scholarly journals Actividad Inhibitoria De La Hialuronidasa Del Extracto Hidroalcohólico De Piper Peltatum/Hyaluronidase Inhibitor Activity in Hydroalcoholic Extracts of Piper Peltatum

2020 ◽  
Author(s):  
G Pilco ◽  
D Vinueza ◽  
K Acosta ◽  
A Torres
Keyword(s):  
Geographical Area ◽  
Inhibitory Effects ◽  
Naja Naja ◽  
Spectrophotometric Methods ◽  
Antimitotic Activity ◽  
Hyaluronidase Inhibitor ◽  
Bothrops Atrox ◽  
Gallic Acid Equivalent ◽  
Naja Atra

Ecuador se ubica en una zona geográfica privilegiada, presenta zonas climáticas bien definidas con características ambientales y geográficas únicas. Estas particularidades hacen que cada región posea sus propios ecosistemas, existiendo así muchas especies vegetales y animales sin investigar. La población hace uso de estos recursos para tratar diversas dolencias como por ejemplo, Piper peltatum utilizada en caso de mordedura de serpiente. El objetivo del estudio fue determinar si los extractos de esta especie presentaban efectos inhibitorios sobre la hialuronidasa, para lo cual se identificó los metabolitos secundarios presentes mediante el tamizaje fitoquímico, se cuantificó la cantidad de fenoles y flavonoides totales a través de métodos espectrofotométricos y se determinó el porcentaje de inhibición tanto sobre la hialuronidasa bovina como la hialuronidasa presente en el veneno de Naja naja atra y Botrox atrox. Se identificó en P. peltatum alcaloides con potencial actividad antimitótica, cumarinas, terpenos, compuestos fenólicos y flavonoides. La cantidad de fenoles totales fue de 14,49±1,2 mg equivalente de ácido gálico/g extracto seco y 14,53±0,208 mg equivalente de Quercetina/g extracto seco de flavonoides. Respecto a la actividad inhibitoria se concluyó que existe mayor inhibición sobre la hialuronidasa bovina, seguida del veneno de Naja naja atra y no de manera significativa sobre B. atrox. Ecuador is located in a privileged geographical area, it has strongly defined climatic zones with unique environmental and geographical characteristics. These particularities mean that each region has its own ecosystems, so many plant and animal species exist without investigating. The population makes use of these resources to treat various ailments such as Piper peltatum used in case of snakebite. The objective of the study was to determine if extracts of this species has inhibitory effects on hyaluronidase. The secondary metabolites present were identified by phytochemical screening, the amount of phenols and total flavonoids was quantified by spectrophotometric methods, as well as the determination of the inhibition of bovine, Naja naja atra venom and Botrox atrox hyaluronidase. P. peltatum contains alkaloids with potential antimitotic activity, coumarins, terpenes, phenolic compounds and flavonoids. The amount of total phenols was 14.49 ± 1.2 mg gallic acid equivalent / g dry extract and 14.53 ± 0.208 mg Quercetin equivalent /g dry flavonoid extract. Regarding the inhibitory activity, it was concluded that there is greater inhibition on bovine hyaluronidase, followed by Naja naja atra venom and not significantly on B. atrox. Palabras claves: Piper peltatum, hialuronidasa, Naja naja atra, Extracto, Bothrops atrox. Keywords: Piper peltatum, hialuronidase, Naja naja atra, extract, Bothrops atrox.

A single-label fluorescent derivatization method for quantitative determination of neurotoxin in vivo by capillary electrophoresis coupled with laser-induced fluorescence detection

The Analyst ◽  
2016 ◽  
Vol 141 (14) ◽  
pp. 4495-4501 ◽  
Author(s):  
Cuiwei Chen ◽  
Yiqin Hu ◽  
Xiaowei Shi ◽  
Chenghao Tao ◽  
Hongyue Zheng ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Quantitative Determination ◽  
Fluorescence Detection ◽  
Laser Induced Fluorescence ◽  
Short Chain ◽  
Naja Naja ◽  
Laser Induced Fluorescence Detection ◽  
Naja Atra

Neurotoxin (NT), a short-chain α-neurotoxin, is the main neurotoxic protein identified from the venom of Naja naja atra.

Colorimetric Determination of Amoxicillin in Pure and some Pharmaceutical Formulations Via Reaction with Potassium Permanganate as Oxidant Reagent

2019 ◽  
Vol 10 (02) ◽  
Author(s):  
Abbas Shebeeb Al-kadumi ◽  
Sahar Rihan Fadhel ◽  
Mohammed Abdullah Ahmed ◽  
Luma Amer Musa
Keyword(s):  
Potassium Permanganate ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Atomic Emission ◽  
Basic Medium ◽  
Photometric Method ◽  
Colorimetric Determination ◽  
Spectrophotometric Methods ◽  
Label Claim

We proposed two simple, rapid, and convenient spectrophotometric methods are described for the determination of Amoxicillin in bulk and its pharmaceutical preparations. They are based on the measurement of the flame atomic emission of potassium ion (in first method) and colorimetric determination of the green colored solution for manganite ion at 610 nm formed after reaction of Amoxicillin with potassium permanganate as oxidant agent (in the second method) in basic medium. The working conditions of the methods were investigated and optimized. Beer's law plot showed a good correlation in the concentration range of 5-45 μg/ml. The detection limits and relative standared deviations were (2.573, 2.814 μg/ml) (2.137, 2.498) for the flame emission photometric method and (1.844, 2.016 μg/ml) (1.645,1.932) for colorimetric methods for capsules and suspensions respectively. The methods were successfully applied to the determination of Amoxicillin in capsules and suspensions, and the obtained results were in good agreement with the label claim. No interference was observed from the commonly encountered additives and expectancies.

Eco-friendly spectrophotometric methods for assessment of alfuzosin and solifenacin in their new pharmaceutical formulation; Green profile evaluation via eco-scale and GAPI tools

2020 ◽  
Vol 16 ◽  
Author(s):  
Mamdouh R. Rezk ◽  
Mina Wadie ◽  
Soheir A. Weshahy ◽  
Mahmoud A. Tantawy
Keyword(s):  
Minor Component ◽  
Time Saving ◽  
Prostate Hyperplasia ◽  
Spectrophotometric Methods ◽  
Ratio Difference ◽  
Ich Guidelines ◽  
Mean Centering ◽  
Benign Prostate ◽  
Urological Diseases

Background: Alfuzosin is recently co-formulated with solifenacin for relieving two coincident urological diseases, namely; benign prostate hyperplasia and overactive bladder Objective: Herein, green, simple and rapid spectrophotometric methods were firstly developed for simultaneous determination of the two cited drugs in their co-formulated pharmaceutical capsule Methods: Alfuzosin, which is the major component in the dosage form, was directly assayed at its extended wavelength at 330.0 nm. The challenging spectrum of the minor component, solifenacin, was resolved by five spectrophotometric methods, namely; dual wavelength (DW) at 210.0 & 230.0 nm, first derivative (1D) at 222.0 nm, ratio difference (RD) at 217.0 - 271.0 nm , derivative ratio (1DD) at 223.0 and mean centering of ratio spectra (MC) at 217.0 nm Results: The Proposed methods were successfully validated as per ICH guidelines. Alfuzosin showed linearity over the range of 4.0 - 70.0 μg/mL, while that of solifenacin were 4.0 - 50.0 μg/mL for DW, 2.0 - 70.0 μg/mL for 1D and RD methods, 1.0 - 70.0 μg/mL for 1DD and 4.0 - 70.0 μg/mL for MC method. Statistical comparison with their official ones showed no noticeable differences. The methods showed good applicability for assaying drugs in their newly combination. Besides eco-scale, the greenness profile of the methods was assessed and compared with the reported spectrophotometric one via the newest metric tool; green analytical procedure index (GAPI). Conclusions: The proposed methods are superior in not only being smart, accurate, selective, robust and time-saving, but also in using distilled water as an eco-friendly and cheap solvent

scholarly journals Cardiotoxin II from Taiwan cobra venom, Naja naja atra. Structure in solution and comparison among homologous cardiotoxins.

1994 ◽  
Vol 269 (38) ◽  
pp. 23500-23508
Author(s):  
R. Bhaskaran ◽  
C.C. Huang ◽  
Y.C. Tsai ◽  
G. Jayaraman ◽  
D.K. Chang ◽  
...  
Keyword(s):  
Cobra Venom ◽  
Naja Naja ◽  
Structure In Solution ◽  
Naja Atra ◽  
Cardiotoxin Ii

scholarly journals Simultaneous Estimation of Domperidone and Pantoprazole in Solid Dosage Form by UV Spectrophotometry

2006 ◽  
Vol 3 (3) ◽  
pp. 142-145
Author(s):  
P. Ravi Kumar ◽  
P. Bhanu Prakash ◽  
M. Murali Krishna ◽  
M. Santha Yadav ◽  
C. Asha Deepthi
Keyword(s):  
Simultaneous Equation ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Uv Spectrophotometry ◽  
Q Value ◽  
Value Analysis ◽  
Spectrophotometric Methods ◽  
Solid Dosage ◽  
Tablet Dosage

Domperidone is an antiemetic and pantoprazole is an antiulcer drug. Simple, precise, rapid and selective simultaneous equation and Q- analysis UV spectrophotometric methods have been developed for the simultaneous determination of domperidone and pantoprazole from combined tablet dosage forms. The methods involve solving of simultaneous equations and Q-value analysis based on measurement absorptivity at 216, 287 and 290 nm respectively. Linearity lies between 1-15 mcg/mL for domperidone and 0-50 mcg/mL for pantoprazole.

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