scholarly journals Assessment Synergistic Effects of Integrated Therapy with Epigallocatechin-3-Gallate (EGCG) & Arsenic Trioxide and Irradiation on Breast Cancer Cell Line

2020 ◽  
Author(s):  
Vahid CHANGIZI ◽  
Samayeh AZARIASL ◽  
Elahe MOTEVASELI ◽  
Saeedeh JAFARI NODOOSHAN
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Arsenic Trioxide ◽  
Green Tea ◽  
Mtt Assay ◽  
Low Dose ◽  
Synergistic Effects ◽  
Integrated Therapy ◽  
Low Dose Chemotherapy ◽  
Mcf 7

Background: Breast cancer is the most common invasive malignancy among women in the world. The current breast cancer therapies pose significant clinical challenges. Low-dose chemotherapy represents a new strategy to treat solid tumors in combination with natural products such as green tea catechins. Epigallocatechin-3-gallate (EGCG) is the major polyphenolic extract from green tea with potent anticancer and antioxidant effects. The purpose of this study was to investigate the effects of EGCG, Arsenic trioxide (ATO) and gamma radiation on MCF-7 cell line. Methods: The anti-proliferative effects of EGCG and ATO individually, moreover in combination with radiation on MCF-7 cells were evaluated with MTT assay. The expression of apoptotic gens (Bax, Bcl-2, Caspase-3 and Fas) was assessed by real-time PCR. Results: Based on the results of MTT assay, EGCG and ATO exhibited dose and time-dependent antiproliferative effects on MCF-7 cells. The combined therapy of EGCG and ATO in presence and absence radiation could rise cell death up to 80%. Moreover, integrated therapy made Bax up-regulated and Bcl-2 downregulated. Conclusion: In assessment synergistic effects of integrated therapy with EGCG and ATO and irradiation had been significant impact on low dose chemotherapy for breast cancer treatment.

scholarly journals Synthesis of 1-[(Aryl)(3-amino-5-oxopyrazolidin-4-ylidene) methyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic Acid Derivatives and Their Breast Anticancer Activity

Crystals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 571
Author(s):  
Ahmed Gaber ◽  
Walaa F. Alsanie ◽  
Majid Alhomrani ◽  
Abdulhakeem S. Alamri ◽  
Ibrahim M. El-Deen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Carboxylic Acid ◽  
Anticancer Activity ◽  
Cell Line ◽  
Mtt Assay ◽  
Analytical Techniques ◽  
Anticancer Effect ◽  
Reference Compound ◽  
Anticancer Effects ◽  
Mcf 7

This research aimed to produce new 1-[(aryl)(3-amino-5-oxopyrazolidin-4-ylidene) methyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic acid derivatives and check their anticancer effect against the breast cancer MCF-7 cell line. The 2-oxo-1,2-dihydroquinoline-3-carboxylic acid (4) compound was obtained by hydrolyzing ethyl 2-oxo-1,2-dihydroquinoline-3-carboxylate (2) with thiourea and anhydrous potassium carbonate ethanol, which was then treated with ethyl 3-substituted 2-cyanoacrylates (6) in the presence of triethylamine in diethyl formamide to give 1-[2-(ethoxy)carbonyl-2-cyano-1-arylvinyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic (7a,d). Cyclization of compound 7 with hydrazine hydrate ethanol inferred the association of 1-[(aryl)(3 amino-5-oxopyrazolidin-4-ylidene)methyl-2-oxo-1,2-dihydroquinol-3-carboxylates (8a,d). Spectroscopic and micro-analytical techniques such as IR, NMR, and elemental analysis were used to validate the structure of the synthesized organic compounds. The anticancer effects of the synthesized compounds 7a–d and 8a–d were tested by using the MTT assay on the MCF-7 cell line. When compared to the reference compound Dox, the compounds 7b, 7c, 8a, 8b, and 8c demonstrated strong anticancer activity against the MCF-7 cell line. The anticancer effects of the synthesized compounds 7a–d and 8a–d were tested against the MCF-7 cell line, using MTT assay. The compounds 7b, 7c, 8a, 8b, and 8c showed significant anticancer activity compared to the reference compound Dox against the MCF-7 cell line.

Synergistic Effects of Bortezomib with Low Dose Melphalan Against Human AML Cells.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 4463-4463
Author(s):  
Marc Gautier ◽  
Jeffrey A. Bubis ◽  
Jia-Yan Wu ◽  
Kenneth R. Meehan
Keyword(s):  
Clinical Trial ◽  
Cell Line ◽  
Mtt Assay ◽  
Low Dose ◽  
Synergistic Effects ◽  
The Elderly ◽  
Resistant Disease ◽  
Elderly Aml ◽  
Potential Synergy ◽  
Dilution Experiments

Abstract The treatment of AML in the elderly population is difficult given the inherent resistant disease, the toxic effects of therapy and the presence of co-morbid conditions. We proposed preclinical studies to investigate the potential synergy of bortezomib with melphalan against AML cells, with the anticipation of developing a clinical trial. To test the killing potential of bortezomib or melphalan, limiting dilution experiments were performed with subsequent analysis using the MTT assay. Two human AML cell lines, GDM-1 and Kasumi-1, were used as targets. Tumor cells (7.5 x 103 cells/well) were plated on 96-well tissue culture plates and incubated overnight at 37 °C with 5% CO2. At 24 hrs, varying concentrations of melphalan (doses: 100 uM to 1 uM) or bortezomib (doses: 100 nM to 1 nM) were added to each well. After 48 hrs of culture, the MTT assay was performed. Each test was run in triplicate. When using the GDM-1 cell line, the addition of melphalan alone (1 uM) resulted in 80% viability (+/− 1.7%), while bortezomib alone (1 nM) yielded 86% viability (+/− 3.6%). An increased dose of each medication decreased the viability of the GDM-1 cells. An increased dose of Melphalan (3 uM) reduced the viability to 23% (+/− 4.2 %). The viability dropped to 62% (+/− 4.9 %) with an increased dose of Bortezomib (3 nM). After combining the medications, the inhibitory activity against the GDM-1 cells required lower doses then either drug alone. For example, melphalan (1uM) with bortezomib (1 nM) reduced the viability of GDM-1 cells to 20% (+/− 2.8%); While melphalan (3 uM) and bortezomib (3 nM) further suppressed the viability to 3.7 % (+/−2.5 %). Similar results were found using the Kasumi-1 AML cell line. These results demonstrate the potential synergy of the combination of bortezomib with melphalan against AML cells. These preclinical results are currently being tested in a clinical trial using low dose melphalan with bortuzimab in elderly AML and MDS patients.

Synergistic effect between doxorubicin and a low dose of all-trans-retinoic acid in MCF-7 breast cancer cell line

1997 ◽  
Vol 116 (1) ◽  
pp. 103-110 ◽  
Author(s):  
Salvatore Toma ◽  
Giuseppina Maselli ◽  
Giuseppe Dastoli ◽  
Elena De Francisci ◽  
Patrizia Raffo
Keyword(s):  
Breast Cancer ◽  
Retinoic Acid ◽  
Synergistic Effect ◽  
Cell Line ◽  
Breast Cancer Cell Line ◽  
Low Dose ◽  
Cancer Cell Line ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid ◽  
Mcf 7

scholarly journals The Effect of Piperine on MMP-9, VEGF, and E-cadherin Expression in Breast Cancer MCF-7 Cell Line

2021 ◽  
Author(s):  
Zahra Zare ◽  
Tina Nayerpour Dizaj ◽  
Armaghan Lohrasbi ◽  
Zakieh Sadat Sheikhalishahi ◽  
Mohammad Panji ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Mtt Assay ◽  
New Drugs ◽  
Piper Nigrum ◽  
Migration And Invasion ◽  
Control Group ◽  
Mrna Levels ◽  
Mcf 7 ◽  
E Cadherin

Background: Vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9), and E-cadherin play a vital role in the behavior of angiogenesis, metastasis, and invasion of breast tumor cells. Piperine, the main component of Piper Nigrum, has shown anti-cancer properties in various malignancies. This Study investigates the potential effect of piperine on MMP-9, E-cadherin, and VEGF expression in breast cancer MCF-7 cell line. Methods: MTT assay was applied to assess the viability of MCF-7 cells. The mRNA levels of MMP-9, VEGF, and E-cadherin were assayed by qRT-PCR. Western blot was performed to identify the protein level of MMP-9. Results: MTT assay results showed that piperine treatment (5, 10, 25, 50, 75, and 100 μM) for 24 hours effectively inhibited cell viability of MCF-7 cells as compared with the control group. Furthermore, the gene expression of VEGF, MMP-9, and E-cadherin was dose-dependently suppressed by piperine treatment (5, 10 and 25 μM) (P<0.05; P<0.01). The results also indicated that piperine (5, 10, and 25 μM) significantly suppressed MMP-9 protein expression after 24 hours of piperine treatment (P<0.01). Conclusion: These results suggest that piperine may prevent angiogenesis, migration, and invasion of MCF-7 cells by suppressing MMP-9 and VEGF, and by inducing E-cadherin expression. Hence, it may be a suitable candidate for designing new drugs in cancer therapy.

scholarly journals Anticancer Activity Of Silver Nanoparticles Synthesized Using Extract of Animal Waste As A Traditional Medicine On MCF-7 Human Breast Cancer Cell Line

2021 ◽  
Author(s):  
Mohammad Mousavi-Khattat ◽  
Hamid Nourbakhshan ◽  
Mehrnaz Roumi ◽  
Mahshid Ebrahiminejad ◽  
Yasaman Fazeli ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Anticancer Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Mtt Assay ◽  
Synthesis Methods ◽  
Mcf 7

Abstract Green synthesis methods are environmentally friendly, cost effective and nonhazardous for biomedical applications in comparison with other methods. The aim of the study was green synthesis of silver nanoparticles using medicinal animal dung extract as a reducing, capping and stabilizing agent for the first time among other synthesis methods of silver nanoparticles. Female donkey’s dung was capable to reduce silver ions to nanoparticles and stabilize them. Silver nanoparticles with average sizes of 36 nm were synthesized and characterized by UV-Vis, FT-IR, XRD and TEM. Moreover, synthesized nanoparticles were analyzed in terms of anticancer activity by MTT assay on MCF-7 cell line. UV–Visible spectrophotometer showed an absorbance peak in the range of 414-433 nm. To identify the phytochemical coating of particles, FTIR analysis was used. Transmission electron microscope (TEM) images and X-ray diffraction (XRD) confirmed the formation of small spherical silver nanoparticles. The MTT assay revealed potent anticancer effects of the aqueous extract synthesized nanoparticles on MCF-7 cells, incubated for 24 hours. Based on the current findings, it is strongly believing that the use of donkey’s dung offers large scale production of biocompatible silver nanoparticles that can be suggested to possess valuable anticancer agents against breast cancer cell lines.

scholarly journals APRICOXIB

2018 ◽  
Vol 25 (12) ◽  
pp. 1915-1922
Author(s):  
Fatima Rizvi ◽  
Syed Mahboob Alam ◽  
Farah Asad ◽  
Hina Shams
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cell Lines ◽  
Mtt Assay ◽  
Cytotoxic Effects ◽  
Inflammatory Conditions ◽  
Ic50 Value ◽  
Ht 29 ◽  
Mcf 7

Background: Breast cancer is most frequently diagnosed cancer globally but there is not any ideal economical and safer agent that not only decreases the progression but also resolve complexities associated with breast cancer such as inflammatory conditions. There was strong link between inflammation and cancer specially breast cancer. Thus by inhibiting the COX enzyme may inhibit the progression of cancer beside of its role in inflammatory conditions of breast. Study Design: Interventional In Vitro trial. Setting: Department of Pharmacology in alliance with PCMD. Period: The duration of study from April 2016 to February 2017. Methodology: For this purpose we used five cancerous lines MCF-7, MDA-MB-231, MCF-10, HT-29 and Hela cell lines. For demonstrating the cytotoxic effects of Apricoxib we used MTT assay (for all cell Lines) and Trypan blue dye exclusion assay (Primarily for MCF-7 cell lines). For calculation of minimum dose required for exert cytotoxic effects of Apricoxib and its selectivitytowards cancerous cells of breast tissue we calculated its IC50 value and Selectivity Index (SI) by MTT assay. Results: Apricoxib significantly reduce the viability of MCF-7, MDA-MB-231, Hela, HT-29 as assessed by MTT assay in dose dependent manner (χ2 (2) = 26.483, p<0.001), (χ2 (2) = 26.49, p<0.001), (χ2 (2) = 26.062, p<0.001) and (χ2 (2) = 26.062, p<0.001) respectively. However Apricoxib had non-significant effects on % viability of MCF-10 cell line (χ2 (2) = 4.167, p=0.654) as assessed by MTT assay. Furthermore Apricoxib had lowest IC50 value against MCF-7 cell line. Conclusion: This study demonstrated that beside of primarily anti-inflammatory effects Apricoxib have additional benefits in term of exerting the cytotoxic effects (in vitro) on cancerous cell lines as indicated by reducing the % viability and reducing the Absorbance value of test sample as compare to control. This opens the newer path for researcher to evaluate different aspects of Apricoxib in field of chemotherapy.

scholarly journals Cytotoxic activities of Methanolic and Chloroform Extract of Cryptocarya Massoy (Oken) Kosterm. Bark on MCF-7 human breast cancer cell line

2018 ◽  
Vol 9 (1) ◽  
pp. 57-62
Author(s):  
Yuli Widiyastuti ◽  
Ika Yanti M. Sholikhah ◽  
Sari Haryanti
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Mtt Assay ◽  
Cancer Cell Line ◽  
Chloroform Extract ◽  
Cytotoxic Activities ◽  
Litsea Cubeba ◽  
Mcf 7

Abstrak Latar Belakang. Krangean [Litsea cubeba (Lour.) Pers.] Adalah salah satu tanaman aromatik purba di Indonesia. Tanaman ini adalah anggota keluarga Lauraceae, tumbuh liar di dataran tinggi Sumatera, Kalimantan, dan pulau Jawa. Aktivitas antikanker tanaman ini belum banyak dieksplorasi. Penelitian ini bertujuan untuk mengetahui kandungan fitokimia dan aktivitas sitotoksik ekstrak buah krangean pada sel kanker manusia secara in vitro. Metode. Kloroform dan metanol digunakan untuk mempererat bubuk buah kering selama 3x24 jam. Senyawa fitokimia utama ditandai dengan KLT (kromatografi lapis tipis). Uji MTT dilakukan untuk mengamati morfologi dan viabilitas kanker serviks HeLa, kanker payudara MCF-7, dan sel HEPG2 hepar. Hasil. Hasil penelitian menunjukkan bahwa karakterisasi KLT ekstrak kloroform dan metanol Litsea cubeba menunjukkan profil yang sama, dengan senyawa utama yang ditemukan adalah terpenoid dan alkaloid. Uji MTT menemukan bahwa kedua ekstrak memiliki penghambatan kuat pada sel HeLa. Ekstrak kloroform menunjukkan aktivitas sitotoksik yang lebih kuat dibandingkan dengan metanol, dengan nilai IC50 masing-masing 33,7 dan 64,8 μg / mL. Kesimpulan. Esktrak kloroform dan ekstrak metanol dari Litsea cubeba memiliki aktivitas yang kuat terhadap sel HeLa dan aktivitas sedang terhadap sel HEPG2 dan MCF-7.  Selanjutnya disarankan untuk dilakukan penelitian lebih lanjut untuk mengathui senyawa aktif L. cubeba (Lour.) Pers. yang memiliki aktivitas antikanker potensial. Kata kunci: Litsea cubeba (Lour.) Pers., sitotoksik, HeLa, MCF-7, HebG2, MTT assay   Abstract Background. Krangean [Litsea cubeba (Lour.) Pers.] is one of ancient aromatic plants in Indonesia. This plant is the member of Lauraceae family, growing wild on the highlands of Sumatera, Kalimantan, and Java island. The anticancer activity of this plant haven’t been explored extensively.This research aimed to investigate phytochemical content and cytotoxic activity of krangean fruits extract on human cancer cell line in vitro. Method. Chloroform and methanol were used to macerate dried fruits powder for 3x24 hours. Major phytochemical compounds was characterized by TLC (thin layer chromatography). MTT assay was done to observe morphology and viability of HeLa cervical cancer, MCF-7 breast cancer, and HepG2 hepar cancer cell line. Result. The results showed that TLC characterization of chloroform and methanolic extracts of Litsea cubeba revealed similar profile, with the major compound found are terpenoid and alkaloid. The MTT assay found that both extracts have strong inhibition on HeLa cell line. Chloroform extract exhibited stronger cytotoxic activities compared to methanol, with the IC50 values of 33,7 and 64,8 μg/mL respectively. While, the both extract have moderate cytotoxic activities to HEPG2 and MCF-7 cancer cell line indicated by IC50value more than 100 mg/mL. Conclusion. Chloroform and methanolic extract of Litsea cubeba have a strong activity againts HeLa cancer cell lines and moderate activity to HEPG2 and MCF-7, thought chloroform extract of Litsea cubeba has stronger effect on cancer cell line viability. It is well recommended for further studies to investigate the active compound of L. cubeba (Lour.) Pers. for potential anticancer activity.   Key words: Litsea cubeba (Lour.) Pers., cytotoxic, HeLa, MCF-7, HebG2, MTT assay

scholarly journals Ligand substituent effect on the cytotoxicity activity of two new copper(ii) complexes bearing 8-hydroxyquinoline derivatives: validated by MTT assay and apoptosis in MCF-7 cancer cell line (human breast cancer)

RSC Advances ◽  
2021 ◽  
Vol 11 (24) ◽  
pp. 14362-14373
Author(s):  
Arif Ali ◽  
Somesh Banerjee ◽  
Saima Kamaal ◽  
Mohammad Usman ◽  
Neeladrisingha Das ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Mtt Assay ◽  
Substituent Effect ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Human Breast ◽  
Cytotoxicity Activity ◽  
Mcf 7

The presence of –COOH functionality in a copper(ii) complex leads to higher cytotoxicity than that observed for a complex containing a –CN group.

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