Effects of mitomycin C at different exposure time on the morphology of corneal endothelial cells in LASEK surgery

2020 ◽  
Vol 1 (2) ◽  
pp. 122-127
Author(s):  
Kai-Yun Zhang ◽  
Keyword(s):  
Endothelial Cells ◽  
Mitomycin C ◽  
Exposure Time ◽  
Corneal Stroma ◽  
Time Range ◽  
Corneal Endothelial Cells ◽  
Average Cell ◽  
Significant Difference ◽  
Acute Changes ◽  
Area Standard

AIM: To investigate the effects of mitomycin C (MMC) on corneal endothelial cells at different exposure time during laser-assisted subepithelial keratomileusis (LASEK). METHODS: Patients who received LASEK were included and divided into Group T1 (15s≤t≤45s) and T2 (5s<t≤70s) based on the exposure time of MMC with corneal stroma. Cell density (CD) of corneal endothelial cells in central and peripheral cornea, average cell area (ACA), area standard deviation (SD), coefficient of variation (CV) and percentage of hexagonal cells (HEX) before surgery and at 1wk, 1, 3 and 6mo after surgery were compared between and within the groups. RESULTS: A total of 196 eyes of 98 patients were included with 98 eyes in Group T1 and 98 eyes in Group T2. With the exception of peripheral ACA, central CV and HEX in Group T1 and T2, which significantly changed at 1wk after the surgery (P<0.05), there was no significant difference in central and peripheral parameters within both groups or between the groups (P>0.05). CONCLUSION: Although transient acute changes in central CV and HEX and peripheral ACA were observed, there is no significant effect on the long-term corneal health status when MMC is applied in a reasonable time range in LASEK surgery.

scholarly journals Comparison of Cornea Endothelial Cells Density the After Aspiration of Dense Cataracts using Two Variants of Phacoemulsification Parameters

2018 ◽  
Vol 15 (2S) ◽  
pp. 145-152
Author(s):  
S. V. Shukhaev ◽  
E. V. Boiko
Keyword(s):  
Endothelial Cells ◽  
Postoperative Complications ◽  
Cell Loss ◽  
Main Group ◽  
Cell Counting ◽  
Control Group ◽  
Corneal Endothelial Cells ◽  
Significant Difference ◽  
Ultrasound Energy ◽  
And Control

Purpose: to compare two types of phacoemulsification parameters (combination ultrasound and torsional US with IP) with estimating the number of postoperative complications caused by intraoperative trauma of corneal endothelial cells.Patients and methods. 72 patients underwent phacoemulsification with IOL implantation. Patients were randomly divided into two groups (main n = 33 and control n = 39). During the aspiration of lens fragments in the main group the combination ultrasound was used, while torsional US with IP was used in the control group. Endothelial cell counting and other examinations were performed 1 day, 1 week and 6 months after the surgery.Results. CDVA in the explored groups 1 week after the surgery was similar: the main group — 0.813 ± 0.228, the control group — 0.765 ± 0.250, There was also no statistically significant difference in the thickness of the cornea between the groups: the main group — 533.48 ± 12.41, the control group — 536.44 ± 10.92. At the same time, a statistically significant difference was found in the density of endothelial cells: the main group: 1871.30 ± 187.41 (after 1 week), 1865 ± 178.9 (after 6 months); control group: 1809.63 ± 225.43 (after 1 week), 1791 ± 230.82 (after 6 months). The percentage of cell loss was, respectively, lower in the main group at all times of observation: 3.90% (after 1 day), 4.54% (after 1 week), 4.9% (after 6 months). In the control group: 7.71% (after 1 day), 9.25% (after 1 week), 10.4% (after 6 months).Conclusions. Data obtained in this study has showed the advantages of combination ultrasound in comparison with torsional US with IP, when performing aspiration of dense lenses. Due to lower consumption of ultrasound energy and higher aspiration rate of the fragments, combination ultrasound can reduce the loss of corneal endothelial cells and decrease the number and severity of postoperative complications associated with it. 

scholarly journals Comparison of the Changes in Corneal Endothelial Cells after Pars Plana and Anterior Chamber Ahmed Valve Implant

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Ji Won Seo ◽  
Jong Yeon Lee ◽  
Dong Heun Nam ◽  
Dae Yeong Lee
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Anterior Chamber ◽  
Cell Damage ◽  
Corneal Endothelial Cells ◽  
Refractory Glaucoma ◽  
Endothelial Cell Damage ◽  
Significant Difference ◽  
Pars Plana

Purpose. To compare the changes in corneal endothelial cells after pars plana Ahmed glaucoma valve (AGV) implantation with those after the anterior chamber AGV implantation for refractory glaucoma.Methods. The medical records of 18 eyes with pars plana implantation of AGV (ppAGV) were reviewed retrospectively and were compared with 18 eyes with the anterior chamber AGV (acAGV) implant. The preoperative and postoperative endothelial cells, intraocular pressure (IOP), and postoperative complications during the follow-up in both groups were compared.Results. The average follow-up was 18 months. The postoperative endothelial cells in the ppAGV and acAGV groups were 2044 ± 303 and 1904 ± 324, respectively(P=0.25). The average percentage decrease in the endothelial cells in the ppAGV and acAGV groups at 18 months was 12.5% and 18.4%, respectively, and showed significant difference between the 2 groups(P=0.01). No difference in IOP control and the number of postoperative glaucoma medications was observed between the 2 groups.Conclusions. Endothelial cell damage in the ppAGV group for refractory glaucoma appeared to be lower than that in the acAGV group. Therefore, pars plana implantation of AGV may be preferred as it may have lower level of endothelial cell damage while maintaining similar level of IOP control.

scholarly journals DNA Cross-linking, Double-Strand Breaks, and Apoptosis in Corneal Endothelial Cells after a Single Exposure to Mitomycin C

2008 ◽  
Vol 49 (11) ◽  
pp. 4837 ◽  
Author(s):  
Danny S. Roh ◽  
Amanda L. Cook ◽  
Steven S. Rhee ◽  
Amar Joshi ◽  
Regis Kowalski ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Mitomycin C ◽  
Double Strand Breaks ◽  
Cross Linking ◽  
Corneal Endothelial Cells ◽  
Single Exposure ◽  
Strand Breaks

Bioengineering endothelialized neo-corneas using donor-derived corneal endothelial cells and decellularized corneal stroma

Biomaterials ◽  
2010 ◽  
Vol 31 (26) ◽  
pp. 6738-6745 ◽  
Author(s):  
Jin San Choi ◽  
James K. Williams ◽  
Margaret Greven ◽  
Keith A. Walter ◽  
Patrick W. Laber ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Corneal Stroma ◽  
Corneal Endothelial Cells

scholarly journals Changes in Corneal Endothelial Cells Morphology Due to Different Cataract Surgery Procedures: A Retrospective Study From November 2014 to November 2016

2020 ◽  
Vol 17 (2) ◽  
Author(s):  
Ma Bariah ◽  
Leong SY
Keyword(s):  
Endothelial Cells ◽  
Cataract Surgery ◽  
Refractive Error ◽  
Central Corneal Thickness ◽  
Corneal Thickness ◽  
Dissipated Energy ◽  
Corneal Endothelial Cells ◽  
Significant Difference ◽  
Duration Of Surgery ◽  
Snellen Chart

Introduction: The corneal endothelium is made up of an extremely thin layer of non -regenerating cells essential in keeping the cornea clear. The purpose of this study was to quantify the corneal endothelial cells morphology, visual acuity (VA) and refractive error in patients having femtosecond laser-assisted cataract surgery (FLACS) and conventional phacoemulsification surgery (CPS). Methods: Clinical data from 56 eyes were included in this study. 28 eyes had FLACS surgery and another 28 eyes had CPS. Distance VA, refractive error, corneal thickness and endothelial cells morphology were evaluated pre-operatively and at 1 month follow up. Refractive error was determined subjectively and VA was taken using Snellen chart. Specular microscope was used to measure endothelial cells morphology and central corneal thickness. Mann Whitney test and Spearman correlation analysis were used to analyse the results. Results: There were no statistically significant difference in the mean endothelial cell density (p=0.24), mean central corneal thickness (p=0.68), mean coefficient of variance (p=0.19), mean hexagonality (p=0.44), VA (p=0.72) and postoperative refractive outcome (p=0.66) between both groups. The duration of surgery was noted to have no statistically significant difference between both groups (p=0.08), while the cumulative dissipated energy and torsional time showed a significant difference between groups (p=0.01 and p<0.01 respectively). Conclusion: This study concluded that there was no significant difference in the endothelial cells morphology in patients undergoing both cataract surgeries. Both surgeries presented similar outcomes in the patients’ eyes.

Properties of Corneas Reconstructed with Cultured Human Corneal Endothelial Cells and Human Corneal Stroma

2005 ◽  
Vol 49 (6) ◽  
pp. 448-452 ◽  
Author(s):  
Shiro Amano ◽  
Tatsuya Mimura ◽  
Satoru Yamagami ◽  
Yasuhiro Osakabe ◽  
Kazunori Miyata
Keyword(s):  
Endothelial Cells ◽  
Corneal Stroma ◽  
Corneal Endothelial Cells ◽  
Human Corneal Endothelial Cells

scholarly journals Corneal endothelial cells changes in different stages of Keratoconus: a multi-Centre clinical study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ahmed Elmassry ◽  
Ahmed Osman ◽  
Moataz Sabry ◽  
Mohamed Elmassry ◽  
Mai Katkat ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Clinical Study ◽  
Cell Surface ◽  
Surface Area ◽  
Specular Microscopy ◽  
Care Hospital ◽  
Corneal Endothelial Cells ◽  
Cell Surface Area ◽  
Significant Difference ◽  
Stage 1

Abstract Purpose To assess the corneal endothelial cells morphology and count in keratoconus patients and their correlation with different stages of keratoconus. Methods Prospective non randomized multi-centric clinical study included 150 eyes of 150 keratoconus patients. Four centers in Egypt participated in this study included: Departments of Ophthalmology in Alexandria University, Tanta University and Port Said University and Alex I-Care hospital. Pentacam (Wavelight Oculyzer II) and specular microscopy (Tomey EM-3000) were done to all eyes. Keratoconic eyes were classified according to Amsler classification into stage 1, 2 and 3. Stage 1 included 99 eyes, stage 2 included 32 eyes & stage 3 included 19 eyes. Results The mean age of keratoconus patients was 24.07 ± 6.154 years. Forty five cases were males (30%) and 105 cases were females (70%). There was statistically significant difference in endothelial cell density (p < 0.001) and coefficient of variation (p = 0.012) between different stages of keratoconus eyes. Regarding cell surface area, there was statistically significant difference in cell surface area between different stages of keratoconus eyes (p < 0.001). In addition, for cell morphology, there was statistically significant difference between different stages of keratoconus eyes (p < 0.001). Conclusions Qualitative and quantitative structural changes were seen in endothelial cells of keratoconus eyes by using specular microscopy. For stages 1 and 2, keratoconus may not affect the corneal endothelim significantly. The endothelium in stage 3 shows significant changes regarding polymegathism and pleomorphism.

scholarly journals Effect of Mitomycin-C augmented trabeculectomy on corneal endothelial cells

2015 ◽  
Vol 10 (3) ◽  
pp. 257 ◽  
Author(s):  
Ghasem Fakhraie ◽  
Reza Zarei ◽  
Mohammad Zarei ◽  
Yadollah Eslami ◽  
Sasan Moghimi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Mitomycin C ◽  
Corneal Endothelial Cells
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