Clinico-pathologic observations of spontaneous hepatic coccidiosis in broiler rabbits maintained in Bannerghatta biological park in Karnataka state of India

2019 ◽  
Author(s):  
V. Manjunatha ◽  
M. Rout ◽  
C.S Sujay ◽  
N. Jaisingh ◽  
Nikitha Salin ◽  
...  
Keyword(s):  
Bile Duct ◽  
Clinical Signs ◽  
Inflammatory Cells ◽  
Cell Aggregation ◽  
Stunted Growth ◽  
Hepatic Cells ◽  
Karnataka State ◽  
Biochemical Examination ◽  
Eimeria Stiedae ◽  
Hepatic Coccidiosis

The present study reports the clinico-pathologic changes observed in spontaneous hepatic coccidiosis caused by Eimeria stiedae in rabbits at Bannerghatta Biological Park (BBP) in Karnataka state of India. The clinical signs observed in the affected rabbits included sudden death, stunted growth, anorexia, weight loss, diarrhoea and abdominal pain. Increased liver enzymes and bilirubin was noticed upon biochemical examination. At necropsy, lesions included grossly enlarged liver (hepatomegaly) with presence of discrete yellowish-white nodules ranging from 1mm to 5mm size throughout the parenchyma containing a thick creamy white fluid. Faecal sample examination demonstrated the presence of Eimeria oocyts. The impression smears from the liver and intestinal mucosa also showed Eimeria oocysts, degenerative changes, necrotic cells and mononuclear cell aggregation to form oocyst granuloma. Histological observation revealed distension of bile duct, hyperplasia of epithelium along with numerous coccidian oocysts at the centre. Granulomatous tissues were observed to have encircled the bile duct with infiltration of inflammatory cells. Microscopic examination of exudates from liver also revealed numerous oocysts of E. stiedae. Multiple areas of coagulative necrosis of hepatic cells surrounded with inflammatory cells were also found.

scholarly journals Pathological study of Hepatic coccidiosis in naturally infected rabbits

2012 ◽  
Vol 11 (1) ◽  
pp. 63 ◽  
Author(s):  
R. A.S AL- Naimi, O. H. Khalaf, S. Y. Tano And E. H. Al- Taee
Keyword(s):  
Developmental Stages ◽  
Clinical Signs ◽  
Cell Aggregation ◽  
Liver Parenchyma ◽  
Histopathological Changes ◽  
Pathological Study ◽  
Hair Coat ◽  
Pendulous Abdomen ◽  
Eimeria Stiedae ◽  
Hepatic Coccidiosis

Thirty young domesticated rabbits (age 2-2.5 mo) of sexes showed clinical signs of anorexia, debilitation, diarrhea, icterus, rough hair coat and pendulous abdomen with hepatomegaly. Fecal samples were collected for demonstrated the presence of oocysts. Postmortem examination revealed the presence of discrete yellowish-white nodules of 1mm to 2 cm size on the surface and throughout the parenchyma containing a thick creamy white fluid. The histopathological changes showed biliary hyperplasia with different developmental stages of Eimeria stiedae in the epithelial cells, cholangitis and peribiliary fibrosis with newly formed bile ductules, severe congestion, and dilation of central veins and sinusoids with disruption hemorrhagic areas. The hepatocytes showed degenerative changes to necrosis with areas of fibrosis and mononuclear cell aggregation, obstructive jaundice and a tendency to form oocyst granuloma. In conclusion hepatic coccidiosis lead to severe pathological changes both in bile ducts and liver parenchyma especially in young animals.

Lesions of Subclinical Doberman Hepatitis

1998 ◽  
Vol 35 (5) ◽  
pp. 361-369 ◽  
Author(s):  
M. Speeti ◽  
J. Eriksson ◽  
S. Saari ◽  
E. Westermarck
Keyword(s):  
Bile Duct ◽  
Biopsy Specimen ◽  
Clinical Signs ◽  
Inflammatory Cells ◽  
Bile Duct Proliferation ◽  
Two Samples ◽  
Doberman Pinschers ◽  
Initial Biopsy ◽  
Piecemeal Necrosis ◽  
Histologic Changes

This investigation describes histologic lesions in the livers of 18 Doberman Pinschers suffering from subclinical doberman hepatitis (DH). The dogs' ages ranged from 2.5 to 7 years; 15 were females and 3 were males. At the time of liver biopsy, the dogs had no clinical signs of liver disease, although serum alanine aminotransferase (ALT) values had been elevated in two samples in successive months. In the histologic examination, all biopsies revealed parenchymal and portal mononuclear inflammation. In the parenchyma, the inflammation was diffuse, with multifocal clusters of inflammatory cells. The periportal reaction was usually mild to moderate. Bridging necrosis (3/18) and bile duct proliferation (2/18) were rare. Excessive copper was detected by rubeinic acid stain in every specimen. Postmortem liver samples were obtained from nine dogs 3.5–65 months after the initial biopsy specimen; five of these dogs had been euthanatized for reasons other than DH, and liver specimens revealed piecemeal necrosis (5/5), bridging necrosis (3/5), and bile duct proliferation (2/5). Four of them had been euthanatized because of DH. Liver lesions of these dogs were typical for chronic active hepatitis, with bridging and piecemeal necrosis (4/4), portal expansion (4/4), bile duct proliferation (4/4), and fibrosis (4/4). A scoring system was used to evaluate changes numerically from biopsy to postmortem samples. Lesions in all dogs had progressed. The most important histologic changes were expansion of portal areas ( P = 0.008), increased periportal and bridging necrosis ( P = 0.008), increased fibrosis ( P = 0.016), and proliferation of the bile ducts ( P = 0.063).

scholarly journals Proposal of an Algorithm to Early Detect Attenuated Type I Mucopolysaccharidosis (MPS Ia) among Children with Growth Abnormalities

Medicina ◽  
2022 ◽  
Vol 58 (1) ◽  
pp. 97
Author(s):  
Federico Baronio ◽  
Stefano Zucchini ◽  
Francesco Zulian ◽  
Mariacarolina Salerno ◽  
Rossella Parini ◽  
...  
Keyword(s):  
Short Stature ◽  
Metabolic Disorders ◽  
Clinical Presentation ◽  
Diagnostic Delay ◽  
Clinical Signs ◽  
Clinical Approach ◽  
Type I ◽  
Mucopolysaccharidosis Type I ◽  
Stunted Growth ◽  
Growth Abnormalities

Background and Objectives: Diagnostic delay is common in attenuated Mucopolysaccharidosis Type I (MPS Ia) due to the rarity of the disease and the variability of clinical presentation. Short stature and impaired growth velocity are frequent findings in MPS Ia, but they rarely raise suspicion as paediatric endocrinologists are generally poorly trained to detect earlier and milder clinical signs of this condition. Materials and Methods: Following a consensus-based methodology, a multidisciplinary panel including paediatric endocrinologists, paediatricians with expertise in metabolic disorders, radiologists, and rheumatologists shared their experience on a possible clinical approach to the diagnosis of MPS Ia in children with short stature or stunted growth. Results: The result was the formation of an algorithm that illustrates how to raise the suspicion of MPS Ia in a patient older than 5 years with short stature and suggestive clinical signs. Conclusion: The proposed algorithm may represent a useful tool to improve the awareness of paediatric endocrinologists and reduce the diagnostic delay for patients with MPS Ia.

scholarly journals the Study of some histopathological changes occurring in white laboratory mice infected with Cutaneous Leishmaniasis in Al – diwaniyah province, Iraq.

2021 ◽  
Vol 26 (4) ◽  
Author(s):  
Lubna.A.Al-ibrahimi Edu ◽  
Hind Al-shabani ◽  
Marwa sami alwan
Keyword(s):  
Bile Duct ◽  
Mediterranean Region ◽  
Histological Study ◽  
Inflammatory Cells ◽  
Skin Lesions ◽  
Intracellular Parasite ◽  
Laboratory Mice ◽  
Histopathological Changes ◽  
Radial Arrangement ◽  
Dermis Layer

          Leishmaniasis is caused by an intracellular parasite . It is endemic in Asia, Africa, the Americas, and the Mediterranean region. Worldwide, 1.5 to 2 million new cases occur each year .The histological study of the liver tissue of white laboratory mice (Mus musculus) infected with L. major a parasite showed The presence of severe steatosis of hepatocytes Hepatocyte degeneration, And loss of the radial arrangement of hepatocytes, With heavy infiltration in inflammatory cells, especially phagocytes( Macrophage) with  Hyperplasia and congestion of the bile duct  .  As for histological sections of skin lesions taken from ear , Foot , tail ، were showed epidermal ulcerative , Accompanied by severe leaching of the dermis layer neutrophil ,polymorph lymphocytes ، with hemorrhage of ​​the dermis, with necrosis of the epidermal cells of all skin lesions in the ear, foot and tail.

Lens and Anterior Uvea

2022 ◽  
pp. 359-392
Keyword(s):  
Macular Edema ◽  
Anterior Uveitis ◽  
Clinical Signs ◽  
Inflammatory Cells ◽  
Clinical Findings ◽  
Eye Drops ◽  
Systemic Diseases ◽  
Intravitreal Triamcinolone ◽  
Keratic Precipitates ◽  
Intravitreal Triamcinolone Injection

This chapter illustrates photos of clinical signs seen in uveitis and interesting cases of lens pathologies. Anterior uveitis is the inflammation of the iris and the ciliary body. Anterior uveitis can be idiopathic, isolated, or associated with systemic diseases. The clinical findings observed in anterior uveitis include keratic precipitates, inflammatory cells and flare in anterior chamber, hypopyon, rarely hyphema, miosis, iris nodules and atrophy, synechiae, and band keratopathy in chronic cases (shown in corneal degenerations chapter). The inflammation in anterior uveitis is almost always immune. Treatment includes steroid eye drops, cycloplegic drops, sub-Tenon steroid injections when cystoid macular edema is present. Chronic macular edema can be treated with intravitreal Triamcinolone injection and Dexamethasone implants. In cases of refractory anterior uveitis or associated immune systemic diseases, immunomodulatory treatment or biologic agents are prescribed.

scholarly journals Intraductal Papillary Neoplasm of the Bile Duct With Invasive Adenocarcinoma Complicating IgG4-related Sclerosing Cholangitis: Report of a Case

2017 ◽  
Vol 102 (1-2) ◽  
pp. 70-76
Author(s):  
Ryo Ashida ◽  
Teiichi Sugiura ◽  
Yukiyasu Okamura ◽  
Takaaki Ito ◽  
Keiko Sasaki ◽  
...  
Keyword(s):  
Bile Duct ◽  
Sclerosing Cholangitis ◽  
Invasive Carcinoma ◽  
Extrahepatic Bile Duct ◽  
Inflammatory Cells ◽  
Wall Thickening ◽  
Multidetector Row Computed Tomography ◽  
Intraductal Papillary Neoplasm ◽  
Serum Igg4 ◽  
Papillary Neoplasm

Although there have been many previous studies of IgG4-related SC focusing on the differential diagnosis from cholangiocarcinoma, only a few patients with cholangiocarcinoma against a background of IgG4-related SC have been reported. We herein present a case of intraductal papillary neoplasm of the bile duct (IPNB) associated with invasive carcinoma complicating IgG4-related sclerosing cholangitis. A 71-year-old female with icterus was admitted to a local hospital, where stricture of the extrahepatic bile duct were detected, and subsequently referred to our hospital for possible surgery. Abdominal multidetector-row computed tomography demonstrated marked wall thickening along the entire extrahepatic bile duct. The left lateral superior bile duct (B2) and left lateral inferior duct (B3) were individually obstructed, and percutaneous transhepatic biliary drainage catheters were placed in B2 and B3 separately. The patient was diagnosed to have diffusely spread cholangiocarcinoma and underwent right hepatic trisectionectomy with caudate lobectomy and pancreatoduodenectomy. A histological examination revealed intraductal papillary tumors composed of fibrovascular stalks covered by neoplastic epithelium. Carcinomatous invasion of the papillary tumors was observed in the fibromuscular layer, and there was abundant infiltration of inflammatory cells with fibrosis outside of the cancerous tissue. The inflammatory cells were primarily composed of plasma cells, a majority of which were positive for IgG4 (>30 cells/high-power field); the postoperative serum IgG4 level was 890 mg/dL. Therefore, a diagnosis of coexisting IPNB associated with invasive carcinoma and IgG4-related sclerosing cholangitis was made. To the best of our knowledge, this is the first report of IPNB complicating IgG4-related sclerosing cholangitis.

scholarly journals Leukoencephalomalacia in horses associated with immature corn consumption

2019 ◽  
Vol 49 (3) ◽  
Author(s):  
Joanna Vargas Zillig Echenique ◽  
Pablo Estima-Silva ◽  
Daniela Brayer Pereira ◽  
Lucas Santos Marques ◽  
Luiza Soares Ribeiro ◽  
...  
Keyword(s):  
White Matter ◽  
Clinical Signs ◽  
Inflammatory Cells ◽  
Cell Swelling ◽  
Fusarium Spp ◽  
Column Method ◽  
Reproductive Phase ◽  
Eosinophilic Cytoplasm ◽  
Abundant Eosinophilic Cytoplasm ◽  
In The Beginning

ABSTRACT: Horse leukoencephalomalacia (ELEM) is a disease caused by the ingestion of mycotoxins (fumonisins) produced by fungi of the genus Fusarium that infect corn and/or its byproducts. This disease has been described by ingestion of mature corn with humidity above 15% at temperatures below 20°C. The aim of this paper was to report an outbreak of leukoencephalomalacia in horses fed with immature corn. Two horses out of three showed neurological signs approximately seven days after eating immature corn in its reproductive phase (R2, milky grains). Corn was harvested and administered directly to the animals, with no storage. Deaths occurred approximately 24 hours after the onset of clinical signs. Grossly, there were multifocal dark red to brown areas in the white matter of the telencephalon and hyppocampus and thalamus. Histologically, there was edema and hemorrhage in several areas of the telencephalon white matter, which corresponded to dark red to brown areas observed in the macroscopy. There was also foci of malacia with presence of reactive astrocytes with abundant eosinophilic cytoplasm and inflammatory cells. Diffuse capillary wall degeneration and endothelial cell swelling were also observed. Two ppm of fumonisin were detected by immunoaffinity column method (VICAM) in the immature corn sample. The water activity in this cereal, when the grain is still milky, is 0.98 and can predispose it to growth of mycotoxin-producing fungi. In the present case, fumonisin was found in milky grains in the beginning of the reproductive phase (R2), which suggested that even immature corn may be infected by Fusarium spp. and should not be administered to horses.

Simultaneous segmental obstruction of bile duct and portal vein markedly changes a population of biliary and hepatic cells in human liver

2003 ◽  
Vol 388 (4) ◽  
pp. 270-275 ◽  
Author(s):  
Takanori Kyokane ◽  
Masato Nagino ◽  
Junichi Kamiya ◽  
Yuji Nimura ◽  
Tetsuro Nagasaka
Keyword(s):  
Bile Duct ◽  
Portal Vein ◽  
Human Liver ◽  
Hepatic Cells

Potential Side Effects of Dendritic Cells Pulsed with Allogenic Melanoma Cell Lysate in Mice

2007 ◽  
Vol 26 (1) ◽  
pp. 33-40
Author(s):  
Shin-Young Park ◽  
Woo Hyuck Choi ◽  
Yong Bum Kim ◽  
Chang Su Ha ◽  
Hyunah Lee ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Melanoma Cell ◽  
White Blood Cells ◽  
Clinical Signs ◽  
Inflammatory Cells ◽  
Application Site ◽  
Test Substance ◽  
Tunica Media ◽  
Body Weights ◽  
Pulmonary Arteriole

An attempt has been made to investigate the toxicity of cancer immunotherapy based on the dendritic cells pulsed with lysate of allogenic melanoma cell, DM401. Dendritic cells pulsed with lysate of clone M3 were subcutaneously administered once a week eight times to C57BL/6 mice at 0, 2.5, 5, and 10 × 107 cells/kg. No changes attributable to the administration were observed in clinical signs and food and water consumption. The administration induced slight increases in body weights, white blood cells, total protein, total cholesterol, triglyceride, phospholipids, and absolute spleen weights, but a slight decrease in albumin/globulin ratio. Microscopic examinations revealed the infiltration of inflammatory cells in the lung, mainly in the pulmonary arteriole, in which the tunica media thickened, and in the pulmonary alveoli and alveolar space. Thickened tunica media of pulmonary arteriole was observed in both males and females at all selected doses. In addition, the subcutis at the test substance-application site showed inflammation and fibrosis. In conclusion, lung is a target organ of DM401, and most of the changes including the findings in lung are considered as the immunomodulatory functions of dendritic cells.

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