scholarly journals Hyperspectral quantitative models for Chlorophyll-a of Chlorella vulgaris and Microcystis aeruginosa

2007 ◽  
Vol 19 (3) ◽  
pp. 261-268
Author(s):  
GAO Yan ◽  
◽  
ZHOU Feng ◽  
ZHANG Shucai ◽  
ZHANG Wei ◽  
...  
Keyword(s):  
Chlorophyll A ◽  
Microcystis Aeruginosa ◽  
Chlorella Vulgaris ◽  
Quantitative Models

scholarly journals Identification and antialgal properties of 
o-coumaric acid isolated from Eupatorium fortune Turcz

2018 ◽  
Vol 9 (4) ◽  
pp. 228-234
Author(s):  
Thanh Nga Pham ◽  
Huu Dien Pham ◽  
Thi Phuong Quynh Le ◽  
Tien Dat Nguyen ◽  
Thi Thuy Duong ◽  
...  
Keyword(s):  
Chlorophyll A ◽  
Microcystis Aeruginosa ◽  
Chlorella Vulgaris ◽  
Antibacterial Properties ◽  
Common Species ◽  
Inhibitory Effect ◽  
Coumaric Acid ◽  
Toxic Cyanobacteria ◽  
Growth Inhibitory ◽  
Ethanol Extracts

In our pervious study, the ethanol extracts from Eupatorium fortunei Turcz at concentrations of 200 ÷ 500 mg L-1 significantly inhibited the growth of Microcystis aeruginosa, which is the most common species, responsible for toxic cyanobacteria blooming in fresh water. o-Coumaric acid (or 2-hydroxy-cinnamic acid) isolated from E. fortunei was tested its growth-inhibitory effect on M. aeruginosa and Chlorella vulgaris at the concentrations of 1.0, 10.0 and 100.0 mg L-1 in the 96- hour experiment by the optical density and the analytical method of chlorophyll a concentration. Results indicated that the compound strongly affected towards M. aeruginosa at the concentration of 100.0 mg L-1 with the inhibition efficiency (IE) values of 76.76 % and 84.66 %, respectively while those for C. vulgaris were lower just of 60.59%, and 74.53 %, respectively. The obtained data demonstrated that two methods were highly consistent and o-coumaric acid was more toxic to M. aeruginosa than C. vulgaris at all tested concentrations (p<0.05). The images of M. aeruginosa and C. vulgaris cells under the light microscope clearly showed the damage of these cells under the attck of o-coumaric acid. Although o-coumaric compound was widely demonstrated antibacterial properties in previous reports, to the best of our knowledge, our study was the first report about effect of o-coumaric acid on the growth of M. aeruginosa and C. vulgaris. Những nghiên cứu trước đây đã chỉ ra rằng cao chiết etanol từ cây Mần tưới Eupatorium fortune Turcz tại dải nồng độ 200 ÷ 500 µg mL-1 ức chế mạnh sinh trưởng của Microcystis aeruginosa, - loài phổ biến nhất gây nên sự bùng nổ tảo độc trong hệ sinh thái nước ngọt. o-Coumaric axit (hay 2-hydroxy-cinnamic axit) phân lập từ E. fortune được tiến hành đánh giá ảnh hưởng lên sinh trưởng của hai loài M. aeruginosa và Chlorella vulgaris tại ba nồng độ là 1.0, 10.0 and 100.0 mg L-1 trong thời gian 96 giờ thực nghiệm theo phương pháp đo mật độ quang và phân tích hàm lượng chlorophyll a. Kết quả nghiên cứu cho thấy sau 96 giờ phơi nhiễm tại nồng độ 100 mg L-1 hoạt chất ức chế mạnh tới M. aeruginosa với giá trị ức chế sinh trưởng (IE) tương ứng là 76.76 và 84.66%. Giá trị IE đối với C. vulgaris ghi nhận thấp hơn chỉ là 60.65 và 74.53%, tương ứng. Hai phương pháp phân tích trên có tính nhất quán cao và o-coumaric ức chế sinh trưởng lên loài M. aeruginosa mạnh hơn so với loài C. vulgaris tại tất cả các nồng độ nghiên cứu (p<0.05). Ảnh chụp các tế bào M. aeruginosa và C. vulgaris dưới kính hiển vi điện tử đã chứng minh những tổn thương của tế bào dưới tác động của o-coumaric axit. Mặc dù o-coumaric cho thấy đặc tính chống khuẩn cao trong các công bố trước đây, nhưng theo hiểu biết của chúng tôi đây là nghiên cứu đầu tiên công bố về ảnh hưởng của hoạt chất o-coumaric axit lên sinh trưởng của M. aeruginosa và C. vulgaris.

scholarly journals PHYSIOLOGICAL AND BIOCHEMICAL CHANGES IN ALGAL CULTURES OF CHLORELLA VULGARIS AND SYNECHOCYSTIS SALINA (MESOPHILIC AND ANTARCTIC ISOLATES) OCCURING AFTER TREATMENT WITH UV-B RADIATION

2017 ◽  
pp. 73-82
Author(s):  
Dilyana Doneva ◽  
Juliana Ivanova ◽  
Lyudmila Kabaivanova
Keyword(s):  
Oxidative Stress ◽  
Chlorophyll A ◽  
Chlorella Vulgaris ◽  
Markers Of Oxidative Stress ◽  
Protective Strategies ◽  
The Antarctic ◽  
Antarctic Algae ◽  
Β Carotene ◽  
Stimulation Of

Determination of biomass production and viability of algal cells of Chlorella vulgaris and Synechocystis salina exposed to UV-B radiation were carried out in this study together with comparison of the mesophilic and antarctic isolates of both investigated strains. Estimation of the content of the pigments: chlorophyll a, chlorophyll b, β-carotene, C-phycocyanin and allo-phycocyanin in algal cells exposed to UV-B radiation was also accomplished. The obtained results showed that the antarctic algae are more resistant to oxidative stress than their mesophilic counterparts. The antarctic isolates of Ch. vulgaris and S. salina compared with the mesophilic ones - up to 72 h showed tolerance to low exposures of radiation, expressed in a slight stimulation of growth and viability of the cells. Antarctic isolates also showed greater resistance to low doses of UV-B radiation manifested by stimulation of the synthesis of chlorophyll a and β-carotene. The registered increase in the amount of C- and allo-phycocyanin in antarctic isolates of S. salina showed that they had developed protective strategies against UV-B radiation by increasing the concentration of the phycobiliproteins. As a result of increased UV-B background, in antarctic isolates, stronger antioxidant defence mechanisms are triggered, which proved the possibility of using them as markers of oxidative stress.

Inhibitory effects of golden thread (Coptis chinensis) and berberine on Microcystis aeruginosa

2010 ◽  
Vol 61 (3) ◽  
pp. 763-769 ◽  
Author(s):  
Shulin Zhang ◽  
Bo Zhang ◽  
Kezhi Xing ◽  
Xiumei Zhang ◽  
Xiuping Tian ◽  
...  
Keyword(s):  
Protein Content ◽  
Chlorophyll A ◽  
Cell Density ◽  
Microcystis Aeruginosa ◽  
Carbohydrate Content ◽  
Chinese Herbs ◽  
Inhibitory Effects ◽  
Coptis Chinensis ◽  
Golden Thread ◽  
Chlorophyll A Content

The effects of 40 Chinese herbs on Microcystis aeruginosa growth were monitored spectrophotometrically. Golden thread (Coptis chinensis) exhibited the best inhibitory effects. Cell density of M. aeruginosa decreased with the increasing concentrations of golden thread and the prolongation of exposure time. Decreases in protein content, carbohydrate content, and chlorophyll a content were observed in a golden thread concentration-dependent manner after 96 h exposure. Changes in cell density, protein content, carbohydrate content, and chlorophyll a content of M. aeruginosa exposed to berberine, the main component of golden thread, were also investigated. It was observed that berberine exhibited the same inhibitory effects on M. aeruginosa. The results suggested that golden thread could inhibit M. aeruginosas growth effectively, and berberine might be the main allelochemical implementing the inhibitory effects of golden thread.

Influence of Molecular Oxygen on the Chlorophyll Fluorescence Decay of Green Algae

1999 ◽  
Vol 54 (5-6) ◽  
pp. 348-352 ◽  
Author(s):  
Silke Oellerich ◽  
Daniel Berg ◽  
Karlheinz Maier ◽  
Frank Terjung
Keyword(s):  
Chlorophyll Fluorescence ◽  
Molecular Oxygen ◽  
Chlorophyll A ◽  
Green Algae ◽  
Chlorella Vulgaris ◽  
Fluorescence Decay ◽  
Singlet Excited State ◽  
Chlorophyll Fluorescence Quenching ◽  
Photosynthetic Organisms ◽  
Quenching By Oxygen

Abstract Chlorella vulgaris, Chlorophyll Fluorescence Quenching, Green Algae, Molecular Oxygen Molecular oxygen can act as a collisional quencher of the singlet excited state of chlorophyll a. This effect is well described for chlorophyll a in various solvents but not for chlorophyll a in the antenna complexes of photosynthetic organisms. We studied the chlorophyll fluorescence decay of Chlorella vulgaris cells under different oxygen concentrations but did not find any evidence for quenching by oxygen.

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