Background & Aims:
Hepatocellular carcinoma (HCC) is a highly aggressive cancer
with few treatment options. Toll-like receptor 3 (TLR3) plays a key role in innate immunity and may
affect the development of cancers. This study aimed to investigate the association between TLR3
gene polymorphism and HCV-related hepatocellular carcinoma in Egypt.
Methods:
This work was conducted on 70 individuals; fifty HCV cirrhotic patients were included in
two groups; with HCC (30 patients) and without HCC (20 patients) compared with a group of 20
apparently healthy controls. All of the studied individuals underwent clinical-laboratory evaluation.
TLR3 gene single-nucleotide polymorphism (SNP) (+1234C/T) was tested by polymerase chain reaction-
restriction fragment length polymorphism.
Results:
This study reported that the prevalence of TLR3 +1234TT genotype was significantly increased
in cirrhotic patients with HCC than without HCC, while it was not detected at all among the
controls. When analyzing the TLR3 SNP +1234C/T with different clinical parameters in HCC patients,
there was a significant association between+1234C/T SNP; namely TT genotype and each of
the hepatic focal lesions᾽ number, size and the patients᾽ higher Okuda and BCLC stages. No association
could be detected between TLR3 SNP and the age, sex, Child-Pugh grades, MELD score or
AFP of the studied HCC cases.
Conclusion:
TLR3 gene SN P +1234C/T could be a novel risk factor for the HCV-related HCC
among the Egyptian population.
Proteomic analysis of laser capture microdissected focal lesions in a rat model of progenitor marker-positive hepatocellular carcinoma
