scholarly journals Sulfhydryl variable-5 extended spectrum β-lactamase in nosocomial enteric bacteria causing sepsis in mexican children

2015 ◽  
Vol 36 (5) ◽  
pp. 383
Author(s):  
Angélica Flores-Pérez ◽  
Catalina Gayosso-Vázquez ◽  
María Dolores Alcántar-Curiel ◽  
Patricia Arzate-Barbosa ◽  
Celia Alpuche-Aranda ◽  
...  

<p><strong>Introduction:</strong> Enteric bacteria causing nosocomial infections are often resistant to third-generation cephalosporins due to the production of extended-spectrum β-lactamases (ESBLs).</p><p><br /><strong>Objective:</strong> To describe and characterize the ESBLs pattern present in Klebsiella pneumoniae and Serratia marcescens strains, isolated as causative of nosocomial sepsis in pediatric patients at Instituto Nacional de Pediatría (National Institute of Pediatrics).</p><p><br /><strong>Material and methods:</strong> We analyzed 94 strains of K. pneumoniae and 7 of S. marcescens isolated from clinical specimens from 2002-2005, causative of sepsis in a children’s hospital. We evaluated antibiotic susceptibility and detection of ESBL phenotypes by disk diffusion methods; ceftazidime-resistant isolates were further characterized by pulsed field gel electrophoresis (PFGE); and ESBLs were phenotypically and genotypically characterized by isoelectric focusing, polymerase chain reaction (PCR) and sequencing. We also assed for presence of conjugative plasmids bearing the ESBL gene.</p><p><br /><strong>Results:</strong> 51/94 (54%) of K. pneumoniae isolates, and 5/7 (71%) of S. marcescens isolates were resistant to ceftazidime; all carried a blaSHV-5 gene. All K. pneumoniae isolates had a distinct PFGE profile, yet all carried a ~48-Kb plasmid, that was conjugatively transferable to an Escherichia coli receptor, which expressed the resistance phenotype. On the other hand, all S. marcescens isolates had a similar PFGE profile, were unable to transfer the ceftazidime-resistance phenotype, and were isolated from the same ward in a short time-span suggesting an outbreak.</p><p><strong>Conclusions:</strong> The overall prevalence of ESBL-producing enteric bacteria in this hospital is high but similar to other Latin American reports. The sulfhydryl variable-5 (SHV-5) ESBL gene appears to reside in a highly mobile plasmid, capable of spreading among different K. pneumoniae clones and perhaps even to S. marcescens.</p>

2021 ◽  
pp. 233264922110120
Author(s):  
Christina A. Sue ◽  
Fernando Riosmena

In recent decades, an increasing number of Latin American countries have included ethnoracial questions on their censuses, giving rise to unprecedented data on monoracial and multiracial forms of classification. In Mexico, the government launched a count of its black population for the first time in the nation’s history in 2015, in addition to its long-standing practice of enumerating its indigenous population. Most recently in 2018, it conducted a survey, again asking about both black and indigenous identification. Within this short time span, the black population grew from 1.8 percent to 5.9 percent, becoming a sizable, statistically visible minority. A large majority of black individuals also identified as indigenous, revealing an important form of dual-minority multiracialism. In this article, we analyze these unprecedented data, detailing the size, composition, and growth of these populations. We use the Mexican case to illustrate the potential implications of measuring ethnoracial inequality using single- versus dual-category approaches. We find that black disadvantage is considerably more pronounced when explicitly allowing for multiracial classification. Methodologically, our findings contribute to nascent conversations about how to incorporate the new social and statistical realities of multiracialism in inequality analyses. Theoretically, we expand the multiracialism literature from its traditional focus on part-white mixtures, to a focus on overlapping minority classification. Finally, we build on theories of intersectionality, which generally focus on intersections of oppression across multiple “master statuses” (e.g., race, class, and gender), by also examining intersecting oppressions within the single master status of race.


Religions ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 545
Author(s):  
Gary Carville

The Second Vatican Council and, in particular, its Constitution on the Sacred Liturgy, changed much in the daily life of the Church. In Ireland, a country steeped in the Catholic tradition but largely peripheral to the theological debates that shaped Vatican II, the changes to liturgy and devotional practice were implemented dutifully over a relatively short time span and without significant upset. But did the hierarchical manner of their reception, like that of the Council itself, mean that Irish Catholics did not receive the changes in a way that deepened their spirituality? And was the popular religious memory of the people lost through a neglect of liturgical piety and its place in the interior life, alongside what the Council sought to achieve? In this essay, Dr Gary Carville will examine the background to the liturgical changes at Vatican II, the contribution to their formulation and implementation by leaders of the Church in Ireland, the experiences of Irish Catholic communities in the reception process, and the ongoing need for a liturgical formation that brings theology, memory, and practice into greater dialogue.


Author(s):  
Anders Raustorp ◽  
Andreas Fröberg

Background: The objectives of this study were to explore the effect of time, long-term tracking, and the proportion of objectively measured physical activity (PA) from early adolescence to the mid-thirties. Methods: PA was measured as mean steps per day (SPD) with pedometers during 2000 (T1), 2003 (T2), 2005 (T3), 2010 (T4), 2016 (T5) and 2020 (T6). Data from 64 participants (n = 32 males) were analysed from their early adolescence (T1) to their mid-thirties (T6). Results: SPD decreased in the total sample and among males and females (all, p < 0.001). Males took more mean SPD than females during T1 (p = 0.002), whereas females took more mean SPD during T2 (p = 0.009) and T6 (p = 0.008). Males’ mean SPD tracked between T1 and T2 (p = 0.021), T2 and T3 (p = 0.030), T3 and T4 (p = 0.015) and T4 and T5 (p = 0.003). Females’ mean SPD tracked between T3 and T4 (p = 0.024) and T5 and T6 (p < 0.001). In the total sample, more mean SPD were found on weekdays compared to weekend days at T3 (p = 0.017) and T5 (p < 0.001). Conclusions: SPD decreased between T1 and T6. Mean SPD tracked low-to-moderate in the short time span. From late adolescence to the mid-thirties, more mean SPD was observed during weekdays compared to weekend days.


1999 ◽  
Vol 43 (5) ◽  
pp. 1281-1284 ◽  
Author(s):  
Thierry Naas ◽  
Laurence Philippon ◽  
Laurent Poirel ◽  
Esthel Ronco ◽  
Patrice Nordmann

ABSTRACTA clinical isolate ofPseudomonas aeruginosaRP-1 produced the extended-spectrum β-lactamase (ESBL) SHV-2a. Its gene was expressed from a composite promoter made of the −35 region derived from the left inverted repeat of IS26and the −10 region from theblaSHV-2apromoter itself. The DNA sequences immediately surroundingblaSHV-2awere homologous to plasmid pMPA2a fromKlebsiella pneumoniaeKpZU-3, while further away and 3′ to theblaSHV-2agene, a sequence corresponding to the left end of Tn1721was detected, thus indicating a likely enterobacterial origin of this ESBL gene.


2009 ◽  
Vol 28 (1) ◽  
pp. 53-66 ◽  
Author(s):  
Thijs R. A. Vandenbroucke ◽  
Sarah E. Gabbott ◽  
Florentin Paris ◽  
Richard J. Aldridge ◽  
Johannes N. Theron

Abstract. Isolated chitinozoans from the Soom Shale Member of the Cedarberg Formation, SW South Africa are described and provide a date of the latest Hirnantian–earliest Rhuddanian. The recovered chitinozoans are typical of the latest Ordovician Spinachitina oulebsiri Biozone, although an earliest Silurian age is possible. They indicate a very short time span (less than 1 Ma) across the Ordovician–Silurian boundary. This is currently the highest biostratigraphical resolution attainable for the Soom Shale Lagerstätte. Correlation of the Soom Shale chitinozoans with identical assemblages in post-glacial, transgressive deposits of Northern Africa is possible; both faunas occur in shales that overlie glacial diamictites of the Hirnantian glaciation. A new species, Spinachitina verniersi n. sp. is described.


1995 ◽  
Vol 48 (3) ◽  
pp. 341-358 ◽  
Author(s):  
Elaine L. Graham

One of the most significant phenomena within the Western Church over the past twenty-five years has been the emergence of feminist theology. Fuelled by the second wave of the modern women's movement, drawing upon the theoretical and critical stances of academic feminism, and inspired by Latin American Liberation Theology, feminist theologians have achieved a remarkable body of work in a relatively short time. They have sought to establish the opportunities and validate the methods by which women, long silenced as theological subjects, may articulate their perspectives and contribute towards the reconstruction of a more ‘inclusive’ theological discipline.


2018 ◽  
Vol 15 ◽  
pp. 36-40 ◽  
Author(s):  
Kennedy F. Chah ◽  
Ifeoma C. Ugwu ◽  
Angela Okpala ◽  
Kwabugge Y. Adamu ◽  
Carla Andrea Alonso ◽  
...  

2013 ◽  
Vol 10 (4) ◽  
pp. 95-97
Author(s):  
Mahesh Jayaram ◽  
Ranga Rattehalli ◽  
Lindsay Moran ◽  
John Mwanza ◽  
Paul Banda ◽  
...  

The evidence base for rapid tranquillisation is small in higher-income countries but is even smaller in sub-Saharan Africa. We initiated the first ever survey on the use of rapid tranquillisation in Zambia in 2009; a further survey was then done in 2010, after a programme of teaching and training. It demonstrated an overall improvement in clinical practice, safety, awareness and use of medications within therapeutic doses. It also led to a reduction in inappropriate use of medications. These improvements in practice occurred within a short time span and with minimal effort. Further international collaborative partnerships are required to build stronger mental health infrastructure in Zambia.


Author(s):  
Andrew S. Cohen

Before discussing paleolimnological archives, we need to consider those aspects of limnology that regulate how information is produced, transmitted, and filtered through the water column. Although many limnological processes leave behind sedimentary clues of their existence or intensity and are thus amenable to paleolimnological analysis, others leave little or no detectable trace. Our consideration of limnology here emphasizes the former. Throughout the next three chapters we will examine the properties of lakes, the implications of these properties for paleolimnology, and the types of physical, chemical, and biological information that can be transcribed into sedimentary archives. Physical processes in lakes are of interest because they act as intermediary hydroclimate filters between external forcing events of interest, like climate, and the paleolimnological record. For example, understanding the hydrology of a lake is important because water inputs and outputs, which are often controlled by climate, determine lake levels, which in turn are recorded by ancient shoreline elevations, or indirectly by salinity indicators. Light and heat penetration regulate the distribution of organisms and the mixing of the water column, recorded by the distribution of various fossils, sediment types, and geochemical characteristics of sediments. Also, current and wave activity affect the transport of sedimentary particles and therefore the distribution of sediment types around a lake basin. Understanding these physical processes therefore provides us with a means of linking sedimentological, geochemical, and paleobiological records of lake deposits to the external environment. Water enters and exits lakes through a variety of paths that comprise part of the earth’s hydrological cycle. The lake components of this cycle include a series of inputs and outputs of water, which in combination with the morphometry of the lake basin, collectively determine the lake’s level. Inputs include precipitation, surface runoff from rivers, and groundwater discharge into the lake. Outflows include surface outflow, evaporation, evapotranspiration losses from emergent aquatic plants, groundwater recharge, and hydration reactions with underlying sediments. If water inputs and outputs for a lake are equal over a short time span, the lake surface elevation will remain constant. This is approximately the case in most lakes that are surficially open basins.


2018 ◽  
Vol 39 (1) ◽  
pp. 56-63
Author(s):  
Patricia J. Scott ◽  
Kelsey G. McKinney ◽  
Jeff M. Perron ◽  
Emily G. Ruff ◽  
Jessica L. Smiley

The Role Checklist is used by occupational therapists across the globe. Developed in 1981 and consistent with the Model of Human Occupation (MOHO), until recently, the Role Checklist was not updated. This is of concern as the Role Checklist originally was established to measure role performance. In 2008, Kielhofner, in the fourth edition of A Model of Human Occupation, differentiated occupational performance in 10 roles which clearly fall into occupational participation in the Role Checklist Version 3. The objective of the study was to describe changes and establish utility, feasibility, and reliability of the Role Checklist Version 3. The Role Checklist Version 3 was administered electronically to N = 114 occupational therapists and students. A short time span was used due to sensitivity to history bias. Test–retest reliability using Cohen’s Kappa and Cronbach’s alpha mirrored analysis done on the original version. Qualitatively, nine themes emerged regarding utility and feasibility. Test–retest reliability is acceptable to excellent for present role incumbency (κ = 0.74-1.00), desired future role engagement (κ = 0.44-1.00), and satisfaction with performance (α = 0.77-0.98). Participants (91%) found it useful for treatment planning and 75% would recommend Version 3 over the original Role Checklist. Data support the Role Checklist Version 3 as a reliable, electronic instrument feasible for occupational therapists to measure participation.


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