Molecular Detection of Human Cytomegalovirus in Semen of infertile Men in Tehran

2021 ◽  
Author(s):  
Maryam Moradi ◽  
Somayeh Sadat Moochani ◽  
Nazila Yamini ◽  
Davod Javanmard ◽  
Arezoo Marjani ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Human Cytomegalovirus ◽  
Medical Sciences ◽  
Sexually Transmitted ◽  
Infertile Men ◽  
Fragmentation Index ◽  
Significant Difference ◽  
Clear Result ◽  
The Mean ◽  
Dna Fragmentation Index

Background and Aims: Human cytomegalovirus (HCMV) can induce latency and evade the immune system. The latent virus can reactive later in older ages and due to immunosuppressive conditions. Sexually transmitted diseases and viruses can influence the male reproductive system, and members of the Herpesviridae family are one of these important viruses. Regarding the importance of herpesviruses, specially HCMV, this research aimed to evaluate the prevalence of HCMV in semen samples of infertile men. Materials and Methods: Semen samples were collected from infertility centers affiliated to Iran University of Medical Sciences. The chromatin state was assessed by DNA fragmentation index. Nucleic acids were extracted from the semen specimens, and a real-time polymerase chain reaction assay was performed to detect HCMV DNA. Results: Enrolled patients were 82 infertile men. The mean age of participants was 37.3 ± 6.1 years, and the mean motility and DNA fragmentation index of the samples were 33.6 ± 2 % and 27.2 ± 1.2, respectively. The prevalence of HCMV was 11%, and there was no statistically significant difference between HCMV and sperm parameters except the motility. Conclusions: HCMV infection could be important in sperm motility, and HCMV prevalence in infertile patients was 11%. Further investigations in this field can be helpful for a clear result in the future.

P–109 Comparison between the outcome of sperm vitrification protocol and conventional slow freezing protocol for semen cryopreservation

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
K Patel ◽  
N Sharma ◽  
V Mishra ◽  
R Aggarwal ◽  
A Suthar ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Survival Rates ◽  
Semen Sample ◽  
Slow Freezing ◽  
Cellular Damage ◽  
Fragmentation Index ◽  
Significant Difference ◽  
Group 2 ◽  
Dna Fragmentation Index ◽  
Group 1

Abstract Study question Does sperm vitrification technique helps in increasing sperm survival and low DNA fragmentation index post warming. Summary answer Sperm vitrification protocol results in better motility, high progression and low DNA fragmentation index as compared to slow freezing. What is known already Cryopreservation is ceasing and resuming the cell metabolism, which can be achieved by different techniques like slow freezing and vitrification .Vitrification allows solidification of the cells and extracellular milieu into a glass like state without formation of ice which protects intracellular and extracellular ice formation, and further helps in avoiding different types of cryo-injuries and cellular damage. Study design, size, duration: Comparative study from July 2019 to Oct 2020 in IVF unit of IKDRC Hospital. Two hundred and ten patients were randomized by computer generated list and divided into two groups. Group 1 (n = 110) samples cryopreserved by vitrification and Group 2 (n = 100) samples cryopreserved by conventional slow freezing. Participants/materials, setting, methods Semen sample were analyzed by WHO 2010 laboratory manual, including all normozoospermic samples , other abnormal samples were excluded from the study . Method of semen preparation before cryopreservation is similar for both the groups, double density gradient method of preparation was used . Semen sample with high viscosity, hypo and hyper-spermia were also excluded. Similar cryovials of 2ml volume were used for both groups. Main results and the role of chance In group 1 where samples were cryopreserved by vitrification sperm motility was (54.3% vs 49.2%)vs in group 2 where samples were cryopreserved by slow freezing , non- significant difference were observed , but progressive motility was significantly higher in group 1 as compared to group 2 (36.8%vs17.9%) and DNA fragmentation index is significantly lower in group 1 vitrification than in group 2slow freezing ( 9.7% vs 20%). Limitations, reasons for caution Technical proficiency of the operator to avoid human errors and still larger randomized control studies are needed to strengthen these results Wider implications of the findings: Our study demonstrates that vitrification is better than slow freezing of human sperm, improved survival rates with high progression were found with vitrification and low DNA fragmentation index were also observed in samples cryopreserved with vitrification protocol. Trial registration number Not applicable

Expression of PARP1 in infertile men and correlation with DNA fragmentation index

2012 ◽  
Vol 98 (3) ◽  
pp. S241
Author(s):  
S. Mishra ◽  
K. Mohanty ◽  
J. Pani ◽  
T. Hasan ◽  
R. Kumar ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Infertile Men ◽  
Fragmentation Index ◽  
Dna Fragmentation Index

scholarly journals Evaluation of the Relationship Between Body Mass Index (BMI) and DNA Fragmentation Index Changes in Primary Infertile Patients Following Microscopic Sub Inguinal Varicocelectomy

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Mohamadreza Dadfar ◽  
Alireza Kheradmand ◽  
Hayat Mombeini ◽  
Javad Mohammadi Asl ◽  
Abbas Mahdavian
Keyword(s):  
Body Mass Index ◽  
Dna Fragmentation ◽  
Body Mass ◽  
Normal Weight ◽  
Weight Group ◽  
Normal Weight Group ◽  
Fragmentation Index ◽  
Significant Difference ◽  
Infertile Patients ◽  
Dna Fragmentation Index

Objectives: To investigate changes in DNA fragmentation index in primary infertile patients with varicocele, which is followed by microscopic subingual varicocelectomy in different groups based on body mass index (BMI). Methods: This study was performed in 100 patients with primary infertility with varicocele. Patients were divided into three groups (normal (N), overweight (OW), and obese (OB)) based on BMI index. DNA fragmentation index (DFI) parameters were evaluated before and 6 months after varicocelectomy. For DFI analysis, the SCD (sperm chromatin dispersion test) method was used. Data were analyzed using t-test, Chi-square, and ANOVA. Results: In this study, the mean age of participants was 33.6 and their mean BMI was 28.6, that 51 patients underwent bilateral varicocelectomy and 49 patients underwent left varicocelectomy surgery. In this study, a comparison of DFI before and 6 months after surgery showed a decrease in DFI in all three groups. The difference was 23 in the normal weight group, 11.2 in the overweight group and 9.58 in the obese group, which is statistically significant (PV < 0.05). Also, in comparison with the rate of DFI reduction between groups, the normal weight group showed a greater decrease than the overweight and obese group. This difference was statistically significant (PV < 0.05), while comparing the rate of DFI reduction between the two groups of overweight and obese, was observed no significant difference (PV = 0.635). Conclusions: Although DFI level decreased significantly 6 months after surgery in all groups with different body mass index. However, the rate of reduction was not the same in different groups and was higher in normal-weight patients than in overweight and obese individuals. But there was no significant difference in the rate of reduction between the overweight and obese groups.

scholarly journals In vitro Alpha Lipoic Acid supplementation in freeze-dried human sperm: the impact on DNA fragmentation index

2021 ◽  
Vol 5 (1) ◽  
pp. 125
Author(s):  
Rezia Octarina ◽  
M. P. B. Dyah Pramesti ◽  
Agustinus Agustinus
Keyword(s):  
Dna Fragmentation ◽  
Lipoic Acid ◽  
Freeze Drying ◽  
Human Spermatozoa ◽  
Alpha Lipoic Acid ◽  
Fragmentation Index ◽  
Freeze Dried ◽  
Significant Difference ◽  
The Impact ◽  
Dna Fragmentation Index

Freeze-drying technique is an alternative method on sperm preservation. However, this process can results in DNA damage. Adding antioxidants before freezing can reduce the detrimental effects of ROS on spermatozoa. Alpha lipoic acid (ALA) is one of the potent antioxidant that can be used to protect the sperm DNA. This study was aimed to determine the effect of ALA supplementation before freeze-drying on the DNA fragmentation index (DFI) of human spermatozoa. Nine semen samples were collected and evaluated for DFI (O1) then divided into 3 groups; freeze-drying without ALA (O2), freeze-drying with ALA 1,25 mg (O3) and freeze-drying with ALA 2,5 mg (O4).All three groups were stored at 4oC for 1 week. Sperm DFI were evaluated before and after freeze-drying. The result of this study showed a significant increase in sperm DFI in all three groups after freeze-drying (p<0,05) when compared to DFI before freeze-drying (14,67 ±3,295). However there was no significant difference between O2 (26,90 ±9,180) and O4 (29,29 ±5,524) group where the supplementation of 2,5 mg ALA did not have significant effect on protecting the DNA of freeze-dried human spermatozoa. The highest DFI was in the O3 group (44,64 ±11,717). Therefore, ALA supplementation before freeze-drying does not have a significant effect on protecting the DNA of freeze-dried human spermatozoa.

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