Association of haplotypes for SNPs in the LTR regions of bovine leukemia virus with hematological indices of cattle

Molecular typing of BLV samples isolated from Holsteinized Russian Black Pied cattle was carried out, and various cytofluorometric and morphological blood indices were examined. We performed the total count of white blood cells (WBC), lymphocyte (lymf), granulocyte (gran), monocyte (mon), red blood cell (RBC), hemoglobin (HGB), hematocrit (HTC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red blood cell distribution width (RDW), platelet count (PLT), mean platelet volume (MPV), platelet distribution width (PDW), and platelet crit count (PCT). The LTR-region of BLV was haplotyped. Only viruses of haplotypes I (0.33±0.03) and III (0.67±0.03) of the eight possible were detected. The ratio of hematologically sick, healthy, and suspected carriers of BLV of haplotypes I and II was comparable with the results of other researchers. The numbers of leukocytes, erythrocytes and platelets in the blood of carriers of haplotype III exceeded the corresponding parameters of cattle affected by the virus of haplotype I. It is interesting to note that the difference in the hemolytic status of animals was manifested not only by the concentration of leukocytes as direct immune agents but also by the count of erythrocytes and platelets, which are not directly involved in the immune response. The number of particles of haplotype III of the BLV circulating in the blood of infected individuals exceeded that of the carriers of haplotype I. In this connection, an assumption was made about the evolutionary advantage of the more virulent haplotype III. However, the results of our own research in conjunction with the data of other scientists indicate that the high virulence of individual virus strains is a consequence of the tendency to implement the maximum possible intensity of the synthesis of virus particles but not of the high damaging effect alone. It is shown that high lethality is evolutionarily disadvantageous for viruses, since the extinction of the carrier as a biological species is fraught with the disappearance of the virus itself.

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Comparative effect of probiotic (Saccharomyces cerevisiae), prebiotic (Fructooligosaccharide) and their combination on some blood indices in young common carp (Cyprinus carpio L.)

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v40i1.131 ◽

2016 ◽

Vol 40 (1) ◽

pp. 9-15 ◽

Cited By ~ 1

Author(s):

Nasreen M. Abdulrahman

Keyword(s):

Saccharomyces Cerevisiae ◽

Blood Cell ◽

Common Carp ◽

Red Blood Cell ◽

Blood Parameters ◽

Average Weight ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Hemoglobin ◽

Yeast Saccharomyces Cerevisiae ◽

Blood Indices

This study was carried out to examine the effect of Fructooligosaccharide as a source of prebiotic, and commercial dry yeast (Saccharomyces cerevisiae) as a source of probiotic and their combination in different level as a source of synbiotic. The experiment was conducted in the fish laboratory of Animal Production Department, Faculty of Agricultural Sciences of Sulaimani University. The trials lasted for three months after 21 days of adapting period 120 common carp fingerlings with an average weight of 20 ±2 gm, were used to test the effect of different levels of the Fructooligosaccharide, yeast and their combination. In T1 fish were fed a diet with 2.5 gm/kg Fructooligosaccharide, in T2, fish were fed a diet 2.5 gm/kg yeast, T3 represents the third treatment, in which fish were fed on a diet 5 gm/kg Fructooligosaccharide. While, in T4 fish were fed a diet 5 gm/kg yeast, and T5 2.5 Fructooligosaccharide: 2.5 Yeast (gm/kg), T6 was 5 Fructooligosaccharide: 5 yeast (gm/kg), T7 2.5 Fructooligosaccharide: 5 Yeast gm/kg), while T8 5 Fructooligosaccharide, 2.5 (gm/kg) Yeast. Each treatment in three replicates in which five fingerlings common carp were stocked in plastic tanks, which fed the experimental diets twice daily. Blood parameters of tested fish showed significant differences in Red blood cell count (1012 cells/l) in T5 and T7 by 1.235 and 1.260, respectively. Hemoglobin (g/dl) data were 117.000 in the fifth treatment. The mean corpuscular hemoglobin (pg/cell) was 121.400 in T2, 137.850 in T3, 121.050 in T4, and 135.300 in T6; mean corpuscular hemoglobin concentration (g/l) was 924.000, in the seventh treatment, mean corpuscular volume were (fL) 232.500 and 233.050 in T4 and T8 respectively. There were different effects of the treatment in the studied blood parameters in which the level of 2.5 g/kg in both Fructooligosaccharide and dry yeast affect significantly the Red blood cell, White blood cell and Hemoglobin.

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Complete Blood Count Peculiarities in Pregnant SARS-CoV-2-Infected Patients at Term: A Cohort Study

Diagnostics ◽

10.3390/diagnostics12010080 ◽

2021 ◽

Vol 12 (1) ◽

pp. 80

Author(s):

Roxana Covali ◽

Demetra Socolov ◽

Razvan Socolov ◽

Ioana Pavaleanu ◽

Alexandru Carauleanu ◽

...

Keyword(s):

Blood Cell ◽

Red Blood Cell ◽

Blood Count ◽

Complete Blood Count ◽

Hemoglobin Concentration ◽

Mean Corpuscular Hemoglobin ◽

Mean Corpuscular Hemoglobin Concentration ◽

Blood Cell Count ◽

Corpuscular Hemoglobin ◽

Distribution Width

Background: During viral outbreaks, pregnancy poses an increased risk of infection for women. Methods: In a prospective study, all patients admitted for delivery at term to Elena Doamna Obstetrics and Gynecology University Hospital in Iasi, Romania, between 1 April 2020 and 31 December 2020 were included. There were 457 patients, divided into two groups: group 1, SARS-CoV-2-positive patients (n = 46) and group 2, SARS-CoV-2-negative patients (n = 411). Among other tests, complete blood count was determined upon admittance, and the following values were studied: white blood cell count, lymphocytes, neutrophils, red blood cell count, hemoglobin, mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, mean corpuscular volume, red blood cell distribution width, hematocrit, platelet count, mean platelet volume, platelet distribution width, plateletcrit, and platelet large cell ratio. Results: in pregnant SARS-CoV-2-infected patients at term, there was a significant decrease in white blood cell, neutrophil, and lymphocyte count, and an increase in mean corpuscular hemoglobin concentration, compared to healthy pregnant women at term, although all still within normal limits. None of the other components of the complete blood count or fetal outcomes studied was significantly influenced by SARS-CoV-2 infection in pregnant patients at term.

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Evaluation of Red Blood Cell Parameters as a Biomarker for Long-Term Glycemic Control Monitoring Among Type 2 Diabetic Patients in Southwest Ethiopia: A Cross-Sectional Study

10.21203/rs.3.rs-504669/v1 ◽

2021 ◽

Author(s):

Misganaw Asmamaw ◽

Tariku Sime ◽

Kumsa Kene ◽

Minale Fekadie ◽

Muluken Teshome ◽

...

Keyword(s):

Blood Cell ◽

Red Blood Cell ◽

Red Cell Distribution Width ◽

Mean Corpuscular Hemoglobin ◽

Red Cell ◽

Cell Distribution ◽

Corpuscular Hemoglobin ◽

Distribution Width ◽

Cell Parameters

Abstract Background: Type 2 Diabetes Mellitus (T2 DM) is a common type of DM characterized by hyperglycemia. Glycation of hemoglobin and related proteins in DM can affect the physiological and structural properties of red blood cells. Although glycated hemoglobin (HbA1c) test continues to be the gold standard for the assessment of long-term glycemic control accessibility and affordability of the test in routine diagnosing service are still limited in developing countries. Hence, this study was aimed to assess red blood cell parameters as a biomarker for long-term glycemic monitoring among T2 DM patients.Methods: Facility-based cross-sectional study through a consecutive sampling technique was conducted among 124 T2 DM patients at the chronic illness follow-up clinic of Jimma Medical Center (JMC) from July 27 to August 31, 2020. A structured questionnaire was used to collect socio-demographic and clinical-related data. Five milliliters of the blood specimen was collected from each eligible T2 DM patient. HbA1c and red blood cell parameters were determined by Cobas 6000 and DxH 800 fully automated analyzers respectively. Data were entered into Epi-data software version 3.1 and exported to SPSS 25 version for analysis. Independent t-test and Pearson’s correlation coefficient were used to address the research questions. A P-value < 0.05 was considered statistically significant. Results: The mean age of study participants was 51.84± 11.6 years. 60.5% of T2 DM patients were in poor glycemic control. There was a significant mean difference between good and poor glycemic controlled T2 DM patients in red blood cell count (4.79±0.5 vs 4.38±0.8), hemoglobin (14.13±1.4 vs 13.60±1.6), mean corpuscular volume (89.52±4.7 vs 92.62±7.5), mean corpuscular hemoglobin (29.63±1.6 vs 30.77±2.9), and red cell distribution width (13.68±1.1 vs 14.63±1.2) respectively. Red blood cell count was inversely correlated (r=-0.280, p=0.002) with HbA1c while mean corpuscular volume (r=0.267, p=0.003), mean corpuscular hemoglobin (r=0.231, p=0.010), and red cell distribution width (r= 0.496, p=0.000) were positively correlated with level of HbA1c. Conclusion: Red cell count, mean corpuscular volume, mean corpuscular hemoglobin, and red cell distribution width could be useful indicators to monitor the glycemic status of T2 DM patients instead of HbA1c, though large prospective studies should be considered.

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Impact of weight loss on inflammation and red blood cell biomarkers after laparoscopic gastric banding surgery

Journal of Investigative Medicine ◽

10.1136/jim-2017-000528 ◽

2017 ◽

Vol 66 (2) ◽

pp. 304-308 ◽

Cited By ~ 5

Author(s):

Susana Coimbra ◽

Cátia Ferreira ◽

Luís Belo ◽

Petronila Rocha-Pereira ◽

Alice Catarino ◽

...

Keyword(s):

Weight Loss ◽

Blood Cell ◽

Gastric Banding ◽

Iron Metabolism ◽

Red Blood Cell ◽

Hemoglobin Concentration ◽

Mean Corpuscular Hemoglobin ◽

Obese Patients ◽

Corpuscular Hemoglobin ◽

Distribution Width

Adipose tissue produces several adipokines that are enrolled in different metabolic and inflammatory pathways that may disturb iron metabolism and erythropoiesis. Considering that laparoscopic adjustable gastric banding (LAGB) has not been associated with a long-term risk of malabsorption, we performed a 13-month follow-up study in severe obese patients submitted to LAGB in order to clarify its impact on inflammation, iron metabolism and on red blood cell (RBC) biomarkers. Twenty obese patients were enrolled in the study, being clinical and analytically assessed before (T0) and 13 months after LAGB intervention (T1). Inflammation, iron bioavailability and RBC biomarkers were evaluated at T0 and T1. At T1, weight and anthropometric indices decreased significantly; patients showed a significant increase in mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration (MCHC), and a reduction in red cell distribution width, ferritin, hepcidin, tumor necrosis factor-α, interleukin-6 (IL-6) and C-reactive protein. Before LAGB, IL-6 correlated negatively with iron, hemoglobin concentration and MCHC; hepcidin correlated inversely with transferrin. Our data show that 13 months after LAGB, the weight loss is associated with an improvement in inflammation, namely a reduction in IL-6 that may reduce hepcidin production, improving iron availability for erythropoiesis, as shown by more adequate erythrocyte hemoglobinization.

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Impacts of Mycoplasma loads and lung lesions on immune and hematological statuses of pigs in an eight-breed cross heterogeneous population

Journal of Animal Science ◽

10.1093/jas/skaa235 ◽

2020 ◽

Vol 98 (8) ◽

Author(s):

Qing Zhang ◽

Jing Li ◽

Tao Huang ◽

Yifeng Zhang ◽

Wenwu Xu ◽

...

Keyword(s):

Blood Cell ◽

Neutrophil Count ◽

Lymphocyte Count ◽

Hemoglobin Concentration ◽

Mean Corpuscular Hemoglobin ◽

Volume Distribution ◽

Mean Corpuscular Hemoglobin Concentration ◽

Corpuscular Hemoglobin ◽

Distribution Width ◽

Lung Lesions

Abstract Developments of pulmonary diseases, often accompanied by infections of bacteria, severely affect the meat production and welfare of pigs. This study investigated 307 pigs at age of 240 d from an eight-breed cross reared under standardized housing conditions for associations among the extent of lung lesions, bacteria load inferred from 16S rRNA sequencing of bronchoalveolar lavage fluid, as well as 57 immune cells and 25 hematological traits. We showed that the pigs under study suffered substantial and varied lung lesions, and the Mycoplasma is the most associated bacteria genera. At a false discovery rate of 0.05 (FDR < 0.05), the severity of lung lesions were significantly associated with greater CD8+ to CD3+ cell ratio, neutrophil-to-lymphocyte ratio (NLR), and standard deviation of red blood cell volume distribution width (RDW-SD), and lower CD4−CD8−/CD3+, CD3+CD4−CD8−/PBMCs (peripheral blood mononuclear cells) and CD14−CD16−/PBMCs cell ratios, mean corpuscular hemoglobin concentration, lymphocyte count, and lymphocyte count percentage, reflecting an status of inflammation, immune suppression, and hypoxia of the pigs accompanying the progression of the lung lesions. The Mycoplasma abundance showed positive correlations with neutrophil count, neutrophil count percentage, NLR, monocyte count, coefficient of variation in red blood cell volume distribution width , and RDW-SD, and negative correlations with mean corpuscular hemoglobin concentration, lymphocyte count, and lymphocyte count percentage; these correlations are largely consistent with those of lung lesions, supporting the comorbidity of lung lesions and Mycoplasma infection. We also observed nonlinear associations that sharp increases in neutrophil count and neutrophil count percentage occurred only when Mycoplasma abundance raised above the population-average level. The results provide helpful insights into the changes of host immune status in response to Mycoplasma relevant lung diseases in pigs.

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Inherited microcytic anemias

Hematology ◽

10.1182/hematology.2020000158 ◽

2020 ◽

Vol 2020 (1) ◽

pp. 465-470

Author(s):

Maria Domenica Cappellini ◽

Roberta Russo ◽

Immacolata Andolfo ◽

Achille Iolascon

Keyword(s):

Iron Deficiency ◽

Blood Cell ◽

Iron Metabolism ◽

Red Blood Cell ◽

Iron Status ◽

Transferrin Saturation ◽

Microcytic Anemia ◽

Deficiency Anemia ◽

Mean Corpuscular Hemoglobin ◽

Distribution Width

Abstract Inherited microcytic anemias can be broadly classified into 3 subgroups: (1) defects in globin chains (hemoglobinopathies or thalassemias), (2) defects in heme synthesis, and (3) defects in iron availability or iron acquisition by the erythroid precursors. These conditions are characterized by a decreased availability of hemoglobin (Hb) components (globins, iron, and heme) that in turn causes a reduced Hb content in red cell precursors with subsequent delayed erythroid differentiation. Iron metabolism alterations remain central to the diagnosis of microcytic anemia, and, in general, the iron status has to be evaluated in cases of microcytosis. Besides the very common microcytic anemia due to acquired iron deficiency, a range of hereditary abnormalities that result in actual or functional iron deficiency are now being recognized. Atransferrinemia, DMT1 deficiency, ferroportin disease, and iron-refractory iron deficiency anemia are hereditary disorders due to iron metabolism abnormalities, some of which are associated with iron overload. Because causes of microcytosis other than iron deficiency should be considered, it is important to evaluate several other red blood cell and iron parameters in patients with a reduced mean corpuscular volume (MCV), including mean corpuscular hemoglobin, red blood cell distribution width, reticulocyte hemoglobin content, serum iron and serum ferritin levels, total iron-binding capacity, transferrin saturation, hemoglobin electrophoresis, and sometimes reticulocyte count. From the epidemiological perspective, hemoglobinopathies/thalassemias are the most common forms of hereditary microcytic anemia, ranging from inconsequential changes in MCV to severe anemia syndromes.

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Infusion of Autologous Retrodifferentiated Stem Cells into Patients with Beta-Thalassemia

The Scientific World JOURNAL ◽

10.1100/tsw.2006.229 ◽

2006 ◽

Vol 6 ◽

pp. 1278-1297 ◽

Cited By ~ 3

Author(s):

Ilham Saleh Abuljadayel ◽

Tasnim Ahsan ◽

Huma Quereshi ◽

Shakil Rizvi ◽

Tamseela Ahmed ◽

...

Keyword(s):

Blood Transfusion ◽

Blood Cell ◽

Red Blood Cell ◽

White Blood Cells ◽

Fetal Hemoglobin ◽

Beta Thalassemia ◽

Mean Corpuscular Hemoglobin ◽

Beta Globin ◽

Globin Chain ◽

Adult Hemoglobin

Beta-thalassemia is a genetic, red blood cell disorder affecting the beta-globin chain of the adult hemoglobin gene. This results in excess accumulation of unpaired alpha-chain gene products leading to reduced red blood cell life span and the development of severe anemia. Current treatment of this disease involves regular blood transfusion and adjunct chelation therapy to lower blood transfusion–induced iron overload. Fetal hemoglobin switching agents have been proposed to treat genetic blood disorders, such as sickle cell anemia and beta-thalassemia, in an effort to compensate for the dysfunctional form of the beta-globin chain in adult hemoglobin. The rationale behind this approach is to pair the excess normal alpha-globin chain with the alternative fetal gamma-chain to promote red blood cell survival and ameliorate the anemia. Reprogramming of differentiation in intact, mature, adult white blood cells in response to inclusion of monoclonal antibody CR3/43 has been described. This form of retrograde development has been termed “retrodifferentiation”, with the ability to re-express a variety of stem cell markers in a heterogeneous population of white blood cells. This form of reprogramming, or reontogeny, to a more pluripotent stem cell state ought to recapitulate early hematopoiesis and facilitate expression of a fetal and/or adult program of hemoglobin synthesis or regeneration on infusion and subsequent redifferentiation. Herein, the outcome of infusion of autologous retrodifferentiated stem cells (RSC) into 21 patients with beta-thalassemia is described. Over 6 months, Infusion of 3-h autologous RSC subjected to hematopoietic-conducive conditions into patients with beta-thalassemia reduced mean blood transfusion requirement, increased mean fetal hemoglobin synthesis, and significantly lowered mean serum ferritin. This was always accompanied by an increase in mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) in such patients. No adverse side effects in response to the infusion of autologous RSC were noted.This novel clinical procedure may profoundly modify the devastating course of many genetic disorders in an autologous setting, thus paving the way to harnessing pluripotency from differentiated cells to regenerate transiently an otherwise genetically degenerate tissue such as thalassemic blood.

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Genome Wide Scan of Complete Blood Count (CBC) Measures Suggests Strong Linkage of Red Blood Cell (RBC) Count to Chromosome 4q25.

Blood ◽

10.1182/blood.v110.11.1729.1729 ◽

2007 ◽

Vol 110 (11) ◽

pp. 1729-1729 ◽

Cited By ~ 1

Author(s):

Diana Abbott ◽

Kai Wang ◽

Amy Shapiro ◽

Trudy Burns ◽

Jorge Di Paola

Keyword(s):

Linkage Analysis ◽

Blood Cell ◽

Complete Analysis ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Hemoglobin ◽

Distribution Width ◽

Large Pedigree ◽

Strong Linkage ◽

Significant Heritability ◽

Von Willebrand

Abstract Previous reports suggest that peripheral blood counts are strongly influenced by environmental and genetic determinants; however few of the genetic factors that regulate these quantitative traits have been discovered. We analyzed CBC data from 395 samples collected from an 854-member Amish pedigree with von Willebrand disease. 71 individuals of the pedigree are heterozygous for a missense mutation at position 4120, represented by a single base substitution (C>T) that predicts an arginine to cysteine change at position 1374 (R1374C) in the A1 domain of the mature von Willebrand factor molecule. The detection of genetic signals is likely to be enhanced in groups that live in a more homogeneous environment like the Amish. Linear and quadratic age effect accounted for varying proportions of the gender-specific variation in the CBC measures (from 1% to 70%). The variance component associated with additive polygenic effects was estimated for each CBC phenotype using MENDEL to obtain estimates of heritability. Significant heritability was found for platelet (h2= 0.518, p <0.0005), white blood cell (WBC) (h2=0.395, p <0.0001), RBC counts (h2= 0.358, p <0.0025) and mean corpuscular hemoglobin concentration (MCHC) (h2= 0.547, p <0.0005). Lower heritability was found for red cell distribution width (RDW) (h2= 0.217, p <0.001) and mean corpuscular hemoglobin (MCH) (h2= 0.231, p < 0.02) hematocrit (Hct) (h2= 0.126, p <0.10) and hemoglobin (Hb) (h2= 0.055, p > 0.2). Interestingly since significant heritability for Hct and Hb was reported in other studies, and to rule out the significant effect of bleeding due to the VWF mutation, we estimated heritability of Hb excluding all members of the pedigree that exhibited the 4120 C>T mutation, h2= 0. 21 (p < 0.05). A primary genetic screen at a 10 cM average interval was performed on the entire Amish pedigree in collaboration with the Marshfield genotyping center. Standard Screening Set 16 with 400 short tandem repeat polymorphic (STRP) markers was utilized generating a total of approximately 160,000 genotypes on the 395 samples. Due to data complexity, the Markov Chain Monte Carlo (MCMC)-based program LOKI was used to conduct multipoint linkage analysis for eight CBC measures that were adjusted for age, sex and mutation status to control for potential confounding. The outcome of interest from the MCMC analysis is represented by the Bayes factor (BF) which examines the probability of linkage in complex pedigrees at every centimorgan (cM). A complete analysis of the genome scan with 1,000,000 iterations was performed. A quantitative trait locus (QTL) suggesting strong linkage was identified for the RBC measure at position 4q25 with a BF of 58.17 at 114.5 cM. This result was confirmed by splitting the pedigree into several smaller subsets and conducting traditional linkage analysis using MERLIN (all individuals in the pedigree were included in at least one subset) with a LOD score of 0.66 (p < 0.05). Interestingly, a previous twin study found evidence for linkage for RBC to 4q32. Similar to animal studies, no evidence of linkage was observed in chromosomal regions known to contain the genes that encode for the hemoglobin chains, erythropoietin or erythropoietin receptor. This analysis in a very large pedigree identified a region of strong linkage for RBC that will be analyzed at higher resolution for the presence of novel modifying genes and alleles that may potentially be important for our understanding of the control of erythropoiesis.

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Variation of Red Blood Cell Distribution Width and Mean Platelet Volume after Moderate Endurance Exercise

Advances in Hematology ◽

10.1155/2014/192173 ◽

2014 ◽

Vol 2014 ◽

pp. 1-4 ◽

Cited By ~ 24

Author(s):

Giuseppe Lippi ◽

Gian Luca Salvagno ◽

Elisa Danese ◽

Cantor Tarperi ◽

Gian Cesare Guidi ◽

...

Keyword(s):

Blood Cell ◽

Endurance Exercise ◽

Red Blood Cell ◽

Mean Platelet Volume ◽

Complete Blood Count ◽

Mean Corpuscular Hemoglobin ◽

Reference Ranges ◽

Platelet Volume ◽

Distribution Width ◽

Haemoglobin Content

Although physical exercise strongly influences several laboratory parameters, data about the hematological changes after medium distance running are scarce. We studied 31 middle-trained athletes (mean training regimen217±32 min/week) who performed a 21.1 km, half-marathon run. Blood samples were collected before the run, at the end, and 3 and 20 hours thereafter. The complete blood count was performed on Advia 2120 and included red blood cell (RBC), reticulocyte, and platelet counts; hemoglobin; mean corpuscular volume (MCV); mean corpuscular hemoglobin (MCH); reticulocyte haemoglobin content (Ret CHR); RBC distribution width (RDW), mean platelet volume (MPV). No significant variations were observed for MCH and Ret CHR. The RBC, reticulocyte, and hemoglobin values modestly decreased after the run. The MCV significantly increased at the end of running but returned to baseline 3 hours thereafter. The RDW constantly increased, reaching a peak 20 hours after the run. The platelet count and MPV both increased after the run and returned to baseline 3 hours thereafter. These results may have implications for definition of reference ranges and antidoping testing, and may also contribute to explaining the relationship between endurance exercise and mortality, since previous studies reported that RDW and MPV may be significantly associated with cardiovascular disease.

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Red Blood Cell Variables in Children and Adolescents regarding the Age and Sex

Iranian Journal of Public Health ◽

10.18502/ijph.v48i4.1004 ◽

2019 ◽

Cited By ~ 1

Author(s):

Jasmina PLUNCEVIC GLIGOROSKA ◽

Serjoza GONTAREV ◽

Beti DEJANOVA ◽

Lidija TODOROVSKA ◽

Daniela SHUKOVA STOJMANOVA ◽

...

Keyword(s):

Blood Cell ◽

Children And Adolescents ◽

Red Blood Cell ◽

Hematological Parameters ◽

Hemoglobin Concentration ◽

Female Group ◽

Distribution Width ◽

Hematological Indices ◽

Significant Difference ◽

Age And Sex

Background: This study aimed to assess the basic red blood cell variables and hematological indices in children and adolescents and analyze the differences regarding age and sex. Methods: Overall, 320 young participants, age 8 to 18 yr, were enrolled at Laboratory of Sport’s Medicine, Medical Faculty, Skopje, Macedonia in 2016. Capillary blood samples were drawn and following hematologic parameters were measured: the red blood cell count (RBC), hemoglobin concentration (Hb), hematocrit level (Hct) and hematological indexes: mean corpuscular volume (MCV), mean hemoglobin concentration (MCH), mean corpuscular hemoglobin concentration (MCHC) and red cell distribution width (RDW). Results: RBC variables in male group showed high statistical level of significance between age different groups (P=0.001) for all studied parameters except MCHC (P=0.423) and RDW (P=0.174). ANOVA test and multivariate tests in female group showed that there was no significant difference for all hematological parameters between age different groups. Regarding the sex differences, male participants had significantly higher red blood count (P<0.001), hemoglobin content (P<0.001) and hematocrit (P<0.001). Conclusion: Hematological parameters in adolescent as inhomogeneous population are not quantified sufficiently, especially hematological indices. RBC variables, regardless of the age, differ very much between male and female examinees, in favor of the male examinees. Hematological indices were insignificantly higher in males. Regarding the age of examinees, RBC variables showed significant inter-groups differences only within male adolescents. While with girls, ages span 8 to 18 yr, we did not find significant differences for most of the hematological variables.

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