scholarly journals Detection of Cross-Reactivity for Atopic Immunoglobulin E against Multiple Allergens

2003 ◽  
Vol 10 (2) ◽  
pp. 229-232 ◽  
Author(s):  
Yee-Hsuan Chiou ◽  
Chung-Yee Yuo ◽  
Lin-Yu Wang ◽  
Shiao-ping Huang
Keyword(s):  
Immunoglobulin E ◽  
Competitive Inhibition ◽  
Allergic Patient ◽  
Specific Ige ◽  
Cross Reactivity ◽  
Allergen Avoidance ◽  
Ige Binding ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin ◽  
The Common

ABSTRACT The existence of specific immunoglobulin E (IgE) allows us to determine the allergens that cause the allergic disease. For the purposes of allergen avoidance and immunotherapy, the measurement of specific IgE is widely applied in clinical laboratories. However, if IgE from the serum of an allergic patient exhibits reactivity to multiple allergens, it would cause a problem. The present study analyzes whether the serum IgE with multiple reactivity is due to the presence of unique IgE against the common epitope shared by different allergens or the presence of multiple IgEs against different epitopes on different allergens. The quantitative-competitive inhibition tests and the immunoblotting were applied to analyze the immunosimilarity among examined allergens. The result shows that the competitive inhibition of IgE binding between shrimp and crab allergens is higher than those between either shrimp and cockroach or between crab and cockroach. Furthermore, the results of immunoblotting are consistent with those of quantitative-competitive inhibition tests. These results allow us to detect the cross-reactivity for atopic IgE against multiple allergens.

scholarly journals Prevalence of specific immunoglobulin E and G against Aspergillus fumigatus in patients with asthma

2019 ◽  
Author(s):  
Newsha Hedayati ◽  
Vida Mortezaee ◽  
Seyed Alireza Mahdaviani ◽  
Maryam Sadat Mirenayat ◽  
Maryam Hassanzad ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Severe Asthma ◽  
Immunoglobulin E ◽  
Clinical Symptoms ◽  
Specific Ige ◽  
Total Ige ◽  
Asthmatic Patients ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin ◽  
The Mean

Background and Purpose: Aspergillus fumigatus as a ubiquitous fungus can be found in the respiratory tract of the asthmatic and healthy people. The inhalation of Aspergillus spores leads to an immune response in individuals with asthma and results in the aggravation of the clinical symptoms. The present study aimed to investigate the prevalence of specific immunoglobulin E and G (IgE and IgG) against A.fumigatus in asthmatic patients.Materials and Methods: This study was conducted on 200 consecutive patients with moderate to severe asthma referring to Masih Daneshvari hospital Tehran, Iran, from January 2016 to February 2018. Skin prick test (SPT) was performed in all subjects with Aspergillus allergens. Moreover, all patients underwent specific IgE testing for Aspergillus using Hycor method. Enzyme immune assay was applied to measure total IgE and Aspergillus-specific IgG.Results: According to the results, the mean age of the patients was 45.8 years (age range: 18-78 years). The mean levels of total IgE and Aspergillus specific IgE in asthmatic patients were obtained as 316.3 (range: 6-1300 IU/ml) and 1.5 (range: 0.1-61.3 IU/ml), respectively. Out of 200 patients, 27 (13.5%), 65 (32.5%), 22 (11.0%), and 86 (43.0%) cases had positive Aspergillus SPT, total IgE of > 417 IU/ml, Aspergillus-specific IgE, and IgG, respectively. The level of these variables in patients with severe asthma were 16 (16.5%), 36 (37.1%), 15 (15.5%), and 46 (47.4%), respectively.Conclusion: As the findings indicated, reactivity to Aspergillus is a remarkable phenomenon in asthmatic patients. It is also emphasised that the climatic condition may affect the positive rate of hypersensitivity to Aspergillus.

scholarly journals VALIDA project: Validation of allergy in vitro diagnostics assays (Tools and recommendations for the assessment of in vitro tests in the diagnosis of allergy)

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
María L. Casas ◽  
Ángel Esteban ◽  
Miguel González-Muñoz ◽  
Moisés Labrador-Horrillo ◽  
Mariona Pascal ◽  
...  
Keyword(s):  
Immunoglobulin E ◽  
Allergic Patient ◽  
Specific Ige ◽  
In Vitro Tests ◽  
Consensus Document ◽  
Specific Immunoglobulin E ◽  
The Impact

AbstractIn vitro allergen-specific immunoglobulin E (IgE) detection and quantification tests are routinely performed in clinical laboratories to diagnose patients with a suspected allergy. Numerous commercial assays are available to test for allergies, but the results can vary widely, thereby influencing both diagnosis and treatment. Given the challenges posed by differences in the various assays for in vitro determination of specific IgE, a group of experts has compiled in a document a series of recommendations on the implications that the use of a certain in vitro technique may have and the impact on the management of the allergic patient that the differences between the various techniques represent. The reading and analysis of this consensus document will help to understand the implications of the change of in vitro diagnostic method in the management of the patient with allergy, in the quality of life and in the socioeconomic costs associated with the disease.

Evaluation of a fully automated and completely quantitative allergen-specific IgE and total IgE detection assay

2021 ◽  
Author(s):  
Huimin Huang ◽  
Zhifeng Huang ◽  
Peiyan Zheng ◽  
Nili Wei ◽  
Xueqing Liang ◽  
...  
Keyword(s):  
Immunoglobulin E ◽  
Detection System ◽  
Specific Ige ◽  
Quantitative Detection ◽  
Total Ige ◽  
Detection Assay ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin

Based on the current urgent need for an in vitro quantitative detection system for allergens in most hospitals in China, we introduced a novel allergen-specific immunoglobulin E detection system that...

scholarly journals Measurement of specific immunoglobulin E: intermethod comparison and standardization

1998 ◽  
Vol 44 (9) ◽  
pp. 1974-1979 ◽  
Author(s):  
Mario Plebani ◽  
Daniela Bernardi ◽  
Daniela Basso ◽  
Franco Borghesan ◽  
Diego Faggian
Keyword(s):  
Roc Analysis ◽  
Second Generation ◽  
Immunoglobulin E ◽  
Specific Ige ◽  
Clinical Sensitivity ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin ◽  
Significant Disagreement ◽  
Quantitative Results ◽  
New System

Abstract Recently introduced “second-generation” techniques for specific IgE measurement have produced some analytical improvement, offering better clinical sensitivity than previous techniques. The aims of our study were to compare the analytical and clinical performances of four second-generation techniques for allergen-specific IgE measurement in serum and to ascertain whether the new system for reporting quantitative results contributes to greater clinical agreement between findings using the techniques considered. Allergen-specific IgE was measured using the CAP® System, CARLA®, ENEA®, and AlaSTAT®, and the findings were compared. A significant disagreement was found between CAP and ENEA for all allergens and between CAP and CARLA for D1 and G5. However, the clinical discrepancies were reduced by selecting method-specific thresholds using ROC analysis. Second-generation techniques enable us to obtain better standardization of results; however, the identification of a specific threshold appears to be a prerequisite for the appropriate clinical interpretation of the test findings.

scholarly journals Cross-reactivity of each fraction among cereals in children with wheat allergy

2021 ◽  
Author(s):  
Mari Takei ◽  
Akemi Saito ◽  
Noriyuki Yanagida ◽  
Sakura Sato ◽  
Motohiro Ebisawa
Keyword(s):  
Immunoglobulin E ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Wheat Allergy ◽  
Protein Levels ◽  
Inhibition Elisa ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin ◽  
Lower Protein

Background: Cross-reactivity between wheat and other cereals is an essential issue in the management of wheat allergy. Few studies have reported in vitro cross-reactivity in immediate-type wheat allergy. This study aimed to examine cross-reactivity of the three fractions (albumin/globulin, gliadin, and glutenin fractions) among cereals in children with wheat allergy. Methods: Sera from 128 children with immediate-type wheat allergy were collected. We measured specific immunoglobulin E (sIgE) levels against each fraction of wheat, barley, and rye by using an enzyme-linked immunosorbent assay (ELISA). Cross-reactivities of each fraction among wheat, barley, and rye were examined via inhibition ELISA. Results: All subjects were sensitized to all the fractions of wheat, and also those of barley and rye. The wheat sIgE levels were significantly higher than those of barley and rye in all the fractions (p ≤ 0.001) and were significantly correlated with sIgE levels to them in each fraction (r = 0.887–0.969, p < 0.001). On inhibition ELISA, wheat inhibited the IgE binding to most of the solid phases at the lower protein levels compared to barley and rye in all fractions. Conclusions: In children with immediate-type wheat allergy, sensitization to all the three fractions of wheat was observed. In addition, they showed sensitization to barley and rye caused by in vitro cross-reactivity with wheat in each fraction. When managing children with wheat allergy, sensitization to barley and rye caused by the cross-reactivities should be considered.

DETERMINATION OF THE LEVEL OF SPECIFIC IMMUNOGLOBULIN E IN THE BLOOD OF CATS WITH OB-STRUCTIVE BRONCHITIS WITH AN ALLER GENIC COMPONENT

2020 ◽  
Vol 3 ◽  
pp. 154-157
Author(s):  
L.I. Sabirzyanova ◽  
◽  
V.V. Kryukova ◽  
Keyword(s):  
Immunoglobulin E ◽  
Chenopodium Album ◽  
Specific Ige ◽  
House Dust Mites ◽  
Intradermal Testing ◽  
Acarus Siro ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin ◽  
Reac Tions ◽  
High Level

The article presents the results of blood testing of cats with obstructive bronchitis for specific immunoglobulin E in order to confirm the allergic etiology of the disease. The study involved 16 animals, of which an increased level of specific IgE was detected in 12 animals, 1 cat had uncertaine result, and only 3 animals showed a level within the physiological norm. Comparing the ob-tained results with the data of allergic skin intradermal testing, it can be seen that the largest number of registered allergic reac-tions is associated with a high level of spe-cific immunoglobulin E in the blood serum of animals, immunized by antigens, coming from the environment. That is, the antigens of house dust mites, especially mites of the genus Dermatophagoides farinae and Dermatophagoides Pteronyssinus. Thus, the proportion of animals that had an allergic reaction to these ticks was 56.3 %. We ob-served slightly lower allergenicity for ticks of the genus Tugorhadis putrescentiae (37.5 %), Acarus siro (37.5%). Less than 7 % of allergic reactions were observed to antigens of herbs, in particular, meadow thymothy (phelum pretense), white Marion (Chenopodium album L.) and plantain ma-jor (Plantago major). There was no immu-nological reaction to plant components: hazel (Corylus), common wormwood (Artemisia vulgaris). The results obtained using enzyme immunoassay (ELISA) were highly common with the results obtained during intra-dermal allergen testing. We can conclude that determining the level of IgE immunoglobulin in the blood of cats with signs of OB is an objective diagnostic criterion for confirming the allergic nature of obstructive bronchitis in cats.

scholarly journals Chemotherapy for Schistosomiasis in Ugandan Fishermen: Treatment Can Cause a Rapid Increase in Interleukin-5 Levels in Plasma but Decreased Levels of Eosinophilia and Worm-Specific Immunoglobulin E

2004 ◽  
Vol 72 (7) ◽  
pp. 4023-4030 ◽  
Author(s):  
Colin M. Fitzsimmons ◽  
Sarah Joseph ◽  
Frances M. Jones ◽  
Claus M. Reimert ◽  
Karl F. Hoffmann ◽  
...  
Keyword(s):  
Transforming Growth Factor ◽  
Immunoglobulin E ◽  
Transforming Growth Factor Β ◽  
Specific Ige ◽  
Blood Cultures ◽  
Interleukin 4 ◽  
Immune Reactions ◽  
Interleukin 5 ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin

ABSTRACT Chemotherapy for blood-dwelling schistosomes kills the worms and exposes parasite antigen to the circulation. In many people from areas of endemicity, this treatment increases parasite-specific immunoglobulin E (IgE) and other Th2 responses in the months following therapy, responses that have been associated with subsequent resistance to reinfection. Here we investigate much earlier changes in immune reactions after praziquantel therapy in Schistosoma mansoni-infected fishermen working in an area of high transmission in Uganda. The subjects gave blood before treatment and at 1 and 21 days posttreatment. Blood cultures were incubated with schistosome soluble worm antigen (SWA) or soluble egg antigen (SEA). Interleukin-4 (IL-4), IL-5, IL-10, IL-13, gamma interferon, and transforming growth factor β levels were measured in the cultures and in plasma. A marked transient increase in plasma IL-5 levels was observed in 75% of the subjects (n = 48) by 1 day posttreatment. This response was dependent on pretreatment intensity of infection and was accompanied by a transient decrease in eosinophil numbers. One day posttreatment, blood cultures from the 16 subjects with the greatest increase in plasma IL-5 level (>100 pg/ml) displayed reduced IL-5, IL-13, and IL-10 responses to SWA, and in contrast to the rest of the cohort, these high-IL-5 subjects displayed reduced levels of SWA-specific IgE in plasma 21 days posttreatment. Twenty months after treatment, the intensity of reinfection was positively correlated with the increase in plasma IL-5 level seen 1 day posttreatment. These studies describe the heterogeneity in early immune reactions to treatment, identifying subgroups who have different patterns of reaction and who may have different capacities to mount the responses that have been associated with resistance to reinfection.

scholarly journals New data about consistency of skin prick-tests results with specific immunoglobulin E level and food allergy symptoms in children of opisthorchiasis endemic areas

2010 ◽  
Vol 9 (5) ◽  
pp. 72-76
Author(s):  
L. M. Ogorodova ◽  
O. S. Fyodorova ◽  
I. A. Deyev
Keyword(s):  
Food Allergy ◽  
Immunoglobulin E ◽  
Specific Ige ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin ◽  
Skin Prick Tests ◽  
Endemic Areas

Aim: to investigate the consistency of skin prick-tests results with specific IgE level and symptoms of food allergy. The criteria of food allergy in children of opisthorchiasis endemic areas were developed: symptoms of food allergy within 2 hours, skin pricktests weal size ≥1 mm and (or) specific IgE ≥0,35 kUA/l.

scholarly journals Allergic diseases attributable to atopy in a population sample of Asian children

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chao-Yi Wu ◽  
Hsin-Yi Huang ◽  
Wen-Chi Pan ◽  
Sui-Ling Liao ◽  
Man-Chin Hua ◽  
...  
Keyword(s):  
Immunoglobulin E ◽  
Population Attributable Risk ◽  
Population Sample ◽  
International Study ◽  
Allergic Diseases ◽  
Population Based ◽  
School Age Children ◽  
Specific Immunoglobulin E ◽  
Asian Children ◽  
Specific Immunoglobulin

AbstractThe proportion of allergic diseases attributable to atopy remains a subject of controversy. This study aimed to estimate the population risk of physician-diagnosed asthma, rhinitis and eczema attributed to atopy among a population sample of Asian school-age children. Asian children aged 5–18 years (n = 1321) in the Prediction of Allergies in Taiwanese CHildren (PATCH) study were tested for serum allergen-specific immunoglobulin E. Physician-diagnosed asthma, rhinitis and eczema were assessed by a modified International Study of Asthma and Allergies in Childhood questionnaire. Atopy was defined as the presence of serum allergen-specific immunoglobulin E. In this population-based study, 50.4% of the subjects with asthma, 46.3% with rhinitis, and 46.7% with eczema were attributable to atopy. The population attributable risk (PAR) of atopy for three allergic diseases was higher in adolescents (asthma, 54.4%; rhinitis, 59.6%; eczema, 49.5%) than younger children aged less than 10 years (asthma, 46.9%; rhinitis, 39.5%; eczema, 41.9%). Among the seven allergen categories, sensitization to mites had the highest PARs for all three allergic diseases (51.3 to 64.1%), followed by sensitization to foods (asthma, 7.1%; rhinitis, 10.4%; eczema 27.7%). In conclusion, approximately half (46.3 to 50.4%) of Asian children in Taiwan with allergic diseases are attributable to atopy.

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