scholarly journals Seasonal Variations of Lipid Content and Composition in Starfish Asterias amurensis Lütken

2012 ◽  
Vol 15 (1) ◽  
pp. 45 ◽  
Author(s):  
A.K.M. Azad Shah ◽  
H. Kurihara ◽  
K. Takahashi
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Lipid Content ◽  
Polar Lipid ◽  
Lipid Fraction ◽  
Total Lipid Content ◽  
Polar Lipid Fraction ◽  
Internal Organs ◽  
Asterias Amurensis ◽  
Lipid Fractions

The total lipid content, lipid classes and fatty acid composition in the internal organs of starfish (Asterias amurensis) were analyzed to determine the effects of seasons (winter and spring). The non-polar and polar lipid fractions obtained from starfish internal organs were analyzed through two seasons using thin layer chromatography (TLC) and gas liquid chromatography. Total lipid content of internal organs was 10.18% in spring and 8.21% in winter as wet weight basis. The predominant lipid class in spring was triglyceride whereas free fatty acids were the main lipid class in winter. The most abundant fatty acid of non-polar lipid fraction was eicosamonoenoic acid (C20:1) in spring having the highest proportion (29.2% of total fatty acid) as compared to the winter. Eicosapentaenoic acid (EPA) was also found significantly (P < 0.05) higher in spring compared to winter in the non-polar lipid fraction. On the other hand, comparatively lower amount of EPA was observed in spring than winter in the polar lipid fraction. Proportions of other fatty acids in non-polar and polar lipid fractions were also varied seasonally. This result might be useful for commercial production of lipid from internal organ of starfish with a view to potential use in food, pharmaceutical, cosmetics and other non-food industries.

Neutral lipids, phospholipids, and a betaine lipid of the snow mold fungus Microdochium nivale

1998 ◽  
Vol 44 (11) ◽  
pp. 1051-1059 ◽  
Author(s):  
Anita Istokovics ◽  
Naoki Morita ◽  
Kazuo Izumi ◽  
Tamotsu Hoshino ◽  
Isao Yumoto ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Neutral Lipid ◽  
Polar Lipid ◽  
Lipid Fraction ◽  
Microdochium Nivale ◽  
Neutral Lipid Fraction ◽  
Polar Lipid Fraction ◽  
Snow Mold ◽  
Betaine Lipid

The hyphae of the snow mold Microdochium nivale contained lipids in a yield of about 10% w/w of the dry matter of hyphae. The total lipid was fractionated into neutral and polar lipid fractions. In the neutral lipid fraction, triacylglylcerol was the sole major component. As minor components, ergosterol, diacylglycerol, free fatty acid, and fatty acyl ergosterol were identified. The polar lipid fraction contained phospholipids, glycolipids, and a lipid containing neither phosphorus nor sugar. Phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, phosphatidylserine, and phosphatidic acid were identified as phospholipids. The polar lipid fraction included at least four kinds of glycolipids that have not been identified. A very unusual lipid in fungi, a betaine lipid, diacylglyceryltrimethylhomoserine, was identified by chemical and physicochemical analyses. The level of the neutral lipid fraction, which accounted for 60% of the total lipid in hyphae at the exponential phase, was significantly increased compared with that of the polar lipid fraction and constituted 80% of the total at the stationary phase. The neutral and polar lipids of Microdochium nivale contained 18:3 (9,12,15), 18:2 (9,12), 18:1 (9), and 16:0 as principal fatty acids. Among them, 18:2 (9,12) and 18:3 (9,12,15) were the major fatty acids of triacylglycerol, phosphatidylcholine, phosphatidylglycerol, and phosphatidylethanolamine, whereas in diacylglyceryltrimethylhomoserine, the major components were 16:0 and 18:3 (9,12,15).Key words: snow mold, phospholipids, betaine lipid, fatty acid, Microdochium nivale.

The fatty acid composition of conidia of Aspergillus niger V. Tiegh.

1972 ◽  
Vol 18 (10) ◽  
pp. 1575-1576 ◽  
Author(s):  
M. Gunasekaran ◽  
W. M. Hess ◽  
D. J. Weber
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Aspergillus Niger ◽  
Lipid Content ◽  
Polar Lipid ◽  
Lipid Fraction ◽  
Lipid Bodies ◽  
Neutral Lipid Fraction ◽  
Total Fatty Acids

The lipids from conidia of Aspergillus niger were extracted and identified. The polar lipid content was higher (54.3%) than the neutral lipid fraction (45.7%). The major fatty acids present were C16:0, C18:0, C18:1, and C18:2. The amount of unsaturation in total fatty acids (62%) was higher than the unsaturation in the polar lipids (35%). Freeze-etched replicas of dormant conidia revealed the presence of lipid bodies.

scholarly journals Fatty Acids Profiles of Stipe and Blade from the Norwegian Brown Macroalga Laminaria hyperborea, Using Off-Line SPE and GC-MS

2016 ◽  
Author(s):  
Lena Oksdøl Foseid ◽  
Hanne Devle ◽  
Yngve Stenstrøm ◽  
Carl Fredrik Naess-Andresen ◽  
Dag Ekeberg
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Neutral Lipids ◽  
Methyl Esters ◽  
Lipid Fraction ◽  
Environmental Benefits ◽  
Laminaria Hyperborea ◽  
Alpha Linolenic Acid ◽  
Lipid Fractions

A thorough analysis and comparison of the fatty acid profiles of stipe and blade from Laminaria hyperborea, a kelp species found in the northern Atlantic, is presented. Lipids were extracted and fractionated into neutral lipids, free fatty acids and polar lipids, then derivatized to fatty acid methyl esters prior to GC-MS analysis. A total of 42 fatty acids were identified and quantified, including the n-3 fatty acids α-linolenic acid, stearidonic acid and eicosapentaenoic acid. An n-6/n-3 ratio of 0.8:1 was found in blade and 3.5:1 in stipe, respectively. The ratios vary between the lipid fractions within stipe and blade, with the lowest ratio in the polar lipid fraction of blade. The fatty acid amounts are higher in blade than in stipe, and the highest amounts of n-3 fatty acids are found within the neutral lipid fractions. The amounts of polyunsaturated fatty acids are 3.4 times higher in blade than stipe. This study highlights the compositional differences between the lipid fractions of stipe and blade from L. hyperborea. The amount of polyunsaturated fatty acids, compared to saturated- and monounsaturated fatty acids, as well as the n-6/n-3-ratio, is known to influence human health. In the pharmaceutical, food, and feed industries this can be of importance for production and sale of different health products. Additionally, lipids are today among the unused by products of alginate production, exploiting this material for commercial interest should give both economical and environmental benefits.

Changes in Fatty Acid Composition of two Muscles from Three Different Iberian × Duroc Genotypes After Refrigerated Storage

2008 ◽  
Vol 14 (2) ◽  
pp. 127-137 ◽  
Author(s):  
M.R. Ramírez ◽  
R. Cava
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Lipid Fraction ◽  
Meat Products ◽  
Biceps Femoris ◽  
Longissimus Dorsi ◽  
Meat Production ◽  
Refrigerated Storage ◽  
Chain Pufa ◽  
Lipid Fractions

The changes of the fatty acid (FA) profile of 2 muscles Longissimus dorsi and Biceps femoris from 3 Iberian × Duroc genotypes were studied: GEN1: ♂ Iberian × ♀ Duroc1, GEN2: ♂ Duroc1 × ♀ Iberian; GEN3: ♂ Duroc2 × ♀ Iberian. GEN1 and GEN2 are reciprocal crosses while the difference between GEN2 and GEN3 is the Duroc sire line. The genotype Duroc1 was selected for the production of dry-cured meat products while the genotype Duroc2 was selected for meat production. Longissimus dorsi and Biceps femoris BF from the reciprocal cross showed similar changes in FAs profile after refrigerated storage. However, the Duroc sire line affected the FA profiles of intramuscular fat (IMF) and lipid fractions since some differences were found between GEN2 and GEN3. Meat from GEN3 had the highest level of polyunsaturated fatty acids (PUFA) in IMF and lipid fractions as well as the lowest rate of plasmalogens in polar lipid fraction. After storage, meat from GEN3 showed an increase of long chain PUFA in free fatty acids fraction and the highest increase in the ratio DMA/FA [(dimethylacetals/FAs) × 100] after the refrigerated storage, which was indicative of a higher deterioration of this genotype. Therefore, the crossbreeding of Iberian pigs with Duroc selected genotypes (Duroc2) could affect the changes in the FAs profile of meat under refrigerated storage.

Lipid composition of developing Xenopus laevis embryos

1976 ◽  
Vol 54 (6) ◽  
pp. 578-582 ◽  
Author(s):  
Mary Mes-Hartree ◽  
John B. Armstrong
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Xenopus Laevis ◽  
Lipid Content ◽  
Lipid Composition ◽  
De Novo ◽  
Total Lipid Content ◽  
De Novo Synthesis ◽  
Xenopus Laevis Embryos

The total lipid content, amount of phospholipid, proportions of major polar and neutral lipid classes, and the overall fatty acid composition were examined in Xenopus laevis embryos. No obvious differences were observed in any of the parameters between fertilization and hatching, or between eggs produced by different females. The average lipid content per egg was 113 μg, 31.6 μg of which was phospholipid. The major phospholipids were phosphatidylcholine and sphingomyelin. The major fatty acids were palmitic and oleic acids, but polyunsaturated fatty acids were also present in substantial amounts. The results suggest that significant de novo synthesis of lipids does not occur until after hatching.

scholarly journals A Study on Nutritional Profile of Liver from Captured and Cultured Rohu, Labeo rohita (Cypriniformes: Cyprinidae)

2021 ◽  
pp. 398-408
Author(s):  
Onkar Singh Brraich ◽  
Navpreet Kaur ◽  
Swarndeep Singh Hundal
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Total Lipid ◽  
Lipid Content ◽  
Labeo Rohita ◽  
Total Lipid Content ◽  
Processing Waste ◽  
Cultured Fish

Fish production and fish processing waste have straight connection.  In India, the waste produced during the processing of fish is predicted to be approximate 3.6 million metric tonnes, 48 per cent of the total body weight of Indian and exotic major carps is thrown away as waste (non-edible ). The present research, it was conducted to compare the total lipid content (TLC) and fatty acid composition from the liver of captured and cultured fish, Labeo rohita  (Hamilton) having weight more than 500 gram during different months as well as to evaluate its nutritional quality. Maximum total lipid content (33.33±0.14%) was found in the liver of cultured fish in May month, while the minimum (15.26±0.24%) was in the liver of captured fish in the month of January. During the study, total lipid content was found to be considerably elevated in cultured than captured Fish, Labeo rohita  (Hamilton). The amount of three major groups of fatty acids namely polyunsaturated fatty acids, monounsaturated fatty acids and saturated fatty acids was found to be maximum in captured fish during the month of March 97.19±0.96%, 61.30±0.56% and 95.39±0.31% month of April respectively. Total n-6 polyunsaturated fatty acids were observed to be highest (21.95±8.05%) in the waste of liver in cultured fish during the month of March. Hence, it is concluded that the processing waste (liver) of the captured and cultured, Labeo rohita (Hamilton) is a prosperous resource of the essential fatty acids i.e.  PUFAs and total lipids. Further, it is observed that captured species are rich in fatty acid composition as compared to cultured species. Food industries can manufacture by-products from these high nutritional value contents of   waste for human utilization. EPA and DHA also reduce the risk of various life threatening diseases.

scholarly journals Fatty Acid Composition, Phospholipid Molecules, and Bioactivities of Lipids of the Mud Crab Scylla paramamosain

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Thi Phuong Lan Nguyen ◽  
Van Tuyen Anh Nguyen ◽  
Thi Thanh Trung Do ◽  
Trung Nguyen Quang ◽  
Quoc Long Pham ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Total Lipid ◽  
Lipid Content ◽  
High Resolution Mass Spectrometry ◽  
Total Lipid Content ◽  
Molecular Forms ◽  
Scylla Paramamosain ◽  
Mud Crab ◽  
Anti Inflammatory

The mud crab increases the yield of farming in Southeast Asian countries. Scylla paramamosain, one of four mud crab species belonging to the Scylla genus, is a rich nutrient source during its soft-shell moulting period. In this study, we analysed the total lipid content, fatty acid components, and phospholipid molecular species of the S. paramamosain mud crab. The total lipid content was 1.62 ± 0.08%, which is similar to that of S. serrata previously reported. Twenty-one fatty acids were identified in S. paramamosain. The composition and molecular forms of the phospholipids were identified using high-performance liquid chromatography-high-resolution mass spectrometry. Fifty-four different molecules belonging to six types of phospholipids were identified. Notably, phospholipids were made of fatty acids with C16 : 0; C18 : 0; C20 : 4; C20 : 5; and C22 : 6 main components. The anti-inflammatory and cytotoxic effects of crab lipids and phospholipids were investigated for the first time. The anti-inflammatory activity of the total and polar lipids had IC50 values of 71.5 and 68.6 μg/mL, respectively. The crab polar lipid fraction, which contained phospholipids, also presented high cytotoxic activity toward five cancer cell lines with IC50 values ranging from 85.4 to 95.8 μg/mL.

The effect of long term storage on the lipid reserves and fatty acid composition of cysts and hatched juveniles of Globodera rostochiensis and G. pallida

1998 ◽  
Vol 72 (2) ◽  
pp. 133-141 ◽  
Author(s):  
R.A. Holz ◽  
D.J. Wright ◽  
R.N. Perry
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Neutral Lipid ◽  
Lipid Content ◽  
Neutral Lipids ◽  
Lipid Fraction ◽  
Neutral Lipid Fraction ◽  
Fatty Acid Profiles ◽  
Acidic Phospholipids

AbstractThe lipid composition of three batches of single generation cysts of Globodera rostochiensis, stored dry at 4°C for 1,7 and 13 years, comprised 81%, 74% and 53% neutral lipids, 14%, 18% and 27% non-acidic phospholipids and 5%, 8% and 20% free fatty acids, respectively. Lipids in eggs from two batches of G. pallida cysts, stored for 3 and 7 years, comprised 80% and 67% neutral lipids, 15% and 23% non-acidic phospholipids and 5% and 10% free fatty acids, respectively. All batches contained the same fatty acids which were dominated by C18:l, C20:l and C20:4. The fatty acid profiles of hatched J2 of G. rostochiensis from two batches, stored for 1 and 9 years, differed only in their free fatty acid fractions. Thus, while it is not possible to determine the age of cysts by their fatty acid profile, it may be possible to use the relative amounts of the main lipid classes as an indicator of age. Four batches of hatched J2 of G. pallida were investigated, with sample A hatched during the second week in potato root diffusate, B during week 3, C during week 4 and D during weeks 5 and 6 and stored for 3.5 days (on average) after hatching. Total lipid content was 27.2%, 31.5%, 18.5% and 6.3% of the dry weight for A, B, C and D, respectively. In the neutral lipid fraction of D an increase in C18:l and to a lesser extent C18:2 was observed. In the free fatty acid fraction of sample D, the percentages of C18:l, C18:2 and C18:3 were greater but the percentages of C20:3 and C20:4 were smaller compared with sample C. Fresh early hatched J2 of G. rostochiensis were compared with later hatched and stored (for 13 days on average) individuals for their lipid content and fatty acid composition. The lipid content was 26.1% and 11.4% in fresh and stored J2, respectively. Total lipid consisted of 77% and 70% neutral lipid, 18% and 26% non-acidic phospholipid and 6% and 4% free fatty acid in fresh and stored J2, respectively. In the neutral lipid fraction of stored J2 C18:l, C16:0 and C18:0 increased, whereas C20:4, C20:l and C20:3 decreased. Therefore, both neutral lipid and free fatty acid fractions showed changes in their fatty acid profiles after long delayed hatching and/or storage in both PCN species.

scholarly journals Effects of oxygen tension on the lipid composition of Azotobacter chroococcum

1979 ◽  
Vol 25 (11) ◽  
pp. 1239-1244 ◽  
Author(s):  
Edward B. Reczek ◽  
David N. Burton
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Total Lipid ◽  
Lipid Content ◽  
Octadecenoic Acid ◽  
Total Lipid Content ◽  
Azotobacter Chroococcum ◽  
Neutral Lipid Content ◽  
Intense Aeration

Lipid content and composition were determined in Azotobacter chroococcum grown, under nitrogen-fixing conditions, in continuous culture with intense aeration under atmospheres containing between 5 and 40% O2. Total lipid content remained almost constant at approximately 9% of dry weight.Phospholipid content was maximal at 20% O2 where it accounted for 92% of total lipid, and was minimal at 40% O2. Phosphatidylethanolamine was the only species of phosphatide detected in this fraction. Neutral lipid content was minimal at 20% O2 and maximal at 40% O2 where it represented approximately 30% of the total lipid. Glycolipid remained between 2 and 13% of total lipid throughout. The principal fatty acids of A. chroococcum were hexadecanoic (C16:0), hexadecenoic (C16:1), and octadecenoic acid (C18:1) at all O2 tensions, but C18:1 increased at the expense of C16:1 at higher O2 tensions, particularly in free fatty acid and phospholipid fractions. [U-14C]acetate was readily incorporated into lipid at both 20 and 40% O2 but total incorporation was much greater at 20% O2.

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