Prevalence of specific immunoglobulin E and G against Aspergillus fumigatus in patients with asthma

Background and Purpose: Aspergillus fumigatus as a ubiquitous fungus can be found in the respiratory tract of the asthmatic and healthy people. The inhalation of Aspergillus spores leads to an immune response in individuals with asthma and results in the aggravation of the clinical symptoms. The present study aimed to investigate the prevalence of specific immunoglobulin E and G (IgE and IgG) against A.fumigatus in asthmatic patients.Materials and Methods: This study was conducted on 200 consecutive patients with moderate to severe asthma referring to Masih Daneshvari hospital Tehran, Iran, from January 2016 to February 2018. Skin prick test (SPT) was performed in all subjects with Aspergillus allergens. Moreover, all patients underwent specific IgE testing for Aspergillus using Hycor method. Enzyme immune assay was applied to measure total IgE and Aspergillus-specific IgG.Results: According to the results, the mean age of the patients was 45.8 years (age range: 18-78 years). The mean levels of total IgE and Aspergillus specific IgE in asthmatic patients were obtained as 316.3 (range: 6-1300 IU/ml) and 1.5 (range: 0.1-61.3 IU/ml), respectively. Out of 200 patients, 27 (13.5%), 65 (32.5%), 22 (11.0%), and 86 (43.0%) cases had positive Aspergillus SPT, total IgE of > 417 IU/ml, Aspergillus-specific IgE, and IgG, respectively. The level of these variables in patients with severe asthma were 16 (16.5%), 36 (37.1%), 15 (15.5%), and 46 (47.4%), respectively.Conclusion: As the findings indicated, reactivity to Aspergillus is a remarkable phenomenon in asthmatic patients. It is also emphasised that the climatic condition may affect the positive rate of hypersensitivity to Aspergillus.

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Evaluation of a fully automated and completely quantitative allergen-specific IgE and total IgE detection assay

Analytical Methods ◽

10.1039/d1ay01172c ◽

2021 ◽

Author(s):

Huimin Huang ◽

Zhifeng Huang ◽

Peiyan Zheng ◽

Nili Wei ◽

Xueqing Liang ◽

...

Keyword(s):

Immunoglobulin E ◽

Detection System ◽

Specific Ige ◽

Quantitative Detection ◽

Total Ige ◽

Detection Assay ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin

Based on the current urgent need for an in vitro quantitative detection system for allergens in most hospitals in China, we introduced a novel allergen-specific immunoglobulin E detection system that...

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Total and Specific Immunoglobulin E and Their Relationship to Respiratory Symptoms in Quebec Children and Adolescents

Canadian Respiratory Journal ◽

10.1155/2005/248714 ◽

2005 ◽

Vol 12 (8) ◽

pp. 426-432 ◽

Cited By ~ 6

Author(s):

Benoît Levesque ◽

Jean-François Duchesne ◽

Suzanne Gingras ◽

Pierre Allard ◽

Edgar Delvin ◽

...

Keyword(s):

Children And Adolescents ◽

Respiratory Symptoms ◽

Immunoglobulin E ◽

Geometric Mean ◽

Allergic Sensitization ◽

Population Sample ◽

Ethnic Origin ◽

Total Ige ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin

BACKGROUND: Respiratory disease is a major cause of morbidity in young people. It is now recognized that atopy plays an important role in the development of chronic respiratory symptoms in children.OBJECTIVE: To examine the determinants and consequences of serum total and specific immunoglobulin E (IgE) in a general population sample of Québec children and adolescents.METHODS: In 1999, 2349 children and adolescents (nine, 13 or 16 years of age) who had participated in a respiratory symptom and disease questionnaire had their total IgE measured. Of these participants, a subsample of 451 children and adolescents was analyzed to detect antibodies to eight specific allergens (ie, allergens of dust mites [Dermatophagoides farinae and Dermatophagoides pteronyssinus], cat, dog, ragweed, Timothy grass, mould [alternaria] and cockroach).RESULTS: The geometric mean of the total IgE was 44.4 U/mL among all participants. Concentrations were higher in boys and increased with age. More than 41% of the participants were sensitized to at least one specific allergen. Such sensitization was strongly associated with the occurrence of respiratory conditions and symptoms, namely asthma, wheezing and rhinitis. Family history, school location and ethnic origin had an impact on the prevalence of atopy and total IgE levels.CONCLUSIONS: Allergic sensitization is a major determinant in the development of asthma, wheezing and rhinitis in children and adolescents in the province of Québec.

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Enterotoxin-Specific Immunoglobulin E Responses in Humans after Infection or Vaccination with Diarrhea-Causing Enteropathogens

Infection and Immunity ◽

10.1128/iai.68.10.6077-6081.2000 ◽

2000 ◽

Vol 68 (10) ◽

pp. 6077-6081 ◽

Cited By ~ 9

Author(s):

Firdausi Qadri ◽

Muhammad Asaduzzaman ◽

Christine Wennerås ◽

Golam Mohi ◽

M. John Albert ◽

...

Keyword(s):

North American ◽

Immunoglobulin E ◽

Specific Ige ◽

Cholera Vaccine ◽

Total Ige ◽

B Subunit ◽

The North ◽

Specific Immunoglobulin E ◽

Oral Cholera Vaccine ◽

Ige Response

ABSTRACT Cholera toxin (CT)-specific antibody responses of the immunoglobulin E (IgE) isotype in the sera of adult patients suffering from infection with either Vibrio cholerae O1, V. cholerae O139, or enterotoxigenic Escherichia coli(ETEC) were analyzed and compared with those in the sera of volunteers immunized with a bivalent B subunit O1/O139 whole-cell cholera vaccine. A significant IgE response to CT was observed in 90% of the patients with V. cholerae O1 infection (18 of 20; P = <0.001) and 95% of the patients with V. cholerae O139 infection (19 of 20; P = <0.001). Similarly, the majority of the patients with ETEC diarrhea (83%; 13 of 15) showed a positive IgE response to CT. Eight of 10 North American volunteers (80%) orally challenged with V. cholerae O1 showed CT-specific IgE responses (P = 0.004). In contrast, Swedish volunteers immunized with the oral cholera vaccine showed no IgE responses to CT (P value not significant). During the study period, total IgE levels in the sera of the diarrheal patients, the North American volunteers, and the Swedish cholera vaccinees alike remained unchanged. However, the total IgE levels in the sera of patients and healthy Bangladeshi controls were on average 89-fold higher than those in the sera of the healthy Swedish volunteers and 34-fold higher than those in the sera of the North American volunteers.

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Interpretation of food specific immunoglobulin E levels in the context of total IgE

Annals of Allergy Asthma & Immunology ◽

10.1016/j.anai.2013.05.012 ◽

2013 ◽

Vol 111 (1) ◽

pp. 20-24 ◽

Cited By ~ 11

Author(s):

Tara J. Federly ◽

Bridgette L. Jones ◽

Hongying Dai ◽

Chitra Dinakar

Keyword(s):

Immunoglobulin E ◽

Total Ige ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin

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Detection of Cross-Reactivity for Atopic Immunoglobulin E against Multiple Allergens

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.10.2.229-232.2003 ◽

2003 ◽

Vol 10 (2) ◽

pp. 229-232 ◽

Cited By ~ 4

Author(s):

Yee-Hsuan Chiou ◽

Chung-Yee Yuo ◽

Lin-Yu Wang ◽

Shiao-ping Huang

Keyword(s):

Immunoglobulin E ◽

Competitive Inhibition ◽

Allergic Patient ◽

Specific Ige ◽

Cross Reactivity ◽

Allergen Avoidance ◽

Ige Binding ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin ◽

The Common

ABSTRACT The existence of specific immunoglobulin E (IgE) allows us to determine the allergens that cause the allergic disease. For the purposes of allergen avoidance and immunotherapy, the measurement of specific IgE is widely applied in clinical laboratories. However, if IgE from the serum of an allergic patient exhibits reactivity to multiple allergens, it would cause a problem. The present study analyzes whether the serum IgE with multiple reactivity is due to the presence of unique IgE against the common epitope shared by different allergens or the presence of multiple IgEs against different epitopes on different allergens. The quantitative-competitive inhibition tests and the immunoblotting were applied to analyze the immunosimilarity among examined allergens. The result shows that the competitive inhibition of IgE binding between shrimp and crab allergens is higher than those between either shrimp and cockroach or between crab and cockroach. Furthermore, the results of immunoblotting are consistent with those of quantitative-competitive inhibition tests. These results allow us to detect the cross-reactivity for atopic IgE against multiple allergens.

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Local production of aspergillus fumigatus specific Immunoglobulin E in nasal polyps

The Laryngoscope ◽

10.1097/00005537-200310000-00026 ◽

2010 ◽

Vol 113 (10) ◽

pp. 1798-1802 ◽

Cited By ~ 13

Author(s):

M. Weschta ◽

D. Rimek ◽

M. Formanek ◽

D. Polzehl ◽

H. Riechelmann

Keyword(s):

Aspergillus Fumigatus ◽

Nasal Polyps ◽

Immunoglobulin E ◽

Local Production ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin

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Measurement of specific immunoglobulin E: intermethod comparison and standardization

Clinical Chemistry ◽

10.1093/clinchem/44.9.1974 ◽

1998 ◽

Vol 44 (9) ◽

pp. 1974-1979 ◽

Cited By ~ 31

Author(s):

Mario Plebani ◽

Daniela Bernardi ◽

Daniela Basso ◽

Franco Borghesan ◽

Diego Faggian

Keyword(s):

Roc Analysis ◽

Second Generation ◽

Immunoglobulin E ◽

Specific Ige ◽

Clinical Sensitivity ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin ◽

Significant Disagreement ◽

Quantitative Results ◽

New System

Abstract Recently introduced “second-generation” techniques for specific IgE measurement have produced some analytical improvement, offering better clinical sensitivity than previous techniques. The aims of our study were to compare the analytical and clinical performances of four second-generation techniques for allergen-specific IgE measurement in serum and to ascertain whether the new system for reporting quantitative results contributes to greater clinical agreement between findings using the techniques considered. Allergen-specific IgE was measured using the CAP® System, CARLA®, ENEA®, and AlaSTAT®, and the findings were compared. A significant disagreement was found between CAP and ENEA for all allergens and between CAP and CARLA for D1 and G5. However, the clinical discrepancies were reduced by selecting method-specific thresholds using ROC analysis. Second-generation techniques enable us to obtain better standardization of results; however, the identification of a specific threshold appears to be a prerequisite for the appropriate clinical interpretation of the test findings.

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Diagnosis and Management of Severe Asthma in Switzerland: Analysis of Survey Results Conducted with Specialists and General Practitioners

Respiration ◽

10.1159/000514628 ◽

2021 ◽

pp. 1-12

Author(s):

Peter Jandus ◽

Mélanie Duc ◽

Bianca Christine Fay

Keyword(s):

General Practitioners ◽

Severe Asthma ◽

Immunoglobulin E ◽

Therapeutic Strategies ◽

Prescribing Patterns ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin ◽

Background Therapy ◽

Oral Corticosteroids ◽

Survey Results

Background: Severe asthma commonly affects 5–10% of the asthmatic population and accounts for approximately 50% of the overall asthma costs. Objective: This analysis investigated how severe asthma is diagnosed, treated, and managed by specialists and general practitioners (GPs) in Switzerland. Methods: Two surveys, one each among specialists (N = 44) and GPs (N = 153), were conducted to understand their self-perception on diagnosis, treatment, and management of severe asthma. Results: Fifty-five percent of the specialists felt very confident and 43% confident in recognizing the symptoms of severe asthma and diagnosing severe asthma. In contrast, 9% of the GPs were very confident and 59% confident in diagnosing severe asthma. More specific diagnostic tests for severe asthma, like total and specific immunoglobulin E levels and measurement of the fraction of exhaled nitric oxide, were run by specialists (χ2 = 171.4; df = 15, p < 0.001). GPs and specialists were using different measurements to assess severe asthma (χ2 = 385.2; df = 13, p < 0.001) and their prescribing patterns differed significantly (χ2 = 189.8; df = 10, p < 0.001). GPs referred patients with severe asthma if the diagnosis was unclear (24%), if treatment failure occurred (26%), and if the patients were at high risk (41%). Conclusions: Oral corticosteroids (OCSs) are considered as background therapy for severe asthma by GPs and specialists. In order to reduce the OCS burden, there is a need to improve the awareness for other add-on therapies. A joint collaboration between GPs and specialists is the key to leverage therapeutic strategies together.

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Dysregulated specific IgE production to bystander foods in children with peanut allergy but not egg allergy

Journal of Food Allergy ◽

10.2500/jfa.2021.3.210005 ◽

2021 ◽

Vol 3 (1) ◽

pp. 24-31 ◽

Cited By ~ 1

Author(s):

Nurcicek Padem ◽

Kristin Erickson ◽

Meagan Yong ◽

Melanie Makhija ◽

Kathryn E. Hulse ◽

...

Keyword(s):

Food Allergy ◽

Peanut Allergy ◽

Immunoglobulin E ◽

Specific Ige ◽

Egg White ◽

Total Ige ◽

Control Subjects ◽

Specific Immunoglobulin E ◽

Natural Tolerance ◽

And Control

Background: Food specific immunoglobulin E (sIgE) levels are associated with the development of allergic responses and are used in the clinical evaluation of food allergy. Food sIgG4 levels have been associated with tolerance or clinical nonresponsiveness, particularly in interventional studies. Objective: We aimed to characterize food-specific antibody responses and compare responses with different foods in food allergy. Methods: Serum sIgA, sIgG4, and sIgE to whole peanut, egg white, and wheat, along with total IgE were measured in 57 children. Children with food allergy, children with natural tolerance, and controls were studied. The Mann-Whitney test or Kruskall Wallis test with the Dunn correction were used for statistical analysis. Results: As expected, total IgE levels were highest in the subjects with food allergy compared with the subjects who were nonallergic (p < 0.001) or the subjects who were naturally tolerant (p < 0.001). Peanut sIgE levels were higher in subjects with peanut allergy compared with the subjects who were naturally tolerant (p < 0.0001) and the control subjects (p < 0.03). Interestingly, peanut sIgG4 levels were also highest in children with peanut allergy compared with subjects who were naturally tolerant and control subjects (p = 0.28 and p < 0.001, respectively). Subjects with peanut allergy alone had comparable egg white sIgE levels to children with egg white allergy. In addition, the subjects with peanut allergy alone also had higher levels of egg white and wheat sIgE compared with the control subjects (p < 0.02 and p = 0.001, respectively). In contrast, the subjects with egg white allergy did not demonstrate elevated peanut or wheat sIgE levels. Conclusion: These novel findings suggested that IgE production is dysregulated in patients with peanut allergy, who are much less likely to outgrow their allergy, and suggest that the mechanisms that drive more persistent forms of food allergy may be distinct from more transient forms of food allergy.

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Local Atopy in Childhood Adenotonsillar Hypertrophy

American Journal of Rhinology and Allergy ◽

10.1177/1945892418765003 ◽

2018 ◽

Vol 32 (3) ◽

pp. 160-166 ◽

Cited By ~ 9

Author(s):

Kyu-Sup Cho ◽

Seong Heon Kim ◽

Sung-Lyong Hong ◽

Jaeyoung Lee ◽

Sue Jean Mun ◽

...

Keyword(s):

Allergic Rhinitis ◽

Risk Factor ◽

Immunoglobulin E ◽

Clinical Symptoms ◽

Allergic Inflammation ◽

Inhalant Allergen ◽

Adenotonsillar Hypertrophy ◽

Nasal Symptoms ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin

Background Although the cause of adenotonsillar hypertrophy remains unknown, some studies have shown that allergy may be a risk factor. Purpose This study determined the levels of allergen-specific immunoglobulin E (sIgE) in the adenotonsillar tissues of children with adenotonsillar hypertrophy and evaluated the clinical significance of local atopy in adenotonsillar tissues. Methods We measured 21 types of specific immunoglobulin E in the serum and adenotonsillar tissues of 102 children with adenotonsillar hypertrophy and compared the sensitization patterns of the serum and local tissues. The patients were divided into three groups—atopy, local atopy, and nonatopy—according to the sensitization of serum and adenotonsillar tissues, and the clinical symptoms among the groups were analyzed. Results Seventy-two (70.6%) children with adenotonsillar hypertrophy were sensitized to more than one allergen in the serum and/or adenotonsillar tissue. Thirty (29.4%) children had no IgE positivity to any allergen in both serum and adenotonsillar tissues. Fifty-five (53.9%) were sensitized to at least one allergen in the serum. Seventy (68.6%) were sensitized to at least one allergen in the adenotonsillar tissue. Seventeen (36.2%) of 47 children with specific immunoglobulin E-negative serum had specific immunoglobulin E-positive adenotonsillar tissues. The rate of specific immunoglobulin E was significantly higher in local tissues than in serum. The rate of inhalant allergen specific immunoglobulin E was significantly higher in the adenoids than in the tonsils. However, the rate of food allergen specific immunoglobulin E was significantly higher in the tonsils than adenoids. The lifetime prevalence of asthma and allergic rhinitis, recent symptoms or treatment of allergic rhinitis, and severity of nasal symptoms (rhinorrhea, sneezing, and nasal itching) were significantly higher in children with local atopy than with nonatopy. Conclusions These results confirm that allergic response may be a risk factor for adenotonsillar hypertrophy. Local allergic inflammation may play an important role in childhood adenotonsillar hypertrophy, and local atopy in adenotonsillar tissues can cause respiratory allergic symptoms in children.

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