scholarly journals Sources of Arterial Vascularization of the Polar Owl’s Kidneys

2021 ◽  
Author(s):  
Marina V. Pervenetskaya ◽  
Lydmila V. Fomenko ◽  
Maksim V. Koshkarev
Keyword(s):  
Connective Tissue ◽  
Collagen Fibers ◽  
Renal Arteries ◽  
Muscle Membrane ◽  
Elastic Fibers ◽  
Elastic Membrane ◽  
High Concentration ◽  
Loose Connective Tissue ◽  
Internal Iliac ◽  
Oil Paints

The sources of vascularization of the kidneys of five polar owl carcasses were studied by filling the vessels with self-hardening plastic Belokril through the femoral artery. High-grade oil paints were added to the monomer to give the vessels the desired color. After the injection, the carcasses were placed in a high concentration caustic soda solution for three days. The resulting corrosion impression was washed under warm water and dried. It was identified that in the lumbar trunk, the main vessel was the descending aorta, from which extra- and intraorganic arteries departed for vascularizing the kidneys. Extraorganic arteries included external and internal iliac, sciatic and middle sacral arteries. Intraorganic arteries included cranial, middle, and caudal renal arteries. Inside the parenchyma of each lobe of the kidney, intraorganic arteries branched in the main type of caudomedial, dorsomedial and lateromedial directions and were subdivided into segmental, interlobular and perilobular arteries and intralobular capillaries. An asymmetry in the branching of the renal arteries was observed. During histological examination, we noted that the renal arteries were lined with endothelium on the inner side and the intima contained endotheliocytes with oval nuclei. Under the endothelial layer were loose collagen fibers running along the middle shell. There was no loose connective tissue between the inner and middle shells, so the subendothelial layer was very weak and there was no internal elastic membrane. The muscle membrane was well developed, with collagen and elastic fibers located between the muscle fibers. The outer shell was represented by loose connective tissue with the presence of arterial and venous vessels. The collagen fibers had a slightly convoluted course. Keywords: birds, polar owl, arteries, kidneys, parenchyma, capillaries, endotheliocytes, intima

scholarly journals ULTRASTRUCTURAL STUDIES ON THE LYMPHATIC ANCHORING FILAMENTS

1968 ◽  
Vol 36 (1) ◽  
pp. 129-149 ◽  
Author(s):  
L. V. Leak ◽  
J. F. Burke
Keyword(s):  
Connective Tissue ◽  
Extracellular Space ◽  
Collagen Fibers ◽  
Capillary Wall ◽  
Surrounding Tissue ◽  
Elastic Fibers ◽  
Lymphatic Capillary ◽  
Ultrastructural Studies ◽  
Outer Leaflet ◽  
Tissue Area

The fine structure of the lymphatic capillary and the surrounding tissue areas was investigated. Instead of a continuous basal lamina (basement membrane) surrounding the capillary wall, these observations revealed the occurrence of numerous fine filaments that insert on the outer leaflet of the trilaminar unit membrane of the lymphatic endothelium. These filaments appear as individual units, or they are aggregated into bundles that are disposed parallel to the long axis of the lymphatic capillary wall and extend for long distances into the adjoining connective tissue area among the collagen fibers and connective tissue cells. The filaments measure about 100 A in width and have a hollow profile. They exhibit an irregular beaded pattern along their long axis and are densely stained with uranyl and lead. These filaments are similar to the microfibrils of the extracellular space and the filaments observed in the peripheral mantle of the elastic fibers. Infrequently, connections between these various elements are observed, suggesting that the lymphatic anchoring filaments may also contribute to the filamentous units of the extracellular space. It is suggested that these lymphatic anchoring filaments connect the small lymphatics to the surrounding tissues and represent the binding mechansim that is responsible for maintaining the firm attachment of the lymphatic capillary wall to the adjoining collagen fibers and cells of the connective tissue area.

scholarly journals Teeth and their Associated Tissues

2000 ◽  
Vol 8 (1) ◽  
pp. 18-21 ◽  
Author(s):  
Barry R. J. Rittman
Keyword(s):  
Connective Tissue ◽  
Soft Tissues ◽  
Collagen Fibers ◽  
Loose Connective Tissue ◽  
Histological Sections ◽  
Surrounding Bone

The preparation of histological sections of teeth can be both a rewarding and a frustrating experience. This is primarily due to the varying degrees of mineralization of the enamel, dentin, cementum and surrounding bone and the difficulty in retaining original relationships between the calcified and the soft tissues in the final stained section.The tooth has a central pulp that is a gelatinous loose connective tissue. Surrounding this is dentin that forms the main bulk of the tooth, is composed chiefly of collagen fibers and is approximately 70% mineralized.

scholarly journals THE BEHAVIOR OF ELASTIC TISSUE IN THE POSTFETAL OCCLUSION AND OBLITERATION OF THE DUCTUS ARTERIOSUS (BOTALLI) IN SUS SCROFA

1914 ◽  
Vol 19 (2) ◽  
pp. 129-142 ◽  
Author(s):  
J. Parsons Schaeffer
Keyword(s):  
Connective Tissue ◽  
Sus Scrofa ◽  
Ductus Arteriosus ◽  
Elastic Fibers ◽  
Elastic Membrane ◽  
Tissue Cell ◽  
Elastic Tissue ◽  
Tissue Cells ◽  
Ductus Arteriosus Botalli ◽  
Elastic Fibrils

A study of the histogenesis of elastic tissue in the embryonic ductus arteriosus of Sus scrofa is in accord with the theory that elastic fibrils are directly differentiated in the outlying portion of the protoplasm of the early connective tissue cell. In the occlusion of the postfetal ductus arteriosus of Sus scrofa there is early a hypertrophy of the internal elastic membrane. Subsequently there takes place a marked delamination of the thickened internal elastic membrane in the production of new and independent elastic fibers and lamellæ. The formation of new elastic fibers from preformed elastic tissue is most abundant where the postfetal contraction of the ductus arteriosus is least marked. These new elastic fibers play an important part in the occlusion of the lumen of the postfetal ductus. Aside from the extensive formation of elastic fibers from preformed elastic tissue, in the occlusion of the lumen of the postfetal ductus arteriosus of Sus scrofa, there are also some elastic fibrils formed from non-elastic elements, apparently from connective tissue cells. In some recent preliminary work on ligations of the common carotid artery there was found, after an interval of from eight to twelve days, at some points between the ligatures, a slight but obvious cellular thickening of the so-called subendothelial stratum. Some of these connective tissue cells may have wandered from the other coats of the vessel, through the inner elastic membrane into the subendothelial stratum; others may have proliferated from cells in situ. Specific stains revealed near the periphery of some of these cells, i. e., in the outlying portion of the exoplasm, very delicate elastic fibrils, apparently the product of protoplasmic activity.

scholarly journals Ultrastructure of Granulosa Cells of Bovine Ovarian Antral Follicles in Relation to Atresia

2020 ◽  
Vol 64 (3) ◽  
pp. 22-30
Author(s):  
J. Pivko ◽  
L. Olexiková ◽  
L. Dujíčková ◽  
A. V. Makarevich
Keyword(s):  
Connective Tissue ◽  
Granulosa Cells ◽  
Smooth Endoplasmic Reticulum ◽  
Ovarian Follicle ◽  
Basal Membrane ◽  
Collagen Fibers ◽  
Cell Organelles ◽  
Loose Connective Tissue ◽  
Blood Capillaries ◽  
Antral Follicles

AbstractThe aim of this study was to describe the most common forms of ovarian follicle atresia in large antral follicles of cows and quantify the occurrence of different cell structures in each form. Atresia of antral follicles in ovaries was determined on the basis of ultrastructural images acquired by electron microscopy of ultrathin sections contrasted with uranyl acetate and lead citrate to visualize cell organelles. All forms of atresia in large follicles are accompanied by regressive changes of the granulosa cells. The initial form of atresia is characterized by enlarged intercellular spaces after the disruption of the gap junctions and desmosomes. Small collapsed cells with pyknotic nuclei, substantially reduced the cytoplasm and a higher incidence of lysosomes are located on the surface of the granulosa layer. The stratum granulosum wall collapses and the basal membrane is swollen with a rupture of the lamina basalis. Obliterative atresia is characterized by a multiplied loose connective tissue consisting of collagen fibers, fibroblasts, histiocytes, blood capillaries and sporadically granulocytes. The cystic form of atresia is characterized by small collapsed, pyknotic granulosa cells settled in one or two layers. In luteinization-associated atresia, granulosa cells are hypertrophied, their cytoplasm contains smooth endoplasmic reticulum and mitochondria with tubules. In conclusion, the initial atresia of large antral ovarian follicles is associated with processes of cell death, followed by multiplication of the loose connective tissue cells, its dystrophy and hyalinization of the collagen fibers. Ultrastructural examination could be used as a complementary method to improve histopathological diagnostics of cow reproductive organs in veterinary practice.

scholarly journals Morphology of the esophageal mucosa in long-eared owl and eurasian jay

2018 ◽  
Vol 20 (83) ◽  
pp. 200-203
Author(s):  
N. Dyshlyuk
Keyword(s):  
Connective Tissue ◽  
Lamina Propria ◽  
Elastic Fibers ◽  
Esophageal Mucosa ◽  
Muscularis Mucosae ◽  
Loose Connective Tissue ◽  
Smooth Muscle Tissue ◽  
Tissue Samples ◽  
Paraffin Block ◽  
Mucosal Folds

The morphology of the esophageal mucosa in long-eared owl (Asio otus) and Eurasian jay (Garrulus glandarius) has been studied. Samples with exception of the region of the esophageal tonsil were obtained from 3 adult birds of each species. They were studied by light microscopy using classical methods of morphological research. The tissue samples were fixed in 10% neutral buffered formalin and embedded in paraffin block and finally the section were cut at 5–10 µm thickness using sliding microtome and stained with hematoxylin and eosin, according to Van Gizon and Weigert and impregnated with silver nitrate according to Kelemen. The esophageal mucosa consisted of four layers: epithelium, lamina propria, muscularis mucosae, and submucosa. The epithelium was stratified squamous and better developed in the cranial part of the esophagus. The lamina propria was represented by a loose connective tissue with small amount of elastic fibers. In the long-eared owl tightly located numerous small secretory sections of the esophagus glands organised in 1–3, and in branches of large folds up to 6 layers, were found. Their short excretory ducts opened to the surface of the mucosa. The muscularis mucosae was represented by a smooth muscle tissue with bundles of cells directed longitudinally and in some areas appeared to be absent. The submucosa was well developed, and as well as lamina propria was formed by a loose connective tissue with a large number of elastic fibers. In the submucosa of the Eurasian jay secretory departments of large, not tightly located, esophageal mucous glands were found. The esophageal mucosa of this birds formed well-developed folds, which were composed of  all its layers. Their number in the long-eared owl was 20–25 and in the Eurasian jay – 5–9. The shape of folds on the transverse section is not the same: in the long-eared owl it was finger-shaped and leaf-shaped, but in the Eurasian jay it was wedge-shaped. Some large folds in the long-eared owl branched into the secondary mucosal folds. The height of folds in cranial part of the esophagus was larger than in caudal part. In both parts of the esophagus of long-eared owl  the height of the large and middle folds was larger than that in the Eurasian jay. The height of small folds in the Eurasian jay was greater than that in the long-eared owl. In both parts of the esophagus of this birds, the fold’s width at the base exceeded its width at the top. In the Eurasian jay the width of base and top of large, medium and small folds was larger than that in the long-eared owl. The esophageal mucosa was poor on immune formations. They were represented by single accumulations of diffuse lymphoid tissue, and in the Eurasian jay – even by secondary lymphoid nodules.

Zum histochemischen und färberischen Verhalten des Koriums bei der Solardermatitis des Rindes

1968 ◽  
Vol 5 (4) ◽  
pp. 297-312
Author(s):  
H.-J. Herrmann
Keyword(s):  
Connective Tissue ◽  
Collagen Fibers ◽  
Elastic Fibers ◽  
Acid Mucopolysaccharides

Early changes in photodynamic dermatitis of a 12-year-old black-spotted cow (Holstein cross) were characterized by splitting of collagen fibers and loss of acid mucopolysaccharides which were believed to be the result of a process of disaggregation and depolymerization. The resorcin- and fuchsinophilic material in the chronically inflammed corium was shown histochemically to be a mucoprotein similar to the cement substance in pathologically altered elastic fibers. Necrosis of connective tissue and a defective but increased synthesis of fibers were considered responsible for the production of this material. Finally, the comparative pathological aspects of this “elastotic” degeneration of connective tissue were discussed.

scholarly journals Histochemical picture in the skin of rats 1, 3, 7, 14, 21 and 30 days after burning of ІІ-ІІІ degrees on the background of injection during first 7 days of 0.9% NaCl solution

2018 ◽  
Vol 24 (2) ◽  
pp. 50-56
Author(s):  
О.Ye. Маievskyi ◽  
К.S. Volkov ◽  
Z.M. Nebesna ◽  
Ye.V. Mironov ◽  
A.V. Shayuk
Keyword(s):  
Connective Tissue ◽  
Collagen Fibers ◽  
The Body ◽  
Elastic Fibers ◽  
Nacl Solution ◽  
Intercellular Substance ◽  
Amorphous Substance ◽  
Thermal Trauma ◽  
Early Stages ◽  
Acidic Glycosaminoglycans

For pathogenetically grounded therapy of burn disease, aimed at reducing the severity of the pathological process, the rapid recovery of microcirculation and stimulation of reparative processes, it is necessary to study the fine mechanisms of reparative regeneration in the skin on the microscopic and biochemical levels. The purpose of the study is to investigate the state of elastic and collagen fibers, as well as glycoproteins and glycosaminoglycans in the intercellular substance of the dermis of rats 1, 3, 7, 14, 21 and 30 days after the burning of the II-III degree, with the background of the injection during first 7 days of 0.9% NaCl solution. An experimental study of structural changes in the skin after burn injury was performed on laboratory white rats, males weighing 150-160 g. Rats were divided into 2 groups, which previously, under the conditions of propofol anesthesia 60 mg/kg internally, catheterization of the femoral vein and depilation lateral surfaces of the trunk of rats. Group 1 - animals without thermal trauma of the skin, which once a day for the first 7 days were injected intravenous infusion of 0.9% NaCl solution in a dose of 10 ml per kg. Group 2 - animals who were also injected once a day with the first 7 days of infusion of 0.9% NaCl solution in a dose of 10 ml per kg after 2-3 degree skin burns, with a total area of 21-23% of the body surface of the rat. Euthanasia of rats was performed after propofol anesthesia (60 mg/kg i/v) by decapitation. Changes in the histochemical structure of bits of skin from the edge of the wound were studied after 1, 3, 7, 14, 21, and 30 days from the beginning of the experiment. Histological sections 5-6 microns thick were stained with Weigert’s elastic stain and dyed with picric acid by Van Gieson’s method (for the detection of elastic and collagen fibers); the PAS + Hale reaction was performed by the Moury method (detection of glycoproteins and glycosaminoglycans in the intercellular substance of the dermis). The histochemical investigations of the area of the II-III degree of skin damaged by burns on the background of the injection of 0.9% NaCl solution found that the degree of change depends on the duration of the experiment. In the early stages of the experiment, the reorganization of the intercellular substance of the dermis manifests itself as damage to the fibrous structures of its papillary and reticular layers. Histochemically, the components of amorphous connective tissue substance are dominated by glycoproteins, the content of acidic glycosaminoglycans is small. In the late stages of the experiment (after 14, 21 and 30 days) in the connective tissue of the affected skin in the central and marginal regions poorly structured collagen and elastic fibers are observed. The pronounced “Hale”-positive coloration reflects an increase in the content of sulfated glycosaminoglycans in the amorphous substance of the connective tissue. Thus, the experimental thermal trauma of skin II-III degree on the background of the introduction during the first 7 days, 0.9% of the NaCl solution is histochemically characterized by reorganization of the intercellular substance of the connective tissue of the dermis. In the early stages of the experiment there is damage to the fibrous structures of the papillary and reticular layers, the prevalence of glycoproteins and the insignificant content of acidic glycosaminoglycans in the amorphous substance, indicating a significant inhibition of adaptive-compensatory processes. In the long term, after the thermal defeat in the dermis, histochemically, an increase in the amount of acid glycosaminoglycans is detected and the PAS positivity of the collagen fibers of the intercellular substance is well expressed. This indicates a slow updating of the amorphous substance and fibrous structures of the connective tissue of the dermis, a violation of regenerative processes.

scholarly journals ВПЛИВ ТИПОЛОГІЧНИХ ОСОБЛИВОСТЕЙ АВТОНОМНОГО ТОНУСУ НА КІЛЬКІСНІ ПОКАЗНИКИ СПОЛУЧНОЇ ТКАНИНИ КИШКОВОЇ СТІНКИ КУРЕЙ

2016 ◽  
Vol 18 (2(66)) ◽  
pp. 180-185
Author(s):  
A.M. Tybinka
Keyword(s):  
Connective Tissue ◽  
Intestinal Wall ◽  
Muscle Membrane ◽  
Elastic Fibers ◽  
Functional Stability ◽  
Paraffin Sections ◽  
Different Types ◽  
Respective Type ◽  
Computer Morphometry ◽  
The Relationship

Fibrous connective tissue of the intestinal wall supports its functional stability and morphological integrity. Therefore, the aim of this research was to investigate the relationship between the fibrous components of connective tissue in the intestinal wall of chicken, which is characterized by different types of autonomous tone.The researches was carries out on laying chicken of Isa-Braun cross, in amount 33 animals, aged 1 year. According to the results of electrocardiographic and variation-pulse metric researches, poultry was divided into two groups: sympatotonics (CT) – 16 chickens and sympato-normotonics (CT-HT) – 17 chickens. After poultry slaughter, from each intestine was extracted wall samples, which were fixed in fluid Buena with the further making of paraffin sections. The coloring of histological sections was done, using the methods of Van Gieson's stain, Pachini and Weigert. The correlation between the size of all fibers of connecting tissue and the size of only elastic fibers in both groups of poultry was researched on ready histological preparations. These researches were carried out on the section of crypt of mucosa and muscle membrane by the way of using computer morphometry. The results of the research were worked out statistically.It was found, on the basis of the obtained results, that the researched correlation is characterized by the same dynamics in the both intestine membranes. Herewith along the whole intestine larger numbers of this correlation belong to chicken-CT. The part of elastic fibers in muscle membrane is higher than in mucosa. The maximum amount of elastic fibers in the content of all fibers of connecting tissue can be observed at the beginning of small intestine and along with it gradually reduces. At the beginning of the large intestine this part slightly increase, however in the end of the intestine has the smallest amount. The presented patterns is peculiar for the both types of poultry, so on the basis of this we can mention the relative stability of morphology of intestine connective tissue. And disclosed typological peculiarities of the autonomous tone allow to adapt the structure of fiber component of connective tissue to the functional characteristics of digestion during the respective type of autonomous balance.

Sign in / Sign up

Export Citation Format

Share Document