P–109 Comparison between the outcome of sperm vitrification protocol and conventional slow freezing protocol for semen cryopreservation

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
K Patel ◽  
N Sharma ◽  
V Mishra ◽  
R Aggarwal ◽  
A Suthar ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Survival Rates ◽  
Semen Sample ◽  
Slow Freezing ◽  
Cellular Damage ◽  
Fragmentation Index ◽  
Significant Difference ◽  
Group 2 ◽  
Dna Fragmentation Index ◽  
Group 1

Abstract Study question Does sperm vitrification technique helps in increasing sperm survival and low DNA fragmentation index post warming. Summary answer Sperm vitrification protocol results in better motility, high progression and low DNA fragmentation index as compared to slow freezing. What is known already Cryopreservation is ceasing and resuming the cell metabolism, which can be achieved by different techniques like slow freezing and vitrification .Vitrification allows solidification of the cells and extracellular milieu into a glass like state without formation of ice which protects intracellular and extracellular ice formation, and further helps in avoiding different types of cryo-injuries and cellular damage. Study design, size, duration: Comparative study from July 2019 to Oct 2020 in IVF unit of IKDRC Hospital. Two hundred and ten patients were randomized by computer generated list and divided into two groups. Group 1 (n = 110) samples cryopreserved by vitrification and Group 2 (n = 100) samples cryopreserved by conventional slow freezing. Participants/materials, setting, methods Semen sample were analyzed by WHO 2010 laboratory manual, including all normozoospermic samples , other abnormal samples were excluded from the study . Method of semen preparation before cryopreservation is similar for both the groups, double density gradient method of preparation was used . Semen sample with high viscosity, hypo and hyper-spermia were also excluded. Similar cryovials of 2ml volume were used for both groups. Main results and the role of chance In group 1 where samples were cryopreserved by vitrification sperm motility was (54.3% vs 49.2%)vs in group 2 where samples were cryopreserved by slow freezing , non- significant difference were observed , but progressive motility was significantly higher in group 1 as compared to group 2 (36.8%vs17.9%) and DNA fragmentation index is significantly lower in group 1 vitrification than in group 2slow freezing ( 9.7% vs 20%). Limitations, reasons for caution Technical proficiency of the operator to avoid human errors and still larger randomized control studies are needed to strengthen these results Wider implications of the findings: Our study demonstrates that vitrification is better than slow freezing of human sperm, improved survival rates with high progression were found with vitrification and low DNA fragmentation index were also observed in samples cryopreserved with vitrification protocol. Trial registration number Not applicable

Molecular Detection of Human Cytomegalovirus in Semen of infertile Men in Tehran

2021 ◽  
Author(s):  
Maryam Moradi ◽  
Somayeh Sadat Moochani ◽  
Nazila Yamini ◽  
Davod Javanmard ◽  
Arezoo Marjani ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Human Cytomegalovirus ◽  
Medical Sciences ◽  
Sexually Transmitted ◽  
Infertile Men ◽  
Fragmentation Index ◽  
Significant Difference ◽  
Clear Result ◽  
The Mean ◽  
Dna Fragmentation Index

Background and Aims: Human cytomegalovirus (HCMV) can induce latency and evade the immune system. The latent virus can reactive later in older ages and due to immunosuppressive conditions. Sexually transmitted diseases and viruses can influence the male reproductive system, and members of the Herpesviridae family are one of these important viruses. Regarding the importance of herpesviruses, specially HCMV, this research aimed to evaluate the prevalence of HCMV in semen samples of infertile men. Materials and Methods: Semen samples were collected from infertility centers affiliated to Iran University of Medical Sciences. The chromatin state was assessed by DNA fragmentation index. Nucleic acids were extracted from the semen specimens, and a real-time polymerase chain reaction assay was performed to detect HCMV DNA. Results: Enrolled patients were 82 infertile men. The mean age of participants was 37.3 ± 6.1 years, and the mean motility and DNA fragmentation index of the samples were 33.6 ± 2 % and 27.2 ± 1.2, respectively. The prevalence of HCMV was 11%, and there was no statistically significant difference between HCMV and sperm parameters except the motility. Conclusions: HCMV infection could be important in sperm motility, and HCMV prevalence in infertile patients was 11%. Further investigations in this field can be helpful for a clear result in the future.

Primary or secondary Bentall-De Bono procedure: are the outcomes worse?

2019 ◽  
Vol 27 (4) ◽  
pp. 271-277
Author(s):  
Ameya Kaskar ◽  
Deepak V Bohra ◽  
Rahul Rao K ◽  
Varun Shetty ◽  
Devi Shetty
Keyword(s):  
Propensity Score ◽  
Hospital Mortality ◽  
Propensity Score Matching ◽  
Survival Rates ◽  
Median Sternotomy ◽  
Postoperative Mortality ◽  
Mortality And Morbidity ◽  
Significant Difference ◽  
Group 2 ◽  
Group 1

Background The aim of this study was to compare the outcomes of a primary and secondary Bentall-De Bono procedure. Methods From 2008 to 2015 (8-year period), 308 patients underwent a Bentall-De Bono procedure in our institute. The mean age was 43 ± 13 years and 80% were men. Twenty-eight patients had prior cardiac surgery through a median sternotomy (group 1) and 280 underwent a primary Bentall-De Bono procedure (group 2). Various preoperative and perioperative parameters were analyzed before and after propensity-score matching. Results Before propensity-score matching, patients undergoing a secondary Bentall-De Bono procedure had a worse preoperative profile, as indicated by a higher EuroSCORE II ( p < 0.0001), with hospital mortality in group 1 of 14% (4/28) and 5% (14/280) in group 2 ( p = 0.069). After propensity-score matching, there was no significant difference in EuroSCORE II ( p = 0.922) or hospital mortality ( p = 0.729). After adjusting for the different variables, repeat sternotomy could not be identified as an independent predictor of postoperative mortality or morbidity. Survival at the end of 1 and 5 years in both groups showed no significant differences before or after propensity-score matching ( p = 0.328 and p = 0.356, respectively). In Cox multivariable regression analysis, reoperation was not identified as an independent factor for survival before ( p = 0.559) or after propensity-score matching ( p = 0.365). Conclusion A secondary Bentall-De Bono procedure can be performed with acceptable mortality and morbidity, and with midterm survival rates comparable to those of a primary Bentall-De Bono procedure.

scholarly journals Evaluation of the Relationship Between Body Mass Index (BMI) and DNA Fragmentation Index Changes in Primary Infertile Patients Following Microscopic Sub Inguinal Varicocelectomy

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Mohamadreza Dadfar ◽  
Alireza Kheradmand ◽  
Hayat Mombeini ◽  
Javad Mohammadi Asl ◽  
Abbas Mahdavian
Keyword(s):  
Body Mass Index ◽  
Dna Fragmentation ◽  
Body Mass ◽  
Normal Weight ◽  
Weight Group ◽  
Normal Weight Group ◽  
Fragmentation Index ◽  
Significant Difference ◽  
Infertile Patients ◽  
Dna Fragmentation Index

Objectives: To investigate changes in DNA fragmentation index in primary infertile patients with varicocele, which is followed by microscopic subingual varicocelectomy in different groups based on body mass index (BMI). Methods: This study was performed in 100 patients with primary infertility with varicocele. Patients were divided into three groups (normal (N), overweight (OW), and obese (OB)) based on BMI index. DNA fragmentation index (DFI) parameters were evaluated before and 6 months after varicocelectomy. For DFI analysis, the SCD (sperm chromatin dispersion test) method was used. Data were analyzed using t-test, Chi-square, and ANOVA. Results: In this study, the mean age of participants was 33.6 and their mean BMI was 28.6, that 51 patients underwent bilateral varicocelectomy and 49 patients underwent left varicocelectomy surgery. In this study, a comparison of DFI before and 6 months after surgery showed a decrease in DFI in all three groups. The difference was 23 in the normal weight group, 11.2 in the overweight group and 9.58 in the obese group, which is statistically significant (PV < 0.05). Also, in comparison with the rate of DFI reduction between groups, the normal weight group showed a greater decrease than the overweight and obese group. This difference was statistically significant (PV < 0.05), while comparing the rate of DFI reduction between the two groups of overweight and obese, was observed no significant difference (PV = 0.635). Conclusions: Although DFI level decreased significantly 6 months after surgery in all groups with different body mass index. However, the rate of reduction was not the same in different groups and was higher in normal-weight patients than in overweight and obese individuals. But there was no significant difference in the rate of reduction between the overweight and obese groups.

scholarly journals In vitro Alpha Lipoic Acid supplementation in freeze-dried human sperm: the impact on DNA fragmentation index

2021 ◽  
Vol 5 (1) ◽  
pp. 125
Author(s):  
Rezia Octarina ◽  
M. P. B. Dyah Pramesti ◽  
Agustinus Agustinus
Keyword(s):  
Dna Fragmentation ◽  
Lipoic Acid ◽  
Freeze Drying ◽  
Human Spermatozoa ◽  
Alpha Lipoic Acid ◽  
Fragmentation Index ◽  
Freeze Dried ◽  
Significant Difference ◽  
The Impact ◽  
Dna Fragmentation Index

Freeze-drying technique is an alternative method on sperm preservation. However, this process can results in DNA damage. Adding antioxidants before freezing can reduce the detrimental effects of ROS on spermatozoa. Alpha lipoic acid (ALA) is one of the potent antioxidant that can be used to protect the sperm DNA. This study was aimed to determine the effect of ALA supplementation before freeze-drying on the DNA fragmentation index (DFI) of human spermatozoa. Nine semen samples were collected and evaluated for DFI (O1) then divided into 3 groups; freeze-drying without ALA (O2), freeze-drying with ALA 1,25 mg (O3) and freeze-drying with ALA 2,5 mg (O4).All three groups were stored at 4oC for 1 week. Sperm DFI were evaluated before and after freeze-drying. The result of this study showed a significant increase in sperm DFI in all three groups after freeze-drying (p<0,05) when compared to DFI before freeze-drying (14,67 ±3,295). However there was no significant difference between O2 (26,90 ±9,180) and O4 (29,29 ±5,524) group where the supplementation of 2,5 mg ALA did not have significant effect on protecting the DNA of freeze-dried human spermatozoa. The highest DFI was in the O3 group (44,64 ±11,717). Therefore, ALA supplementation before freeze-drying does not have a significant effect on protecting the DNA of freeze-dried human spermatozoa.

P–040 Correlation between DNA fragmentation index and big halo pattern with sperm decondensation index and semen sample parameters

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
A D Crippa ◽  
M C Magli ◽  
A P Ferraretti ◽  
L Gianaroli
Keyword(s):  
Dna Fragmentation ◽  
Negative Correlation ◽  
Reproductive Potential ◽  
Semen Sample ◽  
Dna Integrity ◽  
Progressive Motility ◽  
Fragmented Dna ◽  
Fragmentation Index ◽  
Sperm Dna ◽  
Dna Fragmentation Index

Abstract Study question Does sperm DNA integrity evaluated by DNA fragmentation index (DFI) and big halo pattern correlate with sperm decondensation index (SDI) and semen sample parameters? Summary answer DFI correlates with SDI and semen sample parameters in a stronger way than the big halo pattern What is known already The sperm chromatin dispersion test evaluates DNA integrity by measuring the susceptibility of sperm DNA containing breaks to denature when treated by an acid solution. Spermatozoa with intact DNA produce big or medium size halos of dispersed DNA loops, whereas small halos or no halos indicate fragmented DNA. The DFI calculates the proportion of spermatozoa with fragmented DNA. Data have been published documenting the negative effect of sperm DFI on embryo viability, suggesting that its evaluation could contribute to the prediction of the male reproductive potential Study design, size, duration A prospective study between 2011 to 2019 included 300 patients attending our clinic for fertility treatment. All sperm samples were analyzed according to WHO criteria, and the results from the DNA integrity analysis were related to the semen sample indices Participants/materials, setting, methods Of the 300 males included in the study, 118 were normozoospermic, 16 were oligozoospermic (O), 63 were asthenozoospermic (A), 9 were teratozoospermic, 7 were AT, 51 were OA, 5 were OT, and 31 OAT. The DNA integrity was assessed by the Halosperm test, and DNA decondensation by the aniline blue assay. A big halo was defined as a dispersion greater or equal to the length of the minor diameter of the core Main results and the role of chance DFI showed negative correlations with progressive motility (r= –0.532, p = 2.816 E–23), total motility (r= –0.598, p = 1.688 E–30) and morphology (r= –0.338, p = 2.954 E–9). Accordingly, when compared with normozoospermic, DFI was significantly higher in A and T samples (29.5 ±12.0 and 36.5±4.8 respectively, p &lt; 0.002) with the highest levels found in samples with combined defects (45.2±12.5 in AT, p &lt; 0.002; 51.3±17.2 in OAT, p &lt; 0.002). DFI also showed a negative correlation with the big halo pattern (r= –0.656, p = 2.934 E–38) and a positive correlation with the SDI (r = 0.429, p = 7.314 E–15). For the big halo, negative correlations were found with progressive motility (r = 0.429, p = 7.314 E–15) and morphology (r = 0.407, p = 4.077 E–13) resulting in a lower incidence in T samples (27.0±9.6, p &lt; 0.002) that was especially relevant in AT (18.3±14.5, p &lt; 0.002), OT (33.0±10.2, p &lt; 0.02) and OAT samples (20.6±15.8, p &lt; 0.002). SDI presented a negative correlation with total motility (r= –0.403, p = 3.849 E–13) and was fond to be increased in A samples (32.4±11.8, p &lt; 0.002) as well as in samples with double defects (38.9±19.2 in AT samples and 38.8±15.9 in OA samples, p &lt; 0.002) and triple defects (42.6±16.8 in OAT, p &lt; 0.002) Limitations, reasons for caution The study did not evaluate the lifestyle and reproductive history of the patients Wider implications of the findings: Although the effects of sperm DNA damage on reproductive outcomes are still unclear, the correlation between sperm DNA fragmentation, semen parameters and reproductive potential is emerging. DFI, big halo and SDI could contribute to the diagnosis of male infertility especially in categories of patients with poor prognosis of pregnancy. Trial registration number Not applicable

In-vivo evaluation of the effect of cyanoacrylate on prosthetic vascular graft infection – does cyanoacrylate increase the severity of infection?

VASA ◽  
2020 ◽  
Vol 49 (4) ◽  
pp. 281-284
Author(s):  
Atıf Yolgosteren ◽  
Gencehan Kumtepe ◽  
Melda Payaslioglu ◽  
Cuneyt Ozakin
Keyword(s):  
Vascular Graft ◽  
Graft Infection ◽  
Vascular Graft Infection ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Prosthetic Vascular Graft Infection ◽  
Group 1

Summary. Background: Prosthetic vascular graft infection (PVGI) is a complication with high mortality. Cyanoacrylate (CA) is an adhesive which has been used in a number of surgical procedures. In this in-vivo study, we aimed to evaluate the relationship between PVGI and CA. Materials and methods: Thirty-two rats were equally divided into four groups. Pouch was formed on back of rats until deep fascia. In group 1, vascular graft with polyethyleneterephthalate (PET) was placed into pouch. In group 2, MRSA strain with a density of 1 ml 0.5 MacFarland was injected into pouch. In group 3, 1 cm 2 vascular graft with PET piece was placed into pouch and MRSA strain with a density of 1 ml 0.5 MacFarland was injected. In group 4, 1 cm 2 vascular graft with PET piece impregnated with N-butyl cyanoacrylate-based adhesive was placed and MRSA strain with a density of 1 ml 0.5 MacFarland was injected. All rats were scarified in 96th hour, culture samples were taken where intervention was performed and were evaluated microbiologically. Bacteria reproducing in each group were numerically evaluated based on colony-forming unit (CFU/ml) and compared by taking their average. Results: MRSA reproduction of 0 CFU/ml in group 1, of 1410 CFU/ml in group 2, of 180 200 CFU/ml in group 3 and of 625 300 CFU/ml in group 4 was present. A statistically significant difference was present between group 1 and group 4 (p < 0.01), between group 2 and group 4 (p < 0.01), between group 3 and group 4 (p < 0.05). In terms of reproduction, no statistically significant difference was found in group 1, group 2, group 3 in themselves. Conclusions: We observed that the rate of infection increased in the cyanoacyrylate group where cyanoacrylate was used. We think that surgeon should be more careful in using CA in vascular surgery.

Tuberculosis of the meninges and central nervous system. Experience of clinical diagnostics in St. Petersburg for 50 years

MedAlliance ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 14-24
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Medical Errors ◽  
Clinical Diagnostics ◽  
Lethal Outcome ◽  
Significant Difference ◽  
Group 2 ◽  
Allergic Vasculitis ◽  
Tuberculosis Meningitis ◽  
Group 1

The clinic and diagnostics of tuberculosis meningitis (TM) in 926 patients treated in St. Petersburg hospitals in 1965–1994 (group 1) and in 1995–2018 (group 2) is presented. The TM clinic is demonstrated to be determined by the form of tuberculosis and its characteristic generalization nature in the presence of repeated waves of bacteremia and allergic vasculitis of greater or lesser severity. There is clinical peculiarity of TM in primary pulmonary tuberculosis and its early large-focal and late miliar generalization, as well as in hematogenous tuberculosis. In patients of the 1st and 2nd groups the TM clinic shows in some respects a noticeable similarity, in others — a significant difference. Despite the typical symptoms, early diagnosis of TM took place in less than 20% of patients. Clinical examples illustrating the unusual development of TM, contrasting with its usual course, are given. A number of objective and subjective factors contributing to the adverse evolution of TM and its lethal outcome are discussed. These include the peculiarity of modern tuberculosis, especially when associated with HIV infection, as well as medical errors associated with ignorance of the pathogenesis of tuberculosis and failure to comply with the minimum examination for tuberculosis.

Two Adhesive Systems-effect on Adhesion to Tooth Structure

2019 ◽  
Vol 70 (7) ◽  
pp. 2608-2613
Author(s):  
Larisa Simona Deac ◽  
Kamel Earar ◽  
Adela Loredana Colceriu Burtea ◽  
Alexandra Stefania Berghe ◽  
Aurora Antoniac ◽  
...  
Keyword(s):  
Adhesive Systems ◽  
Hybrid Layer ◽  
Tooth Structure ◽  
Selective Etch ◽  
Etch And Rinse ◽  
Significant Difference ◽  
Wisdom Teeth ◽  
Group 2 ◽  
Self Etch ◽  
Group 1

This study evaluates and compares by dye penetration method and SEM photomicrographs the sealing obtained using two different classes of adhesive systems (etch-and-rinse and self-etch with selective etching) with SDR (Dentsply) bulk fill composite. 84 class V cavities were prepared on oral and vestibular face of 42 intact, freshly extracted wisdom teeth. The cavities were randomly divided in two groups and restored: Group 1 with prime &bond one select (Dentsply) and SDR (Dentsply) and Group 2 with prime&bond one Etch&Rinse (Dentsply) and SDR (Dentsply). Prime&bond one Select (Dentsply) is a single component adhesive and can be used in self etch mode, in selective enamel etch mode, or in etch-and-rinse mode. We chosen for this study the selective etch of the enamel mode. Prime&bond one Etch Rinse (Dentsply) is a universal etch-and-rinse one-bottle dental adhesive, designed to be used in two steps. The bulk fill composites are commonly used in modern dentistry due to their properties of low polymerization shrinkage and curing in layer of 4 mm depth, offering the practitioner a fast clinical procedure with good results. The results showed a good sealing at enamel and dentin margins with no statistically significant difference between adhesives, even though the mean of enamel infiltration was smaller for Group1. Furthermore the results show that there were differences between the two groups, for the infiltrations at the enamel, the values of microleakage being arithmetically higher for Group 1, but with no statistically difference between the two groups.SEM images showed for both groups a good adhesion surface with the tooth, but the hybrid layer of the total-etch adhesives is different from the hybrid layer formed by self etch adhesives, in terms of thickness, uniformity. In conclusion both adhesive systems have equivalent sealing qualities and can be successfully used with SDR.

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