scholarly journals Tissue Optical Clearing in the Ultraviolet for Clinical Use in Dentistry to Optimize the Treatment of Chronic Recurrent Aphthous Stomatitis

2020 ◽  
pp. 040301
Author(s):  
Alexey A. Selifonov ◽  
Valery V. Tuchin
Keyword(s):  
Uv Spectroscopy ◽  
Biological Tissues ◽  
Recurrent Aphthous Stomatitis ◽  
Glycerol Solution ◽  
Strong Light ◽  
Optical Clearing ◽  
Index Matching ◽  
Refractive Index Matching ◽  
Tissue Optical Clearing ◽  
Uv Range

The immersion optical clearing (OC) treatment with a highly concentrated glycerol solution has induced three new tissue windows in the UV spectral range of gingival tissues – from 200 to 250 nm, from 250 to 300 nm and from 300 to 400 nm. By combining the immersion OC technique in human tissues with UV-spectroscopy, it was possible to verify and study the major OC mechanisms – tissue dehydration and refractive index matching, and that the OC efficiency is higher in the deep-UV than in the visible-NIR range. Since all biological tissues present high scattering in the UV range, the presented technology, which basically reduces the strong light scattering in the UV range, has a broad application area in medicine. The effectiveness of the developed technology combining UV phototherapy and OC in application to treatment of aphthous recurrent stomatitis in children was demonstrated.

scholarly journals Refractive index matching applied to fecal smear clearing

2005 ◽  
Vol 47 (6) ◽  
pp. 347-350 ◽  
Author(s):  
Cláudio S. Ferreira
Keyword(s):  
Refractive Index ◽  
Sucrose Solution ◽  
Glycerol Solution ◽  
Human Feces ◽  
Microscope Slide ◽  
Helminth Eggs ◽  
Index Matching ◽  
Refractive Index Matching ◽  
Fecal Smear ◽  
Effect Of Light

Thick smears of human feces can be made adequate for identification of helminth eggs by means of refractive index matching. Although this effect can be obtained by simply spreading a fleck of feces on a microscope slide, a glycerol solution has been routinely used to this end. Aiming at practicability, a new quantitative technique has been developed. To enhance both sharpness and contrast of the images, a sucrose solution (refractive index = 1.49) is used, which reduces the effect of light-scattering particulates. To each slide a template-measured (38.5 mm³) fecal sample is transferred. Thus, egg counts and sensitivity evaluations are easily made.

scholarly journals Rapid single-wavelength lightsheet localization microscopy for clarified tissue

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Li-An Chu ◽  
Chieh-Han Lu ◽  
Shun-Min Yang ◽  
Yen-Ting Liu ◽  
Kuan-Lin Feng ◽  
...  
Keyword(s):  
Single Molecule ◽  
Super Resolution ◽  
Biological Tissues ◽  
Adult Brain ◽  
Imaging Data ◽  
Transporter Protein ◽  
Index Matching ◽  
Refractive Index Matching ◽  
Nanoscale Imaging ◽  
Resolution Imaging

Abstract Optical super-resolution microscopy allows nanoscale imaging of protein molecules in intact biological tissues. However, it is still challenging to perform large volume super-resolution imaging for entire animal organs. Here we develop a single-wavelength Bessel lightsheet method, optimized for refractive-index matching with clarified specimens to overcome the aberrations encountered in imaging thick tissues. Using spontaneous blinking fluorophores to label proteins of interest, we resolve the morphology of most, if not all, dopaminergic neurons in the whole adult brain (3.64 × 107 µm3) of Drosophila melanogaster at the nanometer scale with high imaging speed (436 µm3 per second) for localization. Quantitative single-molecule localization reveals the subcellular distribution of a monoamine transporter protein in the axons of a single, identified serotonergic Dorsal Paired Medial (DPM) neuron. Large datasets are obtained from imaging one brain per day to provide a robust statistical analysis of these imaging data.

QUANTITATIVE CONTROL OF OPTICAL CLEARING EFFECTS STUDIED WITH TISSUE-LIKE PHANTOM

2010 ◽  
Vol 03 (03) ◽  
pp. 195-202 ◽  
Author(s):  
JINGYING JIANG ◽  
WEI CHEN ◽  
QILIANG GONG ◽  
KEXIN XU
Keyword(s):  
Optical Properties ◽  
Biological Tissues ◽  
Porcine Skin ◽  
Tissue Samples ◽  
Optical Clearing ◽  
Tissue Optical Properties ◽  
Application Potential ◽  
The Difference ◽  
Tissue Optical Clearing

Tissue optical clearing by use of optical clearing agents (OCAs) has been proven to have potential to reduce the highly scattering effect of biological tissues in optical techniques. However, the difference in tissue samples could lead to unreliable results, making it difficult to quantitatively control the dose of OCAs during the course of tissue optical clearing. In this work, in order to study the effects of optical clearing, we customized tissue-like phantoms with optical properties of some biological tissue. Diffuse reflectance and total transmittance of tissue-like phantoms with different OCAs (DMSO or glycerol) and porcine skin tissues were measured. Then optical property parameters were calculated by inverse adding-doubling (IAD) algorithm. Results showed that OCAs could lead to a reduction in scattering of tissue-like phantoms as it did to porcine skin tissue in vitro. Furthermore, a series of relational expressions could be fit to quantitatively describe the relationship between the doses of OCAs and the reduction of scattering effects. Therefore, proper tissue-like phantom could facilitate optical clearing to be used in quantitative control of tissue optical properties, and further promote the application potential of optical clearing to light-based noninvasive diagnostic and therapeutic techniques.

scholarly journals PARAMETRIC STUDY OF TISSUE OPTICAL CLEARING BY LOCALIZED MECHANICAL COMPRESSION USING COMBINED FINITE ELEMENT AND MONTE CARLO SIMULATION

2010 ◽  
Vol 03 (03) ◽  
pp. 203-211 ◽  
Author(s):  
WILLIAM C. VOGT ◽  
HAIOU SHEN ◽  
GE WANG ◽  
CHRISTOPHER G. RYLANDER
Keyword(s):  
Finite Element ◽  
Monte Carlo ◽  
Optical Properties ◽  
Light Transmission ◽  
Compressive Strain ◽  
Monte Carlo Model ◽  
Biological Tissues ◽  
Optical Clearing ◽  
Tissue Optical Properties ◽  
Tissue Optical Clearing

Tissue Optical Clearing Devices (TOCDs) have been shown to increase light transmission through mechanically compressed regions of naturally turbid biological tissues. We hypothesize that zones of high compressive strain induced by TOCD pins produce localized water displacement and reversible changes in tissue optical properties. In this paper, we demonstrate a novel combined mechanical finite element model and optical Monte Carlo model which simulates TOCD pin compression of an ex vivo porcine skin sample and modified spatial photon fluence distributions within the tissue. Results of this simulation qualitatively suggest that light transmission through the skin can be significantly affected by changes in compressed tissue geometry as well as concurrent changes in tissue optical properties. The development of a comprehensive multi-domain model of TOCD application to tissues such as skin could ultimately be used as a framework for optimizing future design of TOCDs.

scholarly journals A tunable refractive index matching medium for live imaging cells, tissues and model organisms

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Tobias Boothe ◽  
Lennart Hilbert ◽  
Michael Heide ◽  
Lea Berninger ◽  
Wieland B Huttner ◽  
...  
Keyword(s):  
Refractive Index ◽  
Image Quality ◽  
Light Microscopy ◽  
Penetration Depth ◽  
Cell Cultures ◽  
Model Organisms ◽  
Optical Clearing ◽  
Index Matching ◽  
Refractive Index Matching ◽  
Tunable Refractive Index

In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus substantially improves image quality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral organoids.

scholarly journals Toward Better Medical Diagnosis: Tissue Optical Clearing

2020 ◽  
Vol 2 (1) ◽  
pp. 13-21
Author(s):  
Omnia Hamdy ◽  
Rania M. Abdelazeem
Keyword(s):  
Refractive Index ◽  
Medical Diagnosis ◽  
Imaging Techniques ◽  
High Refractive Index ◽  
Biological Tissues ◽  
Optical Clearing ◽  
Research Areas ◽  
Body Tissues ◽  
Deep Imaging ◽  
Tissue Optical Clearing

Reaching efficient, safe and painless medical diagnosis procedure is a very valued goal for many research areas. Despite the great advantages of using optical imaging techniques in medical diagnosis including high safety and relative simplicity, it still suffers from relatively low resolution and penetration depth in the multiple scattering mediums such as biological tissues. Therefore, researchers began to devise ways to reduce the scattering properties of the tissue, hence increasing the imaging contrast. Optical clearing concept is introduced to do this job. This technique can reduce tissues scattering properties by using high refractive index chemicals, thus making the tissue transparent by equalizing the refractive index through that medium. In this paper, theory and techniques of optical clearing method are illustrated utilizing its benefits for deep imaging of different body tissues and organs.

scholarly journals ASSESSMENT OF TISSUE OPTICAL CLEARING AS A FUNCTION OF GLUCOSE CONCENTRATION USING OPTICAL COHERENCE TOMOGRAPHY

2010 ◽  
Vol 03 (03) ◽  
pp. 169-176 ◽  
Author(s):  
NARENDRAN SUDHEENDRAN ◽  
MOHAMED MOHAMED ◽  
MOHAMAD G. GHOSN ◽  
VALERY V. TUCHIN ◽  
KIRILL V. LARIN
Keyword(s):  
Optical Coherence Tomography ◽  
Glucose Concentration ◽  
Glucose Solution ◽  
Tissue Sample ◽  
Biological Tissues ◽  
Optical Coherence ◽  
Porcine Skin ◽  
Optical Clearing ◽  
Reflected Light ◽  
Tissue Optical Clearing

One of the major challenges in imaging biological tissues using optical techniques, such as optical coherence tomography (OCT), is the lack of light penetration due to highly turbid structures within the tissue. Optical clearing techniques enable the biological samples to be more optically homogeneous, allowing for deeper penetration of light into the tissue. This study investigates the effect of optical clearing utilizing various concentrations of glucose solution (10%, 30%, and 50%) on porcine skin. A gold-plated mirror was imaged beneath the tissue and percentage clearing was determined by monitoring the change in reflected light intensity from the mirror over time. The ratio of percentage clearing per tissue thickness for 10%, 30% and 50% glucose was determined to be 4.7 ±1.6% mm-1 (n = 6), 10.6 ±2.0% mm-1 (n = 7) and 21.8 ±2.2% mm-1 (n = 5), respectively. It was concluded that while higher glucose concentration has the highest optical clearing effect, a suitable concentration should be chosen for the purpose of clearing, considering the osmotic stress on the tissue sample.

scholarly journals Scalable cytoarchitectonic characterization of large intact human neocortex samples

2018 ◽  
Author(s):  
Sven Hildebrand ◽  
Anna Schueth ◽  
Andreas Herrler ◽  
Ralf Galuske ◽  
Alard Roebroeck
Keyword(s):  
Refractive Index ◽  
Low Cost ◽  
Adult Brain ◽  
Human Cortex ◽  
Optical Clearing ◽  
Volume Imaging ◽  
Index Matching ◽  
Refractive Index Matching ◽  
Formalin Fixed

AbstractWe describe MASH (Multiscale Architectonic Staining of Human cortex): a simple, fast and low-cost cytoarchitectonic labeling and optical clearing approach for human cortex samples, which can be applied to large formalin fixed adult brain samples. A suite of small-molecule fluorescent nuclear and cytoplasmic dyes in combination with new refractive index matching solutions allows deep volume imaging. This enables highly scalable human neocortical cytoarchitecture characterization with a large 3D scope.

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