Nucleated red blood cells participate in myocardial regeneration in the toad Bufo Gargarizan Gargarizan

Heart regeneration is negligible in humans and mammals but remarkable in some ectotherms. Humans and mammals lack nucleated red blood cells (NRBCs), while ectotherms have sufficient NRBCs. This study used Bufo gargarizan gargarizan, a Chinese toad subspecies, as a model animal to verify our hypothesis that NRBCs participate in myocardial regeneration. NRBC infiltration into myocardium was seen in the healthy toad hearts. Heart needle-injury was used as an enlarged model of physiological cardiomyocyte loss. It recovered quickly and scarlessly. NRBC infiltration increased during the recovery. Transwell assay was done to in vitro explore effects of myocardial injury on NRBCs. In the transwell system, NRBCs could infiltrate into cardiac pieces and could transdifferentiate toward cardiomyocytes. Heart apex cautery caused approximately 5% of the ventricle to be injured to varying degrees. In the mildly to moderately injured regions, NRBC infiltration increased and myocardial regeneration started soon after the inflammatory response; the severely damaged region underwent inflammation, scarring, and vascularity before NRBC infiltration and myocardial regeneration, and recovered scarlessly in four months. NRBCs were seen in the newly formed myocardium. Enzyme-linked immunosorbent assay and Western blotting showed that the levels of tumor necrosis factor-α, interleukin- 1β, 6, and11, cardiotrophin-1, vascular endothelial growth factor, erythropoietin, matrix metalloproteinase- 2 and 9 in the serum and/or cardiac tissues fluctuated in different patterns during the cardiac injury-regeneration. Cardiotrophin-1 could induce toad NRBC transdifferentiation toward cardiomyocytes in vitro. Taken together, the results suggest that the NRBC is a cell source for cardiomyocyte renewal/regeneration in the toad; cardiomyocyte loss triggers a series of biological processes, facilitating NRBC infiltration and transition to cardiomyocytes. This finding may guide a new direction for improving human myocardial regeneration.

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Direct measurements of the early erythropoietic effect of anemia

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1961.201.5.905 ◽

1961 ◽

Vol 201 (5) ◽

pp. 905-909 ◽

Cited By ~ 2

Author(s):

Allan J. Erslev ◽

Andre R. Boissevain

Keyword(s):

Bone Marrow ◽

Red Blood Cells ◽

Blood Cells ◽

Experimental Period ◽

Differential Count ◽

Early Effect ◽

Nucleated Red Blood Cells ◽

Direct Measurements ◽

Made In

Measurements were made in normal rabbits of the early effect of acute anemic anoxia on the level of erythropoietic factor and on erythropoietic tissue. The erythropoietic factor was found to be increased 3 hr after the onset of anemic anoxia and before measurable changes could be detected in the erythropoietic tissue. The subsequent production and release appeared to be directly proportional to time. Bone marrow changes during the 8-hr experimental period were more difficult to measure. The best technique appeared to be a simple differential count with the measurement of the ratio between early and late nucleated red blood cells. In vitro bone marrow incorporation of Fe59 could be used only if the bone marrow volumes studied were of identical size, a requirement difficult to fulfill. Plasma iron turnover was not sensitive enough to measure early bone marrow changes.

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Ultrastructural observations on the in vitro cytoadherence of Plasmodium falciparum infected red blood cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100162132 ◽

1990 ◽

Vol 48 (3) ◽

pp. 914-915

Author(s):

D.J.P. Ferguson ◽

A.R. Berendt ◽

J. Tansey ◽

K. Marsh ◽

C.I. Newbold

Keyword(s):

Plasmodium Falciparum ◽

Red Blood Cells ◽

Blood Cells ◽

Umbilical Vein ◽

Cell Types ◽

Amelanotic Melanoma ◽

Cytoplasmic Process ◽

Umbilical Vein Endothelial Cells

In human malaria, the most serious clinical manifestation is cerebral malaria (CM) due to infection with Plasmodium falciparum. The pathology of CM is thought to relate to the fact that red blood cells containing mature forms of the parasite (PRBC) cytoadhere or sequester to post capillary venules of various tissues including the brain. This in vivo phenomenon has been studied in vitro by examining the cytoadherence of PRBCs to various cell types and purified proteins. To date, three Ijiost receptor molecules have been identified; CD36, ICAM-1 and thrombospondin. The specific changes in the PRBC membrane which mediate cytoadherence are less well understood, but they include the sub-membranous deposition of electron-dense material resulting in surface deformations called knobs. Knobs were thought to be essential for cytoadherence, lput recent work has shown that certain knob-negative (K-) lines can cytoadhere. In the present study, we have used electron microscopy to re-examine the interactions between K+ PRBCs and both C32 amelanotic melanoma cells and human umbilical vein endothelial cells (HUVEC).We confirm previous data demonstrating that C32 cells possess numerous microvilli which adhere to the PRBC, mainly via the knobs (Fig. 1). In contrast, the HUVEC were relatively smooth and the PRBCs appeared partially flattened onto the cell surface (Fig. 2). Furthermore, many of the PRBCs exhibited an invagination of the limiting membrane in the attachment zone, often containing a cytoplasmic process from the endothelial cell (Fig. 2).

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Effect of liposomal curcumin on red blood cells in vitro

Intrinsic Activity ◽

10.25006/ia.1.s1-a4.1 ◽

2013 ◽

Vol 1 (Suppl. 1) ◽

pp. A4.1

Author(s):

Angela Storka

Keyword(s):

Red Blood Cells ◽

Blood Cells

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Nucleated red blood cells and hematological scoring system – Future trends in early onset neonatal sepsis

Medpusle International Journal of Pathology ◽

10.26611/10512112 ◽

2019 ◽

Vol 12 (1) ◽

pp. 53-58

Author(s):

Shivendra Vikram Singh ◽

◽

Megalamane Supreetha ◽

Satyavathi R Alva ◽

◽

...

Keyword(s):

Red Blood Cells ◽

Neonatal Sepsis ◽

Blood Cells ◽

Early Onset ◽

Scoring System ◽

Future Trends ◽

Nucleated Red Blood Cells

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Nucleated Red Blood Cells as Markers of Perinatal Adaptation in Preterm Neonates Receiving Minimally Invasive Surfactant Therapy

American Journal of Perinatology ◽

10.1055/s-0041-1726317 ◽

2021 ◽

Author(s):

Itamar Nitzan ◽

Calum T. Roberts ◽

Risha Bhatia ◽

Francis B. Mimouni ◽

Arvind Sehgal

Keyword(s):

Minimally Invasive ◽

Right Ventricular ◽

Red Blood Cells ◽

Blood Cells ◽

Surfactant Therapy ◽

Preterm Neonates ◽

Oxygen Requirement ◽

Nucleated Red Blood Cells ◽

Intrauterine Hypoxia ◽

Ventricular Ejection Time

Objective The study aimed to assess the association of nucleated red blood cells (NRBC), a surrogate of intrauterine hypoxia, and elevated pulmonic vascular resistance (E-PVR) and oxygen requirement after minimally invasive surfactant therapy (MIST). Study Design Retrospective study of a cohort of preterm neonates that received MIST in a single unit. Results NRBC were measured in 65 of 75 (87%) neonates administered MIST during the period. In total, 22 of 65 (34%) infants had pre-MIST echocardiography (ECHO).Neonates with elevated NRBC (predefined as >5 × 109/L, n = 16) required higher post-MIST fraction of inspired oxygen (FiO2) than neonates with normal NRBC (<1 × 109/L, n = 17; FiO2 = 0.31 ± 0.10 and 0.24 ± 0.04, respectively, p = 0.02).NRBC correlated positively with % of time in right to left ductal shunt (r = 0.51, p = 0.052) and inversely with right ventricular stroke volume (r = −0.55, p = 0.031) and time to peak velocity to right ventricular ejection time ratio (r = −0.62, p < 0.001). Conclusion Elevated NRBC are associated with elevated FiO2 after MIST and elevated E-PVR. Intrauterine hypoxia may impact postnatal circulatory adaptations and oxygen requirement. Key Points

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THE SYNTHESIS OF PROTOPORPHYRIN IN VITRO BY RED BLOOD CELLS OF THE DUCK

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)51201-7 ◽

1950 ◽

Vol 183 (2) ◽

pp. 757-765 ◽

Cited By ~ 3

Author(s):

David Shemin ◽

Irving M. London ◽

D. Rittenberg

Keyword(s):

Red Blood Cells ◽

Blood Cells

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Multimodal Diagnostics of Microrheologic Alterations in Blood of Coronary Heart Disease and Diabetic Patients

Diagnostics ◽

10.3390/diagnostics11010076 ◽

2021 ◽

Vol 11 (1) ◽

pp. 76

Author(s):

Anastasia Maslianitsyna ◽

Petr Ermolinskiy ◽

Andrei Lugovtsov ◽

Alexandra Pigurenko ◽

Maria Sasonko ◽

...

Keyword(s):

Coronary Heart Disease ◽

Heart Disease ◽

Red Blood Cells ◽

Blood Cells ◽

Optical Methods ◽

Diabetic Patients ◽

Aggregation Index ◽

Significant Difference

Coronary heart disease (CHD) has serious implications for human health and needs to be diagnosed as early as possible. In this article in vivo and in vitro optical methods are used to study blood properties related to the aggregation of red blood cells in patients with CHD and comorbidities such as type 2 diabetes mellitus (T2DM). The results show not only a significant difference of the aggregation in patients compared to healthy people, but also a correspondence between in vivo and in vitro parameters. Red blood cells aggregate in CHD patients faster and more numerously; in particular the aggregation index increases by 20 ± 7%. The presence of T2DM also significantly elevates aggregation in CHD patients. This work demonstrates multimodal diagnostics and monitoring of patients with socially significant pathologies.

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In vitro characterization of engineered red blood cells as viral traps against HIV-1 and SARS-CoV-2

Molecular Therapy — Methods & Clinical Development ◽

10.1016/j.omtm.2021.03.003 ◽

2021 ◽

Vol 21 ◽

pp. 161-170 ◽

Cited By ~ 1

Author(s):

Magnus A.G. Hoffmann ◽

Collin Kieffer ◽

Pamela J. Bjorkman

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

In Vitro Characterization ◽

Hiv 1

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In Vitro Assay for Single-cell Characterization of Impaired Deformability in Red Blood Cells under Recurrent Episodes of Hypoxia

Lab on a Chip ◽

10.1039/d1lc00598g ◽

2021 ◽

Author(s):

YUHAO QIANG ◽

Jia Liu ◽

Ming Dao ◽

E Du

Keyword(s):

Shear Stress ◽

Red Blood Cells ◽

Single Cell ◽

Blood Cells ◽

Blood Circulation ◽

In Vitro Assay ◽

Vitro Assay ◽

Cyclic Shear

Red blood cells (RBCs) are subjected to recurrent changes in shear stress and oxygen tension during blood circulation. The cyclic shear stress has been identified as an important factor that...

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