Plaque Purification of Bluetongue Virus -12 (BTV-12)

2018 ◽  
Vol 6 (2) ◽  
pp. 128-135
Author(s):  
Srinivas J. ◽  
Keyword(s):  
Bluetongue Virus ◽  
Plaque Purification

scholarly journals Plaque Purification of Bluetongue Virus Serotype-4 (BTV-4)

2018 ◽  
Vol 7 (04) ◽  
pp. 2837-2844
Author(s):  
M. Srikanth Reddy ◽  
Kalyani Putty ◽  
Y. Narasimha Reddy ◽  
P.P. Rao ◽  
M. Ramakoti Reddy ◽  
...  
Keyword(s):  
Bluetongue Virus ◽  
Virus Serotype ◽  
Plaque Purification

Exposure of protein VP7 on the surface of bluetongue virus

1990 ◽  
Vol 48 (3) ◽  
pp. 600-601
Author(s):  
A.D. Hyatt
Keyword(s):  
Bluetongue Virus ◽  
Structural Proteins ◽  
Major Constituent ◽  
Outer Coat ◽  
Virus Particles ◽  
Antibody Recognition ◽  
The Core ◽  
The Family ◽  
Electron Microscopical ◽  
Physical Accessibility

Bluetongue virus (BTV) is the type species os the genus orbivirus in the family Reoviridae. The virus has a fibrillar outer coat containing two major structural proteins VP2 and VP5 which surround an icosahedral core. The core contains two major proteins VP3 and VP7 and three minor proteins VP1, VP4 and VP6. Recent evidence has indicated that the core comprises a neucleoprotein center which is surrounded by two protein layers; VP7, a major constituent of capsomeres comprises the outer and VP3 the inner layer of the core . Antibodies to VP7 are currently used in enzyme-linked immunosorbant assays and immuno-electron microscopical (JEM) tests for the detection of BTV. The tests involve the antibody recognition of VP7 on virus particles. In an attempt to understand how complete viruses can interact with antibodies to VP7 various antibody types and methodologies were utilized to determine the physical accessibility of the core to the external environment.

Umatilla Virus Association with Fibrils and Tubules in Cultured Cells

1980 ◽  
Vol 38 ◽  
pp. 476-477
Author(s):  
Neil M. Foster ◽  
Ruth D. Breckon
Keyword(s):  
Bluetongue Virus ◽  
Linear Array ◽  
Cultured Cells ◽  
Intimate Association ◽  
Infected Cells ◽  
Dark Staining ◽  
In Cells

Macrotubules have been described1 in cells infected with Umatilla virus (UMAV), an orbivirus for which bluetongue virus (BTV) is the protype. Macrotubules, often in linear array, were observed in the cytoplasm and in intimate association with viroplasms of infected cells. Macrotubules had outside and inside diameters of 20 and 15 nm and many had dark-staining centers with diameters similar to the interiors of the tubules. UMAV was 60 nm and the RNA core was 30 nm in diameter. This report describes the association of UMAV with macrotubules and two types of microtubules.

Molecular basis of bluetongue virus neutralization.

1983 ◽  
Vol 48 (3) ◽  
pp. 627-632 ◽  
Author(s):  
J Kahlon ◽  
K Sugiyama ◽  
P Roy
Keyword(s):  
Molecular Basis ◽  
Bluetongue Virus ◽  
Virus Neutralization

scholarly journals Bluetongue Virus Particles as Nanoreactors for Enzyme Delivery and Cancer Therapy

2021 ◽  
Author(s):  
Eva C. Thuenemann ◽  
Duc H. T. Le ◽  
George P. Lomonossoff ◽  
Nicole F. Steinmetz
Keyword(s):  
Cancer Therapy ◽  
Bluetongue Virus ◽  
Virus Particles ◽  
Enzyme Delivery

scholarly journals Trafficking of Bluetongue Virus Visualized by Recovery of Tetracysteine-Tagged Virion Particles

2014 ◽  
Vol 88 (21) ◽  
pp. 12656-12668 ◽  
Author(s):  
J. Du ◽  
B. Bhattacharya ◽  
T. H. Ward ◽  
P. Roy
Keyword(s):  
Bluetongue Virus ◽  
Virion Particles

A multi-species indirect ELISA for detection group-specific antibodies against VP7 protein of bluetongue virus

2019 ◽  
Vol 180 ◽  
pp. 6-8 ◽  
Author(s):  
Karam Chand ◽  
Sanchay Kumar Biswas ◽  
Muthannan Andavar Ramakrishnan
Keyword(s):  
Bluetongue Virus ◽  
Indirect Elisa ◽  
Specific Antibodies ◽  
Vp7 Protein
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