Study of the derivatives of 1-adamantane carboxylic acid to induced plateletaggregation human in vitro

The study of condition of the system of hemoglobin, release of peptide com- pounds, and proteolytic activity of human erythrocytes under conditions of hy- perglycemia of varying degrees is carried out. The increase in the level of hy- perglycemia is accompanied by a decrease of aﬃnity of hemoglobin to oxygen and the growth of level of metgemoglobin, the glycosylated hemoglobin level is increased in conditions of severe hyperglycemia. The level of formation of pep- tides in erythrocytes and the level of output of their cells is determined by the increase in trypsin-like activity in cytosol and increased membrane permeability of red blood cells.

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THE PRODUCTION OF PURPURA BY DERIVATIVES OF PNEUMOCOCCUS

Journal of Experimental Medicine ◽

10.1084/jem.43.1.111 ◽

1926 ◽

Vol 43 (1) ◽

pp. 111-106

Author(s):

Hobart A. Reimann ◽

Louis A. Julianelle

Keyword(s):

Red Blood Cells ◽

Hemolytic Activity ◽

Blood Cells ◽

Blood Platelets ◽

White Blood Cells ◽

Red Cells ◽

Severe Anemia ◽

Marked Diminution ◽

Derivatives Of

A study has been made of the variation in number of the blood platelets, and the red and white blood cells of white mice injected with pneumococcus extract. The blood platelets were greatly diminished after the injection, the greatest decrease usually occurring after 24 hours. Purpuric lesions usually developed when the number of blood platelets became less than 500,000 per c.mm. Regeneration of the platelets was accomplished by the 4th to the 9th day but there was an overregeneration and the return to normal did not take place until 2 weeks had elapsed. The red cells were also greatly reduced in number, but the rate of their destruction and regeneration was somewhat slower than that of the platelets. The leucocytes were slightly if at all influenced by the pneumococcus extract. Pneumococcus extracts were shown to be thrombolytic and hemolytic. Heat destroyed the activity of both the lysins in vitro. Heated extract produced purpura in mice but did not cause a severe anemia. Extracts adsorbed with either blood platelets or red blood cells showed a marked diminution in their thrombolytic and hemolytic activity in vitro. Such extracts, however, produced purpura as well as severe anemia and thrombopenia in mice.

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THE IN VITRO METABOLISM OF ERYTHROCYTES FROM NEWBORN INFANTS

Canadian Journal of Biochemistry and Physiology ◽

10.1139/y60-090 ◽

1960 ◽

Vol 38 (1) ◽

pp. 727-738 ◽

Cited By ~ 4

Author(s):

A. Zipursky ◽

T. LaRue ◽

L. G. Israels

Keyword(s):

Blood Cell ◽

Red Blood Cells ◽

Membrane Permeability ◽

Red Blood Cell ◽

Blood Cells ◽

Newborn Infants ◽

Phosphate Esters ◽

C Storage ◽

Rapid Fall

In the red blood cells of the newborn there is a rapid fall in non-hydrolyzable phosphate during in vitro incubation. This difference appears to be due to a decreased rate of synthesis of 2,3-diphosphoglyceric acid in the erythrocyte of the newborn. In addition the incorporation of P32 orthophosphate into the red blood cell is slower in the newborn than in the adult. During 4 °C storage of blood of adults and newborns there is a progressive fall in phosphate esters which is similar in both groups.The erythrocytes of the newborn contain more potassium and water than those of adults. During storage at 4 °C the cells of the newborn lose potassium more rapidly than those of the adult. This may be related to differences in membrane permeability.

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THE IN VITRO METABOLISM OF ERYTHROCYTES FROM NEWBORN INFANTS

Canadian Journal of Biochemistry and Physiology ◽

10.1139/o60-090 ◽

1960 ◽

Vol 38 (7) ◽

pp. 727-738 ◽

Cited By ~ 23

Author(s):

A. Zipursky ◽

T. LaRue ◽

L. G. Israels

Keyword(s):

Blood Cell ◽

Red Blood Cells ◽

Membrane Permeability ◽

Red Blood Cell ◽

Blood Cells ◽

Newborn Infants ◽

Phosphate Esters ◽

C Storage ◽

Rapid Fall

In the red blood cells of the newborn there is a rapid fall in non-hydrolyzable phosphate during in vitro incubation. This difference appears to be due to a decreased rate of synthesis of 2,3-diphosphoglyceric acid in the erythrocyte of the newborn. In addition the incorporation of P32 orthophosphate into the red blood cell is slower in the newborn than in the adult. During 4 °C storage of blood of adults and newborns there is a progressive fall in phosphate esters which is similar in both groups.The erythrocytes of the newborn contain more potassium and water than those of adults. During storage at 4 °C the cells of the newborn lose potassium more rapidly than those of the adult. This may be related to differences in membrane permeability.

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Ultrastructural observations on the in vitro cytoadherence of Plasmodium falciparum infected red blood cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100162132 ◽

1990 ◽

Vol 48 (3) ◽

pp. 914-915

Author(s):

D.J.P. Ferguson ◽

A.R. Berendt ◽

J. Tansey ◽

K. Marsh ◽

C.I. Newbold

Keyword(s):

Plasmodium Falciparum ◽

Red Blood Cells ◽

Blood Cells ◽

Umbilical Vein ◽

Cell Types ◽

Amelanotic Melanoma ◽

Cytoplasmic Process ◽

Umbilical Vein Endothelial Cells

In human malaria, the most serious clinical manifestation is cerebral malaria (CM) due to infection with Plasmodium falciparum. The pathology of CM is thought to relate to the fact that red blood cells containing mature forms of the parasite (PRBC) cytoadhere or sequester to post capillary venules of various tissues including the brain. This in vivo phenomenon has been studied in vitro by examining the cytoadherence of PRBCs to various cell types and purified proteins. To date, three Ijiost receptor molecules have been identified; CD36, ICAM-1 and thrombospondin. The specific changes in the PRBC membrane which mediate cytoadherence are less well understood, but they include the sub-membranous deposition of electron-dense material resulting in surface deformations called knobs. Knobs were thought to be essential for cytoadherence, lput recent work has shown that certain knob-negative (K-) lines can cytoadhere. In the present study, we have used electron microscopy to re-examine the interactions between K+ PRBCs and both C32 amelanotic melanoma cells and human umbilical vein endothelial cells (HUVEC).We confirm previous data demonstrating that C32 cells possess numerous microvilli which adhere to the PRBC, mainly via the knobs (Fig. 1). In contrast, the HUVEC were relatively smooth and the PRBCs appeared partially flattened onto the cell surface (Fig. 2). Furthermore, many of the PRBCs exhibited an invagination of the limiting membrane in the attachment zone, often containing a cytoplasmic process from the endothelial cell (Fig. 2).

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Effect of liposomal curcumin on red blood cells in vitro

Intrinsic Activity ◽

10.25006/ia.1.s1-a4.1 ◽

2013 ◽

Vol 1 (Suppl. 1) ◽

pp. A4.1

Author(s):

Angela Storka

Keyword(s):

Red Blood Cells ◽

Blood Cells

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THE SYNTHESIS OF PROTOPORPHYRIN IN VITRO BY RED BLOOD CELLS OF THE DUCK

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)51201-7 ◽

1950 ◽

Vol 183 (2) ◽

pp. 757-765 ◽

Cited By ~ 3

Author(s):

David Shemin ◽

Irving M. London ◽

D. Rittenberg

Keyword(s):

Red Blood Cells ◽

Blood Cells

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Novel Derivatives of 4-Methyl-1,2,3-Thiadiazole-5-Carboxylic Acid Hydrazide: Synthesis, Lipophilicity, and In Vitro Antimicrobial Activity Screening

Applied Sciences ◽

10.3390/app11031180 ◽

2021 ◽

Vol 11 (3) ◽

pp. 1180

Author(s):

Kinga Paruch ◽

Łukasz Popiołek ◽

Anna Biernasiuk ◽

Anna Berecka-Rycerz ◽

Anna Malm ◽

...

Keyword(s):

Antimicrobial Activity ◽

Carboxylic Acid ◽

Bacterial Infections ◽

Acid Hydrazide ◽

Antimicrobial Effect ◽

In Vitro Antimicrobial Activity ◽

Research Goal ◽

New Compounds ◽

Derivatives Of

Bacterial infections, especially those caused by strains resistant to commonly used antibiotics and chemotherapeutics, are still a current threat to public health. Therefore, the search for new molecules with potential antimicrobial activity is an important research goal. In this article, we present the synthesis and evaluation of the in vitro antimicrobial activity of a series of 15 new derivatives of 4-methyl-1,2,3-thiadiazole-5-carboxylic acid. The potential antimicrobial effect of the new compounds was observed mainly against Gram-positive bacteria. Compound 15, with the 5-nitro-2-furoyl moiety, showed the highest bioactivity: minimum inhibitory concentration (MIC) = 1.95–15.62 µg/mL and minimum bactericidal concentration (MBC)/MIC = 1–4 µg/mL.

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Nucleated red blood cells participate in myocardial regeneration in the toad Bufo Gargarizan Gargarizan

Experimental Biology and Medicine ◽

10.1177/15353702211013297 ◽

2021 ◽

pp. 153537022110132

Author(s):

Shu-Qin Liu ◽

Xiao-Ye Hou ◽

Feng Zhao ◽

Xiao-Ge Zhao

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Cardiac Injury ◽

Myocardial Regeneration ◽

Matrix Metalloproteinase 2 ◽

Enzyme Linked Immunosorbent Assay ◽

Cardiac Tissues ◽

Nucleated Red Blood Cells ◽

Model Animal

Heart regeneration is negligible in humans and mammals but remarkable in some ectotherms. Humans and mammals lack nucleated red blood cells (NRBCs), while ectotherms have sufficient NRBCs. This study used Bufo gargarizan gargarizan, a Chinese toad subspecies, as a model animal to verify our hypothesis that NRBCs participate in myocardial regeneration. NRBC infiltration into myocardium was seen in the healthy toad hearts. Heart needle-injury was used as an enlarged model of physiological cardiomyocyte loss. It recovered quickly and scarlessly. NRBC infiltration increased during the recovery. Transwell assay was done to in vitro explore effects of myocardial injury on NRBCs. In the transwell system, NRBCs could infiltrate into cardiac pieces and could transdifferentiate toward cardiomyocytes. Heart apex cautery caused approximately 5% of the ventricle to be injured to varying degrees. In the mildly to moderately injured regions, NRBC infiltration increased and myocardial regeneration started soon after the inflammatory response; the severely damaged region underwent inflammation, scarring, and vascularity before NRBC infiltration and myocardial regeneration, and recovered scarlessly in four months. NRBCs were seen in the newly formed myocardium. Enzyme-linked immunosorbent assay and Western blotting showed that the levels of tumor necrosis factor-α, interleukin- 1β, 6, and11, cardiotrophin-1, vascular endothelial growth factor, erythropoietin, matrix metalloproteinase- 2 and 9 in the serum and/or cardiac tissues fluctuated in different patterns during the cardiac injury-regeneration. Cardiotrophin-1 could induce toad NRBC transdifferentiation toward cardiomyocytes in vitro. Taken together, the results suggest that the NRBC is a cell source for cardiomyocyte renewal/regeneration in the toad; cardiomyocyte loss triggers a series of biological processes, facilitating NRBC infiltration and transition to cardiomyocytes. This finding may guide a new direction for improving human myocardial regeneration.

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Multimodal Diagnostics of Microrheologic Alterations in Blood of Coronary Heart Disease and Diabetic Patients

Diagnostics ◽

10.3390/diagnostics11010076 ◽

2021 ◽

Vol 11 (1) ◽

pp. 76

Author(s):

Anastasia Maslianitsyna ◽

Petr Ermolinskiy ◽

Andrei Lugovtsov ◽

Alexandra Pigurenko ◽

Maria Sasonko ◽

...

Keyword(s):

Coronary Heart Disease ◽

Heart Disease ◽

Red Blood Cells ◽

Blood Cells ◽

Optical Methods ◽

Diabetic Patients ◽

Aggregation Index ◽

Significant Difference

Coronary heart disease (CHD) has serious implications for human health and needs to be diagnosed as early as possible. In this article in vivo and in vitro optical methods are used to study blood properties related to the aggregation of red blood cells in patients with CHD and comorbidities such as type 2 diabetes mellitus (T2DM). The results show not only a significant difference of the aggregation in patients compared to healthy people, but also a correspondence between in vivo and in vitro parameters. Red blood cells aggregate in CHD patients faster and more numerously; in particular the aggregation index increases by 20 ± 7%. The presence of T2DM also significantly elevates aggregation in CHD patients. This work demonstrates multimodal diagnostics and monitoring of patients with socially significant pathologies.

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