltration around the splenic artery.

scholarly journals Fine needle aspiration cytology of cervical lymph node involvement by ovarian serous borderline tumor

CytoJournal ◽  
2016 ◽  
Vol 13 ◽  
pp. 16 ◽  
Author(s):  
Longwen Chen ◽  
Kristina A. Butler ◽  
Debra A. Bell
Keyword(s):  
Lymph Node ◽  
Cervical Lymph Node ◽  
Needle Aspiration ◽  
Lymph Node Involvement ◽  
Low Grade ◽  
Borderline Tumor ◽  
Needle Core Biopsy ◽  
Fine Needle ◽  
Serous Borderline Tumor ◽  
Node Involvement

Serous borderline tumor (SBT) involving a cervical lymph node is extremely rare. In addition, fine needle aspiration (FNA) cytology of the involved cervical lymph node shares tremendous morphologic similarity with other low-grade papillary carcinomas. Thus, it can be easily misdiagnosed as metastatic carcinoma. A 42-year-old female had a history of bilateral SBT and postbilateral salpingo-oophorectomy. She presented with left cervical lymphadenopathy 6 months later. FNA cytology showed a low-grade papillary neoplasm with psammoma bodies. Needle core biopsy along with immunostains was diagnostic of cervical lymph node involvement (LNI) of SBT. although extremely rare, cervical LNI can be found in patients with SBTs. FNA cytology, sometimes, is indistinguishable from metastatic papillary adenocarcinoma. Cell block or needle core biopsy is essential to make the correct diagnosis.

scholarly journals Diagnostic algorithm in small pigmented choroid tumors (less than 3 mm thick)

2021 ◽  
Vol 4 ◽  
pp. 5
Author(s):  
David Eduardo Pelayes ◽  
Anibal Martin Folgar ◽  
Pablo Chiaradia ◽  
Jorge Oscar Zarate
Keyword(s):  
Diagnostic Algorithm ◽  
Needle Aspiration ◽  
Low Grade ◽  
Systemic Treatments ◽  
Ocular Ultrasound ◽  
S 100 ◽  
Controversial Topic ◽  
Hematoxylin And Eosin ◽  
Age Range

Objectives: The aim of this paper is to present a diagnostic algorithm for a controversial topic in ophthalmic oncology, small pigmented choroidal tumors (<3 mm thick). Material and Methods: Nineteen consecutive patients with a clinical diagnosis of small choroidal pigmented tumors were included in the study. The group of patients studied consisted of 9 patients (47.36%) female and 10 patients (52.64%) male, the age range was 14–68 years. All cases were ophthalmologically evaluated, including best-corrected visual acuity, anterior and posterior segment biomicroscopy, intraocular pressure, binocular indirect ophthalmoscopy, and as additional complementary examinations, ocular ultrasound was performed, which in 100% of the cases was inconclusive, optical coherence tomography, autofluorescence, and angiography in selected cases according to location and symptomatology. In 13 patients (68.50%), transvitreous puncture was performed with or without vitrectomy. Trans-scleral puncture was performed in 6 patients (31.50%), located at the equator (4 patients) and ciliary body (2 patients). Post-surgical follow-up was performed within the first 3 weeks after the procedure and then controlled every 3 months within the 1st year. The material obtained by fine-needle aspiration (FNA) was placed in non-hemolytic preservative liquid. Hematoxylin and eosin, Pas, Masson’s trichrome, and immunohistochemistry (HMB 45, MELAN A, PROT. S-100 base) were performed. Results: The yield of cytologic material was 100% in the sampled patients. The most frequent complication was subretinal hemorrhage in three patients with transvitreous access and two patients with trans-scleral access, in all cases, there was a favorable evolution without requiring further action. In three patients, there were mild vitreous hemorrhages that resolved spontaneously, all of them had undergone transvitreous access. Conclusion: In TPPC of less, we propose a diagnostic algorithm with FNA to obtain cytological sample which allows not only the diagnosis of certainty to indicate treatment but also to determine cytological and molecular prognostic factors that allow classifying melanoma of high or low grade and potentially in case of metastatic disease to indicate systemic treatments. We believe that it is essential to diagnose this type of lesions in which a diagnosis of certainty is required. The alternative is the observation that we consider potentially dangerous in these cases.

Rapid Cell Transfer by Means of Nylon Mesh to Improve Cellular Diagnosis: The Role of Immunocytochemistry

2021 ◽  
pp. 1-6
Author(s):  
Satoshi Morito ◽  
Takao Nitanda ◽  
Ryuko Tsukamoto ◽  
Shingo Kamoshida ◽  
Hiroshi Yasui ◽  
...  
Keyword(s):  
Needle Aspiration ◽  
Turnaround Time ◽  
Body Cavity ◽  
Cell Transfer ◽  
Special Equipment ◽  
Nylon Mesh ◽  
Cytological Smear ◽  
Liquid Based Cytology ◽  
Transfer Method

Immunocytochemistry (ICC) is an important ancillary technique in clinical cytology for not only identifying and characterizing tumor cells but also gaining prognostic or therapeutic information. Although cell blocks are often prepared for immunocytochemical evaluation of body cavity fluid and fine-needle aspiration specimens, they are not suitable for hypocellular samples. Liquid-based cytology can help prepare additional smears from residual cytological specimens. However, since conventional methods are used for nongynecological specimens in most laboratories, ICC is often limited by the number of cytological smears. Cell transfer methods permit to evaluate several immunocytochemical markers in a single cytological smear. Yet, these methods have some limitations; for example, they are time-consuming (about 3–40 h) and medium membranes with their attached cells are occasionally stretched or torn when peeled off the slides. Therefore, in an attempt to solve these problems, we developed a rapid and reliable cell transfer method using a nylon mesh. Our method requires no special equipment or reagent and can significantly reduce the turnaround time, as compared to previous methods.

Pitfalls in Salivary Gland Fine-Needle Aspiration Cytology: Lessons From the College of American Pathologists Interlaboratory Comparison Program in Nongynecologic Cytology

2005 ◽  
Vol 129 (1) ◽  
pp. 26-31 ◽  
Author(s):  
Jonathan H. Hughes ◽  
Emily E. Volk ◽  
David C. Wilbur
Keyword(s):  
Salivary Gland ◽  
Fine Needle Aspiration ◽  
False Positive ◽  
Interlaboratory Comparison ◽  
False Negative ◽  
Diagnostic Errors ◽  
Poor Performance ◽  
Needle Aspiration ◽  
Low Grade ◽  
Fine Needle

Abstract Context.—We use data from the College of American Pathologists Interlaboratory Comparison Program in Nongynecologic Cytology to identify common diagnostic errors in salivary gland fine-needle aspiration (FNA). Objective.—To identify salivary gland FNA cases with poor performance characteristics in the Nongynecologic Cytology Program surveys, so that the most common diagnostic pitfalls can be avoided. Design.—A retrospective review of the College of American Pathologists Nongynecologic Cytology Program's cumulative data from 1999 to 2003 revealed the most common false-positive and false-negative interpretations on FNA for common salivary gland lesions. Slides that performed poorly were then reviewed to identify the cytologic characteristics that may have contributed to their poor performance. Results.—A total of 6249 participant responses with general interpretations of benign (n = 4642) or malignant (n= 1607) were reviewed. The sensitivity and specificity of the participant responses for correctly interpreting the cases as benign or malignant were 73% and 91%, respectively. Benign cases with the highest false-positive rates were monomorphic adenoma (53% false-positive), intraparotid lymph node (36%), oncocytoma (18%), and granulomatous sialadenitis (10%). Malignant cases with the highest false-negative rates were lymphoma (57%), acinic cell carcinoma (49%), low-grade mucoepidermoid carcinoma (43%), and adenoid cystic carcinoma (33%). Selected review of the most discordant individual cases revealed possible explanations for some of the interpretative errors. Conclusions.—These data confirm the difficulty associated with interpretation of salivary gland FNA specimens. Cytologists should be aware of the potential false-positive and false-negative interpretations that can occur in FNAs from this organ site in order to minimize the possibility of diagnostic errors.

ROLE OF FINE NEEDLE ASPIRATION CYTOLOGY (FNAC) IN BONE TUMORS AND TUMOR LIKE LESIONS

2021 ◽  
pp. 69-70
Author(s):  
Ragini Kumari ◽  
Vikash M. Harinandan ◽  
Kunal Shankar ◽  
Ajit Kumar Chaudhary ◽  
Debarshi Jana
Keyword(s):  
Fine Needle Aspiration ◽  
Bone Tumors ◽  
Needle Aspiration ◽  
Bone Lesions ◽  
Diagnostic Modality ◽  
Predictive Values ◽  
Female Ratio ◽  
Fine Needle ◽  
Needle Aspiration Cytology

Background: FNAC is considered an important procedure in the diagnosis of bone tumors because of its high accuracy. In our study FNA was performed in patients to study the role of FNAC in the diagnosis of bone tumors and to evaluate its usefulness as a diagnostic modality. Methods: Fine needle aspiration was performed on 54 patients whose age ranged between 5 – 75 years with a male to female ratio 1.84 :1 presented with various bone lesions. The bone lesions included 42 (78%) primary lesions, 5 (9.3%) metastatic deposits and 7 (12. 7%) malignancy of related structures involving the bone. Results: 47 biopsy specimens were available for cytohistological correlation and cytological ndings of 45 cases correlated with histopathology. The overall diagnostic accuracy was 95.92%, with 100% sensitivity and specicity. The predictive values of positive as well as negative test were 100%. Conclusions:Thus FNAwas found to be a safe and an extremely useful, rapid method in the preliminary diagnosis of bone lesions.

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