scholarly journals An SHV-Derived Extended-Spectrum β-Lactamase in Pseudomonas aeruginosa

1999 ◽  
Vol 43 (5) ◽  
pp. 1281-1284 ◽  
Author(s):  
Thierry Naas ◽  
Laurence Philippon ◽  
Laurent Poirel ◽  
Esthel Ronco ◽  
Patrice Nordmann
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Clinical Isolate ◽  
Dna Sequences ◽  
Inverted Repeat ◽  
Esbl Gene ◽  
Extended Spectrum ◽  
Its Gene

ABSTRACTA clinical isolate ofPseudomonas aeruginosaRP-1 produced the extended-spectrum β-lactamase (ESBL) SHV-2a. Its gene was expressed from a composite promoter made of the −35 region derived from the left inverted repeat of IS26and the −10 region from theblaSHV-2apromoter itself. The DNA sequences immediately surroundingblaSHV-2awere homologous to plasmid pMPA2a fromKlebsiella pneumoniaeKpZU-3, while further away and 3′ to theblaSHV-2agene, a sequence corresponding to the left end of Tn1721was detected, thus indicating a likely enterobacterial origin of this ESBL gene.

scholarly journals Interspecies Dissemination of theblaGene Encoding PER-1 Extended-Spectrum β-Lactamase

2010 ◽  
Vol 55 (3) ◽  
pp. 1305-1307 ◽  
Author(s):  
Il Kwon Bae ◽  
Sook Jin Jang ◽  
Juwon Kim ◽  
Seok Hoon Jeong ◽  
Byungkyu Cho ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Acinetobacter Baumannii ◽  
Clinical Isolate ◽  
Horizontal Transfer ◽  
Incompatibility Group ◽  
Gram Negative ◽  
Extended Spectrum ◽  
Group A

ABSTRACTPER-1 extended-spectrum β-lactamase-producing Gram-negative bacilli are resistant to oxyimino-cephalosporins. However, theblaPER-1gene has never been reported inKlebsiella pneumoniae. Here, we studied interspecies dissemination of theblaPER-1gene by horizontal transfer of Tn1213amongAcinetobacter baumannii,Pseudomonas aeruginosa, andK. pneumoniae.In aK. pneumoniaeclinical isolate, theblaPER-1gene was located on a 150-kbp incompatibility group A/C plasmid.

scholarly journals Acquisition of Broad-Spectrum Cephalosporin Resistance Leading to Colistin Resistance in Klebsiella pneumoniae

2016 ◽  
Vol 60 (5) ◽  
pp. 3199-3201 ◽  
Author(s):  
Aurélie Jayol ◽  
Patrice Nordmann ◽  
Marine Desroches ◽  
Jean-Winoc Decousser ◽  
Laurent Poirel
Keyword(s):  
Klebsiella Pneumoniae ◽  
Clinical Isolate ◽  
Broad Spectrum ◽  
Insertion Sequence ◽  
Selective Pressure ◽  
Acquired Resistance ◽  
Colistin Resistance ◽  
Content Type ◽  
Esbl Gene ◽  
Cephalosporin Resistance

ABSTRACTAn extended-spectrum β-lactamase (ESBL)-producing and colistin-resistantKlebsiella pneumoniaeclinical isolate was recovered from a patient who was treated with cefotaxime. This isolate harbored ablaCTX-M-15ESBL gene that was associated with an ISEcp1insertion sequence. Transposition of that tandem occurred within the chromosomalmgrBgene, leading to inactivation of themgrBgene and consequently to acquired resistance to colistin. We showed here a coselection of colistin resistance as a result of a broad-spectrum cephalosporin selective pressure.

scholarly journals Draft Genome Sequence of a Hypermucoviscous Extended-Spectrum-β-Lactamase-Producing Klebsiella quasipneumoniae subsp. similipneumoniae Clinical Isolate

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
U. Garza-Ramos ◽  
J. Silva-Sánchez ◽  
J. Catalán-Nájera ◽  
H. Barrios ◽  
N. Rodríguez-Medina ◽  
...  
Keyword(s):  
Adult Patient ◽  
Clinical Isolate ◽  
Genome Sequencing ◽  
Dna Sequences ◽  
Urine Culture ◽  
Draft Genome ◽  
Trna Genes ◽  
Whole Genome ◽  
Genome Sequences ◽  
Extended Spectrum

A clinical isolate of extended-spectrum-β-lactamase-producing Klebsiella quasipneumoniae subsp. similipneumoniae 06-219 with hypermucoviscosity phenotypes obtained from a urine culture of an adult patient was used for whole-genome sequencing. Here, we report the draft genome sequences of this strain, consisting of 53 contigs with an ~5.6-Mb genome size and an average G+C content of 57.36%. The annotation revealed 6,622 coding DNA sequences and 77 tRNA genes.

scholarly journals Biochemical Characterization of the TEM-107 Extended-Spectrum β-Lactamase in a Klebsiella pneumoniae Isolate from South Korea

2011 ◽  
Vol 55 (12) ◽  
pp. 5930-5932 ◽  
Author(s):  
Kyungwon Lee ◽  
Jong Hwa Yum ◽  
Dongeun Yong ◽  
Seok Hoon Jeong ◽  
Gian Maria Rossolini ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
South Korea ◽  
Clavulanic Acid ◽  
Clinical Isolate ◽  
Biochemical Characterization ◽  
Content Type ◽  
Extended Spectrum

ABSTRACTThe TEM-107 extended-spectrum β-lactamase detected in aKlebsiella pneumoniaeclinical isolate had a Gly238Ser substitution compared to the TEM-43 β-lactamase. The MIC of ceftazidime was higher (64 μg/ml) than that of cefotaxime (2 μg/ml) for the isolate. Clavulanic acid reduced the MIC of ceftazidime 64-fold.

scholarly journals Characterization of TEM-56, a Novel β-Lactamase Produced by a Klebsiella pneumoniae Clinical Isolate

2000 ◽  
Vol 44 (2) ◽  
pp. 453-455 ◽  
Author(s):  
Catherine Neuwirth ◽  
Roger Labia ◽  
Eliane Siebor ◽  
Andre Pechinot ◽  
Stephanie Madec ◽  
...  
Keyword(s):  
Amino Acid ◽  
Klebsiella Pneumoniae ◽  
Amino Acid Sequence ◽  
Clinical Isolate ◽  
Isoelectric Point ◽  
Catalytic Properties ◽  
Resistance Level ◽  
Extended Spectrum ◽  
Moderate Resistance

ABSTRACT TEM-56 produced by a Klebsiella pneumoniae clinical isolate is a novel β-lactamase of isoelectric point 6.4 that confers a moderate resistance level to expanded-spectrum cephalosporins. The amino acid sequence deduced from the corresponding bla gene showed two amino acid replacements with respect to the TEM-2 sequence: Glu-104 to Lys and His-153 to Arg. This enzyme showed catalytic properties close to those of TEM-18. Thus, TEM-56 appears as a new TEM mutant, an intermediary between TEM-18 and the extended-spectrum β-lactamase TEM-21.

scholarly journals SHV-14, a Novel β-Lactamase Variant inKlebsiella pneumoniae Isolates from Nijmegen, The Netherlands

2001 ◽  
Vol 45 (1) ◽  
pp. 309-311 ◽  
Author(s):  
Meifang Yuan ◽  
Lucinda M. C. Hall ◽  
Jaa Hoogkamp-Korstanje ◽  
David M. Livermore
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
The Netherlands ◽  
Klebsiella Pneumoniae ◽  
Extended Spectrum ◽  
Its Gene ◽  
Novel Variant

ABSTRACT Four ceftazidime-resistant isolates of a Klebsiella pneumoniae strain were collected from intensive care unit patients in Nijmegen, The Netherlands. These isolates had TEM-29 and SHV-14 β-lactamases. SHV-14 is a novel variant, with two substitutions compared with the sequence of SHV-1: Ile8Phe and Arg43Ser. Its gene also had a silent C→T mutation at nucleotide 481. The SHV-14 enzyme had slightly higher V maxrates than SHV-1 for oxyimino-aminothiazolyl cephalosporins, but this activity was insufficient for the enzyme to count as an extended-spectrum β-lactamase.

scholarly journals Emergence and rapid global dissemination of CTX-M-15-associated Klebsiella pneumoniae strain ST307

2018 ◽  
Author(s):  
Kelly L. Wyres ◽  
Jane Hawkey ◽  
Marit A.K. Hetland ◽  
Aasmund Fostervold ◽  
Ryan R. Wick ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
Carbapenem Resistance ◽  
Beta Lactamase ◽  
First Report ◽  
Extended Spectrum Beta Lactamase ◽  
Esbl Gene ◽  
Resistance Determinants ◽  
Extended Spectrum ◽  
Globally Distributed

AbstractRecent reports indicate the emergence of a new carbapenemase producing Klebsiella pneumoniae clone, ST307. Here we show that ST307 emerged in the mid-1990s (nearly 20 years prior to its first report), is already globally distributed and is intimately associated with a conserved plasmid harbouring the blaCTX-M-15 extended-spectrum beta-lactamase (ESBL) gene plus other antimicrobial resistance determinants. Our findings support the need for enhanced surveillance of this widespread ESBL clone in which carbapenem resistance is now emerging.

scholarly journals Antagonistic Activities of Cell-Free Supernatants of Lactobacilli Against Extended-Spectrum β-Lactamase Producing Klebsiella pneumoniae and Pseudomonas aeruginosa

2020 ◽  
Vol Volume 13 ◽  
pp. 543-552 ◽  
Author(s):  
Mohamed A El-Mokhtar ◽  
Khaled M Hassanein ◽  
Ahmed S Ahmed ◽  
Gamal FM Gad ◽  
Mohamed M Amin ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Extended Spectrum

scholarly journals Acquisition of a Transposon Encoding Extended-Spectrum β-Lactamase SHV-12 by Pseudomonas aeruginosa Isolates during the Clinical Course of a Burn Patient

2010 ◽  
Vol 54 (9) ◽  
pp. 3956-3959 ◽  
Author(s):  
Shuji Uemura ◽  
Shin-ichi Yokota ◽  
Hirotoshi Mizuno ◽  
Eiji Sakawaki ◽  
Keigo Sawamoto ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Clinical Course ◽  
Resistance Determinant ◽  
Gene Encoding ◽  
Extended Spectrum ◽  
Its Gene ◽  
Burn Patient

ABSTRACT Three of seven clonally related Pseudomonas aeruginosa strains isolated from a burn patient produced the extended-spectrum β-lactamase (ESBL) SHV-12. Its gene was flanked by two IS26 elements with a large transposon (>24 kb). The transposon also contained at least five IS26 elements and a gene encoding the amikacin resistance determinant aminoglycoside 6′-N-acetyltransferase type Ib [aac(6′)-Ib]. It was inserted into the gene PA5317 in the P. aeruginosa chromosome.

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