scholarly journals Estimation of Antioxidant Potential of Indigenous Halari and French Poitu Donkey Milk by using the Total Antioxidant Capacity and Ferric Reducing Antioxidant Power Essay

2019 ◽  
Vol 38 (04) ◽  
Author(s):  
Anuradha Bhardwaj ◽  
Parbha Kumari ◽  
Varij Nayan ◽  
Tanvi Sonali ◽  
R A Legha ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Potential ◽  
Donkey Milk ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Reducing Ability ◽  
Aging Properties ◽  
Total Antioxidant ◽  
Ferric Reducing Antioxidant Power

Over the last decade, research has been conducted towards the uses and properties of donkey milk that has distinct chemical composition and consequently particularly nutritional properties. It is interesting to determine antioxidant potential of indigenous Halari and French Poitu donkey milk by using the total antioxidant capacity and ferric reducing antioxidant power assay. These methods are reliable, fast and with sound methodological infrastructures. The objective of the present research was to investigate the applicability of the trolox based total antioxidant capacity (TAC) and ferric reducing ability of plasma (FRAP) assay for identification of antioxidant potential of Indian Halari donkey milk and French Poitu donkey milk. The donkey milk is quite popular for its anti-aging properties in Western and European countries as cosmetic and nutraceutical preparations. However, in Indian context no such study is conducted on Halari donkey milk. The Indian Halari donkeys are untapped resource for their dairy potential. Our results showed that the whole milk of Halari donkey is at par with French Poitu donkey milk in terms of their antioxidative potential.

Total Antioxidant Capacity of Thai Herbal Teas by the Ferric Reducing Antioxidant Power

2019 ◽  
Vol 886 ◽  
pp. 46-51
Author(s):  
Techaoei Surachai ◽  
Jarmkom Khemjira ◽  
Khobjai Warachate
Keyword(s):  
Vitamin C ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Herbal Tea ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Intermediate Activity ◽  
Total Antioxidant ◽  
Ferric Reducing Antioxidant Power

The main objective aimed to compare in vitro antioxidant power of different recipes of Thai herbal teas including of Tatirot, Krajeab, Kamfoi, and Kesorn Bua. The ferric reducing/antioxidant power (FRAP) assay was used to measure the total antioxidant power of freshly brewed tea. Results showed that different Thai tea recipes had slightly different in vitro antioxidant power. The herbal teas recipe was expressed as µM of antioxidant power/g of dried Thai tea recipes. Values ranges as 555.62±0.77-908.43±0.69 µM/1g of Thai herbal tea, especially Krajeab tea showed strongly antioxidant of 908.43±0.69 µM/1g of tea when compared with other samples. Therefore, it has confirmed that the antioxidant power of Thai herbal tea recipes is considerably intermediate activity than vitamin C

Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay

2019 ◽  
Vol 13 (3) ◽  
pp. 608-616 ◽  
Author(s):  
Andrew Jones ◽  
Agustín Acquaviva ◽  
Thirada Suktham ◽  
Gary R. Dennis ◽  
R. Andrew Shalliker ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Power ◽  
Total Antioxidant ◽  
Ferric Reducing Antioxidant Power

scholarly journals Chemical Characterization and Antioxidant Activity of Cedrus atlantica Manetti Tar (Atlas Cedar Tar)

2020 ◽  
Vol 8 (2) ◽  
pp. 244-255
Author(s):  
Inssaf Skanderi ◽  
Ourida Chouitah
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Condensed Tannins ◽  
Chemical Characterization ◽  
Antioxidant Power ◽  
Cedrus Atlantica ◽  
Total Antioxidant ◽  
Ferric Reducing Antioxidant Power ◽  
Total Polyphenolic Content

Cedrus atlantica Manetti Tar was analyzed by gas chromatography coupled to mass spectrometry, it was subjected to analyzes to know their total polyphenolic and condensed tannins contents, also their Ferric-reducing antioxidant power and Total antioxidant capacity. Chemical characterization identified 88 constituents where Himachalene and α-atlantone isomers (14.51 % - 4.07 %), Calacorene (3.52 %) and ar-Turmerone 3.35 %, were the major components, the total polyphenolic content and condensed tannins contents were 57.15 ± 0.15 milligrams equivalent of gallic acid /g tar and 4.41 ± 0.05 milligrams equivalent of catechin /g tar respectively .This extract showed remarkable Ferric-reducing antioxidant power with effective concentration equal to 50 ± 0.075 mg /mL ± 0,00028 and total antioxidant capacity equal to 262.75 mg equivalents of ascorbic acid /g tar ± 14,43. The experimental results indicated that our tar has promotive antioxidant activity.

scholarly journals Comparison of different analytical methods for assessing total antioxidant capacity of human serum

1998 ◽  
Vol 44 (6) ◽  
pp. 1309-1315 ◽  
Author(s):  
Guohua Cao ◽  
Ronald L Prior
Keyword(s):  
Antioxidant Capacity ◽  
Human Serum ◽  
Total Antioxidant Capacity ◽  
Serum Proteins ◽  
High Specificity ◽  
Fixed Time ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Frap Assay ◽  
Reducing Ability ◽  
Total Antioxidant

Abstract Three assays were compared for the determination of total antioxidant capacity in human serum: the oxygen radical absorbance capacity (ORAC) assay, the Randox Trolox-equivalent antioxidant capacity (Randox-TEAC) assay, and the ferric reducing ability (FRAP) assay. There was a weak but significant linear correlation between serum ORAC and serum FRAP. There was no correlation either between serum ORAC and serum TEAC or between serum FRAP and serum TEAC. The effect of dilution on the serum TEAC value and the use of inhibition percentage at a fixed time, without considering the length of inhibition time in the quantitation of results, adversely affected the Randox-TEAC assay. The FRAP assay is simple and inexpensive but does not measure the SH-group-containing antioxidants. The ORAC assay has high specificity and responds to numerous antioxidants. By utilizing different extraction techniques in the ORAC assay, one can remove serum proteins and also make some gross differentiation between aqueous and lipid-soluble antioxidants. However, the ORAC assay requires ∼60 min more than the FRAP or Randox-TEAC assay to quantitate results.

scholarly journals Study of the Antioxidant Property Variation of Cornelian Cherry Fruits during Storage Using HPTLC and Spectrophotometric Assays

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Anamaria Hosu ◽  
Claudia Cimpoiu ◽  
Luminita David ◽  
Bianca Moldovan
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Antioxidant Compounds ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Property Variation ◽  
Cornelian Cherry ◽  
Cornus Mas ◽  
Total Antioxidant

Cornus species fruits are well known as a rich source of antioxidant compounds responsible for their diverse health benefits. The present study aims to investigate the variation of the total antioxidant capacity of Cornelian cherry (Cornus masL.) fruits during storage, using high-performance thin-layer chromatography (HPTLC) and two spectrophotometric assays based on different mechanisms: the 2,2-azinobis(3-ethylbenzothiazolyne-6-sulphonic acid) radical cation (ABTS+∙) assay and the ferric reducing antioxidant power (FRAP) assay. The fruit extract was stored at room temperature (22°C) for 19 days. No major differences in the total antioxidant capacity were observed during this period, indicating that storage does not have any deleterious effect on the antioxidant properties of the investigated fruits extract. The antioxidant capacity varied between 12.91 and 12.83 µmol Trolox/g fruit as determined by the HPTLC method and from 36.13 to 33.93 µmol Trolox/g fruit as determined by the ABTS assay.

Total Antioxidant Capacity of Feces of Mammalian Herbivores and Carnivores

2014 ◽  
Vol 69 (3-4) ◽  
pp. 165-169 ◽  
Author(s):  
Bogdan Saletnik ◽  
Grzegorz Bartosz ◽  
Janusz Markowski ◽  
Izabela Sadowska-Bartosz
Keyword(s):  
Total Antioxidant Capacity ◽  
Sulfonic Acid ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Mammalian Herbivores ◽  
Reduction Assay ◽  
Total Antioxidant ◽  
Carnivorous Species ◽  
Ferric Reducing Antioxidant Power ◽  
Herbivorous Species

The total antioxidant capacities (TAC) of feces of mammalian herbivores and carnivores were compared. TAC were estimated using three different methods: 2,2’-azino-bis(3-ethylbenzothiazoline- 6-sulfonic acid) radical (ABTS·) reduction, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH·) reduction, and ferric reducing antioxidant power (FRAP). TAC of 18 herbivorous species were generally higher with respect to 16 carnivorous species [(14:21±6:72) vs. (9:45±7:32) mmol Trolox equivalents/kg feces; P < 0:05] in the FRAP assay. The ABTS· reduction assay indicated that the TAC originating from “fast” reacting antioxidants were higher in the herbivores than in carnivores [(17:92±7:18) vs. (12:22±8:5) mmol Trolox equivalents/kg feces; P < 0:05], while a reverse trend was observed for TAC originating from “slowly” reacting antioxidants [(20:68±4:85) vs. (24:68±6:87) mmol Trolox equivalents/kg feces].

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