Evaluation of the Ovarian Reserve in Women With Systemic Lupus Erythematosus

Objective: Systemic lupus erythematosus (SLE) is a chronic multisystem autoimmune disorder where the disease activity itself and the medications used for its treatment, may have adverse effects on ovarian function. This study aimed to assess the ovarian reserve (OR) in SLE patients. Materials and methods: The anti-müllerian hormone (AMH) and the antral follicle count (AFC), two markers to evaluate the OR was assessed in 64 SLE patients and compared to normal individuals. Additionally, we assessed whether the disease per se or the pharmacological treatments affect the OR. Results: Patients with SLE displayed alterations in the OR regardless of the presence of alterations of the menstrual cycle. The AFC and AMH were significantly lower in SLE patients with and without menstrual alterations when compared to control individuals (p<0.0001). However, the AFC and AMH levels were significantly correlated (p=0.006) in the SLE patients with menstrual alterations. Except for hydroxychloroquine that was statistically higher in SLE patients with menstrual alterations (p=0.04), the cumulative dose for cyclophosphamide, corticosteroid, and methotrexate was similar in SLE patients regardless of the occurrence of menstrual alterations. Conclusion: The monitoring of AMH and AFC in SLE patients should be used to detect the rapid and irreversible decline of the OR to provide a possibility of pregnancy to the SLE patients.

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Characterization of the thrombin generation profile in systemic lupus erythematosus

Physiology International ◽

10.1556/2060.104.2017.1.5 ◽

2017 ◽

Vol 104 (1) ◽

pp. 35-41 ◽

Cited By ~ 4

Author(s):

A Kern ◽

E Barabás ◽

A Balog ◽

Sz Burcsár ◽

M Kiszelák ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Lupus Erythematosus ◽

Thrombin Generation ◽

Area Under The Curve ◽

Autoimmune Disorder ◽

Systemic Lupus ◽

Time To Peak ◽

Cat Assay ◽

Coagulation Tests ◽

Healthy Control

Systemic lupus erythematosus (SLE) is a multisystemic inflammatory autoimmune disorder. Thrombotic events occur at a higher incidence among SLE patients. The investigation of thrombin generation (TG) with calibrated automated thrombogram (CAT) test as a global hemostasis assay is applicable for the overall functional assessment of the hemostasis. The aim of this study was to characterize the hemostatic alterations observed in SLE by CAT assay. In this study, CAT parameters and basic coagulation parameters of SLE patients (n = 22) and healthy control subjects (n = 34) were compared. CAT area under the curve (i.e., endogenous thrombin potential) was lower than normal in SLE (807 vs. 1,159 nM*min, respectively), whereas other CAT parameters (peak, lag time, time to peak, and velocity index) and the basic coagulation tests were within the normal range. The presence of anti-phospholipid antibodies and the applied therapy was not associated with hemostasis parameters in SLE. We concluded that the reported high risk of thrombosis is not related to TG potential.

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MiR-410 Down-Regulates the Expression of Interleukin-10 by Targeting STAT3 in the Pathogenesis of Systemic Lupus Erythematosus

Cellular Physiology and Biochemistry ◽

10.1159/000445625 ◽

2016 ◽

Vol 39 (1) ◽

pp. 303-315 ◽

Cited By ~ 23

Author(s):

Dongmei Liu ◽

Na Zhang ◽

Xiaomei Zhang ◽

Muting Qin ◽

Youdan Dong ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

T Cells ◽

Lupus Erythematosus ◽

Autoimmune Disorder ◽

Interleukin 10 ◽

Luciferase Assay ◽

Mrna Levels ◽

Healthy Controls ◽

Systemic Lupus ◽

Targeting Effect

Background/Aims: Systemic lupus erythematosus (SLE) is a heterogeneous chronic inflammatory autoimmune disorder, in the pathogenesis of which miRNAs play a versatile function. The purpose of this study was to investigate the effect of miRNA-410 on the pathogenesis of SLE in T cells of SLE patients. Methods: Real-time PCR was used to test the mRNA levels of miRNA-410 in SLE patients and healthy controls. ELISA analysis was performed to examine the production levels of IL-10. Luciferase Assay was used to confirm the targeting effect of miRNA-410 on 3'UTR of STAT3 mRNA. Results: We found that the expression level of miR-410 in T cells of SLE patients was decreased comparing to that in healthy controls, whereas overexpression of miR-410 significantly reduced the expression levels of IL-10. Furthermore, miR-410 suppresses the transcription activity of STAT3 by binding directly to the 3 'UTR of STAT3 mRNA. Moreover, silence of STAT3 down regulated IL-10 expression in CD3+ T cells. Conclusion: Our results demonstrate that miR-410 is the key regulatory factor in the pathogenesis of SLE by regulating the expression of IL-10 through targeting STAT3. These data suggest a novel function of miR-410 and bring new insight into understanding the complex mechanisms involved in SLE.

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A Case of Thrombotic Thrombocytopenia Purpura Associated with Systemic Lupus Erythematosus: Diagnostic Utility of ADAMTS-13 Activity

Autoimmune Diseases ◽

10.4061/2011/483642 ◽

2011 ◽

Vol 2011 ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

Risa Yamada ◽

Kazuhisa Nozawa ◽

Takashi Yoshimine ◽

Yoshinari Takasaki ◽

Hideoki Ogawa ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Lupus Erythematosus ◽

Thrombotic Microangiopathy ◽

Autoimmune Disorder ◽

Accurate Diagnosis ◽

Diagnostic Utility ◽

Systemic Lupus

Thrombotic thrombocytopenia purpura (TTP) caused by a deficiency in ADAMTS-13 activity is considered to involve a subset of thrombotic microangiopathy (TMA). Although concept of TTP is included under the umbrella of TMA, discrimination of TTP from TMA is occasionally difficult in an autoimmune disorder. Herein, we report a case with TTP associated with systemic lupus erythematosus (SLE). In this case, it was difficult to discriminate TTP from TMA and the measurement of ADAMTS-13 activity was useful for obtaining an accurate diagnosis. SLE patients having thrombocytopenia in complication with anemia should be considered a monitoring of ADAMTS-13 activity even though the patients lacked symptoms of TTP related to the microvascular coagulation.

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Correction to: Anti-Müllerian hormone serum levels in systemic lupus erythematosus patients: influence of the disease severity and therapy on the ovarian reserve

Endocrine ◽

10.1007/s12020-018-1811-1 ◽

2018 ◽

Vol 63 (2) ◽

pp. 405-405

Author(s):

Clara Di Mario ◽

Luca Petricca ◽

Maria Rita Gigante ◽

Angelina Barini ◽

Antonella Barini ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Disease Severity ◽

Ovarian Reserve ◽

Lupus Erythematosus ◽

Serum Levels ◽

Systemic Lupus

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Anti-Müllerian hormone serum levels in systemic lupus erythematosus patients: Influence of the disease severity and therapy on the ovarian reserve

Endocrine ◽

10.1007/s12020-018-1783-1 ◽

2018 ◽

Vol 63 (2) ◽

pp. 369-375 ◽

Cited By ~ 6

Author(s):

Clara Di Mario ◽

Luca Petricca ◽

Maria Rita Gigante ◽

Angelina Barini ◽

Antonella Barini ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Disease Severity ◽

Ovarian Reserve ◽

Lupus Erythematosus ◽

Serum Levels ◽

Systemic Lupus

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Expansion of regulatory T cells using low-dose interleukin-2 attenuates hypertension in an experimental model of systemic lupus erythematosus

AJP Renal Physiology ◽

10.1152/ajprenal.00616.2018 ◽

2019 ◽

Vol 317 (5) ◽

pp. F1274-F1284 ◽

Cited By ~ 2

Author(s):

Erin B. Taylor ◽

Jennifer M. Sasser ◽

Kenji J. Maeda ◽

Michael J. Ryan

Keyword(s):

Systemic Lupus Erythematosus ◽

Mean Arterial Pressure ◽

Arterial Pressure ◽

Lupus Erythematosus ◽

Renal Injury ◽

Low Dose ◽

Interleukin 2 ◽

Autoimmune Disorder ◽

Systemic Lupus ◽

And Control

Systemic lupus erythematosus (SLE) is a chronic multisystem autoimmune disorder that is characterized by prevalent hypertension, renal injury, and cardiovascular disease. Numerous studies have reported a low prevalence and/or impaired function of regulatory T (TREG) cells in both patients with SLE and murine models of the disease. Evidence suggests that TREG cell dysfunction in SLE results from a deficiency in IL-2. Recent studies have reported that low-dose IL-2 therapy expands TREG cells in mouse models of SLE, but whether expanding TREG cells protects against hypertension and renal injury during SLE is unclear. To examine this question, female SLE (NZBWF1) and control (NZW) mice were injected with vehicle or recombinant mouse IL-2 three times in 24 h followed by single maintenance doses every 5 days for 4 wk. Treatment with IL-2 effectively expanded TREG cell populations in the peripheral blood, spleen, and kidneys. Circulating levels of anti-dsDNA IgG autoantibodies, a marker of SLE disease activity, were higher in SLE mice compared with control mice but were unaffected by IL-2 treatment. As previously reported by our laboratory, mean arterial pressure, measured in conscious mice by a carotid catheter, was higher in SLE mice than in control mice. Mean arterial pressure was significantly lower in IL-2-treated SLE mice compared with vehicle-treated SLE mice, suggesting that expanding TREG cells using low-dose IL-2 attenuates the development of hypertension. While the mechanism for the protection against hypertension is unclear, it does not appear to be related to the delay of SLE disease progression.

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Systemic lupus erythematosus: A new autoimmune disorder in Kabuki syndrome

European Journal of Medical Genetics ◽

10.1016/j.ejmg.2018.09.005 ◽

2019 ◽

Vol 62 (6) ◽

pp. 103538 ◽

Cited By ~ 2

Author(s):

Todor Arsov ◽

Mario Sestan ◽

Nastasia Cekada ◽

Marijan Frkovic ◽

Dan Andrews ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Lupus Erythematosus ◽

Autoimmune Disorder ◽

Kabuki Syndrome ◽

Systemic Lupus

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Immune Profiling and Precision Medicine in Systemic Lupus Erythematosus

Cells ◽

10.3390/cells8020140 ◽

2019 ◽

Vol 8 (2) ◽

pp. 140 ◽

Cited By ~ 9

Author(s):

Yasuo Nagafuchi ◽

Hirofumi Shoda ◽

Keishi Fujio

Keyword(s):

Systemic Lupus Erythematosus ◽

Precision Medicine ◽

Lupus Erythematosus ◽

Clinical Symptoms ◽

Autoimmune Disorder ◽

Molecular Networks ◽

Systemic Lupus ◽

Disease Heterogeneity ◽

Wide Range ◽

Immune Profiling

Systemic lupus erythematosus (SLE) is an autoimmune disorder with a wide range of clinical symptoms. Enormous progress has been made in the immunological and genetic understanding of SLE. However, the biology of disease heterogeneity in SLE has remained largely unexplored. Human immune profiling studies, helped by recent technological advances especially in single-cell and “omics” analyses, are now shedding light on the cellular and molecular basis of clinical symptoms and disease flares in individual patients. Peripheral blood immunophenotyping analysis with flow cytometry or mass cytometry are identifying responsible cell subsets and markers characteristic of disease heterogeneity. Transcriptome analysis is discovering molecular networks responsible for disease activity, disease subtype and future relapse. In this review, we summarize recent advances in the immune profiling analysis of SLE patients and discuss how they will be used for future precision medicine.

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Role of Cytokines in Systemic Lupus Erythematosus: Recent Progress from GWAS and Sequencing

Journal of Biomedicine and Biotechnology ◽

10.1155/2012/798924 ◽

2012 ◽

Vol 2012 ◽

pp. 1-17 ◽

Cited By ~ 15

Author(s):

John J. Connolly ◽

Hakon Hakonarson

Keyword(s):

Systemic Lupus Erythematosus ◽

Lupus Erythematosus ◽

Association Studies ◽

Monozygotic Twins ◽

Autoimmune Disorder ◽

Genome Wide Association Studies ◽

Systemic Lupus ◽

Genome Wide ◽

Number Variation

Systemic lupus erythematosus (SLE) is a complex autoimmune disorder, known to have a strong genetic component. Concordance between monozygotic twins is approximately 30–40%, which is 8–20 times higher than that of dizygotic twins. In the last decade, genome-wide approaches to understanding SLE have yielded many candidate genes, which are important to understanding the pathophysiology of the disease and potential targets for pharmaceutical intervention. In this paper, we focus on the role of cytokines and examine how genome-wide association studies, copy number variation studies, and next-generation sequencing are being employed to understand the etiology of SLE. Prominent genes identified by these approaches includeBLK, FCγR3B,andTREX1. Our goal is to present a brief overview of genomic approaches to SLE and to introduce some of the key discussion points pertinent to the field.

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